1. Pretreatment with recombinant human interleukin 2 (rhIL-2) Up-regulates PCNA-positive cells after partial hepatectomy in rat liver.
- Author
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Jeon, Miae, Kwon, Hyun, Kim, Yong, Han, Kook-Il, Nam, Kung-Woo, Baik, Yeongjun, Lee, Sunghee, Kim, Wan-Jong, and Han, Man-Deuk
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INTERLEUKIN-2 ,RECOMBINANT proteins ,PROLIFERATING cell nuclear antigen ,HEPATECTOMY ,LIVER regeneration ,LABORATORY rats - Abstract
We prepared recombinant human interleukin-2 (rhIL-2) and studied its pretreated influence on liver regeneration and the blood profile in partially (67%) hepatectomized (PH) male Sprague-Dawley rats. Rats were injected in the tail vein with rhIL-2 three times per day for 3 consecutive days and 67% underwent a partial hepatectomy (PH). Five days after the PH, liver tissue and blood samples were analyzed for liver regeneration and hematological changes. The weight of the liver in the rhIL-2 pretreated groups increased in a dose-dependent manner; with the highest treatment (24 × 10 IU/kg), the maximum liver weight of 88.6% was exhibited. The control group showed a gradual increase to 76.3% of the original liver weight. A histological analysis of the liver showed an increase in proliferating cell nuclear antigen (PCNA)-positive cells in rhIL-2 pretreated rat livers. The rate of hepatocyte proliferation also increased significantly in primary cultured rat liver cells following rhIL-2 treatment. These results suggest that pretreatment with rhIL-2 may play adjuvant roles in liver regeneration after PH. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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