1. Development and application of tumor-targeting magnetic nanoparticles FA-StNP@Fe2O3 for hyperthermia.
- Author
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Zheng YuanQing, Tong ChunYi, Wang Bei, Xie Ying, Liao HongDong, Li Dan, and Liu XuanMing
- Subjects
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NANOPARTICLES , *CANCER cells , *FOLIC acid , *EMULSIONS , *TRANSMISSION electron microscopy , *ZETA potential , *HELA cells - Abstract
Modified magnetic starch nanoparticles (FA-StNP@Fe2O3) were synthesized by conjugating folic acid (FA-PEG-NH2) onto the surface of magnetic starch nanoparticles (StNP@Fe2O3) prepared by reverse microemulsion method. The synthesized FA-StNP@Fe2O3 was investigated by transmission electron microscopy and zeta potential analysis. The average size of its well dispersed particles was 250 nm. The iron concentration of 2 mg/g was detected by phenanthroline method. Placing FA-StNP@Fe2O3 nanoparticles in the alternating magnetic field for 30 min resulted in an increase in the suspension temperature from ambient temperature (37°) to a value between 42° and 43°. Co-cultured nanoparticles and Hela cell line or normal HUEC-12 cell line, and the biological effects at the cellular level were investigated in the alternating magnetic field using MTT assay, Hochest-PI double staining and flow cytometry analysis. Experimental results showed that FA-StNP@Fe2O3 within acertain concentration range has no obvious effect on cell proliferation. When treated in the magnetic field, apoptosis rate on Hela induced by FA-StNP@Fe2O3 was 13.4%. Prussian blue staining analysis confirmed that the nanoparticles modified with folic acid had improved ability in tumor cell-targeting, and therefore, potential applications in biomedical and magnetocaloric areas. It is expected be applied in tumor targeting therapy in the near future. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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