1. Hemin@carbon dot hybrid nanozymes with peroxidase mimicking properties for dual (colorimetric and fluorometric) sensing of hydrogen peroxide, glucose and xanthine.
- Author
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Su, Li, Cai, Yexi, Wang, Liang, Dong, Wenpei, Mao, Guojiang, Li, Ye, Zhao, Mingsheng, Ma, Yanhua, and Zhang, Hua
- Subjects
PEROXIDASE ,GLUCOSE ,OXIDATIVE coupling ,FLUORESCENT probes ,XANTHINE ,DETECTION limit - Abstract
The multifunctional hemin@carbon dot hybrid nanozymes (hemin@CD) with simultaneous peroxidase-like activity and fluorescence signalling property was prepared for the first time. Based on these properties, hemin@CD was applied to develop a dual-channel fluorescent probe for H
2 O2 and H2 O2 -based biocatalytic systems. By virtue of the peroxidase-like activity, hemin@CD can catalyze the oxidative coupling of 4-aminoantipyrine with phenol in the presence of H2 O2 to form a pink-red quinoneimine dye with a maximum absorbance at 505 nm. Under the excitation wavelength of 480 nm, the green fluorescence of hemin@CD peaks at 540 nm and is quenched by the generated quinoneimine dye due to an inner filter effect, and also by H2 O2 because of dynamic quenching. Thus, a colorimetric and fluorimetric dual-channel optical probe for H2 O2 is obtained. Due to the glucose/xanthine transformations under formation of H2 O2 by the relevant oxidase catalysis, the probe can be applied for detection of glucose and xanthine. The colorimetric detection limits for H2 O2 , glucose and xanthine are 0.11, 0.15, 0.11 μM, and the and fluorimetric detection limits are 0.15, 0.15, 0.12 μM, respectively. [ABSTRACT FROM AUTHOR]- Published
- 2020
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