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The remobilization, migration, precipitation, and enrichment of ore-forming elements are closely related to structures. Therefore, detailed regional and ore-field structural analyses are critical for determining the genesis of a mineral deposit. The Jiangnan Orogenic Belt (JOB) is an important gold polymetallic metallogenic belt in South China, which is characterized by multiple periods of gold mineralization in the Paleozoic and Mesozoic. However, the genesis of these gold polymetallic deposits is still not well understood due to a lack of systematic research on the regional geology, ore-controlling structures and metallogenic mechanism. In this study, a detailed structural survey at the surface and in the subsurface tunnels was conducted on the Woxi Au-Sb-W deposit, the genesis of which is relatively controversial among the gold polymetallic deposits in the JOB due to poor structural constraints. In addition, a wolframite U-Pb dating was carried out to further constrain the relationship between structures and mineralization. Based on the results of these studies, together with those from previous studies, it is proposed that the Woxi deposit and surrounding areas likely underwent six periods of regional deformation, which are constrained in time and geodynamic setting. Furthermore, we present a systematic discussion on the roles of ore-controlling structures in the transportation, distribution, and deposition of ore-forming elements and localization of orebodies. According to the wolframite dating results, structural analyses, and previous data, we propose that the Woxi Au-Sb-W deposit was formed in two stages during the Yanshanian: a W (wolframite)-Au mineralization stage at ca. 140 Ma and an Au-Sb-W (scheelite) mineralization stage at <130 Ma. These mineralizing events are interpreted to have a tight relationship with tectonic reactivation, and the ore-forming fluids were derived from deep sources, including those of magmatic or metamorphic origins. The Woxi deposit can therefore be classified as an "intracontinental reactivation-type", and the mineralization is related to lithospheric extension caused by plate retreat, retention, and delamination following the cessation of westward subduction of the Paleo-Pacific Plate beneath the East Asian continent. [ABSTRACT FROM AUTHOR]