1. Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea.
- Author
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Lee, Yu and Lee, Changhee
- Subjects
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PARAINFLUENZA viruses , *VIRUS isolation , *NUCLEOTIDE sequence , *VIRAL genomes , *PARAMYXOVIRUSES , *ALVEOLAR macrophages - Abstract
A novel cytopathogenic paramyxovirus was isolated from a lung sample from a piglet, using continuous porcine alveolar macrophage cells. Morphologic and genetic studies indicated that this porcine virus (pPIV5) belongs to the species Parainfluenza 5 in the family Paramyxoviridae. We attempted to determine the complete nucleotide sequence of the first Korean pPIV5 isolate, designated KNU-11. The full-length genome of KNU-11 was found to be 15,246 nucleotides in length and consist of seven nonoverlapping genes (3′-N-V/P-M-F-SH-HN-L-5′) predicted to encode eight proteins. The overall degree of nucleotide sequence identity was 98.7 % between KNU-11 and PIV5 (formerly simian virus 5, SV5), a prototype paramyxovirus, and the putative proteins had 74.4 to 99.2 % amino acid identity to those of PIV5. Phylogenetic analysis further demonstrated that the novel pPIV5 isolate is a member of the genus Rubulavirus of the subfamily Paramyxovirinae. The present study describes the identification and genomic characterization of a pPIV5 isolate in South Korea. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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