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Acaricide residues were assessed in French commercial beeswax using newly developed liquid and gas chromatography methods. Most of the commercial wax samples and all samples taken during the industrial recycling process contained coumaphos and fluvalinate. Amitraz and coumaphos residue levels were also followed in several hives experimentally treated with Asuntol?50 or Apivar?, two products used in France to control varroa infestation. After the Asuntol?50 treatment, coumaphos residues increased in honey and wax combs, persisted more than 30 days in honey and one year or more in comb wax. The half-life of coumaphos was 69 and 115?346 days in honey and comb wax respectively. Following Apivar?treatment, amitraz was not detected in honey nor in wax. These results are consistent with and complete other studies: the use of coumaphos entails wax contamination which persists through commercial recycling. As this may be a threat for bee health, the use of Asuntol?50 should be avoided. [ABSTRACT FROM AUTHOR]