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The CALorimetric Electron Telescope CALET is collecting science data on the International Space Station since October 2015 with excellent and continuous performance. Energy is measured with a deep homogeneous calorimeter (1.2 nuclear interaction lengths, 27 radiation lengths) preceded by an imaging pre-shower (3 radiation lengths, 1mm granularity) providing tracking and electron/proton discrimination. Two independent sub-systems identify the charge of the incident particle from proton to iron and above ( 40). CALET measures the cosmic-ray electron + positron flux up to 20 TeV, gamma rays up to 10 TeV, and nuclei up to the PeV scale. In this paper, we report the on-orbit performance of the instrument and summarize the main results obtained during the first 5 years of operation, including the electron + positron energy spectrum and the individual spectra of protons, heavier nuclei and iron. Solar modulation and gamma-ray observations are also concisely reported, as well as transient phenomena and the search for gravitational wave counterparts. [ABSTRACT FROM AUTHOR]

Abrasion of the brake lining of automobiles is one of the main antimony (Sb) sources on the road. Therefore, the road effluent possibly supplies Sb to the combined sewer collection system. However, Sb in road-sewer systems has attracted little concern, although heavy metals such as copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) in sewer water have been studied by many previous authors. We investigated the effect of road effluent on Sb in the combined sewer water by collecting road effluent, road dust, and sewer water under rainy and dry weather conditions. Sb in road effluent showed a significantly higher concentration than the other types of samples, and the Sb concentration in sewer during wet weather was significantly higher than that during dry weather. Furthermore, the Sb concentration in sewer water decreased with time during a wash-off event. Clear positive relationships between Sb and Cu and between Sb and Ba in both road effluent and road dust extract indicate the effect of brake abrasion because the brake lining contains Cu, Sb, and Ba in high concentrations. Approximately 42% of Sb load occurred during the wash-off event, while the loads of Cu and Ba were much less. Unlike Cu and Ba, we conclude that Sb in combined sewer water largely depends on road effluent in wet weather due to the wash-off of road dust, which is probably associated with brake lining abrasion. [ABSTRACT FROM AUTHOR]

The CALorimetric Electron Telescope (CALET) is an astroparticle physics experiment installed on the International Space Station since August 2015. The CALET mission was conceived to address several outstanding questions of high-energy astroparticle physics, like indirect detection of dark matter, the origin of cosmic rays (CRs), their mechanisms of acceleration and galactic propagation, the presence of possible nearby astrophysical CR sources. That can be achieved by precise measurements of the fluxes of CR electrons and γ rays up to the unexplored region above 1 TeV, and the energy spectra and composition of CR nuclei from a few tens of GeV to hundreds of TeV. In order to perform these observations, the instrument combines a thick total absorption PWO crystal calorimeter for energy measurement, a scintillator hodoscope for charge identification and thin imaging tungsten-scintillating fiber calorimeter providing accurate particle tracking and complementary charge measurement. In this paper, we will present an overview of the main CALET results based on the data collected in the first three years of the mission. [ABSTRACT FROM AUTHOR]

Hepatitis B virus X protein (HBx) has many cellular functions and is a major factor in hepatitis and hepatocellular carcinoma caused by HBV infection. A proteomic approach was used to search for HBx-interacting proteins in order to elucidate the molecular mechanism of hepatocarcinogenesis. HBx was attached to myc and flag tags (MEF tags) and expressed in 293T cells; the protein complex formed within the cells was purified and characterized by mass spectrometry. COP9 signalosome (CSN) subunits 3 and 4 were subsequently identified as HBx-interacting proteins. In addition, CSN subunit 5, Jun activation domain-binding protein 1 (Jab1), was shown to be a novel cellular target of HBx. In vivo and in vitro interactions between HBx and Jab1 were confirmed by standard immunoprecipitation and GST pull-down assays. An analysis of HBx deletion constructs showed that amino acids 30–125 of HBx were responsible for binding to Jab1. Confocal laser microscopy demonstrated that HBx was mainly localized in the cytoplasm, while Jab1 was found mainly in the nucleus and partially in the cytoplasm, and that the two proteins colocalized in the cytoplasm. The cotransfection of HBx and Jab1 resulted in substantial activator protein 1 (AP-1) activation and knockdown of endogenous Jab1 attenuated AP-1 activation caused by HBx. In addition, the coexpression of HBx and Jab1 potentiated phosphorylation of JNK, leading to the subsequent phosphorylation of c-Jun, whereas the level of c-Jun and JNK phosphorylation induced by HBx was decreased in Jab1 knockdown cells. These results suggest that the interaction between HBx and Jab1 enhances HBx-mediated AP-1 activation.Oncogene (2006) 25, 633–642. doi:10.1038/sj.onc.1209093; published online 10 October 2005 [ABSTRACT FROM AUTHOR]

A recent study revealed that the p110α (PIK3CA), catalytic subunit of phosphatidylinositol 3-kinase (PI3K), is somatically mutated in many types of cancer. For example, PIK3CA is mutated in an estimated 35.6% of hepatocellular carcinoma (HCC) cases. To measure the frequency of PIK3CA hotspot mutations in Japanese HCC patients, exons 9 and 20 of the PIK3CA gene were sequenced in 47 clinical HCC samples. Contrary to expectations, no hotspot mutations were found any of the HCC samples. In addition, we found abnormally migrating waves near the end of exon 9 in the PCR chromatograms from 13 of the 47 samples. PCR amplification and subsequent cloning and sequencing revealed that these chromatograms contained two distinct sequences, the wild-type p110α sequence and a different sequence found on human chromosome 22q11.2, the Cat Eye Syndrome region, which contains a putative pseudogene of PIK3CA. These abnormally migrating waves were also found in noncancerous liver tissue, indicating that this was not a result of HCC-associated mutations. Therefore, it is likely that the percentage of hotspot mutations in the PIK3CA gene of Japanese HCC patients is lower than was previously reported.Oncogene (2006) 25, 2950–2952. doi:10.1038/sj.onc.1209311; published online 12 December 2005 [ABSTRACT FROM AUTHOR]