42 results on '"BI Yun"'
Search Results
2. New insights on intestinal microorganisms and carbohydrate metabolism in fish.
- Author
-
Zhang, Bi-Yun, Cai, Guo-He, Yang, Hong-Ling, Nie, Qing-Jie, Liu, Zi-Yan, and Sun, Yun-Zhang
- Subjects
- *
CARBOHYDRATE metabolism , *MICROBIAL metabolism , *INTESTINES , *METABOLIC disorders , *FISH diseases , *NUTRITION - Abstract
Fish intestinal tract harbors numerous microorganisms, which exert a critical function in maintaining intestinal health and regulating nutrition metabolism. Although the most economical energy source, carbohydrates cannot be efficiently utilized by some carnivorous fish and even bring serious metabolic diseases. Therefore, how to improve carbohydrate utilization is an essential direction in fish nutrition research. It is well acknowledged that intestinal microorganisms play a crucial part in the host's growth, digestion, metabolism, and immunity, but the information about their roles in fish carbohydrate metabolism is very limited. Here, we review the progress of intestinal microorganisms and carbohydrate metabolism in fish, focusing on carbohydrate and fish health, carbohydrate metabolism disorders and the possible mechanisms, and the roles of intestinal microorganisms in carbohydrate metabolism and relative metabolic disease control. This review provides theoretical support for improving the utilization of carbohydrates and preventing relative metabolic diseases in fish from the perspective of intestinal microorganisms. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. High dietary wheat starch negatively regulated growth performance, glucose and lipid metabolisms, liver and intestinal health of juvenile largemouth bass, Micropterus salmoides.
- Author
-
Zhang, Bi-Yun, Yang, Hong-Ling, Nie, Qing-Jie, Zhang, Yu, Cai, Guo-He, and Sun, Yun-Zhang
- Abstract
Largemouth bass (Micropterus salmoides) were fed with three diets containing 6%, 12%, and 18% wheat starch for 70 days to examine their impacts on growth performance, glucose and lipid metabolisms, and liver and intestinal health. The results suggested that the 18% starch group inhibited the growth, and improved the hepatic glycogen content compared with the 6% and 12% starch groups (P < 0.05). High starch significantly improved the activities of glycolysis-related enzymes, hexokinase (HK), glucokinase (GK), phosphofructokinase (PFK), and pyruvate kinase (PK) (P < 0.05); promoted the mRNA expression of glycolysis-related phosphofructokinase (pfk); decreased the activities of gluconeogenesis-related enzymes, pyruvate carboxylase (PC), and phosphoenolpyruvate carboxykinase (PEPCK); and reduced the mRNA expression of gluconeogenesis-related fructose-1,6-bisphosphatase-1(fbp1) (P < 0.05). High starch reduced the hepatic mRNA expressions of bile acid metabolism–related cholesterol hydroxylase (cyp7a1) and small heterodimer partner (shp) (P < 0.05), increased the activity of hepatic fatty acid synthase (FAS) (P < 0.05), and reduced the hepatic mRNA expressions of lipid metabolism–related peroxisome proliferator–activated receptor α (ppar-α) and carnitine palmitoyltransferase 1α (cpt-1α) (P < 0.05). High starch promoted inflammation; significantly reduced the mRNA expressions of anti-inflammatory cytokines transforming growth factor-β1 (tgf-β1), interleukin-10 (il-10), and interleukin-11β (il-11β); and increased the mRNA expressions of pro-inflammatory cytokine tumor necrosis factor-α (tnf-α), interleukin-1β (il-1β), and interleukin-8 (il-8) in the liver and intestinal tract (P < 0.05). Additionally, high starch negatively influenced the intestinal microbiota, with the reduced relative abundance of Trichotes and Actinobacteria and the increased relative abundance of Firmicutes and Proteobacteria. In conclusion, low dietary wheat starch level (6%) was more profitable to the growth and health of M. salmoides, while high dietary starch level (12% and 18%) could regulate the glucose and lipid metabolisms, impair the liver and intestinal health, and thus decrease the growth performance of M. salmoides. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Long-term outcome of children with acute promyelocytic leukemia: a randomized study of oral versus intravenous arsenic by SCCLG-APL group.
- Author
-
Huang, Dan-Ping, Yang, Liang-Chun, Chen, Yi-Qiao, Wan, Wu-Qing, Zhou, Dun-Hua, Mai, Hui-Rong, Li, Wan-Li, Yang, Li-Hua, Lan, He-Kui, Chen, Hui-Qin, Guo, Bi-Yun, Zhen, Zi-Jun, Liu, Ri-Yang, Chen, Guo-Hua, Feng, Xiao-Qin, Liang, Cong, Wang, Li-Na, Li, Yu, Luo, Jie-Si, and Fan, Zhong
- Subjects
ACUTE promyelocytic leukemia ,CHINESE medicine ,ARSENIC ,CARDIOTOXICITY ,CHILD patients - Abstract
Realgar-Indigo naturalis formula (RIF), an oral traditional Chinese medicine mainly containing Realgar (As
4 S4 ), is highly effective in treating adult acute promyelocytic leukemia (APL). However, the treatment efficacy and safety of RIF have not been verified in pediatric patients. SCCLG-APL group conducted a multicenter randomized non-inferiority trial to determine whether intravenous arsenic trioxide (ATO) can be substituted by oral RIF in treating pediatric APL. Of 176 eligible patients enrolled, 91 and 85 were randomized to ATO and RIF groups, respectively. Patients were treated with the risk-adapted protocol. Induction, consolidation, and 96-week maintenance treatment contained all-trans-retinoic acid and low-intensity chemotherapy, and either ATO or RIF. The primary endpoint was 5-year event-free survival (EFS). The secondary endpoints were adverse events and hospital days. After a median 6-year follow-up, the 5-year EFS was 97.6% in both groups. However, the RIF group had significantly shorter hospital stays and lower incidence of infection and tended to have less cardiac toxicity. All 4 relapses occurred within 1.5 years after completion of maintenance therapy. No long-term arsenic retentions were observed in either group. Substituting oral RIF for ATO maintains treatment efficacy while reducing hospitalization and adverse events in treating pediatric APL patients, which may be a future treatment strategy for APL. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
5. An efficient numerical method on modified space-time sparse grid for time-fractional diffusion equation with nonsmooth data.
- Author
-
Zhu, Bi-Yun, Xiao, Ai-Guo, and Li, Xue-Yang
- Subjects
- *
HEAT equation , *SPACETIME , *DEGREES of freedom , *MULTILEVEL models - Abstract
In this paper, we focus on developing a high efficient algorithm for solving d-dimension time-fractional diffusion equation (TFDE). For TFDE, the initial function or source term is usually not smooth, which can lead to the low regularity of exact solution. And such low regularity has a marked impact on the convergence rate of numerical method. In order to improve the convergence rate of the algorithm, we introduce the space-time sparse grid (STSG) method to solve TFDE. In our study, we employ the sine basis and the linear element basis for spatial discretization and temporal discretization, respectively. The sine basis can be divided into several levels, and the linear element basis can lead to the hierarchical basis. Then, the STSG can be constructed through a special tensor product of the spatial multilevel basis and the temporal hierarchical basis. Under certain conditions, the function approximation on standard STSG can achieve the accuracy order O (2 - J J) with O (2 J J) degrees of freedom (DOF) for d = 1 and O (2 Jd) DOF for d > 1 , where J denotes the maximal level of sine coefficients. However, if the solution changes very rapidly at the initial moment, the standard STSG method may reduce accuracy or even fail to converge. To overcome this, we integrate the full grid into the STSG, and obtain the modified STSG. Finally, we obtain the fully discrete scheme of STSG method for solving TFDE. The great advantage of the modified STSG method can be shown in the comparative numerical experiment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
6. An innovative option for the activation of chalcopyrite flotation depressed in a high alkali solution with the addition of acid mine drainage.
- Author
-
Bai, Shao-jun, Li, Jie, Yuan, Jia-qiao, Bi, Yun-xiao, Ding, Zhan, Dai, Hui-xin, and Wen, Shu-ming
- Abstract
Copyright of Journal of Central South University is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
7. Apatinib plus vinorelbine versus vinorelbine for metastatic triple-negative breast cancer who failed first/second-line treatment: the NAN trial.
- Author
-
Li, Dou-Dou, Tao, Zhong-hua, Wang, Bi-Yun, Wang, Lei-Ping, Cao, Jun, Hu, Xi-Chun, and Zhang, Jian
- Published
- 2022
- Full Text
- View/download PDF
8. Focus on temporal graph convolutional networks with unified attention for skeleton-based action recognition.
- Author
-
Gao, Bing-Kun, Dong, Le, Bi, Hong-Bo, and Bi, Yun-Ze
- Subjects
CONVOLUTIONAL neural networks ,HUMAN behavior ,MACHINE learning - Abstract
Graph convolutional networks (GCN) have received more and more attention in skeleton-based action recognition. Many existing GCN models pay more attention to spatial information and ignore temporal information, but the completion of actions must be accompanied by changes in temporal information. Besides, the channel, spatial, and temporal dimensions often contain redundant information. In this paper, we design a temporal graph convolutional network (FTGCN) module which can concentrate more temporal information and properly balance them for each action. In order to better integrate channel, spatial and temporal information, we propose a unified attention model of the channel, spatial and temporal (CSTA). A basic block containing these two novelties is called FTC-GCN. Extensive experiments on two large-scale datasets, compared with 17 methods on NTU-RGB+D and 8 methods on Kinetics-Skeleton, show that for skeleton-based human action recognition, our method achieves the best performance. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
9. Responsible genes in children with primary vesicoureteral reflux: findings from the Chinese Children Genetic Kidney Disease Database.
- Author
-
Liu, Jia-Lu, Shen, Qian, Wu, Ming-Yan, Zhu, Guang-Hua, Li, Yu-Feng, Wang, Xiao-Wen, Tang, Xiao-Shan, Bi, Yun-Li, Gong, Yi-Nv, Chen, Jing, Fang, Xiao-Yan, Zhai, Yi-Hui, Wu, Bing-Bing, Li, Guo-Min, Sun, Yu-Bo, Gao, Xiao-Jie, Liu, Cui-Hua, Jiang, Xiao-Yun, Hao, Sheng, and Kang, Yu-Lin
- Abstract
Background: Primary vesicoureteral reflux (VUR) is a common congenital anomaly of the kidney and urinary tract (CAKUT) in childhood. The present study identified the possible genetic contributions to primary VUR in children. Methods: Patients with primary VUR were enrolled and analysed based on a national multi-center registration network (Chinese Children Genetic Kidney Disease Database, CCGKDD) that covered 23 different provinces/regions in China from 2014 to 2019. Genetic causes were sought using whole-exome sequencing (WES) or targeted-exome sequencing. Results: A total of 379 unrelated patients (male: female 219:160) with primary VUR were recruited. Sixty-four (16.9%) children had extrarenal manifestations, and 165 (43.5%) patients showed the coexistence of other CAKUT phenotypes. Eighty-eight patient (23.2%) exhibited impaired renal function at their last visit, and 18 of them (20.5%) developed ESRD at the median age of 7.0 (IQR 0.9–11.4) years. A monogenic cause was identified in 28 patients (7.39%). These genes included PAX2 (n = 4), TNXB (n = 3), GATA3 (n = 3), SLIT2 (n = 3), ROBO2 (n = 2), TBX18 (n = 2), and the other 11 genes (one gene for each patient). There was a significant difference in the rate of gene mutations between patients with or without extrarenal complications (14.1% vs. 6%, P = 0.035). The frequency of genetic abnormality was not statistically significant based on the coexistence of another CAKUT (9.6% vs. 5.6%, P = 0.139, Chi-square test) and the grade of reflux (9.4% vs. 6.7%, P = 0.429). Kaplan–Meier survival curve showed that the presence of genetic mutations did affect renal survival (Log-rank test, P = 0.01). PAX2 mutation carriers (HR 5.1, 95% CI 1.3–20.0; P = 0.02) and TNXB mutation carriers (HR 20.3, 95% CI 2.4–168.7; P = 0.01) were associated with increased risk of progression to ESRD. Conclusions: PAX2, TNXB, GATA3 and SLIT2 were the main underlying monogenic causes and accounted for up to 46.4% of monogenic VUR. Extrarenal complications and renal function were significantly related to the findings of genetic factors in children with primary VUR. Like other types of CAKUT, several genes may be responsible for isolated VUR. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
10. zeo19: A thermodynamic database for assessing zeolite stability during the corrosion of nuclear waste immobilization glasses.
- Author
-
Zhen-Wu, Bi Yun, Prentice, Dale P., Ryan, Joseph V., Ellison, Kirk, Bauchy, Mathieu, and Sant, Gaurav
- Subjects
THERMODYNAMICS ,ZEOLITES ,RADIOACTIVE wastes ,THERAPEUTIC immobilization ,PHASE equilibrium - Abstract
Stable, durable immobilization of radioactive wastes requires robust understanding of the sub-surface geochemical processes that occur in repository environments. For example, the accelerated dissolution (corrosion) of nuclear waste immobilization glasses (i.e., the so-called "Stage III" corrosion) following the precipitation of zeolitic phases is a significant issue that could result in radionuclide release. However, current uncertainties in establishing the tendency for the persistence of zeolites results in difficulties in estimating the chemical environments and state variables that favor zeolite precipitation. To assess the tendency for Stage III corrosion, we compiled a unified, internally-consistent thermodynamic database to estimate zeolite stability under conditions relevant to nuclear waste repositories (namely, p = 1 bar and T < 95 °C), i.e., for compounds including: analcime, clinoptilolite, mordenite, erionite, thomsonite, bikitaite, brewsterite, dachiardite, epistilbite, ferrierite, gonnardite, harmotome, leonhardite, paranatrolite, tetranatrolite, yugawaralite zeolite X, and zeolite P(Ca). The database, which features both existing and/or newly-derived thermodynamic properties, is integrated with a Gibbs (free) energy minimization (GEM) solver to estimate stable zeolite phase equilibria and their partitioning at equilibrium. The database offers favorable predictions of the solubility of the zeolite phases as a function of temperature. The validity of the database is ascertained by comparing newly-constructed equilibrium activity diagrams with experimental observations of zeolite formation during glass dissolution across conditions encompassing diverse solution chemistries, pH's, and temperatures. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
11. Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana.
- Author
-
Bi, Yun-Feng, Wang, Xi-Zhu, Jiang, Shan, Liu, Jing-Sheng, Zheng, Ming-Zhu, and Chen, Ping
- Subjects
ESCHERICHIA coli ,BIOCONVERSION ,GINSENOSIDES ,GLUCOSIDASES ,MOLECULAR weights - Abstract
Objectives: To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359. Results: Recombinant β-glucosidase was purified, which molecular weight was about 79.5 kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2 mg ml
−1 ginsenoside, 25 U ml−1 enzyme, 85 °C, and pH 5.0. β-glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3 h under the given conditions, respectively. The enzyme created 1.66 mg ml−1 Rg2 from Re with 553 mg l−1 h−1 , 0.85 mg ml−1 , and 1.01 mg ml−1 APPT from Rg1 and Rf with 283 and 316 mg l−1 h−1 APPT. Conclusions: β-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
12. Cloning and Characterization of β-Fructosidase from Thermotoga neapolitana DSM4359 and Through the Degraded Glucoside.
- Author
-
Bi, Yun-feng, Xu, Lin-lin, Chen, Ping, Jiang, Shan, and Shen, Ming-hao
- Published
- 2018
- Full Text
- View/download PDF
13. pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification.
- Author
-
Ming-Qi Liu, Wen-Feng Zeng, Pan Fang, Wei-Qian Cao, Chao Liu, Guo-Quan Yan, Yang Zhang, Chao Peng, Jian-Qiang Wu, Xiao-Jin Zhang, Hui-Jun Tu, Hao Chi, Rui-Xiang Sun, Yong Cao, Meng-Qiu Dong, Bi-Yun Jiang, Jiang-Ming Huang, Hua-Li Shen, Wong, Catherine C. L., and Si-Min He
- Abstract
The precise and large-scale identification of intact glycopeptides is a critical step in glycoproteomics. Owing to the complexity of glycosylation, the current overall throughput, data quality and accessibility of intact glycopeptide identification lack behind those in routine proteomic analyses. Here, we propose a workflow for the precise high-throughput identification of intact N-glycopeptides at the proteome scale using stepped-energy fragmentation and a dedicated search engine. pGlyco 2.0 conducts comprehensive quality control including false discovery rate evaluation at all three levels of matches to glycans, peptides and glycopeptides, improving the current level of accuracy of intact glycopeptide identification. The N-glycoproteome of samples metabolically labeled with
15 N/13 C were analyzed quantitatively and utilized to validate the glycopeptide identification, which could be used as a novel benchmark pipeline to compare different search engines. Finally, we report a large-scale glycoproteome dataset consisting of 10,009 distinct site-specific N-glycans on 1988 glycosylation sites from 955 glycoproteins in five mouse tissues. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
14. Pharmacogenomics of platinum-based chemotherapy in non-small cell lung cancer: focusing on DNA repair systems.
- Author
-
Xiong, Yi, Huang, Bi-Yun, and Yin, Ji-Ye
- Abstract
Drug therapy for non-small cell lung cancer consists mainly of platinum-based chemotherapy regimens. However, toxicity, drug resistance, and high risk of death have been seen in the clinic, which means there is a need for optimizing the use of medications. Platinum resistance could be mediated by a series of DNA repair pathways, and therefore, these pathways should be taken into account for optimizing drug using. The goal of pharmacogenomics is to elucidate genetic factors, such as DNA repair genes, which might underlie drug efficacy and effectiveness, and to improve therapeutic effects or guide personalized therapy as well. Here, we reviewed the current knowledge of pharmacogenomic data on DNA repair systems and examined whether they could be further translated into the clinic with evidence-based perspectives. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
15. Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system.
- Author
-
He, Yan, Zhou, Qiao-Hong, Liu, Bi-Yun, Cheng, Long, Tian, Yun, Zhang, Yong-Yuan, and Wu, Zhen-Bin
- Abstract
This investigation demonstrates that programmed cell death (PCD) in a cyanobacterium, Microcystis aeruginosa, resulting from allelopathic stress induced by a submerged macrophyte, Myriophyllum spicatum, in a co-culture system. The hallmarks of PCD, caspase-3-like protease activity, DNA fragmentation, and destruction of cell ultrastructure, as well as intracellular PCD signaling radicals, reactive oxygen species (ROS), and nitric oxide (NO), were measured in M. aeruginosa cells co-cultured with M. spicatum for 7 days. The results showed a dose-response relationship between M. spicatum biomass and M. aeruginosa mortality. A caspase-3-like protease was activated and elevated from day 3. Thylakoid disintegration, cytoplasmic vacuolation, and fuzzy nuclear zone were observed by transmission electron microscopy, and distinct DNA fragmentation was detected in M. aeruginosa cells at a M. spicatum biomass of 6.0 g fresh weight (FW) L during the 7 days. Allelochemicals of total phenolic compounds (TPCs) were determined in co-culture water, and the concentration increased with increasing of M. spicatum biomass and co-culture time. Compared with the level of ROS production in the control group, a significant overproduction of ROS was detected in M. aeruginosa cells in the treatment group, and this was positively correlated with TPC concentration. Furthermore, the level of intracellular NO increased with the percent mortality of M. aeruginosa. The results indicated that a PCD pathway was induced in the cyanobacterium M. aeruginosa when co-cultured with the submerged macrophyte M. spicatum. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
16. Ground-roll separation of seismic data based on morphological component analysis in two-dimensional domain.
- Author
-
Xu, Xiao-Hong, Qu, Guang-Zhong, Zhang, Yang, Bi, Yun-Yun, and Wang, Jin-Ju
- Subjects
SEISMIC testing ,SIGNAL-to-noise ratio ,DISCRETE wavelet transforms ,SEISMIC wave studies ,ALGORITHM research - Abstract
Ground roll is an interference wave that severely degrades the signal-to-noise ratio of seismic data and affects its subsequent processing and interpretation. In this study, according to differences in morphological characteristics between ground roll and reflected waves, we use morphological component analysis based on two-dimensional dictionaries to separate ground roll and reflected waves. Because ground roll is characterized by low-frequency, low-velocity, and dispersion, we select two-dimensional undecimated discrete wavelet transform as a sparse representation dictionary of ground roll. Because of a strong local correlation of the reflected wave, we select two-dimensional local discrete cosine transform as the sparse representation dictionary of reflected waves. A sparse representation model of seismic data is constructed based on a two-dimensional joint dictionary then a block coordinate relaxation algorithm is used to solve the model and decompose seismic record into reflected wave part and ground roll part.The good effects for the synthetic seismic data and application of real seismic data indicate that when using the model, strong-energy ground roll is considerably suppressed and the waveform of the reflected wave is effectively protected. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
17. The human chemokine receptor CCRL2 suppresses chemotaxis and invasion by blocking CCL2-induced phosphorylation of p38 MAPK in human breast cancer cells.
- Author
-
Wang, Lei-Ping, Cao, Jun, Zhang, Jian, Wang, Bi-Yun, Hu, Xi-Chun, Shao, Zhi-Min, Wang, Zhong-Hua, and Ou, Zhou-Luo
- Abstract
The human chemokine receptor CCRL2 is a member of the atypical chemokine receptor family. CCRL2 is unable to couple with G-proteins and fails to induce classical chemokine signaling for the highly conserved DRYLAIV motif essential for signaling has been changed to QRYLVFL. We investigated whether CCRL2 is involved in the chemotaxis, invasion, and proliferation of human breast cancer cells. Firstly, expression of CCRL2 was determined in six breast cancer cell lines by real-time RT-PCR and Western blot. Then, we established stable cell lines overexpressing CCRL2 to explore the function of CCRL2 in chemotaxis and invasion by transwell assays, and the signaling downstream was further investigated. The effect of CCRL2 on proliferation was detected by colony formation assays and tumor xenograft study. We found that stable overexpression of CCRL2 in MDA-MB-231 and BT-549 cells attenuated the chemotaxis and invasion stimulated by its ligand CCL2. CCRL2 inhibits p38 MAPK (p38) phosphorylation and up-regulates the expression of E-cadherin. This effect was eliminated by the inhibitor of p38 MAPK. CCRL2 inhibited the growth of breast cancer cells in vitro and in vivo. Our results suggest that CCRL2 functions as a tumor suppressor in human breast cancer cells. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
18. Combining Bone Marrow Stromal Cells with Green Tea Polyphenols Attenuates the Blood-Spinal Cord Barrier Permeability in Rats with Compression Spinal Cord Injury.
- Author
-
Yu, De-shui, Liu, Li-bo, Cao, Yang, Wang, Yan-song, Bi, Yun-long, Wei, Zi-jian, Tong, Song-ming, Lv, Gang, and Mei, Xi-fan
- Abstract
This study was performed to investigate the effect of bone marrow stromal cells (BMSCs) combined with green tea polyphenols (GTPs) on the blood-spinal cord barrier (BSCB) permeability after spinal cord injury (SCI) in the rat model. In the model of SCI rats, we found that the water content and the BSCB permeability were decreased by BMSCs and GTPs treatment, and their combination had a synergistic effect. Further, the motor function of rats was also greatly improved by BMSCs and GTPs administration. After treated by the combination of BMSCs and GTPs, SCI rats showed the up-regulated expression of tight junction (TJ) associated proteins claudin-5, occludin and ZO-1 by Western blot, which was more remarkable than that in the single treatment. The increased expression levels of claudin-5, occludin, and ZO-1 were the most obvious in the spinal cord microvessels using immunohistochemistry assay. This led to the conclusion that the combination of BMSCs and GTPs could decrease the BSCB permeability by up-regulating protein expression levels of claudin-5, occludin, and ZO-1. In addition, after BMSCs and GTPs administration, the results of Western blot and enzyme-linked immunosorbent assay (ELISA) revealed a significant decrease in protein expression level and the activation of nuclear factor-кB (NF-кB) p65. Our results indicated that combination of BMSCs and GTPs could improve motor function after SCI, which might be correlated with improvements in BSCB integrity, and that NF-кB might be involved in the modulating process. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
19. Clinical assessment and polysomnographic study of sleep apnea in a Chinese population of snorers.
- Author
-
Shao, Chuan, Jiang, Jing-bo, Wu, Hong-cheng, Wu, Shi-bo, Yu, Bi-yun, and Tang, Yao-dong
- Abstract
Copyright of Journal of Zhejiang University: Science B is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2015
- Full Text
- View/download PDF
20. Crystalline-coil diblock copolymers of syndiotactic polypropylene- b- poly(ethylene oxide): synthesis, solution self-assembly, and confined crystallization in nanosized micelle cores.
- Author
-
Liu, Ran, Li, Zhi-Yun, Mai, Bi-Yun, Wu, Qing, Liang, Guo-Dong, Gao, Hai-Yang, and Zhu, Fang-Ming
- Subjects
DIBLOCK copolymers ,POLYPROPYLENE ,POLYETHYLENE oxide ,MOLECULAR self-assembly ,SOLUTION (Chemistry) ,ORGANIC synthesis ,CRYSTALLIZATION ,NUCLEAR magnetic resonance spectroscopy ,POLYMERIZATION - Abstract
In this paper, two crystalline-coil diblock copolymers consisting of blocks of syndiotactic polypropylene (sPP) with different syndiotacticities ([rrrr] = 0.78, [rrrr] = 0.94) and poly(ethylene oxide) (PEO) were synthesized utilizing the click reaction of azide-terminated PEO (PEO-N) with alkynyl-terminated sPP (sPP-C≡CH), which was obtained via the syndiospecific polymerization of propylene using fluorinated bis(phenoxyimine) titanium catalysts and further end-group transformation. All products and intermediates were characterized by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). We probed the temperature-induced self-assembly of sPP- b-PEO in the solvent N, N′-dimethylformamide (DMF), which is selective for PEO. The self-assembly behavior and micellar morphology of each sPP- b-PEO were investigated by dynamic light scattering (DLS), transmission electron microscopy (TEM), and atomic force microscopy (AFM). Before sPP crystallization, the crystalline-coil diblock copolymers self-assembled into spherical micelles with an amorphous sPP core and a soluble PEO corona. Platelet-like micelles with a crystallized sPP core sandwiched between two solvent-swollen PEO layers then formed upon the crystallization of the sPP block. Furthermore, the confined crystallization of sPP within nanosized micelle cores was investigated by differential scanning calorimetry (DSC). An enormous degree of supercooling was observed during crystallization due to the homogeneous nucleation. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
21. Vinorelbine and capecitabine in anthracycline- and/or taxane-pretreated metastatic breast cancer: sequential or combinational?
- Author
-
Zhang, Jian, Gu, Shi-Yang, Gan, Yu, Wang, Zhong-Hua, Wang, Bi-Yun, Guo, Hai-Yi, Wang, Jia-Lei, Wang, Lei-Ping, Zhao, Xin-Min, and Hu, Xi-Chun
- Subjects
BREAST cancer treatment ,VINORELBINE ,ANTHRACYCLINES ,DRUG interactions ,DRUG administration ,THYMIDYLATE synthase ,GENE expression - Abstract
Purpose: The difference between combinational and pre-planned sequential therapies using regimens that include non-anthracycline and taxane in the first-line setting remains unclear. The purpose of this study is to explore the interaction between vinorelbine (N) and capecitabine (X) in breast cancer cells and to compare the simultaneous or sequential administration of the two drugs in patients with metastatic breast cancer (MBC) as first-line treatment. Methods: First, we explored the effects of vinorelbine on thymidine phosphorylase (TP) and thymidylate synthase (TS) expression in breast cancer cells. Next, we designed a prospective randomized phase II trial of MBC patients comparing the combinational and pre-planned sequential administration of vinorelbine and capecitabine in the first-line metastatic setting. The primary end point was progression-free survival (PFS). The correlation between clinical characteristics and class III β-tubulin expression and patient survival was also explored. Results: Vinorelbine upregulates TP and downregulates TS in breast cancer cells, thereby further sensitizing tumor cells to capecitabine, which indicated the proper order for sequential therapy should be N → X. Sixty patients were eligible for the phase II trial. No significant difference was observed between the combinational arm and the sequential arm in terms of progression-free survival (PFS), overall response rate (ORR), and overall survival (OS). Only in the subgroup of patients with liver metastases were median PFS and OS significantly prolonged in the combinational arm (8.5 vs. 6.4 months, P = 0.041 and 23.8 vs. 13.9 months, P = 0.028, respectively). No association between class III β-tubulin expression and patient outcome was identified. Grade 3/4 adverse events were more common in the combinational arm. Conclusions: Both the NX regimen and pre-planned sequential N → X regimen are acceptable as first-line treatments with comparable efficacies for MBC patients previously treated with anthracyclines and/or taxanes. Sequential monotherapies are recommended as the preferred approach to first-line chemotherapy for most MBC patients in the absence of an imminent visceral crisis and the need for rapid symptom and/or disease control. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
22. Spinal meningioma in childhood: clinical features and treatment.
- Author
-
Wang, Xiao-Qiang, Zeng, Xian-Wei, Zhang, Bi-Yun, Dou, Ya-Fang, Wu, Jin-Song, Jiang, Cheng-Chuan, Zhong, Ping, and Chen, Hong
- Subjects
MENINGIOMA ,CENTRAL nervous system tumors ,NEUROFIBROMATOSIS in children ,THORACIC vertebrae ,THERAPEUTICS - Abstract
Object: The purpose of this retrospective study is to determine the clinical characteristics and the prognosis of the spinal meningioma in childhood (under 18 years of age) based on the treatment at our institution. Methods: Ten spinal meningioma cases in children were treated during the last 9 years. The clinical data was retrospectively analyzed and the results were compared with those in the literature. Results: The series included eight males and two females and the mean age was 13.2 years. The most common initial symptoms were pain (6/10) and the common signs were limb weakness (4/10) and gait disturbance (2/10) and distal paresthesia (1/10) and bladder disturbance (1/10). Four patients had other clinical signs of neurofibromatosis type II (NF-2) such as tumors elsewhere. All the tumors were located in cervical and thoracic vertebrae. Resection according to Simpson Grade I (6/10), II (2/10), III (1/10), and IV (1/10) were performed. Grade II meningiomas accounted for 3/10 in this series. All patients were followed up with mean follow-up period of 43 months. Seven patients had recurrence of the tumor in that period and one had died. Conclusions: Spinal meningioma is an uncommon pediatric neoplasm and has a poor prognosis. It has a male predominance and is inclined to be associated with NF-2, and those that are associated with higher pathologic subtypes and NF-2 have more unfavorable outcome. Every effort should be made to achieve total removal which may decrease the incidence of recurrence. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
23. Simulation of the effect of water-vapor increase on temperature in the stratosphere.
- Author
-
BI Yun, CHEN Yuejuan, ZHOU Renjun, YI Mingjian, and DENG Shumei
- Subjects
- *
ATMOSPHERIC water vapor , *ATMOSPHERIC temperature , *STRATOSPHERE , *COMPUTER simulation , *HEAT radiation & absorption - Abstract
To analyze the mechanism by which water vapor increase leads to cooling in the stratosphere, the effects of water-vapor increases on temperature in the stratosphere were simulated using the two-dimensional, interactive chemical dynamical radiative model (SOCRATES) of NCAR. The results indicate that increases in stratospheric water vapor lead to stratospheric cooling, with the extent of cooling increasing with height, and that cooling in the middle stratosphere is stronger in Arctic regions. Analysis of the radiation process showed that infrared radiative cooling by water vapor is a pivotal factor in middle-lower stratospheric cooling. However, in the upper stratosphere (above 45 km), infrared radiation is not a factor in cooling; there, cooling is caused by the decreased solar radiative heating rate resulting from ozone decrease due to increased stratospheric water vapor. Dynamical cooling is important in the middle-upper stratosphere, and dynamical feedback to temperature change is more distinct in the Northern Hemisphere middle-high latitudes than in other regions and significantly affects temperature and ozone in winter over Arctic regions. Increasing stratospheric water vapor will strengthen ozone depletion through the chemical process. However, ozone will increase in the middle stratosphere. The change in ozone due to increasing water vapor has an important effect on the stratospheric temperature change. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
24. Transient characteristics of residual meridional circulation during stratospheric sudden warming.
- Author
-
Deng, Shumei, Chen, Yuejuan, Huang, Yong, Luo, Tao, and Bi, Yun
- Abstract
The residual meridional circulation derived from the transformed Eulerian-mean thermodynamic equation and continuity equation can be separated into two parts, the slowly varying diabatic circulation and the transient circulation, as demonstrated by others. We calculated and composite-analyzed the transient and diabatic circulation for 14 stratospheric sudden warming (SSW) events from 1979-2002 by using the daily ECMWF reanalysis data. Specifically, the transient residual meridional circulation was calculated both with and without inclusion of the eddy heat transport term in the transformed Eulerian-mean thermodynamic equation to investigate the importance of the eddy heat transport term. The results showed that calculations of transient residual meridional circulation present rapid variations during SSWs, with or without inclusion of the eddy heat transport term. Although the patterns of transient residual meridional circulation with the eddy heat transport term were similar to that without the eddy heat transport term during SSW, the magnitudes in the upper stratosphere and high-latitude regions differed. As for the diabatic circulation, its daily variations were small during SSW events, and its patterns were in agreement with its monthly average. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
25. Lack of association between methylenetetrahydrofolate reductase gene A1298C polymorphism and breast cancer susceptibility.
- Author
-
Li-Xin Qiu, Jian Zhang, Wen-Hua Li, Qun-Ling Zhang, Hui Yu, Bi-Yun Wang, Lei-Ping Wang, Jia-Lei Wang, Hui-Jie Wang, Xiao-Jian Liu, Zhi-Guo Luo, and Xiang-Hua Wu
- Abstract
Published data on the association between methylenetetrahydrofolate reductase gene (MTHFR) A1298C polymorphism and breast cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Medline, PubMed, Embase, and Web of Science were searched. Crude ORs with 95% CIs were used to assess the strength of association between the MTHFR A1298C polymorphism and breast cancer risk. The pooled ORs were performed for co-dominant model (AC vs. AA, CC vs. AA), dominant model (CC + AC vs. AA), and recessive model (CC vs. AC + AA), respectively. A total of 26 studies including 12,244 cases and 15,873 controls were involved in this meta-analysis. Overall, no significant associations were found between MTHFR A1298C polymorphism and breast cancer risk when all studies pooled into the meta-analysis (AC vs. AA: OR = 0.99, 95% CI 0.94-1.05; CC vs. AA: OR 0.99, 95% CI 0.90-1.09; dominant model: OR = 0.99, 95% CI 0.95-1.04; and recessive model: OR = 0.98, 95% CI 0.90-1.08). In the subgroup analysis by ethnicity or study design, still no significant associations were found for all comparison models. In conclusion, this meta-analysis suggests that the MTHFR A1298C polymorphism may be not associated with breast cancer development. However, large sample and representative population-based studies with homogeneous breast cancer patients and well matched controls are warranted to confirm this finding. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
26. Current evidence on the relationship between three polymorphisms in the FGFR2 gene and breast cancer risk: a meta-analysis.
- Author
-
Zhang, Jian, Qiu, Li-Xin, Wang, Zhong-Hua, Leaw, Shiang-Jiin, Wang, Bi-Yun, Wang, Jia-Lei, Cao, Zhi-Gang, Gao, Jia-Li, and Hu, Xi-Chun
- Abstract
In this article, inconsistency of the association of polymorphisms of fibroblast growth factor receptor 2 (FGFR2) with breast cancer is noted. Three commonly studied FGFR2 polymorphisms including rs1219648 (A > G), rs2420946 (C > T), and rs2981582 (C > T) were selected to explore their association with risk of development of breast cancer by meta-analysis of published case-control studies. The results showed that all these three polymorphisms were significantly associated with altered breast cancer risk in any model (co-dominant, dominant, or recessive model) and in stratification based on ethnicity and study design. In the subgroup analyses for postmenopausal women, significantly increased risks were found for rs1219648 and rs2420946 in any model. This meta-analysis suggests that FGFR2 is likely an important genetic marker contributing to susceptibility of breast cancer. We recommend that these single nucleotide polymorphisms to be included in future association studies and functional assays. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
27. Simulation of the effect of an increase in methane on air temperature.
- Author
-
Bi, Yun, Chen, Yuejuan, Zhou, Renjun, Yi, Mingjian, and Deng, Shumei
- Abstract
The infrared radiative effect of methane was analyzed using the 2D, interactive chemical dynamical radiative SOCRATES model of the National Center for Atmospheric Research. Then, a sensitivity experiment, with the methane volume mixing ratio increased by 10%, was carried out to study the influence of an increase of methane on air temperature. The results showed that methane has a heating effect through the infrared radiative process in the troposphere and a cooling effect in the stratosphere. However, the cooling effect of the methane is much smaller than that of water vapor in the stratosphere and is negligible in the mesosphere. The simulation results also showed that when methane concentration is increased by 10%, the air temperature lowers in the stratosphere and mesosphere and increases in the troposphere. The cooling can reach 0.2 K at the stratopause and can vary from 0.2-0.4 K in the mesosphere, and the temperature rise varies by around 0.001-0.002 K in the troposphere. The cooling results from the increase of the infrared radiative cooling rate caused by increased water vapor and O concentration, which are stimulated by the increase in methane in most of the stratosphere. The infrared radiation cooling of methane itself is minor. The depletion of O stimulated by the methane increase results indirectly in a decrease in the rate of solar radiation heating, producing cooling in the stratopause and mesosphere. The tropospheric warming is mainly caused by the increase of methane, which produces infrared radiative heating. The increase in HO and O caused by the methane increase also contributes to a rise in temperature in the troposphere. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
28. MTHFR C677T polymorphism associated with breast cancer susceptibility: a meta-analysis involving 15,260 cases and 20,411 controls.
- Author
-
Zhang, Jian, Qiu, Li-Xin, Wang, Zhong-Hua, Wu, Xiang-Hua, Liu, Xiao-Jian, Wang, Bi-Yun, and Hu, Xi-Chun
- Abstract
Published data on the association between MTHFR C677T polymorphism and breast cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Medline, PubMed, Embase, and Web of Science were searched. Crude ORs with 95% CIs were used to assess the strength of association between the MTHFR C677T polymorphism and breast cancer risk. The pooled ORs were performed with co-dominant model (CT vs. CC, TT vs. CC), dominant model (CT + TT vs. CC), and recessive model (TT vs. CC + CT), respectively. A total of 37 studies including 15,260 cases and 20,411 controls were involved in this meta-analysis. Overall, significantly elevated breast cancer risk was associated with TT variant genotype in homozygote comparison and dominant genetic model when all studies were pooled into the meta-analysis (TT vs. CC: OR = 1.11, 95% CI = 1.01–1.23; dominant model: OR = 1.04, 95% CI = 1.00–1.09). In the subgroup analysis by ethnicity, significantly increased risks were found for TT allele carriers among Asians (TT vs. CC: OR = 1.18, 95% CI = 1.04–1.35; recessive model: OR = 1.15, 95% CI = 1.03–1.29). When stratified by study design, statistically significantly elevated risk was found in hospital-based studies (TT vs. CC: OR = 1.18, 95% CI = 1.02–1.38; recessive model: OR = 1.17, 95% CI = 1.05–1.29). In the subgroup analysis by menopausal status, statistically significantly increased risk was found among postmenopausal women (CT vs. CC: OR = 1.12, 95% CI = 1.02–1.23; dominant model: OR = 1.11, 95% CI = 1.01–1.22). In conclusion, this meta-analysis suggests that the MTHFR T allele is a low-penetrant risk factor for developing breast cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
29. Peripheral cholinoceptor antagonist anisodamine counteracts cholinergic adverse effects and facilitates cognitive amelioration of rivastigmine.
- Author
-
Wei-Wei Zhang, Zu-Peng Xu, Yong-Yao Cui, Hao Wang, Ming-Ke Song, Juan Li, Bi-Yun Shao, Zheng Xia, and Hong-Zhuan Chen
- Subjects
CHOLINERGIC receptors ,ALZHEIMER'S disease ,CHOLINESTERASE inhibitors ,ENZYME inhibitors ,THERAPEUTICS - Abstract
Rivastigmine is a potent acetyl- and butyrylcholinesterase inhibitor widely used for cognitive improvement in Alzheimer’s disease (AD) therapy. However, dose-limiting adverse effects restrict its tolerability and clinical outcomes. This study explored new combined therapy, in which peripheral cholinergic adverse effects and central cognitive amelioration of rivastigmine were differentiated by a peripheral cholinoceptor antagonist anisodamine. The results demonstrated that rivastigmine (0.75 and 2.0 mg/kg) could significantly reverse the scopolamine-induced cognitive deficit in mice through passive avoidance test. Nevertheless, a high dose of rivastigmine (3.25 mg/kg) would compromise cognitive amelioration and produce obvious adverse effects, including hypersalivation, intestinal hyperperistalsis and muscle cramp. Interestingly, concomitant administration of anisodamine (10 mg/kg) effectively counteracted both the muscarinergic and nicotinergic adverse effects, while facilitating cognitive amelioration of rivastigmine (3.25 mg/kg). These findings provide an insight into the feasibility of combined therapy with cholinesterase inhibitors and peripheral cholinoceptor antagonists for the treatment of AD. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
30. Genetic diversity in oil and vegetable mustard (Brassicajuncea) landraces revealed by SRAP markers.
- Author
-
Bi-yun Chen, Guangyuan Lu, Han-zhong Wang, Kun Xu, Gao Guizhan, and Yunchun Song
- Abstract
Abstract Mustard (Brassica juncea) is an important crop in both ancient and modern world. It has a broad resource of genetic diversity that is used primarily as oilseed but as vegetables, condiment and medicines also. Its superior tolerance to adverse environments, e.g., drought, high temperature and low fertility suggests its better adaptability in future possible harsh environments. Chinese vegetable mustard displays a wide spectrum of morphotypes. A collection of 95 accessions of B. juncea representing oil and vegetable mustards from China, France, India, Pakistan, and Japan were assessed to determine diversity at the molecular level using sequence-related amplified polymorphism (SRAP). Eight SRAP primer combinations identified a total of 326 scorable fragments of which 161 were polymorphic (49.39%). The percentage of polymorphism for each primer combination varied from 21.88 to 66.67%. Both Shannon-Weaver and Simpson genetic diversity index indicated that the level of genetic diversity within vegetable mustard is much higher than within oil mustard, and also winter oil mustards are genetically more diverse than spring oil mustards. Based on the Cluster and Principal Coordinates analysis, which were conducted on the similarity matrix of SRAP marker data, vegetable, spring oil and winter oil mustard were clearly divided into three distinct groups and among these three groups, spring and winter oil mustard are geneticlly closer than vegetable mustard. This suggests that bilateral gene exchange between oil and vegetable gene pools in the breeding program will effectively elevate the genetic potential in developing higher yields, more disease resistance, better quality and better adapted lines. [ABSTRACT FROM AUTHOR]
- Published
- 2009
31. Machining distortion prediction of aerospace monolithic components.
- Author
-
Bi, Yun-bo, Cheng, Qun-lin, Dong, Hui-yue, and Ke, Ying-lin
- Abstract
To predict the distortion of aerospace monolithic components, a model is established to simulate the numerical control (NC) milling process using 3D finite element method (FEM). In this model, the cutting layer is simplified firstly. Then, the models of cutting force and cutting temperature are established to gain the cutting loads, which are applied to the mesh model of the part. Finally, a prototype of machining simulation environment is developed to simulate the milling process of a spar. Key factors influencing the distortion, such as initial residual stress, cutting loads, fixture layout, cutting sequence, and tool path are considered all together. The total distortion of the spar is predicted and an experiment is conducted to validate the numerical results. It is found that the maximum discrepancy between the simulation results and experiment values is 19.0% [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
32. The possible influence of stratospheric sudden warming on East Asian weather.
- Author
-
Deng, Shumei, Chen, Yuejuan, Luo, Tao, Bi, Yun, and Zhou, Houfu
- Abstract
By analyzing the linkage of the Northern Annular Mode (NAM) anomaly to the East Asian jet and the East Asian trough during Stratospheric Sudden Warming (SSW), the influence of SSW on East Asian weather is studied. The results show that the East Asian jet is strengthened and the East Asian trough is deepened during SSW. With the downward propagation of SSW, the strengthened East Asian jet and the East Asian trough would move southward, expand westward and gradually influence the area of north and northeastern China. This implies that the winter monsoon tends to be enhanced over East Asia during SSW. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
33. Electrochemical immune bioassay for the antigen–antibody interaction based on [Fe(CN)6]4-/3- and [AuCl4]- ions-derivated biomimetic interface.
- Author
-
Tang, Dian-Yong and Xia, Bi-Yun
- Abstract
A simple and sensitive electrochemical immune bioassay for the detection of hepatitis B surface antigen (HBsAg), as a model, was developed based on [Fe(CN)
6 ]4-/3- and [AuCl4 ]- ions-derivated biomimetic interface in this study. A layer of [Fe(CN)6 ]4-/3- film (i.e., Prussian blue, PB) was initially electrodeposited onto a glassy carbon electrode, and then [AuCl4 ]- ions were reduced under the potentiostat to form gold nanoparticles on the PB film. Finally, hepatitis B surface antibody (HBsAb) was adsorbed onto the nanogold surface. The performance and factors influencing the immunosensor were assessed and optimized. The proposed immunosensor exhibits a specific response to HBsAg in the range of 2.13–314.3 ng∙ml-1 with a detection limit of 0.42 ng∙ml-1 . In addition, the developed immunosensor shows high sensitivity, good reproducibility, and long-term stability. Importantly, the ions-derivated biomimetic interface could be further extended for the immobilization of other proteins and biocompound. [ABSTRACT FROM AUTHOR]- Published
- 2008
- Full Text
- View/download PDF
34. Effect of Pyrogallol on the Growth and Pigment Content of Cyanobacteria-Blooming Toxic and Nontoxic Microcystis Aeruginosa.
- Author
-
Liu, Bi-Yun, Zhou, Pei-Jiang, Tian, Jian-Ru, and Jiang, Shun-Yao
- Subjects
ALGAL blooms ,MICROCYSTIS aeruginosa ,CYANOBACTERIA ,PHENOLS & the environment ,PHYTOPLANKTON ,AQUATIC resources ,SPECTRUM analysis ,MICROALGAE - Abstract
This article discusses the control of algal blooms through the use of pyrogallol produced by tissues in Myriophyllum spicatum. According to the authors, pyrogallol produces an inhibitory algicidal effect on both toxic and non-toxic Microcystis aeruginosa. There was a clear dose-response relationship with pyrogallol and a decrease in pigment absorption suggesting a decrease in the collection of solar energy. They concluded that the rate of inhibition was strongly dependent on the concentration of pyrogallol and the pH. At higher concentrations and higher pH the rate of inhibition of toxic M. aeruginosa were stronger.
- Published
- 2007
- Full Text
- View/download PDF
35. Study on the trace species in the stratosphere and their impact on climate.
- Author
-
Chen, Yuejuan, Zhou, Renjun, Shi, Chunhua, and Bi, Yun
- Abstract
The trace gases (O
3 , HCl, CH4 , H2 O, NO, NO2 ) in the stratosphere play an important role, not only in the photochemical processes in which the ozone layer destroyed, but also in the radiative processes. In this paper, we review the works on the distribution and variation of the trace gases in the stratosphere and their impact on climate, which have been carried out at the University of Science and Technology of China in the recent 20 years. The Halogen Occultation Experiment (HALOE) data were used to analyse the distribution and variation of the mixing ratio of these trace gases and the temperature trends in the stratosphere in the most recent decade. And the reanalyzed National Centers of Environmental Prediction (NCEP)/NCAR data were also used to give the temperature trends and compared with the results from HALOE data. Numerical simulations were also carried out to study the impact of ozone depletion on the global climate. In this review, the distributions of the trace gases, especially those over the Qinghai-Xizang Plateau, are discussed, and the variations and trends for the trace gases in various levels in the stratosphere have been given for the most recent decade. The temperature variation and the cooling trend obtained from HALOE data in the middle and lower stratosphere for the last 13 years are significant, which agree well with the results from NCEP/NCAR data. While the temperature trend in the upper stratosphere in this period do not seem to have much cooling. The numerical simulations show that either the Antarctic ozone hole or the ozone valley over Qinghai-Xizang Plateau affect not only the temperature and circulation in the stratosphere, but also the temperature, pressure and wind fields in the troposphere, then lead to the global climate change. [ABSTRACT FROM AUTHOR]- Published
- 2006
- Full Text
- View/download PDF
36. Natural abundance heteronuclear NMR studies of the T3 mini-loop hairpin in the terminal repeat of the adenoassociated virus 2.
- Author
-
Shan-Ho Chou, Yu-Yu Tseng, and Bi-Yun Chu
- Subjects
MAGNETIC resonance imaging ,VIRUSES ,GENETIC engineering ,DNA ,GENE therapy ,COUPLING constants - Abstract
A DNA hairpin containing a T
3 loop, as occurs in the terminal repeat of a popular gene therapy vector (Adenoassociated Virus 2, AAV2), has been extensively studied using homo- and heteronuclear NMR experiments. Almost complete assignment of the proton and carbon resonances, including H5′(Pro-S) and H5′(Pro-R) protons, has been accomplished at natural abundance. NOESY spectra in H2 O and D2 O have revealed many unusual NOEs, which, when combined with the ε, β, γ, and χ torsion angles determined from heteronuclear1 H-13 C,1 H-31 P, and13 C-31 P coupling constants, have allowed for a more detailed picture of the T3 mini-loop hairpin. The three loop thymidines are all unpaired, yet are highly structured when bracketed by a 5′-GC...GC-3′ stem sequence. The structure determined in this manuscript is considerably different from several other structures reported so far. Contrary to an RNA oligomer with a central U3 sequence that has the tendency to form a duplex with three U· U mismatches, the d(GAAGC-TTT-GCTTC) sequence exists mostly as a hairpin under millimolar NMR conditions. Since T3 triloop was found to be an essential element for the site-specific non-homologous integration of the AAV2 virus, and modification of the T3 loop residue abolishes such capability, the structure we report here may be of biological significance. [ABSTRACT FROM AUTHOR]- Published
- 2000
- Full Text
- View/download PDF
37. Metabolites from the fungus Cephalosporium sp. AL031.
- Author
-
Bi, Yun-Mei, Bi, Xu-Bin, A, Fang, and Zhao, Qian-Rong
- Abstract
A new pyrone derivative, 7, 9-dihydroxy-10-methyl-2H, 4aH, 6H, 10bH-pyrano[5,6-c][2]ben-zopyran-2, 6-dione ( 1), was isolated from a culture broth of a strain of the fungus Cephalosporium sp. AL031, together with three known compounds, 3-acetyl-7-hydroxy-5-methoxyl-3 H-isobenzofuran-1-one (2), vermopyrone (3), and 5-methylmellein ( 4). Their structures were elucidated by spectroscopic analysis including MS and 2D-NMR. Compounds 2, 3, and 4 are reported for the first time from fermentation broth of this fungus through the present study. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
38. Pristimerin induces apoptosis and autophagy via activation of ROS/ASK1/JNK pathway in human breast cancer in vitro and in vivo.
- Author
-
Zhao, Qun, Liu, Yingxiang, Zhong, Jing, Bi, Yun, Liu, Yongqiang, Ren, Ziting, Li, Xiang, Jia, Junjun, Yu, Mengting, and Yu, Xianjun
- Published
- 2019
- Full Text
- View/download PDF
39. Identification and profiling of microRNAs during gonadal development in the giant freshwater prawn Macrobrachium rosenbergii.
- Author
-
Liu, Xue, Luo, Bi-Yun, Feng, Jian-Bin, Zhou, Ling-Xia, Ma, Ke-Yi, and Qiu, Gao-Feng
- Abstract
As post-transcriptional regulators, microRNAs (miRNAs) play an important role in growth and reproductive processes. So far, there is limited information regarding crustacean miRNAs. To explore the potential role of miRNAs in the gonadal development of the prawn Macrobrachium rosenbergii, we constructed seven small RNA libraries from ovarian and testicular tissues at various stages using somatic tissue as the control. A total of 1,954 known and 129 novel miRNAs were retrieved. By comparing differentially expressed miRNAs (DEMs) between testes and ovaries, forty-one miRNAs were identified with sex-biased expression patterns, including 17 ovary-biased and 24 testis-biased patterns. Furthermore, the putative target genes of the sex-biased miRNAs, such as cyclin L1, mitogen-activated protein kinase 7 (MAPK 7), heat shock protein (HSP), and zinc finger protein, were significantly enriched in many reproduction-related pathways including the Gonadotropin-releasing hormone (GnRH) pathway, glycolysis, gluconeogenesis pathway, ovarian steroidogenesis, estrogen signaling pathway, MAPK pathway, Wnt pathway, and insulin signaling pathway, implicating potential regulatory roles of miRNAs in reproduction. These data aid in the further investigation of the mechanism of gonadal development and reproductive regulation mediated by miRNA in M. rosenbergii. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
40. Erratum to: Intermittent high dose proton pump inhibitor enhances the antitumor effects of chemotherapy in metastatic breast cancer
- Author
-
Xi Chun Hu, Lei Ping Wang, Zhong Hua Wang, Jia Lei Wang, En Ying Cao, Stefano Fais, Si Sun, Zhi Min Shao, Jian Zhang, Fang Fang Lv, Qun Ling Zhang, and Bi Yun Wang
- Subjects
Oncology ,Adult ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,medicine.drug_class ,medicine.medical_treatment ,Proton-pump inhibitor ,Breast Neoplasms ,Pilot Projects ,Drug Administration Schedule ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Medicine ,Humans ,Aged ,Chemotherapy ,business.industry ,Research ,Esomeprazole ,Proton Pump Inhibitors ,Middle Aged ,medicine.disease ,Metastatic breast cancer ,Treatment Outcome ,Cancer research ,Female ,business - Abstract
Acidity is a hallmark of malignant tumor, representing a very efficient mechanism of chemoresistance. Proton pump inhibitors (PPI) at high dosage have been shown to sensitize chemoresistant human tumor cells and tumors to cytotoxic molecules. The aim of this pilot study was to investigate the efficacy of PPI in improving the clinical outcome of docetaxel + cisplatin regimen in patients with metastatic breast cancer (MBC).Patients enrolled were randomly assigned to three arms: Arm A, docetaxel 75 mg/m(2) followed by cisplatin 75 mg/m(2) on d4, repeated every 21 days with a maximum of 6 cycles; Arm B, the same chemotherapy preceded by three days esomeprazole (ESOM) 80 mg p.o. bid, beginning on d1 repeated weekly. Weekly intermittent administration of ESOM (3 days on 4 days off) was maintained up to maximum 66 weeks; Arm C, the same as Arm B with the only difference being dose of ESOM at 100 mg p.o. bid. The primary endpoint was response rate.Ninety-four patients were randomly assigned and underwent at least one injection of chemotherapy. Response rates for arm A, B and C were 46.9, 71.0, and 64.5 %, respectively. Median TTP for arm A (n = 32), B (n = 31), C (n = 31) were 8.7, 9.4, and 9.7 months, respectively. A significant difference was observed between patients who had taken PPI and who not with ORR (67.7 % vs. 46.9 %, p = 0.049) and median TTP (9.7 months vs. 8.7 months, p = 0.045) [corrected]. Exploratory analysis showed that among 15 patients with triple negative breast cancer (TNBC), this difference was bigger with median TTP of 10.7 and 5.8 months, respectively (p = 0.011). PPI combination showed a marked effect on OS as well, while with a borderline significance (29.9 vs. 19.2 months, p = 0.090). No additional toxicity was observed with PPI.The results of this pilot clinical trial showed that intermittent high dose PPI enhance the antitumor effects of chemotherapy in MBC patients without evidence of additional toxicity, which requires urgent validation in a multicenter, randomized, phase III trial.Clinicaltrials.gov identifier: NCT01069081 .
- Full Text
- View/download PDF
41. The maximum standardized uptake value of 18 F-FDG PET scan to determine prognosis of hormone-receptor positive metastatic breast cancer
- Author
-
Yong Ping Zhang, Min Zhou, Zhong Hua Wang, Jian Zhang, Xi Chun Hu, Joseph Ragaz, Gang Li, Ying Jian Zhang, Bi Yun Wang, and Zhen Jia
- Subjects
Oncology ,Adult ,medicine.medical_specialty ,Cancer Research ,PET/CT ,Luminal subtype ,Standardized uptake value ,Breast Neoplasms ,lcsh:RC254-282 ,Disease-Free Survival ,Breast cancer ,Surgical oncology ,Fluorodeoxyglucose F18 ,Predictive Value of Tests ,Internal medicine ,medicine ,Genetics ,Humans ,Prospective Studies ,Aged ,Proportional Hazards Models ,PET-CT ,Univariate analysis ,business.industry ,SUVmax ,Middle Aged ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Metastatic breast cancer ,Prognosis ,Primary tumor ,Receptors, Estrogen ,Predictive value of tests ,Lymphatic Metastasis ,Positron-Emission Tomography ,Female ,Radiopharmaceuticals ,business ,Receptors, Progesterone ,Research Article - Abstract
Background Whether PET scan maximum standard uptake value (SUVmax) could differentiate luminal A from luminal B and help predict the survival of metastatic breast cancer (MBC) patients with luminal subtype is still unknown and need to be investigated. Methods 305 MBC patients with luminal subtypes were screened with PET/CT. Eligible patients were prospectively followed up. Results In total, 134 patients were eligible for this study. SUVmax was significantly related to the number of metastatic sites and presence of visceral metastasis on univariate analysis. SUVmax could not effectively differentiate patients with luminal A from luminal B subtype. Although luminal subtype at diagnosis could predict the relapse-free interval, it could not predict progression-free survival (PFS) or overall survival (OS) after developing relapse. In contrast, SUVmax was predictive of both PFS and OS and this effect was maintained in multivariate COX regression model. Conclusions SUVmax of MBC did not correlate with molecular subtypes of primary tumor. While molecular subtype may be a valuable prognostic factor at primary diagnosis of breast cancer, the SUVmax, rather than molecular subtype, does have a potential to predict independently in multivariate analysis for the PFS and OS in patients with metastatic disease of luminal subtype.
- Full Text
- View/download PDF
42. Excitation-dependent visible fluorescence in decameric nanoparticles with monoacylglycerol cluster chromophores.
- Author
-
Lee, Kwang-Ming, Cheng, Wan-Yin, Chen, Cheng-Yu, Shyue, Jing-Jong, Nieh, Chih-Chun, Chou, Chen-Fu, Lee, Jia-Rong, Lee, Ya-Yun, Cheng, Chih-Yang, Chang, Sarah Y., Yang, Thomas C., Cheng, Mei-Ching, and Lin, Bi-Yun
- Abstract
Organic fluorescent nanoparticles, excitation-dependent photoluminescence, hydrogen-bonded clusters and lysobisphosphatidic acid are four interesting individual topics in materials and biological sciences. They have attracted much attention not only because of their unique properties and important applications, but also because the nature of their intriguing phenomena remained unclear. Here we report a new type of organic fluorescent nanoparticles with intense blue and excitation-dependent visible fluorescence in the range of 410-620?nm. The nanoparticles are composed of ten bis(monoacylglycerol)bisphenol-A molecules and the self-assembly occurs only in elevated concentrations of 2-monoacylglycerol via radical-catalysed 3,2-acyl migration from 3-monoacylglycerol in neat conditions. The excitation-dependent fluorescence behaviour is caused by chromophores composed of hydrogen-bonded monoacylglycerol clusters, which are linked by an extensive hydrogen-bonding network between the ester carbonyl groups and the protons of the alcohols with collective proton motion and HO···C=O (n??*) interactions. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.