1. Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique.
- Author
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Sanyasi, Chandrasekar, Balakrishnan, Susmida Seni, Chinnasamy, Thirunavukkarasu, Venugopalan, Nagarajan, Kandavelu, Palani, Batra-Safferling, Renu, and Muthuvel, Suresh Kumar
- Subjects
PROTEIN disulfide isomerase ,SMALL-angle scattering ,SMALL-angle X-ray scattering - Abstract
The dynamic behavior of Protein Disulfide Isomerase (PDI) in an aqueous solution environment under physiologically active pH has been experimentally verified in this study using Small Angle X-ray Scattering (SAXS) technique. The structural mechanism of dimerization for full-length PDI molecules and co-complex with two renowned substrates has been comprehensively discussed. The structure models obtained from the SAXS data of PDI purified from bovine liver display behavior duality between unaccompanied-enzyme and after engaged with substrates. The analysis of SAXS data revealed that PDI exists as a homo-dimer in the solution environment, and substrate induction provoked its segregation into monomer to enable the enzyme to interact systematically with incoming clients. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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