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Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII). Those infections, usually hematogenous, mostly originate from the urinary tract. We investigated the strategies developed by E. coli in this context to evade host innate immune responses, i.e. complement and polymorphonuclear neutrophils (PMN). Twenty strains from OII were compared with 20 strains from bacteremia in patients with non-infected orthopedic implant. In both groups, 6/20 (30 %) strains lysed PMNs, due to the production of the pore-forming toxin α-hemolysin (HlyA). For the others, resistance to phagocytic killing by PMN was not significantly different between both groups. In contrast, resistance to complement-mediated serum killing was significantly higher in OII strains than in the others (65 % vs 10 %; P <0.001). In E. coli, different mechanisms have been involved in complement resistance. Here, serum resistance was not linked to a group 2 capsule, or a loss of outer membrane permeability, or the recruitment of the complement inhibitor C4bp, but was significantly associated with the synthesis of long-chain LPS, regardless of the O-antigen. Thus, serum resistance could promote seeding of peri-implant tissues by helping E. coli to either persist in blood and reach the site of infection or overcome localized complement activation. [ABSTRACT FROM AUTHOR]

The prospective cohort study presented here assessed the risk factors associated with Pseudomonas aeruginosa gastrointestinal colonization (PAGIC) in 933 patients hospitalized in five different wards in a French university hospital. A total of 195 patients were colonized. By logistic regression, hospitalization in an intensive care unit and length of hospital stay were independent risk factors. A significant association was observed between fluoroquinolone use and PAGIC caused by an ofloxacin-resistant strain ( p<0.0001), imipenem use and PAGIC caused by an imipenem-resistant strain ( p<0.0002) and ceftazidime use and PAGIC caused by a ceftazidime-resistant strain ( p<0.02). The ecological impact of antibiotic use is of great clinical relevance and clinicians should consider antimicrobial resistance in order to limit the development and dissemination of resistant microorganisms. [ABSTRACT FROM AUTHOR]

Rahnella aquatilis was isolated from the blood cultures of two patients who were in different units of the same hospital. Both isolates were susceptible to aminoglycosides, fluoroquinolones, cotrimoxazole, piperacillin, third generation cephalosporins and amoxicillin-clavulanate, but resistant to amoxicillin, ticarcillin, and first generation cephalosporins. The synergistic activity of amoxicillin and clavulanic acid suggested the presence of a Β-lactamase, confirmed by a positive nitrocefin test and by analytical isoelectric focusing. Pulsed-field gel electrophoresis and ribotyping with the pKK3535 probe showed that the isolates shared the same banding pattern. The results of an epidemiological study suggested that an in-house total parenteral nutrition solution might be the source of this unusual gram-negative rod. [ABSTRACT FROM AUTHOR]

The article reports a case study related to Vibrio cholerae O1 bacteremia, with severe sepsis but with no intestinal symptoms, caused by a strain that did not produce the cholera toxin. The case presents important features such as a lack of digestive tract symptoms, a lack of blood dissemination risk factors, the presence of sepsis with fever and skin lesions, and a lack of production of cholera toxin. Vibrio cholerae O1 does not always secrete the cholera toxin. The absence of toxin in this strain was not surprising, since the patient did not present with diarrhea or abdominal symptoms.