23 results on '"Chao, Mei"'
Search Results
2. A new strategy for integrated urban water management in China: Sponge city.
- Author
-
Hao WANG, Chao MEI, JiaHong LIU, and WeiWei SHAO
- Abstract
Urban water-related problems associated with rapid urbanization, including waterlogging, water pollution, the ecological degradation of water, and water shortages, have caused global concerns in recent years. In 2013, in order to mitigate increasingly severe urban water-related problems, China set forth a new strategy for integrated urban water management (IUWM) called the "Sponge City". This is the first holistic IUWM strategy implemented in a developing country that is still undergoing rapid urbanization, and holds promise for application in other developing countries. This paper aims to comprehensively summarize the sponge city. First, this paper reviews prior studies and policies on urban water management in China as important background for the sponge city proposal. Then, the connotations, goals, and features of the sponge city are summarized and discussed. Finally, the challenges, research needs, and development directions pertinent to the sponge city are discussed based on investigations and studies conducted by the authors. The sponge city in China has a short history--given this, there are many issues that should be examined with regard to the stepwise implementation of the Sponge City Programme (SCP). Accordingly, the authors perceive this study as only the beginning of abundant studies on the sponge city. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
3. Inhibitory effect of diosgenin on experimentally induced benign prostatic hyperplasia in rats.
- Author
-
Chen, Jing, Zhang, Huai-fen, Xiong, Chao-mei, and Ruan, Jin-lan
- Abstract
This study investigated the effect of diosgenin, a natural sapogenin possessing various pharmacological activities, on benign prostatic hyperplasia (BPH) in rats and the possible mechanisms. BPH was established in the castrated rats by subcutaneous injection of testosterone propionate. Animals were randomly divided into four groups ( n=10 each): model group (0.5% sodium carboxymethyl cellulose); positive control group (3 mg/kg finasteride); two diosgenin groups (50 and 100 mg/kg). The drugs were intragastricaly given in each group for consecutive 3 weeks. Another 10 rats with no testicles cut off served as negative controls and they were subcutaneously injected with 0.1 mL olive oil per day and then treated with 0.5% sodium carboxymethylcellulose. After 3-week administration, the prostate index and serum PSA level were determined, and histopathological examination was carried out. The levels of MDA, SOD and GPx in prostates were also measured. Additionally, the expression of Bcl-2, Bax and p53 was examined using Western blotting. The results showed that the prostate index and serum PSA level were significantly decreased, and the pathological changes of the prostate gland were greatly improved in diosgenin groups as compared with the model group. Elevated activities of SOD and GPx, and reduced MDA level were also observed in diosgenin-treated rats. In addition, the expression of Bcl-2 in prostates was down-regulated, whereas that of Bax and p53 was up-regulated in diosgenin-treated rats. These results indicated that diosgenin was effective in inhibiting testosterone propionate-induced prostate enlargement and may be a candidate agent for the treatment of BPH. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
4. Incidence of ischemic stroke and systemic embolism in patients with hypertrophic cardiomyopathy, nonvalvular atrial fibrillation, CHADS-VASc score of ≤1 and without anticoagulant therapy.
- Author
-
Yang, Yin-Jian, Yuan, Jin-Qing, Fan, Chao-Mei, Pu, Jie-Lin, Fang, Pi-Hua, Ma, Jian, Guo, Xi-Ying, and Li, Yi-Shi
- Subjects
EMBOLISMS ,TREATMENT of embolisms ,HYPERTROPHIC cardiomyopathy ,ATRIAL fibrillation ,STROKE risk factors ,EMBOLISM risk factors ,ANTICOAGULANTS ,PATIENTS - Abstract
Data on the risk of ischemic stroke and systemic embolism (iSSE) events in patients with nonvalvular atrial fibrillation (NVAF), a CHADS-VASc score of ≤1, hypertrophic cardiomyopathy (HCM), and without anticoagulant therapy are still lacking. The aim of this study was to investigate the incidence of iSSE events in these patients. We consecutively screened medical records of patients with HCM and NVAF referred to Fuwai Hospital between January 1994 and March 2014. The primary end point was iSSE events, defined as a composite of ischemic stroke and systemic embolism. Follow-up was carried out to ascertain end point status. Medical records of 522 patients with NVAF and HCM were screened. A total of 108 patients (20.7 %) with a CHADS-VASc score of ≤1 and without anticoagulant therapy were enrolled and constituted our study population. After a median follow-up of 2.4 years (range 0.6-14.1 years; 376.2 patient-years), ischemic stroke occurred in 2 patients, resulting in death of 1 patient in the first year and paralysis of the other patient in the fourth year. No other iSSE events occurred. The incidence of iSSE was 0.9 % [95 % confidence interval (CI) 0.0-5.0 %] in the first year, and 0.5 % per 100 patient-years (95 % CI 0.1-1.9 %). The risk of iSSE events seems low in patients with NVAF, a CHADS-VASc score of ≤1, HCM, and without anticoagulant therapy. Multicenter studies with sizeable study populations are needed to validate the risk of iSSE events in these patients. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
5. Neuroprotective effect of peptides extracted from walnut ( Juglans Sigilata Dode) proteins on Aβ25-35-induced memory impairment in mice.
- Author
-
Zou, Juan, Cai, Pei-shan, Xiong, Chao-mei, and Ruan, Jin-lan
- Abstract
Alzheimer's disease (AD) is one of the major neurodegenerative disorders of the elderly, which is characterized by the accumulation and deposition of amyloid-beta (Aβ) peptide in human brains. Oxidative streβs and neuroinflammation induced by Aβ in brain are increasingly considered to be responsible for the pathogenesis of AD. The present study aimed to determine the protective effects of walnut peptides against the neurotoxicity induced by Aβ25-35 in vivo. Briefly, the AD model was induced by injecting Aβ25-35 into bilateral hippocampi of mice. The animals were treated with distilled water or walnut peptides (200, 400 and 800 mg/kg, p.o.) for five consecutive weeks. Spatial learning and memory abilities of mice were investigated by Morris water maze test and step-down avoidance test. To further explore the underlying mechanisms of the neuroprotectivity of walnut peptides, the activities of superoxide dismutase (SOD), glutathione (GSH), acetylcholine esterase (AChE), and the content of malondialdehyde (MDA) as well as the level of nitric oxide (NO) in the hippocampus of mice were measured by spectrophotometric method. In addition, the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and IL-6 in the samples were determined using ELISA. The hippocampal expressions of inducible nitric oxide synthase (iNOS) and nuclear factor κB (NF-κB) were evaluated by Western blot analysis. The results showed that walnut peptides supplementation effectively ameliorated the cognitive deficits and memory impairment of mice. Meanwhile, our study also revealed effective restoration of levels of antioxidant enzymes as well as inflammatory mediators with supplementation of walnut peptides (400 or 800 mg/kg). All the above findings suggested that walnut peptides may have a protective effect on AD by reducing inflammatory responses and modulating antioxidant system. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
6. Preparation of magnetic molecularly imprinted polymers for selective isolation and determination of kaempferol and protoapigenone in Macrothelypteris torresiana.
- Author
-
Cai, Pei-shan, Zhao, Yang, Yang, Tong-hua, Chen, Jing, Xiong, Chao-mei, and Ruan, Jin-lan
- Abstract
Novel uniform-sized magnetic molecularly imprinted polymers (MMIPs) were synthesized for selective recognition of active antitumor ingredients of kaempferol (KMF) and protoapigenone (PA) in Macrothelypteris torresiana ( M. torresiana) by surface molecular imprinting technique in this study. Super paramagnetic core-shell nanoparticles (γ-MPS-SiO@FeO) were used as seeds, KMF as template molecule, acrylamide (AM) as functional monomer, and N, N′-methylene bisacrylamide (BisAM) as cross-linker. The prepared MMIPs were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), transmission electron microscopy (TEM) and thermo-gravimetric analysis (TGA), respectively. The recognition capacity of MMIPs was 2.436 times of non-imprinted polymers. The adsorption results based on kinetics and isotherm analysis were in accordance with the pseudo-second-order model ( R=0.9980) and the Langmuir adsorption model ( R=0.9944). The value of E (6.742 kJ/mol) calculated from the Dubinin-Radushkevich isotherm model suggested that the physical adsorption via hydrogen-bonding might be predominant. The Scatchard plot showed a single line ( R=0.9172) and demonstrated the homogeneous recognition sites on MMIPs for KMF. The magnetic solid phase extraction (MSPE) based on MMIPs as sorbent was established for fast and selective enrichment of KMF and its structural analogue PA from the crude extract of M. torresiana and then KMF and PA were detected by HPLC-UV. The established method showed good performance and satisfactory results for real sample analysis. It also showed the feasibility of MMIPs for selective recognition of active structural analogues from complex herbal extracts. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
7. Simultaneous Quantification of Secoisolariciresinol Diglucoside and Cyanogenic Glycosides in Flaxseed Products by Various Processing Methods.
- Author
-
Wang, Ya-Li, Liu, Zhi-Jun, Liu, Qiang, Ma, Jian-Nan, Hattori, Masao, and Ma, Chao-Mei
- Abstract
Two cyanogenic glycosides (linustatin and neolinustatin) and sucrose were isolated from defatted flaxseed, and their structures were determined by NMR spectral analysis and comparison with existing data. Using secoisolariciresinol diglucoside (SDG) and the cyanogenic glycosides as standards, we developed a method to quantify the three compounds simultaneously by ultrahigh-performance liquid chromatography (UHPLC)-MS in selected reaction monitoring (SRM) mode. Using this tool, flaxseed powder-processed by various methods-was analyzed with regard to the contents of the beneficial compound, SDG, and the toxic compounds, cyanogenic glycosides. The levels of SDG in samples treated with alkaline solution are much higher than in samples without treatment, and in general, the levels of linustatin and neolinustatin in flaxseed powder decreased progressively as heating time is prolonged after treatment with alkaline solutions. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
8. Fatty Acid Oxidation Disorders in a Chinese Population in Taiwan.
- Author
-
Chien, Yin-Hsiu, Lee, Ni-Chung, Chao, Mei-Chyn, Chen, Li-Chu, Chen, Li-Hsin, Chien, Chun-Ching, Ho, Hui-Chen, Suen, Jeng-Hung, and Hwu, Wuh-Liang
- Published
- 2013
- Full Text
- View/download PDF
9. Mutation Profile of the MUT Gene in Chinese Methylmalonic Aciduria Patients.
- Author
-
Liu, Mei-Ying, Liu, Tze-Tze, Yang, Yang-Ling, Chang, Ying-Chen, Fan, Ya-Ling, Lee, Shu-Fen, Teng, Yu-Ting, Chiang, Szu-Hui, Niu, Dau-Ming, Lin, Shio-Jean, Chao, Mei-Chun, Lin, Shuan-Pei, Han, Lian-Shu, Qi, Yu, and Hsiao, Kwang-Jen
- Published
- 2012
- Full Text
- View/download PDF
10. Boosting the Performance of Multimedia Applications Using SIMD Instructions.
- Author
-
Weihua Jiang, Chao Mei, Bo Huang, Jianhui Li, Jiahua Zhu, Binyu Zang, Chuanqi Zhu, and Bodik, Rastislav
- Abstract
Modern processors' multimedia extensions (MME) provide SIMD ISAs to boost the performance of typical operations in multimedia applications. However, automatic vectorization support for them is not very mature. The key difficulty is how to vectorize those SIMD-ISA-supported idioms in source code in an efficient and general way. In this paper, we introduce a powerful and ex-tendable recognition engine to solve this problem, which only needs a small amount of rules to recognize many such idioms and generate efficient SIMD in-structions. We integrated this engine into the classic vectorization framework and obtained very good performance speedup for some real-life applications. [ABSTRACT FROM AUTHOR]
- Published
- 2005
11. Dioscin-induced apoptosis of human LNCaP prostate carcinoma cells through activation of caspase-3 and modulation of Bcl-2 protein family.
- Author
-
Chen, Jing, Li, Hui-min, Zhang, Xue-nong, Xiong, Chao-mei, and Ruan, Jin-lan
- Abstract
Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin (1, 2 and 4 μmol/L) could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
12. Screening, purification, and characterization of an extracellular prolyl oligopeptidase from Coprinopsis clastophylla.
- Author
-
Chen, Jen-Tao, Chao, Mei-Li, Wen, Chiou-Yen, and Chu, Wen-Shen
- Abstract
Culture filtrates of 22 mushrooms were screened for extracellular prolyl oligopeptidase activity. Four strains with relatively high enzyme activity were all from inky cap mushrooms. The production of Coprinopsis clastophylla prolyl oligopeptidase was associated with the growth of the fungus and the enzyme was not released by cell lysis. The enzyme was purified 285-fold to a specific activity of 52.05 U/mg. It was purified to a single band on a native polyacrylamide gel. However, the enzyme separated into three bands on a sodium dodecyl sulfate-polyacrylamide gel with mobility corresponding to molecular weights of approximately 84, 60, and 26 kDa. The results of tandem mass spectrometric analysis revealed that the 60 kDa protein was likely a degradation product of the 84 kDa protein. The isoelectric point of the purified enzyme was 5.2. The purified enzyme had an optimal pH and temperature of 8.0 and 37°C, respectively. Diisopropyl fluorophosphate (DFP), p-chloromercuribenzoaic acid (PCMB), Hg, and Cu strongly inhibited C. clastophylla prolyl oligopeptidase. This enzyme is a serine peptidase and one or more cysteine residues of the enzyme are close to the active site. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
13. Polymorphisms of transforming growth factor-β signaling pathway and Kawasaki disease in the Taiwanese population.
- Author
-
Kuo, Ho-Chang, Onouchi, Yoshihiro, Hsu, Yu-Wen, Chen, Wei-Chiao, Huang, Jin-Ding, Huang, Ying-Hsien, Yang, Ya-Ling, Chao, Mei-Chyn, Yu, Hong-Ren, Juan, Yung-Shun, Kuo, Chiu-Ming, Yang, Kuender D, Huang, Jung-San, and Chang, Wei-Chiao
- Subjects
SINGLE nucleotide polymorphisms ,TRANSFORMING growth factors ,CELLULAR signal transduction ,MUCOCUTANEOUS lymph node syndrome ,TAIWANESE people ,VASCULITIS ,HUMAN genetic variation ,DISEASES - Abstract
Kawasaki disease (KD) is a systemic vasculitis associated with cardiovascular symptom. A previous study in the European descent has indicated that genetic variants of the transforming growth factor-β (TGF-β) pathway are involved in the KD susceptibility and clinical status. This study was conducted to investigate if polymorphisms in TGF-β signaling pathway are associated with KD susceptibility, and the coronary artery lesion formation. A total of 950 subjects (381 KD patients and 569 controls) were investigated to identify 12 single-nucleotide polymorphisms in the TGF-β signaling pathway (rs2796817, rs10482751, rs2027567, rs12029576, rs11466480, rs4776338, rs12901071, rs7162912, rs1438386, rs6494633, rs12910698 and rs4776339) by using TaqMan Allelic Discrimination assay. Our results indicated that rs1438386 in the SMAD3 is significantly associated with the susceptibility of KD. Additionally, both haplotypes of TGFβ2 and SMAD3 were also associated with the risk of KD. This study showed that genetic polymorphisms in TGF-β signaling pathway are associated with KD susceptibility, but not coronary artery lesions formation, or intravenous immunoglobulin treatment response in the Taiwanese population. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
14. Lack of Association between ORAI1/CRACM1 Gene Polymorphisms and Kawasaki Disease in the Taiwanese Children.
- Author
-
Kuo, Ho-Chang, Lin, Ying-Jui, Juo, Suh-Hang, Hsu, Yu-Wen, Chen, Wei-Chiao, Yang, Kuender, Liang, Chi-Di, Yang, Shengyu, Chao, Mei-Chyn, Yu, Hong-Ren, Wang, Shouyan, Lin, Li-Yan, and Chang, Wei-Chiao
- Subjects
GENETIC polymorphisms ,MUCOCUTANEOUS lymph node syndrome ,TAIWANESE people ,VASCULITIS ,CORONARY arteries ,IMMUNOGLOBULINS ,DISEASES - Abstract
Objective: Kawasaki disease (KD) is characterized by systemic vasculitis of an unknown cause. A previous study has indicated that a polymorphism of the inositol 1,4,5-trisphosphate 3-kinase C ( ITPKC) gene is involved in the susceptibility to KD. ORAI (also known as CRACM1) is one of the components of store-operated calcium channels involved in regulating immune and inflammatory reactions. This study was conducted to investigate if polymorphisms in ORAI1/CRACM1, a gene downstream from ITPKC, are associated with KD susceptibility and clinical outcomes. Materials and Methods: A total of 1,056 subjects (341 KD patients and 715 controls) were investigated to identify five tagging single nucleotide polymorphisms (tSNPs) in ORAI1/CRACM1 (rs12313273, rs6486795, rs7135617, rs12320939, and rs712853) by using the TaqMan Allelic Discrimination assay. Results: No significant associations between genotype and allele frequency of the five ORAI1/CRACM1 tSNPs were observed in the KD patients and controls. In KD patients, no significant associations between ORAI1/CRACM1 polymorphisms and coronary artery lesion (CAL) formation or intravenous immunoglobulin (IVIG) treatment response were observed. The results from haplotype analysis were insignificant. Conclusions: This study showed for the first time that ORAI1/CRACM1 polymorphisms are not associated with KD susceptibility, CAL formation, or IVIG treatment response in the Taiwanese population. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
15. Metabolism and pharmacokinetics in rats of ganoderiol F, a highly cytotoxic and antitumor triterpene from Ganoderma lucidum.
- Author
-
Zhang, Qun, Zuo, Feng, Nakamura, Norio, Ma, Chao-Mei, and Hattori, Masao
- Abstract
The metabolism of ganoderiol F (GF), a cytotoxic and antitumor triterpene from Ganoderma lucidum, by intestinal bacteria and its pharmacokinetics in rats were investigated by using liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). GF was converted to ganodermatriol by anaerobic incubation with bacterial mixtures from rats and humans. This metabolite was detected in rat feces, but not in plasma and urine, after oral administration of GF. The fate of GF after oral (p.o.) and intravenous (i.v.) administration to rats was examined in pharmacokinetics studies. Plasma samples pretreated by solid-phase extraction were quantified by HPLC/MS/MS over a GF concentration range of 1.25–100 ng/ml (S/N = 5). The intra- and interday precision (CV%) was below 8% and accuracy was within the range of 95.9–103.6% for all samples. The range of recovery ratios was 89.2–98.2%. After the administration of GF at 0.5 mg/kg i.v., the plasma concentrations of GF quickly declined and the elimination half-life values ( t
1/2α and t1/2β ) were about 2.4 and 34.8 min. On the other hand, the elimination half-life values ( t1/2α ) after p.o. administration of GF at doses of 20 and 50 mg/kg were 14.4 and 143.3 min for the former, and 18.6 and 114.6 min for the latter. The AUC0– t value was 11.17 (ng/ml) h at a GF dose of 0.5 mg/kg i.v., but 49.4 and 111.6 (ng/ml) h at GF doses of 20 and 50 mg/kg p.o., respectively, indicating that the AUC0– t value is proportional to the administered oral doses. The estimated absolute bioavailability of GF in rats was F = 0.105. [ABSTRACT FROM AUTHOR]- Published
- 2009
- Full Text
- View/download PDF
16. Triterpenes from Cynomorium songaricium—analysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating.
- Author
-
Ma, Chao-Mei, Wei, Ying, Wang, Zhi-Gang, and Hattori, Masao
- Abstract
Inhibitory activity of the three major triterpenes from the stems of Cynomorium songaricum—ursolic acid, acetyl ursolic acid, and malonyl ursolic acid hemiester—and their related compounds were tested for their inhibitory activity on HCV protease; malonyl ursolic acid hemiester was the most potent. A HPLC–PAD (photo diode array detector)–MS method was established to quantify the contents of each triterpene in C. songaricum. Using this method, the effect heating had on the contents was also investigated. It was found that among the three triterpenes, the content of malonyl ursolic acid hemiester decreased most quickly during the heating process. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
17. Synthesis of mangiferin derivatives as protein tyrosine phosphatase 1B inhibitors.
- Author
-
Hong-gang Hu, Ming-juan Wang, Qing-jie Zhao, Hong-li Liao, Ling-zhi Cai, Yan Song, Jun Zhang, Shi-chong Yu, Wan-sheng Chen, Chao-mei Liu, and Qiu-ye Wu
- Subjects
PROTEIN-tyrosine phosphatase ,GLUCOSIDES ,PHOSPHATASES ,TYPE 2 diabetes ,OBESITY - Abstract
Protein tyrosine phosphatase 1B (PTP1B) has received much attention due to its pivotal role in type 2 diabetes and obesity as a negative regulator of the insulin signaling pathway. Mangiferin, a xanthone glucoside, has been reported to possess significant antidiabetic activity. In the present study, a series of mangiferin derivatives was synthesized and evaluated for their PTP1B inhibitory activity. Some of the screened compounds displayed good PTP1B inhibitory activity. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
18. Identification of four novel mutations in the CYP21 gene in congenital adrenal hyperplasia in the Chinese.
- Author
-
Lee, H.-H., Chao, Hsiang-Tai, Lee, Yann-Jinn, Shu, San-Ging, Chao, Mei-Chyn, Kuo, Jing-Mei, and Chung, Bun-chu
- Abstract
Congenital adrenal hyperplasia (CAH) is a common autosomal recessive disorder mainly caused by defects in the steroid 21-hydroxylase (CYP21) gene. We have analyzed CYP21 gene sequences in 65 CAH families in Taiwan. All ten exons of the CYP21 gene were analyzed by differential polymerase chain reaction followed by single-strand conformation polymorphism electrophoresis and the amplification-created restriction site method. About 95% (123 chromosomes) contain mutations due to conversion of DNA sequences into its neighboring homologous pseudogene, CYP21P. Four novel mutations representing 5% of the total chromosomes have also been identified. The mutations were confirmed by sequencing an aberrant DNA fragment. These four mutations included a base change of the splicing donor site at intron 2 from GT to AT, a base substitution of C to T at codon 316, deletion of ten bases (TCCAGCTCCC) at codons 330–333 of exon 8, and duplication of 16 bases (CCTGGATGACACGGTC) at codons 393–397 of exon 9. The loss of the splicing donor site at intron 2 and the premature stop at codon 316 may result in aberrant splicing to reduce enzyme activity and a truncated protein with no enzyme activity, respectively. Likewise, both the duplication and the deletion forms create a frameshift and premature stop during translation. The resulting proteins lack the heme-binding domain and hence are expected to lose enzymatic activity. Since these mutations are not found in the neighboring CYP21P pseudogene, gene conversion should not be the cause of these novel mutations. [ABSTRACT FROM AUTHOR]
- Published
- 1998
- Full Text
- View/download PDF
19. The impact of a systems approach on interactive distance teaching: The development of the ATM Video Network System at National Chung Cheng University.
- Author
-
Chao, Mei-Sheng
- Subjects
DISTANCE education ,ASYNCHRONOUS transfer mode ,LEARNING ,TEACHERS ,CLASSROOMS ,STUDENTS ,TEACHING aids ,SATISFACTION - Abstract
The article offers information on the development an asynchronous transfer model (ATM) video network system at National Chung Cheng University in Taiwan, and discusses its impact on distance teaching. As mentioned, a survey showed that students in the remote classrooms have higher percentage of dissatisfaction in presentation of learning materials, interaction between students and instructor, and learning achievement, as compared to students in broadcast classrooms. It is also mentioned that network technology can create environments that encourage students to function independently and think critically.
- Published
- 1999
- Full Text
- View/download PDF
20. Chromosomal and biochemical screening on mentally retarded school children in Taiwan.
- Author
-
Wuu, Kuang-Dong, Chiu, Pao-Chin, Li, Shuan-Yow, Chen, Jia-Yuh, Chao, Mei-Chyn, Ko, Fang-Jong, Wang, Tso-Ren, and Hsiao, Kwang-Jen
- Published
- 1991
- Full Text
- View/download PDF
21. Crucial roles of the pentatricopeptide repeat protein SOAR1 in Arabidopsis response to drought, salt and cold stresses
- Author
-
Shang-Chuan Jiang, Kai Lu, Xiao-Fang Wang, Da-Peng Zhang, Shan Liang, Yu Yongtao, Zhen Wu, and Chao Mei
- Subjects
Drought stress ,Salinity ,Arabidopsis thaliana ,Acclimatization ,Arabidopsis ,Pentatricopeptide repeat (PPR) protein ,Germination ,Plant Science ,Genes, Plant ,Real-Time Polymerase Chain Reaction ,Article ,chemistry.chemical_compound ,Salinity stress ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Osmotic Pressure ,Stress, Physiological ,Botany ,Genetics ,Abscisic acid ,Abiotic component ,Regulation of gene expression ,biology ,Arabidopsis Proteins ,fungi ,food and beverages ,General Medicine ,biology.organism_classification ,Plants, Genetically Modified ,ABI1 ,Abscisic acid signaling ,Cell biology ,Droughts ,Cold Temperature ,chemistry ,Pentatricopeptide repeat ,Signal transduction ,Cold stress ,Agronomy and Crop Science ,SOAR1 ,Abscisic Acid ,Signal Transduction - Abstract
Whereas several mitochondrial/chloroplast pentatricopeptide repeat (PPR) proteins have been reported to regulate plant responses to abiotic stresses, no nucleus-localized PPR protein has been found to play role in these processes. In the present experiment, we provide evidence that a cytosol-nucleus dual-localized PPR protein SOAR1, functioning to negatively regulate abscisic acid (ABA) signaling in seed germination and postgermination growth, is a crucial, positive regulator of plant response to abiotic stresses. Downregulation of SOAR1 expression reduces, but upregulation of SOAR1 expression enhances, ABA sensitivity in ABA-induced promotion of stomatal closure and inhibition of stomatal opening, and plant tolerance to multiple, major abiotic stresses including drought, high salinity and low temperature. Interestingly and importantly, the SOAR1-overexpression lines display strong abilities to tolerate drought, salt and cold stresses, with surprisingly high resistance to salt stress in germination and postgermination growth of seeds that are able to potentially germinate in seawater, while no negative effect on plant growth and development was observed. So, the SOAR1 gene is likely useful for improvement of crops by transgenic manipulation to enhance crop productivity in stressful conditions. Further experimental data suggest that SOAR1 likely regulates plant stress responses at least partly by integrating ABA-dependent and independent signaling pathways, which is different from the ABI2/ABI1 type 2C protein phosphatase-mediated ABA signaling. These findings help to understand highly complicated stress and ABA signalling network. Electronic supplementary material The online version of this article (doi:10.1007/s11103-015-0327-9) contains supplementary material, which is available to authorized users.
- Full Text
- View/download PDF
22. Case of chronic lymphocytic leukemia with unusual chromosome aberrations.
- Author
-
Hsiao, Hui-Hua, Hung, Yi-Hui, Hsiao, Hui-Pin, Tseng, Shih-Bin, Tsai, Hui-Jen, Liu, Yi-Chang, Liu, Ta-Chih, Chao, Mei-Chyn, Chang, Yuli, and Lin, Sheng-Fung
- Abstract
Chronic lymphocytic leukemia is one of the most common leukemias in the western world and consists of many chromosome aberrations. We report the case of a 74-year-old male patient with chronic lymphocytic leukemia with complex variant translocations t(8;22)(q24;q11) and der(8)t(6;8)(p21;p21) identified by chromosome banding analysis and confirmed by fluorescence in situ hybridization analysis of interphase cells. Because of the rarity of these changes, possible molecular mechanisms associated with this karyotype are discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
23. Hepato-protective effects and chemical constituents of a bioactive fraction of the traditional compound medicine-Gurigumu-7
- Author
-
Hai-Yan Xu, Qiong Ma, Wang Yali, Chao-Mei Ma, Zhigang Wu, and Jian-Nan Ma
- Subjects
0301 basic medicine ,Male ,Fraction (chemistry) ,Protective Agents ,01 natural sciences ,Antioxidants ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Ursolic acid ,Malondialdehyde ,Medicine ,Animals ,Carbon Tetrachloride ,Transaminases ,Plants, Medicinal ,biology ,Traditional medicine ,business.industry ,Plant Extracts ,Superoxide Dismutase ,General Medicine ,Mongolia ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,030104 developmental biology ,Gurigumu-7 ,chemistry ,Proanthocyanidin ,Alanine transaminase ,Complementary and alternative medicine ,Carbon tetrachloride ,biology.protein ,Methanol ,Bioactive fraction ,business ,Hepatoprotective effect ,Research Article ,Bioactive constituents - Abstract
Background Gurigumu-7 is an important traditional Mongolian medicine frequently used for liver diseases. However, the pharmacological effects and the bioactive constituents are not well understood. Method This research was to use CCl4-induced liver damage in mice to investigate the hepatoprotective effects of Gurigumu-7 and the methanol eluted fraction from a DIAION column of an extract of Gurigumu-7 (MF). The chemical constituents of MF were analyzed by UPLC-MS. Results Pretreated orally with MF (66, 132 and 264 mg/kg) once a day for 4 days dose-dependently suppressed CCl4-induced mice liver histopathological changes and serum aminotransferase activities (alanine transaminase: 1144.0 ± 787.2 v.s. 2461.8 ± 1072.7 U/L, p 30 mg/g), ursolic acid (14.91 mg/g), bidenoside C (8.75 mg/g), and proanthocyanidins (15.64 mg/g in proanthocyanidin A2 equivalent). Conclusion The methanol eluted fraction (MF) from a DIAION column of an extract of the Mongolian medicine-Gurigumu-7 was found to be more hepatoprotective than Gurigumu-7. The results suggested that MF is a promising bioactive fraction for the development of new hepatoprotective medicine with better formulation and quality control properties.
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.