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1. Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.

Author

Christo, Susan N., Nordon, Robert E., Christo, Farid C., Griesser, Hans J., Diener, Kerrilyn R., Brown, Michael P., Vasilev, Krasimir, and Hayball, John D.

Subjects
INTRACELLULAR calcium, RYANODINE receptors, OSCILLATIONS, T cells
Abstract

The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level. [ABSTRACT FROM AUTHOR]

Published
2015
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