1. Interactions between E2F1 and SirT1 regulate apoptotic response to DNA damage.
- Author
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Chuangui Wang, Lihong Chen, Xinghua Hou, Zhenyu Li, Kabra, Neha, Yihong Ma, Shino Nemoto, Finkel, Toren, Wei Gu, Cress, W. Douglas, and Jiandong Chen
- Subjects
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NAD (Coenzyme) , *DNA damage , *APOPTOSIS , *GENE silencing , *GENETIC regulation , *TRANSCRIPTION factors , *ETOPOSIDE - Abstract
The nicotinamide adenine dinucleotide (NAD)-dependent deacetylase Sir2 (silent information regulator 2) regulates gene silencing in yeast and promotes lifespan extension during caloric restriction. The mammalian homologue of Sir2 (SirT1) regulates p53, NF-κB and Forkhead transcription factors, and is implicated in stress response. This report shows that the cell-cycle and apoptosis regulator E2F1 induces SirT1 expression at the transcriptional level. Furthermore, SirT1 binds to E2F1 and inhibits E2F1 activities, forming a negative feedback loop. Knockdown of SirT1 by small interference RNA (siRNA) increases E2F1 transcriptional and apoptotic functions. DNA damage by etoposide causes E2F1-dependent induction of SirT1 expression and knockdown of SirT1 increases sensitivity to etoposide. These results reveal a mutual regulation between E2F1 and SirT1 that affects cellular sensitivity to DNA damage. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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