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The document discusses the importance of antiviral prophylaxis to prevent herpes simplex virus (HSV) and varicella zoster virus (VZV) reactivation in adult patients with newly diagnosed acute leukemia. It highlights the efficacy of antiviral medications like acyclovir in reducing the incidence of symptomatic HSV reactivation during intensive chemotherapy. The study conducted in Italian centers belonging to SEIFEM group revealed a heterogeneous scenario in antiviral prophylaxis policies, with some centers not adhering to current international guidelines. The authors suggest the need for harmonization in applying antiviral prevention approaches and further prospective studies to optimize the schedule and duration of antiviral prophylaxis in different acute leukemia subgroups. [Extracted from the article]

This paper introduces a novel architecture for optimizing radiation shielding using a genetic algorithm with dynamic penalties and a custom parallel computing architecture. A practical example focuses on minimizing the Total Ionizing Dose for a silicon slab, considering only the layer number and the total thickness (additional constraints, e.g., cost and density, can be easily added). Genetic algorithm coupled with Geant4 simulations in a custom parallel computing architecture demonstrates convergence for the Total Ionizing Dose values. To address genetic algorithm issues (premature convergence, not perfectly fitted search parameters), a Total Ionizing Dose Database Vault object was introduced to enhance search speed (data persistence) and to preserve all solutions' details independently. The Total Ionizing Dose Database Vault analysis highlights boron carbide as the best material for the first layer for neutron shielding and high-Z material (e.g., Tungsten) for the last layers to stop secondary gammas. A validation point between Geant4 and MCNP was conducted for specific simulation conditions. The advantages of the custom parallel computing architecture introduced here, are discussed in terms of resilience, scalability, autonomy, flexibility, and efficiency, with the benefit of saving computational time. The proposed genetic algorithm-based approach optimizes radiation shielding materials and configurations efficiently benefiting space exploration, medical devices, nuclear facilities, radioactive sources, and radiogenic devices. [ABSTRACT FROM AUTHOR]

Background: Gerstmann Sträussler Scheinker (GSS) is an inherited, invariably fatal prion disease. Like other human prion diseases, GSS is caused by missense mutations in the prion protein (PrP) gene (PRNP), and by the formation and overtime accumulation of the misfolded, pathogenic scrapie PrP (PrPSc). The first mutation identified in the PRNP gene, and the one blamed as the main cause of the disease, is c.C305T:p.P102L. Methods and results: The Sanger sequencing method was performed on the PRNP gene for the detection of c.C305T:p.P102L mutations in a cohort of 10 subjects; moreover, a study was carried out, using Next Generation Sequencing (NGS), by sequencing a group of genes related to amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), movement disorders and dementia which show a phenotypic profile similar to that of GSS. The results obtained from the study using NGS indicate the potential role of other genetic variants which could contribute to the various GSS phenotypes. Conclusions: In conclusion, we highlight the large clinical variability in subjects presenting with GSS and p.P102L, as well as the hypothesis that the mutation in PrP codon 102 alone is not sufficient to trigger the cardinal clinical signs of the disease; furthermore, we do not exclude the possibility that further genetic variants play a decisive role in the aspects of the various phenotypes with which GSS manifests itself. [ABSTRACT FROM AUTHOR]

Purpose: Automatic robotic platforms for robot-aided spinal surgery are mostly employed for drilling the pedicle screw path and do not adapt the tool rotational speed depending on the variation of the bone density. This feature is highly desirable in control strategies for robot-aided pedicle tapping, which may result in a poor quality thread if the surgical tool speed is not adequately tuned depending on the bone density to be threaded. Therefore, the objective of this paper is to propose a novel semi-autonomous control for robot-aided pedicle tapping that is able to (i) identify the bone layer transition, (ii) adapt the tool velocity depending on the detected bone layer density and (iii) stop the tool tip before propulsion of the bone boundaries. Methods: The proposed semi-autonomous control for pedicle tapping consists of: (i) a hybrid position/force control loop that allows the surgeon to move the surgical tool along a pre-planned axis and (ii) a velocity control loop that allows him/her to finely tune the tool rotational speed by modulating the tool–bone interaction force along the same axis. The velocity control loop integrates also a bone layer transition detection algorithm that dynamically limits the tool velocity depending on the bone layer density. The approach was tested on the Kuka LWR4+ provided with an actuated surgical tapper which was used to tap a wood specimen simulating the bone layer density characteristics and bovine bones. Results: A normalized maximum time delay in the bone layer transition detection of 0.25 was achieved by the experiments. A success rate of 100 % was achieved for all the tested tool velocities. The proposed control achieved a maximum steady-state error of 0.4 rpm. Conclusion: The study demonstrated high capability of the proposed approach to i) promptly detect transition among the specimen layers and ii) adapt the tool velocities depending on the detected layers. [ABSTRACT FROM AUTHOR]

Affective states are psycho-physiological constructs connecting mental and physiological processes. They can be represented in terms of arousal and valence according to the Russel's model and can be extracted from physiological changes in human body. However, a well-established optimal feature set and a classification method effective in terms of accuracy and estimation time are not present in the literature. This paper aims at defining a reliable and efficient approach for real-time affective state estimation. To obtain this, the optimal physiological feature set and the most effective machine learning algorithm, to cope with binary as well as multi-class classification problems, were identified. ReliefF feature selection algorithm was implemented to define a reduced optimal feature set. Supervised learning algorithms, such as K-Nearest Neighbors (KNN), cubic and gaussian Support Vector Machine, and Linear Discriminant Analysis, were implemented to compare their effectiveness in affective state estimation. The developed approach was tested on physiological signals acquired on 20 healthy volunteers during the administration of images, belonging to the International Affective Picture System, conceived for inducing different affective states. ReliefF algorithm reduced the number of physiological features from 23 to 13. The performances of machine learning algorithms were compared and the experimental results showed that both accuracy and estimation time benefited from the optimal feature set use. Furthermore, the KNN algorithm resulted to be the most suitable for affective state estimation. The results of the assessment of arousal and valence states on 20 participants indicate that KNN classifier, adopted with the 13 identified optimal features, is the most effective approach for real-time affective state estimation. [ABSTRACT FROM AUTHOR]

We retrospectively compared baseline laboratory characteristics and PBSC mobilization parameters from these two latter I FLT3 i -mutated AML patient subgroups. Information on peripheral blood stem cell (PBSC) mobilization, at least to obtain autologous back-up harvest, in I FLT3 i -mutated AML patients receiving midostaurin-incorporating consolidation regimens is so far not available. [Extracted from the article]

Characterizing waste ecotoxicity is laborious because of both the undefined nature of environmental samples and the diversity of contaminants that can be present. With regard to these limitations, traditional approaches do not provide information about the nature of the pollution encountered. To improve such assessments, a fluorescent library of 1870 transcriptomic reporters from Escherichia coli K12 MG1655 was used to report the ecotoxic status of environmental samples. The reliability of the approach was evaluated with 6 metallic pollutants (As, Cu, Cd, Hg, Pb, Zn) used alone and in mixture in pure and complex matrices. A total of 18 synthetic samples were used to characterize the specificity of the resulting metallic contamination fingerprints. Metallic contamination impacted 4.5 to 10.2% of the whole transcriptomic fingerprint of E. coli. The analysis revealed that a subset of 175 transcriptomic reporters is sufficient to characterize metallic contamination, regardless of the nature of the sample. A statistical model distinguished patterns due to metallic contamination and provided information about the level of toxicity with 93 to 98% confidence. The use of the transcriptomic assessment was validated for 17 complex matrices with various toxicities and metal contaminants, such as activated sludge, wastewater effluent, soil, wood and river water. The presence of metals and their associated toxicity, which seems linked to their bioavailabilities, were thereby determined. This method constitutes a possible tool to screen unknown complex samples for their metallic status and identify those for which a deeper characterization must be achieved by the use of traditional biosensors and analytical methods. [ABSTRACT FROM AUTHOR]

We show that the tangential speed v ϕ T (t) of a parabolic semigroup (ϕ t) of holomorphic self-maps in the unit disc is asymptotically bounded from above by (1 / 2) log t , proving a conjecture by Bracci. In order to show the proof, we need a result of "asymptotical monotonicity" of the tangential speed for proper pairs of parabolic semigroups with positive hyperbolic step. [ABSTRACT FROM AUTHOR]

Chromosome 21 trisomy or Down syndrome (DS) is the most common genetic cause of intellectual disability (ID). DS is also associated with hypotonia, muscle weakness, autoimmune diseases, and congenital heart disease. C–C chemokine receptor type 3 (CCR3) plays a role in inflammatory, autoimmune, and neuronal migration mechanisms. The present study aimed to evaluate the expression of the CCR3 gene by NGS and qRT-PCR in patients with DS and normal controls (NC). The CCR3 gene was over-expressed in DS patients compared to NC. These data suggest that an over-expression of the CCR3 gene is associated with the phenotype of patients with DS. [ABSTRACT FROM AUTHOR]

Purpose: Early de-tethering procedures are performed on spinal dysraphisms to prevent neuro-urological deterioration caused by growth. Partial lipoma removal may cause delayed deterioration by re-tethering, while complete removal may increase the risk of postoperative worsening. The present study evaluates the risk of postoperative deterioration and the protective potential of intraoperative neurophysiological monitoring (IOM), with a special reference to the conus lipomas treated with the radical approach. Methods: Forty toddlers (< 24 months) underwent complete perioperative neurological and urological assessment, including urodynamic study (UDS). The dysraphisms were subgrouped according to Pang's classification. IOM was applied in all patients: transcranial motor evoked potentials (tMep) combined with mapping were recorded in all cases while bulbocavernosus reflex (BCR) was evaluable just in 7 cases. Results: At preoperative evaluation, 11 children already had UDS impairment and 2 had motor disturbances before neurosurgery. At 1-month follow-up, preoperative motor disturbances were stable, 7/11 UDS alterations normalized, and the remaining 4 were stable. At 6-month follow-up, all motor deficits and 8/11 preoperative UDS alterations had improved. Unfortunately, 7 children with previously normal UDS experienced a new impairment after surgery: 2/7 normalized while 5/7 did not recover. This postoperative permanent urodynamic impairment occurred in 4 chaotic lipoma (CLchaos) and in one terminal myelocystocele (TMC) that means a surgical deterioration rate of 22% for the high risk cases. Conclusions: This small highly selected series confirms that early de-tethering may stop or revert the spontaneous neuro-urological deterioration: in fact, preoperative UDS impairment was frequent (27.5%) and improved in all the low surgical risk cases (limited dorsal myeloschisis, filar, transitional and dorsal lipomas). On the contrary, in CLchaos and TMC, early de-tethering was unable to revert preoperative UDS impairment, and radical surgery carried a high risk of new neuro-urological deterioration directly caused by the operation. In our experience, IOM had a protective role for motor functions, while it was less effective for the neuro-urological ones, probably due to the anesthesiology regimens applied. In conclusion, among the dysraphisms, CLchoas proved to be the worst enemy that often camouflages at MRI. Affording it without all possible IOM weapons carries a high risk to harm the patient. [ABSTRACT FROM AUTHOR]

The current global interest in circular economy (CE) opens an opportunity to make society's consumption and production patterns more resource efficient and sustainable. However, such growing interest calls for precaution as well, as there is yet no harmonised method to assess whether a specific CE strategy contributes towards sustainable consumption and production. Life cycle assessment (LCA) is very well suited to assess the sustainability impacts of CE strategies. This position paper of the Life Cycle Initiative (hosted by UNEP) provides an LCA perspective on the development, adoption, and implementation of CE, while pointing out strengths and challenges in LCA as an assessment methodology for CE strategies. [ABSTRACT FROM AUTHOR]

The potential of front-face fluorescence spectroscopy coupled with chemometric techniques, namely multiple linear regression (MLR) applied on parallel factor (PARAFAC) scores and partial least squares (PLS), was tested on Lebanese olive oil samples possessing natural variability within their chemical parameters. Ninety-six olive oil samples have been harvested at different dates and from two seasons, processed using different extraction methods, collected from different altitudes and other factors that can increase the variability of the samples' chemical composition. Fluorescence excitation-emission matrices (EEM) of the collected samples were measured, and the relationship between them and the chemical parameters was examined. Twenty-two MLR regression models based on PARAFAC scores were generated, the majority of which showed a good correlation coefficient (R > 0.7 for ten predicted variables). A second model using PLS on the unfolded EEM was also conducted to improve the regression and to assess if it can handle the variability in hand. However, similar results, with a slight improvement over the MLR model, were obtained. In a non-experimental design, such variability may hinder the potentials of front-face fluorescence; however average to good MLR and PLS models were obtained, predicting the Lebanese olive oil deterioration quality parameters and fatty acid content. [ABSTRACT FROM AUTHOR]

A patient's conscious decision to voluntarily undergo a therapeutic treatment is called informed consent (IC). The aim of IC is to provide information about health and treatment options and increase patient's participation in healthcare decision-making. IC therefore honors the ethical principles of human autonomy and self-determination. In the field of assisted reproductive technology (ART), the process of communication and mutual understanding between patient and doctor (the IC process) is a current and relevant issue due to its medical, ethical and psychological implications. This exploratory research aims to understand how couples with infertility problems relate to the ART IC, and it proposes the innovative experience of IC conversations with a psychologist. It also provides practical advice on a better use of IC process in ART centers. [ABSTRACT FROM AUTHOR]

The detection of Salmonella spp. in food samples is regulated by the ISO 6579:2002 standard, which requires that precise procedures are followed to ensure the reliability of the detection process. This standard requires buffered peptone water as a rich medium for the enrichment of bacteria. However, the effects of different brands of buffered peptone water on the identification of microorganisms by Raman spectroscopy are unknown. In this regard, our study evaluated the discrimination between two bacterial species, Salmonella enterica and Escherichia coli, inoculated and analyzed with six of the most commonly used buffered peptone water brands. The results showed that bacterial cells behaved differently according to the brand used in terms of biomass production and the spectral fingerprint. The identification accuracy of the analyzed strains was between 85% and 100% depending on the given brand. Several batches of two brands were studied to evaluate the classification rates between the analyzed bacterial species. The chemical analysis performed on these brands showed that the nutrient content was slightly different and probably explained the observed effects. On the basis of these results, Raman spectroscopy operators are encouraged to select an adequate culture medium and continue its use throughout the identification process to guarantee optimal recognition of the microorganism of interest. [ABSTRACT FROM AUTHOR]

Purpose: Material efficiency encompasses a range of strategies that support a reduction of material consumption and waste production from a product's life cycle perspective and which can help the transition towards a circular economy. The aim of this paper is to analyse the state of implementation of material efficiency requirements for products as set out in existing EU Ecolabel criteria, consider possible improvements, identify current limitations and describe potential or existing synergies with other EU policies and initiatives. Methods: Key concepts related to material efficiency have been provided and classified into three groups which are, in order of decreasing priority: reduction, reuse, and recycling/recovery. This classification system has then been used for the analysis of existing requirements set out for different EU Ecolabel products. This includes a description of potential environmental benefits, trade-offs, market barriers and risks. Material efficiency concepts have then been cross-checked with other EU policies and initiatives. Results and discussion: Looking at EU Ecolabel criteria for 26 different product groups revealed a broad range of material efficiency aspects, some of which are influenced by the nature of the product group itself. Some material efficiency aspects were broadly integrated into EU Ecolabel criteria through complementary strategies (e.g. design for durability, recyclability, availability of spare parts, reversible disassembly and provision of information). However, ways to implement additional material efficiency requirements (e.g. minimum lifetime of products) should be sought further. A symbiotic relationship can exist between the EU Ecolabel and many policy tools in the sense that regulatory and standardisation frameworks can offer a robust basis for justifying the integration of material efficiency aspects in the EU Ecolabel, while the EU Ecolabel can explore and promote approaches targeted at front runners in material efficiency aspects in a voluntary manner. Conclusions: The experience gained from implementing material efficiency aspects in the EU Ecolabel could serve as a reference for shaping design, communication or policy initiatives aimed at the promotion of a more circular economy. Attempts to quantify the impacts from material efficiency measures should be also integrated systematically in future research, with the support of tools like life cycle assessment. However, additional considerations of political, technical and socio-economic nature must be considered when assessing the relevance, feasibility and ambition level of any material efficiency–related requirements. [ABSTRACT FROM AUTHOR]

Purpose: One possible reason for the poor uptake of the EU Ecolabel for furniture products may be that the criteria are too complex for applicants. Consequently, it was decided to develop a method which quantifies criteria complexity and subsequently, to propose ways for its reduction, which could be considered in future criteria revision. Methods: The requirements behind the voluntary EU Ecolabel criteria for furniture products set out in Commission Decision (EU) 2016/1332 have been scored with a "criteria complexity index" (CCI), based on the answers to a series of six questions that relate to the effort required for proper assessment and verification. The criteria, and associated CCI values, have been grouped on a per material basis, allowing a "material complexity index" (MCI) to be calculated and consequently, a "furniture complexity index" (FCI) has been calculated as a function of the materials in different furniture products. Results and discussion: Overall, it was found that CCI values can differ depending on the actual supply chain scenario, that textiles and leather had much higher MCI values than all other materials and that the FCI was completely dependent on the materials the furniture product was composed of. FCI values were much lower in general for non-upholstered furniture. Conclusions: The FCI scores can be greatly reduced by the following: (i) using fewer materials in the product; (ii) having shorter supply chains; (iii) using uncoated metals, or at least carrying out coating in-house; (iv) using solid wood instead of wood-based panels; and (v) identifying competent and communicative suppliers who are especially well-informed about chemicals used. Since furniture manufacturers only have limited scope to make these changes, it is more effective to change the way the criteria are structured in future revisions in order to make the criteria more fairly balanced and flexible for potential applicants. Key points about any future restructuring would be to make the chemical requirements more SME friendly and to consider moving away from a rigid pass–fail approach to a more flexible scoring approach. [ABSTRACT FROM AUTHOR]

This study investigates the caregiving expectations of a group of nineteen older Spanish speakers living in Australia from the perspective of familism. Speakers participated in audio-recorded interviews about their caregiving expectations late in life and four key themes were identified: (1) tension between value of familism and the self-reliance approach encouraged in Australian society; (2) fear of burdening their "westernized" children, which resulted in shifted care expectations; (3) fear of ending up in an aged care facility; and (4) the importance of religion in coping with uncertainty about future care arrangements. [ABSTRACT FROM AUTHOR]

Leukemic cells originate from the malignant transformation of undifferentiated myeloid/lymphoid hematopoietic progenitors normally residing in bone marrow. As the precise molecular mechanisms underlying this heterogeneous disease are yet to be disclosed, the identification and the validation of novel actors in leukemia is of extreme importance. Here, we show that KCTD15, a member of the emerging class of KCTD ((K)potassium Channel Tetramerization Domain containing) proteins, is strongly upregulated in patients affected by B-cell type acute lymphoblastic leukemia (B-ALL) and in continuous cell lines (RS4;11, REH, TOM-1, SEM) derived from this form of childhood leukemia. Interestingly, KCTD15 downregulation induces apoptosis and cell death suggesting that it has a role in cellular homeostasis and proliferation. In addition, stimulation of normal lymphocytes with the pokeweed mitogen leads to increased KCTD15 levels in a fashion comparable to those observed in proliferating leukemic cells. In this way, the role of KCTD15 is likely not confined to the B-ALL pathological state and extends to activation and proliferation of normal lymphocytes. Collectively, data here presented indicate that KCTD15 is an important and hitherto unidentified player in childhood lymphoid leukemia, and its study could open a new scenario for the identification of altered and still unknown molecular pathways in leukemia. [ABSTRACT FROM AUTHOR]

Anthropomorphic criteria are widely adopted for developing socially interactive robots since they can improve human capability to interpret and predict robot motion, with an impact on robot acceptability and human–robot interaction safety. Learning by demonstration approaches based on dynamic movement primitives are a suitable solution for planning the robot motion in human-like fashion and endow robots with generalization capabilities and robustness against perturbation. Objective of this work is to propose a new formulation of the learning by demonstration approach based on dynamic movement primitives (DMPs), called hybrid joint/Cartesian DMPs, for redundant robots with the twofold purpose of avoiding obstacles on the path and obtaining anthropomorphic motion in the joint as well as the task space. The proposed approach, suitable for assistive purposes, was experimentally validated on the anthropomorphic robot arm Kuka Light Weight Robot 4+. Trajectories recorded by an optoelectronic system (i.e. the BTS Smart D) from human demonstrators performing point-to-point reaching and pouring tasks were adopted to teach the robot how to move. A comparative analysis with current methods of learning by demonstration in the literature, i.e. Cartesian DMP and inverse kinematics, was performed. Performance indicators were introduced to (1) assess the robot accuracy of the motion performing, (2) evaluate the level of anthropomorphism of the computed motion, (3) measure the success rate in obstacle avoidance. Moreover, questionnaires were administered to ten human subjects who observed robot motion. The obtained results demonstrated that the proposed approach guarantees anthropomorphic motion of the robot both in the joint and in the task spaces ensuring obstacle avoidance along the robot kinematic chain. In particular, the anthropomorphic robot, while operating with the proposed method, exhibits behaviours in the joint space that are similar to the one recorded during the demonstration and has a level of anthropomorphism rather higher than the one obtained with Cartesian DMP and inverse kinematics (a configuration of the robot joints within the physiological limits is always ensured and the maximum convex hull created between the human and the robot arm is 0.0085 m 3 ). In presence of obstacles, an acceptable level of the Cartesian accuracy ( P o s i t i o n e r r < 0.005 m and O r i e n t a t i o n e r r < 0.02 rad) is achieved. The obstacles are avoided with a 100% of success. The questionnaire results showed that the users feel more comfortable and less nervous to interact with a robot that moves in human-like manner. [ABSTRACT FROM AUTHOR]

The combined use of 3D-fluorescence spectroscopy and independent component analysis using a differential fingerprinting approach has been applied with success to detect physiological effects of dimethoate in honeybees. Biochemical determinations combined with the identification of fluorescence zones that may correspond to proteins, NADH or neurotransmitters/neurohormones (octopamine, dopamine and serotonin) related to the physiological stress caused by the pesticide enabled phenomenological modeling of the physiological response in the honeybee using a simple and rapid method. The signals associated with the fluorophores involved in the response to stress were extracted from the fluorescence spectra using an unsupervised algorithm such as independent component analysis. The signals of different neurotransmitters were isolated on separated factorial components, thus facilitating their biochemical interpretation. [ABSTRACT FROM AUTHOR]

We investigated the mechanism of impact of poor communication skills and emotional and behavioural problems in children with ASD (22–61 months) on parental psychological distress. Participants were dyads enrolled in two pilot intervention studies; the dataset includes cross-sectional data at baseline (N = 82). We postulated an indirect effect of child expressive and receptive communication on parent psychological distress, through child emotional and behavioural problems. The effect of receptive skills on parent psychological distress was fully mediated by child emotional problems: lower receptive skills were associated with higher levels of emotional symptoms, which in turn predicted higher parent psychological distress. Expressive skills did not show either direct or indirect effects. Findings are discussed in light of children's marked 'receptive disadvantage' communication profile. [ABSTRACT FROM AUTHOR]

Background: Neuroimaging evidences and previous successful case series of cingulotomy for cancer pain have disclosed the key-role of the dorsal anterior cingulate cortex (ACC) in the generation of the empathic and affective dimension of pain. The aim of this study is to assess the effectiveness and safety of ACC neuromodulation for the treatment of the thalamic pain syndrome (TPS), a chronic neuropathic disease often complicated by severe affective and emotional distress in the long term. Method: From January 2015 to April 2017, 5 patients with pure drug-refractory TPS underwent ACC deep brain stimulation (DBS) at our institution. Quantitative assessment of pain and health-related quality of life were performed 1 day before surgery and postoperatively at 6 and 18 months by using the numeric rating scale (NRS), the 36-item short-form health survey (SF-36), and the McGill pain and the EuroQol5-domain questionnaires. Results: Mean age at surgery was 56.2 years (range, 47–66). NRS score improved by 37.9% at 6 months (range, − 22.2 to − 80%) and by 35% at 18 months (range, − 11.1 to − 80%). At the last follow-up, one patient reported a relevant pain reduction (NRS 2), only complaining of mild pain poorly interfering with activities of daily living. Concomitant improvements in the McGill and EuroQol5-domain pain questionnaires, SF-36 total and sub-item scores were also noticed at each follow-up. No surgical or stimulation-related complications occurred during the study period. Conclusions: ACC DBS may be a safe and promising surgical option to alleviate discomfort and improve the overall quality of life in a patient affected by drug-resistant TPS. Further prospective, larger, and randomized studies are needed to validate these findings. [ABSTRACT FROM AUTHOR]

It has been suggested that the Theory of Mind (ToM) may rely on more basic processes of social cognition, such as action control (e.g., joint action), even if little is known about this relationship. The relationship between ToM and joint action can be studied in patients with Parkinson’s disease (PD), because they are characterized not only by a deficit in ToM (and in its cognitive and affective subcomponents) but also by a deficit in the inhibition of competing responses. Sixty PD patients and 60 matched healthy controls (HCs) performed a go/no-go Flanker task in both joint and individual conditions. Cognitive (Advanced Test or AT) and affective (Emotion Attribution Task or EAT) ToM also were measured. Thirty-five PD patients and matched HCs also performed the standard Flanker task, as a control measure. In patients, only individuals with high AT scores exhibited a joint Flanker effect, whereas in HCs the joint effect was found irrespectively of AT score. Patients with low EAT scores showed a greater interference effect than patients with high scores, whereas the opposite pattern was found for HCs. In regression analysis AT and EAT scores predicted the Flanker effect in the joint condition only. In the standard task, both groups showed a Flanker effect. The role of different fronto-striatal circuits, especially in PD patients, could explain the different involvement of cognitive and affective ToM in joint tasks. The Flanker effect is discussed considering the referential coding account and the attention-focus account as possible candidates to explain joint action effects. [ABSTRACT FROM AUTHOR]

Imbalances between excitatory and inhibitory synaptic transmission cause brain network dysfunction and are central to the pathogenesis of neurodevelopmental disorders. Parvalbumin interneurons are highly implicated in this imbalance. Here, we probed the social behavior and hippocampal function of mice carrying a haploinsufficiency for Ambra1, a pro-autophagic gene crucial for brain development. We show that heterozygous Ambra1 mice (Ambra+/−) are characterized by loss of hippocampal parvalbumin interneurons, decreases in the inhibition/excitation ratio, and altered social behaviors that are solely restricted to the female gender. Loss of parvalbumin interneurons in Ambra1+/− females is further linked to reductions of the inhibitory drive onto principal neurons and alterations in network oscillatory activity, CA1 synaptic plasticity, and pyramidal neuron spine density. Parvalbumin interneuron loss is underlined by increased apoptosis during the embryonic development of progenitor neurons in the medial ganglionic eminence. Together, these findings identify an Ambra1-dependent mechanism that drives inhibition/excitation imbalance in the hippocampus, contributing to abnormal brain activity reminiscent of neurodevelopmental disorders. [ABSTRACT FROM AUTHOR]

Actual indications for surgery in tuberculosis are limited to obtaining a diagnosis, acquiring tissue for culture studies, treating hydrocephalus, aspiring a brain abscess, and reducing intracranial pressure in patients with multiple tuberculomas. Tuberculosis-related movement disorders are usually treated pharmacologically. We report on a child affected by post-tubercular generalized dystonia, who progressed to status dystonicus (SD) and underwent stereotactic bilateral pallidotomy. After surgery, SD resolved, and drugs were rapidly tapered. The successful reversal of SD and the motor improvement observed in our patient demonstrate the safety, feasibility, and clinical efficacy of pallidotomy in post-tuberculous-meningoencephalitis dystonia and SD. [ABSTRACT FROM AUTHOR]

We present a model of bank risk taking and government guarantees. Levered banks take excessive risk as their actions are not fully priced at the margin by debt holders. The impact of government guarantees on bank risk taking depends critically on the portion of bank investors that can observe bank behavior and hence price debt at the margin. Greater guarantees increase risk taking (moral hazard) when informed investors hold a sufficiently large fraction of liabilities. But, otherwise, they reduce risk taking by increasing the profits of the bank (franchise value effect). The results extend to the case in which information disclosure and, thus, the portion of informed investors is endogenous but costly. The model also shows that, when bank capital is endogenous, public guarantees lead unequivocally to an increase in bank leverage and an associated increase in risk taking. The analysis points to a complex relationship between prudential policy and the institutional framework governing bank resolution and bailouts. [ABSTRACT FROM AUTHOR]

In this paper, a novel, robust, and simple method for automatically estimating the hand pose is proposed and validated. The method uses a multi-camera optoelectronic system and a model-based stochastic algorithm. The approach is marker-based and relies on an Unscented Kalman Filter. A hand kinematic model is introduced for constraining relative marker's positions and improving the algorithm robustness with respect to outliers and possible occlusions. The algorithm outputs are 3D coordinate measures of markers and hand joint angle values. To validate the proposed algorithm, a comparison with ground truths for angular and 3D coordinate measures is carried out. The comparative analysis shows the advantages of using the model-based stochastic algorithm with respect to standard processing software of optoelectronic cameras in terms of implementation simplicity, time consumption, and user effort. The accuracy is remarkable, with a difference of maximum 0.035r a d and 4m m with respect to angular and 3D Cartesian coordinates ground truths, respectively. [ABSTRACT FROM AUTHOR]

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Migration is a key cellular function with important implications in cell physiology. Impairment of such function is observed in angiogenesis, cancer, central nervous system development, and many other physiological and pathological events. Serum is considered among the most potent physiological chemotactic stimuli. Transglutaminase 2 (TG2) is involved in most of the mentioned processes, suggesting the hypothesis that TG2 may modulate cell movement and chemotaxis by acting on serum factors. Cell biology and biochemistry studies confirmed this hypothesis, showing that human serum contains potent chemotactic signals significantly impaired by activated TG2. Bioinformatics studies indicated that one potent serum factor potential substrate of TG2-dependent transamidation is platelet-derived growth factor-BB (PDGF-BB). Cell biology and immunometric experiments carried out with U87MG human glioma cell line showed that human recombinant PDGF-BB pre-incubated with calcium-activated TG2 lost about 70 % of its chemotactic activity and antigenicity. These data indicate that PDGF-BB is a substrate of TG2-transamidating activity, and such modification may play a key role in the modulation of PDGF's chemotactic features. Further, these findings suggest a novel point of view to study the extracellular functions of TG2 and to understand how protein signals, such as growth factors and cytokines, act in the extracellular space to reach their specific targets. [ABSTRACT FROM AUTHOR]

It is well known in the field that culture and context play an important role in child rearing and parenting practices. However, many studies do not address the cultural dimension of attachment, although evidence indicates that aspects of early attachment differ across cultures. With the goal of comparing the quality of mother-baby interactions, this study analyzed attachment behaviors and maternal sensitivity in two settings: urban/ non-Mapuche and rural/ Mapuche in Chile. The sample was composed of 34 mother-baby dyads (17 urban/ non-Mapuche and 17 rural/ Mapuche) with 12-month-old children. During a feeding episode, the attachment style of the interaction was assessed with the Attachment during Stress scale, and maternal sensitivity was evaluated with the Child-Adult Relationship Experimental Index-Toddlers (CARE-Index). The groups differed in their interaction attachment style and in affective and proximity modalities: Urban/ non-Mapuche mothers tended to have secure and ambivalent attachment styles, and rural/ Mapuche mothers tended to have avoidant styles. When educational level is controlled, ethnicity group can explain the differences in mothers' attachment styles. The findings of the study and their implications are discussed. [ABSTRACT FROM AUTHOR]

Background: Hardware-related complications frequently occur in deep brain stimulation. Microscopy and spectroscopy techniques are effective methods for characterizing the morphological and chemical basis of malfunctioning DBS electrodes. A previous report by our team revealed the morphological and chemical alterations on a malfunctioning explanted electrode when it was compared to a new device. The aim of this preliminary study was to verify whether these morphological and chemical alterations in the materials were a direct result of the hardware malfunctioning or if the failure was correlated to a degradation process over time. Methods: Two DBS electrodes were removed from two patients for reasons other than DBS system impairment and were analyzed by a scanning electron microscope and by an energy-dispersive X-ray spectroscopy. The results were compared to a malfunctioning device and to a new device, previously analyzed by our group. Results: The analysis revealed that the wear of the polyurethane external part of all the electrodes was directly correlated with the duration of implantation period. Moreover, these alterations were independent from the electrodes functioning and from parameters used during therapy. Conclusions: This is the first study done that demonstrates a time-related degradation in the external layer of DBS electrodes. The analyses of morphological and chemical properties of the implanted devices are relevant for predicting the possibility of hardware's impairment as well as to improve the bio-stability of DBS systems. [ABSTRACT FROM AUTHOR]

The ability of the liver to regenerate and adjust its size after two/third partial hepatectomy (PH) is impaired in old rodents and humans. Here, we investigated by microarray analysis the expression pattern of hepatic genes in young and old untreated mice and the differences in gene expression profile following PH. Of the 10,237 messenger RNAs that had detectable expression, only 108 displayed a greater than 2-fold modification in gene expression levels between the two groups. These genes were involved in inflammatory and immune response, xenobiotics, and lipid and glucose metabolism. To identify the genes responsible for the different regenerative response, 10-week and 18-month-old mice subjected to PH were sacrificed at different time intervals after surgery. The results showed that 2463 transcripts had significantly different expression post PH between the two groups. However, in spite of impaired liver regeneration in old mice, cell cycle genes were similarly modified in both groups, the only exception being cyclin D1 gene which was up-regulated soon after PH in young mice, but mostly down-regulated in aged animals. Surprisingly, while in young hepatectomized mice, Yap messenger RNA (mRNA) expression was not significantly enhanced and protein expression essentially reflected the progression into cell cycle, its mRNA and protein levels were robustly increased in the liver of aged animals. Furthermore, a significant change of the age-related expression of the size regulator Yes-associated protein (YAP) was observed. Unexpectedly, while in young hepatectomized mice, Yap mRNA expression was not significantly enhanced and protein expression essentially reflected the progression into cell cycle, its mRNA and protein levels were robustly increased in the liver of aged animals. Moreover, when PH was performed on mitogen-induced enlarged livers, the earlier restoration of the original liver mass compared to animals subjected to PH only led to YAP down-regulation concomitantly with cyclin D1 up-regulation. Our data suggest that YAP activation is a size-dependent homeostatic mechanism that does not necessarily reflect cell cycle progression. [ABSTRACT FROM AUTHOR]

Lipid oxidation leads to the formation of volatile compounds involved in the aroma of edible oils. Specifically, the use of rapeseed oil for frying causes an emission of off-flavors, characterized by a fishy odor. The aim of the present study was to identify the compounds present in the headspace of degraded rapeseed oil, and in particular, to determine the presence of six compounds commonly considered as responsible for the fishy odor: 1-penten-3-one, 1-octen-3-one, (Z)-4-heptenal, (E,Z)-2,6-nonadienal, (E,Z)-2,4-heptadienal, and (E,Z,Z)-2,4,7-decatrienal. Because of the complexity of the headspace of degraded oils, comprehensive two-dimensional gas chromatography (GC × GC) coupled to mass spectrometry (MS) was the technique of choice, providing a suitable separation power and sensitivity. The extraction of volatile compounds was performed by headspace solid-phase microextraction (HS-SPME). First, three different column sets were evaluated (apolar-polar, polar-apolar, apolar-medium polar) by means of a mixture of 33 standards representative of those involved in the aroma of edible oils. The apolar-polar column set was considered as providing a satisfactory separation with an excellent occupation of the separation space. The developed method was then applied to a real sample of heated rapeseed oil and 110 compounds were identified, which included the six compounds of interest. The contribution of GC × GC in terms sensitivity was evaluated by means of a comparison with analyses in 1D-GC. Finally, the concentrations of the compounds involved in the fishy odor in heated oil were determined. [ABSTRACT FROM AUTHOR]

Hemifacial spasm (HFS) is generally caused by a neurovascular conflict (NC) at the root exit zone (REZ) of the facial nerve at the brainstem. Although a direct compression to the seventh cranial nerve (CN) by the anterior inferior cerebellar artery (AICA) is generally the most frequent cause, secondary HFS may be related to other pathological conditions. HFS due to an intracranial mass lesion is exceptionally rare and it has been reported in very few cases. The online database was searched for English-language articles reporting cases of HFS due to brainstem mass lesions and the possible pathophysiological mechanisms involved in its genesis. A 47-year-old man affected by an anaplastic astrocytoma of the brainstem at the level of the ponto-medullary junction developed right HFS. He underwent a subtotal surgical removal of the tumor with complete resolution of the HFS. This is the ninth reported case of HFS caused by an intrinsic brainstem tumor. The exceptional rarity of the relationship between intra-axial tumors and peripheral HFS was analyzed. [ABSTRACT FROM AUTHOR]

Fluorescein is widely used as a fluorescent tracer for many applications. Its capacity to accumulate in cerebral areas where there has been blood-brain barrier damage makes it particularly suitable as a dye for the intraoperative visualization of malignant gliomas (MGs). In this report, we describe the results of a comprehensive review on the use of fluorescein in the surgical treatment of MGs. A comprehensive literature search and review for English-written articles concerning the use of fluorescein in the resection of MGs has been conducted. The search was executed through a PubMed literature search using the following keywords: malignant gliomas, glioblastomas, high-grade gliomas, YELLOW 560, total removal, dedicated filter, neurosurgery, brain tumors, intracranial tumors, and confocal microscopy. The literature search resulted in the retrieval of 412 evidence-based articles. Of these, 17 were found to be strictly related to the resection of MG with the aid of fluorescein. In addition to these 17, we have included 2 articles derived from a personal database of the corresponding author (FA). The analysis of the articles reviewed revealed three major applications of fluorescein during surgery for MGs that was documented: Fluorescein-guided resection of MGs with white-light illumination, fluorescein-guided resection of MGs with a surgical microscope equipped with a dedicated filter for fluorescein, and confocal microscopy for intraoperative histopathological analysis on MGs. The systemic review conducted on the use of fluorescein in MGs explored the applications and the different modalities in which fluorescein has been used. The data we have gathered indicates that fluorescein-guided surgery is a safe, effective, and convenient technique to achieve a high rate of total removal in MGs. Further prospective comparative trials, however, are still necessary to prove the impact of fluorescein-guided surgery on both progression-free survival and overall survival. [ABSTRACT FROM AUTHOR]