1. The age and genomic integrity of neurons after cortical stroke in humans.
- Author
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Huttner, Hagen B, Bergmann, Olaf, Salehpour, Mehran, Rácz, Attila, Tatarishvili, Jemal, Lindgren, Emma, Csonka, Tamás, Csiba, László, Hortobágyi, Tibor, Méhes, Gábor, Englund, Elisabet, Solnestam, Beata Werne, Zdunek, Sofia, Scharenberg, Christian, Ström, Lena, Ståhl, Patrik, Sigurgeirsson, Benjamin, Dahl, Andreas, Schwab, Stefan, and Possnert, Göran
- Subjects
NUCLEIC acids ,GENES ,NEURONS ,GENOMES ,CEREBRAL cortex - Abstract
It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived
14 C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival. [ABSTRACT FROM AUTHOR]- Published
- 2014
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