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study on the characterization of Tunisian extra-virgin olive oil varieties produced in their place of origin has been carried out. Due to the influence of the genotype and environmental, agronomic and technological factors on the chemical composition of olive oil and its quality, all the olives studied were collected on the same season, and the oil was obtained under the same processing technique. Several analyses were performed to characterize the different olive oils: free acidity, peroxide value, fatty acid composition, radical scavenging activity, Rancimat assay, pigments content and phenolic compounds by HPLC-MS. In order to evaluate all the results obtained (36 parameters for each variety), different statistical analyses were used to discriminate the extra-virgin olive oil varieties: one-way analysis of variance was performed to check significant differences among cultivars ( p ≤ 0.05); PCA was applied to the data showing that variables such as oleic, linoleic, quinic and vanillic acids, apigenin, luteolin, taxifolin, oleuropein aglycon, pinoresinol acetate, elenolic acid and oxidative stability allowed discriminating among the different varieties of extra-virgin olive oil studied. Besides, LDA model was able to classify the samples depending on their geographical origin in Tunisia (North, Centre and South). [ABSTRACT FROM AUTHOR]