Search

Objective: To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma. Methods: The inhibitory effect of TAAs was demonstrated in H-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis. Results: The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC of TAAs on Bel-7402 was 20.06 µg/mL (15.13-26.61µg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs. Conclusion: TAAs is effective for hepatocarcinoma, via inhibiting proliferation and inducing apoptosis. [ABSTRACT FROM AUTHOR]

The article presents the findings of an experimental work and research on the effects of L. F04, the active fraction of the herb Lycopus lucidus, on erythrocytes rheological property, conducted by Shi Hong-zhi, Li Yong-zhi et al. Lycopus lucidus has effects in resisting coagulation and thrombosis. Cell rheology detector type LG-B-190 manufactured by Shidi Scientific Instrument Co. is one of the instruments used in the study. After being injected with dextran 500, rats in the model group showed characteristics of blood stasis.