Search

The Apoptosis is an intensely studied mechanism for cancer therapy. Various genes, drugs, and molecules have been combined to potentiate the apoptotic effect against tumor cells. Magnetic nanocomplexes with biopolymers have been proposed as nanocarriers for delivery of active molecules and improve apoptosis induction. To these purpose were formulated magnetic nanoparticles with curcumin coated chitosan (MNPs-CHI-TPP-CUR) and magnetic nanoparticles with pCEM-TRAIL plasmid coated chitosan (MNPs-CHI-TPP-TRAIL) for use alone or in combination to induce the apoptosis of B16F10 tumor cells because curcumin is capable of overexpressing receptors TRAIL required for apoptosis induction. Also, curcumin (CUR) has been used as a photosensitizer in photodynamic therapy (PDT). In this work, it was produced and characterized nanoparticles with curcumin and nanoparticles with TRAIL gene. Both types of nanoparticles were ~ 200 nm size. The results showed that the combination of (MNPs-CHI-TPP-CUR) and (MNPs-CHI-TPP-TRAIL) induced a significant increase in cellular death within 48 h compared with the effect of each nanocomplex individually, demonstrating a synergic effect. This is the first description the synergic interaction between TRAIL gene and curcumin drug to improve the apoptosis in cancer cells. Also, it was determined that cellular death was potentiated when the treated cells were exposed to laser photoactivation with wavelengths of 405 nm and 532 nm for 15 min. [ABSTRACT FROM AUTHOR]

The accumulation of potential toxic elements (PTEs) in soils is usually conditioned by parental material or anthropogenic sources. To achieve correct land management and land degradation neutrality, it is necessary to spatially detect them. However, there are several areas over the world with high concentrations of PTE but without efficient maps and tools to correctly find solutions and apply control measures. The current study attempts to identify the concentrations, sources, and spatial distributions of the main PTEs such as As, Cd, Cr, Cu, Ni, Pb, and Zn in a non-explored area combining fieldwork and geostatistical analysis. In order to accomplish this goal, a total of 90 soil samples were collected in agricultural and natural areas in the Piedemonte Llanero, Colombia. The chemical analysis was conducted by acid digestion and determined through ICP-OES. Then, ordinary kriging was applied to spatially analyze the most vulnerable areas. Our results demonstrated the effectiveness of these techniques and it is noted that the agricultural areas presented the highest concentrations and represented the potential source of PTEs. On the other hand, the natural areas presented the following concentrations of PTEs Cr (17.10 mg/kg), As (2.92 mg/kg), Cu (7.57 mg/kg), Ni (8.63 mg/kg), Cd (0.17 mg/kg), Pb (8.80 mg/kg), and Zn (27.57 mg/kg) lower than agricultural soils. This information was a key first step to be presented to the policymakers and stakeholders to organize soil sustainable management plans for the Piedemonte Llanero in Colombia. [ABSTRACT FROM AUTHOR]

The main purpose of the present study, conducted in the Piedemonte Llanero of Colombia, was to identify the role of covariates that influence soil properties under different land uses by analyzing the dynamics of selected soil properties in natural and agricultural areas. Soil samples were collected from 90 different sites throughout the region, 45 from areas with agricultural activities and 45 natural areas or areas with low anthropic intervention. The studied soils were strongly acidic (pH < 5.5), had low organic matter contents (1.73% and 2.34% on average in agricultural soils and natural areas, respectively) and, in general, represented poor conditions for agricultural production. The use of multivariate analysis showed that altitude was an important influence on soil properties. Moreover, it was found that land use influenced soil properties, which may limit productive capacity. The results presented here help to clarify the relationships between soils, climate, and landforms in an equatorial climate. [ABSTRACT FROM AUTHOR]

Melanaphis sacchari Zehntner (Hemiptera: Aphididae) is currently the most destructive pest insect of the sorghum crop (Sorghum bicolor L. Moench) and has caused economic losses worldwide. Strategies using natural products such as plant extracts can offer alternatives for the control of this pest. In this investigation, we evaluated the insecticidal effect of the hexane extract of Ricinus communis L. and Momordica charantia L. seeds on M. sacchari in contact bioassays. The results showed that the extract of R. communis presented a mortality rate of 100% in 48 h at 10,000 ppm, while M. charantia presented 80% in 72 h, at 10,000 ppm. Gas chromatography—mass spectrometry (GC–MS) identified the major compounds as squalene in R. communis (51.57%) and linalool oxide in M. charantia (30.45%). The hexanic extracts of these plant species could be considered an ecologically friendly alternative to control this pest, given the use of synthetic chemical insecticides. [ABSTRACT FROM AUTHOR]

Background: Few publications have assessed long-term results of distal locking of short endomedullary nails for extracapsular hip fracture. Virtually all of them focus on immediate differences. Criteria for the use of static or dynamic locking are unclear in most nailing systems, and use is advised in unstable fracture patterns or with risk of bell-clapper effect, but often influenced by the "orthopaedic school".Materials and Methods: This is a historical cohort study on patients diagnosed and operated in 2014 and followed up until endpoint, considered as consolidation or major complication, plus evaluation of overall long-term survival. They were categorised as static distal locking (ST) or dynamic distal locking (DN). Both are comparable, except for all stable pre-operative classifications, Fracture Mobility Score (FMS) at discharge, and immediate post-operative loading, all of which were in favour of DN.Results: Consolidation took place in > 95% of patients, with a non-statistically significant delay trend in ST. Less than 6% in both ST and DN had major complications, with no differences. Most cases suffered early cut-out. Significant fracture collapse was the most frequent minor complication. There were more statistically significant minor and total complications in ST. Infection, without differences, can precede cut-out. Lateral thigh pain was similar and could be related to back-out. In DN, 21.1% of cases were truly dynamised. We did not find differences in mobility or in long-term survival.Conclusions: Any type of distal locking seems to be safe for consolidation, despite a slightly longer consolidation time in static locking. Early cut-out was the main complication, while others were very infrequent, which is an advantage over helical blade devices. There was a higher rate of minor and overall mechanical complications in ST, but infection and lateral thigh pain were similar. Most non-traumatic mechanical complications occurred around 5-6 weeks. About one in five of the DN truly dynamised, with all cases occurring before 8 weeks. Mobility until endpoint and overall long-term survival were not influenced by the locking mode used.Level Of Evidence: Therapeutic study, level 2b. [ABSTRACT FROM AUTHOR]

The aim of this paper is to examine differences in life expectancy (LE) between self-employed (SE) and paid employee (PE) workers when they become retirement pensioners, looking at levels of pension income using administrative data from Spanish social security records. We draw on the Continuous Sample of Working Lives (CSWL) to quantify changes in total life expectancy at age 65 (LE65) among retired men over the longest possible period covered by this data source: 2005–2018. These changes are broken down by pension regime and initial pension income level for three periods. The literature presents mixed evidence, even for the same country–for Japan and Italy, for example–with some studies pointing to higher life expectancy for SE than for PE retirement pensioners while others argue the opposite. In Spain, LE65 is slightly higher for the SE than for PE workers when retirement pensioners. For 2005–2010, a gap in life expectancy of 0.23 years between SE and PE retirement pensioners is observed. This widens to 0.55 years for 2014–2018. A similar trend can be seen if pension income groups are considered. For 2005–2010, the gap in LE65 between pensioners in the lowest and highest income groups is 1.20 years. This widens over time and reaches 1.51 years for 2014–2018. Although these differences are relatively small, they are statistically significant. According to our research, the implications for policy on social security are evident: differences in life expectancy by socioeconomic status and pension regime should be taken into account for a variety of issues involving social security schemes. These include establishing the age of eligibility for retirement pensions and early access to benefits, computing the annuity factors used to determine initial retirement benefits and valuing the liabilities taken on for retirement pensioners. [ABSTRACT FROM AUTHOR]

There is a significant body of research in "naturalistic designs" of augmented reality (AR), concerning different fields (medicine, education, arts etc). Although naturalistic approaches have the potential to enable new forms of experiencing and experimenting with AR technologies, it remains unclear how they can impact participants' motivation. From within an evaluation of the relevant literature and research results pertaining to naturalistic approaches to AR technology, this study aims to show how naturalistic approaches can be particularly effective in increasing the usefulness of the three widely recognized types of AR (marker-based AR, markerless and location-based AR). This study presents the results of an analysis of a review of articles of the peer-reviewed literature on naturalistic approaches applied to AR, considering the advantages, disadvantages and effectiveness of the combination of naturalistic approaches with AR across various domains. In total 33 studies published in peer-reviewed journals and conferences were analyzed. The implications of this research are that naturalistic approaches applied to AR technology help to foster positive attitudes towards AR, to facilitate collaboration and to enhance the users' social collaboration, personal development and skills in the use of AR software. Among all domains of applications of AR with naturalistic approaches (education, medicine, digital arts, cultural heritage), the domain of human-computer interaction has attracted more attention in experimental researches. Also, motion-sensing input devices are the kind of technology which appears more beneficial for these fields. [ABSTRACT FROM AUTHOR]

The use of bryophytes as an indicator of environmental quality has been addressed on numerous occasions and in different places of the world under a variety of conditions. However, in Latin America their use is still limited. In the study described here, the presence and distribution of the bioaccumulation of lead in bryophytes has been evaluated in both contaminated and uncontaminated sites in Villavicencio (Colombia) and its surroundings. Villavicencio is a medium-sized city that is similar in size to many other cities in Latin America. A total of 52 samples of bryophytes were collected, of which 43 belong to points distributed in urban areas of the city (residential, commercial, highway), and the remaining nine are from uncontaminated areas (reference) taken in the surroundings of the city. The samples were treated with acid (nitric and hydrochloric) and subsequently measured by atomic absorption spectrometry. It was found that Pb concentrations in the commercial sector are between 1 and 6 times higher than in the residential and highway sectors. Spatial distribution maps of lead over the sampled territory were created using Arc-GIS 10.1. It is noteworthy that the values obtained are higher than those found in European cities. [ABSTRACT FROM AUTHOR]

Urbanization-induced land-use changes cause several environmental problems, especially in developing countries due to a lack of sufficient urban planning. This study was performed in a medium-size city of Villavicencio, Colombia. Copper, lead, nickel, zinc, chromium, manganese, and cadmium in road-deposited sediment (RDS) from different land uses were determined. Multiple geo- and statistical approaches of geographic information system mapping, Pearson correlation, Kruskal–Wallis H, hierarchical cluster analysis (HCA), and principal component analyze (PCA) were employed to assess the influence of land use on the metals' spatial distribution. The enrichment of given metals in RDS was evaluated by geo-accumulation (Igeo) and pollution load (PLI) indexes. The exposure human health risk was assessed by hazard index (HI). Results show that the average contents of the given metals decreased in the order of commercial > residential > highway > government institutions and public parks areas. Commercial areas thereafter always have the highest metals enrichment (Igeo) and pollution level (PLI). HI assessment indicates that child has a higher health risk than adult due to the exposure to metals in RDS. HCA analysis reveals that surface roughness had a more direct influence than land-use type on metals' distribution. Kruskal–Wallis H test further suggests land-use type had a significant influence on certain metals' spatial variation. Two potential (group) sources of geochemical and vehicular sources, along with leaded petrol and paintings, were inferred to be the main contributors to metals in RDS by PCA analysis. [ABSTRACT FROM AUTHOR]

Breast cancer patients may express abnormal cellular immune responses affecting their immunological competence. The analysis of immunological parameters may be useful as indicators of T cell function. To determine the expression of lymphocyte activation proteins and cytokines in tumor and non-metastatic axillary lymph nodes, 30 breast cancer patients were monitored. CD3 polypeptides, PTKs (protein tyrosine kinases) and phosphorylated tyrosines were studied by Western Blot and cytokines mRNA expression was determined by RT-PCR (reverse transcription-polymerase chain reaction). This group of patient had shown high immunohistochemistry expression of IL-10 in tumors. Activation proteins were mainly expressed in involved lymph nodes comparing with their expression in tumors. The differences in expression of CD3 polypeptides and p56lck between both locations were significant. There was no statistical association between PTKs and IL-10 in the tumor but more than 50% of cases who express IL-10 lost p56lck, p59fyn. A direct association between IL-10 and CD3-polypeptides was observed, however 52.2% of patients who express IL-10 did not express 41 kDa CD3-ζ form. IL-10 mRNA was detected in more than 50% of tumors contrary to the prevalence of type 1 cytokines in regional nodes (40%). The lack of expression of lymphocyte activations proteins and the high expression of IL-10 suggest a downregulation on T cells function in the tumors. These results are useful in order to understand the local immune response that would be key in the control of the tumor progression. [ABSTRACT FROM AUTHOR]