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Micro devices have been widely used in aerospace engineering for years. Engineers are interested in applications of micro devices such as microjets, micro actuators, and micronozzles. The small size nozzles can be used for attitude adjustment and propulsion of micro-satellites or mini-spacecraft. In this paper, convergent-divergent micronozzles have been investigated at supersonic speed with various total pressures and Reynolds numbers. The throat of the micronozzle is 250 micron wide and the nozzle is designed as de Laval type. For the measurements, the Reynolds number at the throat varies from 1200 to 11000 and total pressure varies from 6 psia to 55 psia. Experimental results are obtained with pressure-sensitive paint for pressure measurement and schlieren imaging for flow visualization. Flow visualization is a challenge for conventional techniques due to the small length scales and small depth of the density gradient. A modified schlieren technique is used to increase the sensitivity by taking the ratio of wind-on and wind-off images. Pressure-sensitive paint is also used to obtain global pressure measurement of the flow field and to compare with the schlieren results. [ABSTRACT FROM AUTHOR]

The fluidic oscillator is a device that generates an oscillating jet when supplied with fluid at pressure. The oscillator has no moving parts — the creation of the unsteady jet is based solely on fluid-dynamic interactions. Fluidic oscillators can operate at frequencies ranging up to 20 kHz, and are useful for flow control applications. The fluidic oscillator evaluated in the current study is comprised of two fluid jets that interact in an internal mixing chamber, producing the oscillating jet at the exit. Both porous pressure-sensitive paint (PSP) and dye-colored water flow are used to visualize the internal and external fluid dynamics of the oscillator. Porous PSP formulations have been shown to have frequency responses on the order of 100 kHz, which is more than adequate for visualizing the fluidic oscillations. In order to provide high-contrast PSP data in these tests, one of the internal jets of the fluidic oscillator is supplied with oxygen, and the other with nitrogen. Results indicate that two counter rotating vortices within the mixing chamber drive the oscillations. It is also shown that the fluidic oscillator possesses excellent mixing characteristics. [ABSTRACT FROM AUTHOR]

Osteopenia is a complicating problem that may occur during and after treatment for childhood malignancy. Clinical studies suggest that chemotherapeutic agents directly affect osteoblasts in vivo. Since combinations of agents are used for treatment, we individually investigated the chemosensitivity of human osteoblast-like cells to 11 of the chemotherapeutic agents used. The relative chemosensitivity of osteoblast-like cells representing different stages of cell differentiation was also examined. Cell numbers were evaluated following culture of an established human osteoblast-like cell line (MG63) for 3 days with clinically relevant concentrations of the chemotherapeutic agents. The chemosensitivity of MG63 cells was compared to that of a human osteoprogenitor cell line (HCC1) and primary osteoblast-like (HOB) cells derived from pediatric bone. Cell numbers were reduced by all agents in all cell types, although there was a varied response between agents at equimolar concentrations. In MG63 cells the lowest concentration of agent significantly reducing cell numbers varied between agents, for example, methotrexate (10?7 M), vincristine (10?9 M), and etoposide (10?7 M) (all P <0.01). The less differentiated osteoblast phenotypes were significantly more chemosensitive at equimolar concentrations of methotrexate, vincristine, asparaginase, and dexamethasone than more differentiated phenotypes (all P <0.01). Furthermore, four agents significantly increased alkaline phosphatase (AP) activity in HOB cells. We conclude that individual chemotherapeutic agents added to osteoblast cell cultures reduce cell numbers, with osteoblast precursor cells being preferentially depleted. These results suggest that most of the agents may contribute to osteopenia in childhood malignancy by direct effects on cell numbers. [ABSTRACT FROM AUTHOR]