1. New ambuic acid derivatives from the solid culture of Pestalotiopsis neglecta and their nitric oxide inhibitory activity.
- Author
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Qi, Qiu-Yue, Li, Er-Wei, Han, Jun-Jie, Pei, Yun-Fei, Ma, Ke, Bao, Li, Huang, Ying, Zhao, Feng, and Liu, Hong-Wei
- Subjects
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ASCOMYCETES , *CHEMICAL derivatives , *NITRIC oxide , *NUCLEAR magnetic resonance , *CIRCULAR dichroism - Abstract
Four new ambuic acid derivatives (1-4), and four known derivatives (5-8), were isolated from the solid culture of a plant pathogenic fungus Pestalotiopsis neglecta. Their structures were elucidated by extensive NMR experiments. The absolute configuration of the C-16 secondary alcohol in 1 was deduced via the CD data of the in situ formed [Rh2(OCOCF3)4] complex with the acetonide derivative of 1. The absolute configuration in 3 was assigned by comparison of the experimental and simulated electronic circular dichroism (ECD) spectrum. The NMR data of compound 5 was reported for the first time. In the nitric oxide (NO) inhibition assay, compounds 4, 6 and 7 showed inhibitory activity against the NO production in the lipopolysaccharide (LPS)-induced macrophage with IC50 values of 88.66, 11.20, and 20.80 µM, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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