272 results on '"Ishikawa T"'
Search Results
2. Enhancing rare variant interpretation in inherited arrhythmias through quantitative analysis of consortium disease cohorts and population controls
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Walsh R, Lahrouchi N, Tadros R, Kyndt F, Glinge C, Postema PG, Amin AS, Nannenberg EA, Ware JS, Whiffin N, Mazzarotto F, Škoric-Milosavljevic D, Krijger C, Arbelo E, Babuty D, Barajas-Martinez H, Beckmann BM, Bézieau S, Bos JM, Breckpot J, Campuzano O, Castelletti S, Celen C, Clauss S, Corveleyn A, Crotti L, Dagradi F, de Asmundis C, Denjoy I, Dittmann S, Ellinor PT, Ortuño CG, Giustetto C, Gourraud JB, Hazeki D, Horie M, Ishikawa T, Itoh H, Kaneko Y, Kanters JK, Kimoto H, Kotta MC, Krapels IPC, Kurabayashi M, Lazarte J, Leenhardt A, Loeys BL, Lundin C, Makiyama T, Mansourati J, Martins RP, Mazzanti A, Mörner S, Napolitano C, Ohkubo K, Papadakis M, Rudic B, Molina MS, Sacher F, Sahin H, Sarquella-Brugada G, Sebastiano R, Sharma S, Sheppard MN, Shimamoto K, Shoemaker MB, Stallmeyer B, Steinfurt J, Tanaka Y, Tester DJ, Usuda K, van der Zwaag PA, Van Dooren S, Van Laer L, Winbo A, Winkel BG, Yamagata K, Zumhagen S, Volders PGA, Lubitz SA, Antzelevitch C, Platonov PG, Odening KE, Roden DM, Roberts JD, Skinner JR, Tfelt-Hansen J, van den Berg MP, Olesen MS, Lambiase PD, Borggrefe M, Hayashi K, Rydberg A, Nakajima T, Yoshinaga M, Saenen JB, Kääb S, Brugada P, Robyns T, Giachino DF, Ackerman MJ, Brugada R, Brugada-Terradellas J, Gimeno JR, Hasdemir C, Guicheney P, Priori SG, Schulze-Bahr E, Makita N, Schwartz PJ, Shimizu W, Aiba T, Schott JJ, Redon R, Ohno S, Probst V, Behr ER, Barc J, Bezzina CR, and Nantes Referral Center for inherited cardiac arrhythmia
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congenital, hereditary, and neonatal diseases and abnormalities ,LQTS ,variant interpretation ,cardiovascular diseases ,Brugada ,ACMG/AMP guidelines - Abstract
PURPOSE: Stringent variant interpretation guidelines can lead to high rates of variants of uncertain significance (VUS) for genetically heterogeneous disease like long QT syndrome (LQTS) and Brugada syndrome (BrS). Quantitative and disease-specific customization of American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines can address this false negative rate. METHODS: We compared rare variant frequencies from 1847 LQTS (KCNQ1/KCNH2/SCN5A) and 3335 BrS (SCN5A) cases from the International LQTS/BrS Genetics Consortia to population-specific gnomAD data and developed disease-specific criteria for ACMG/AMP evidence classes-rarity (PM2/BS1 rules) and case enrichment of individual (PS4) and domain-specific (PM1) variants. RESULTS: Rare SCN5A variant prevalence differed between European (20.8%) and Japanese (8.9%) BrS patients (p = 5.7 × 10(-18)) and diagnosis with spontaneous (28.7%) versus induced (15.8%) Brugada type 1 electrocardiogram (ECG) (p = 1.3 × 10(-13)). Ion channel transmembrane regions and specific N-terminus (KCNH2) and C-terminus (KCNQ1/KCNH2) domains were characterized by high enrichment of case variants and >95% probability of pathogenicity. Applying the customized rules, 17.4% of European BrS and 74.8% of European LQTS cases had (likely) pathogenic variants, compared with estimated diagnostic yields (case excess over gnomAD) of 19.2%/82.1%, reducing VUS prevalence to close to background rare variant frequency. CONCLUSION: Large case-control data sets enable quantitative implementation of ACMG/AMP guidelines and increased sensitivity for inherited arrhythmia genetic testing.
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- 2021
3. Search for NΔ Resonance State Via the Exclusive Measurement of γd→dπ+π- Reaction.
- Author
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Toyama, Y., Ishikawa, T., Kanda, H., Kaneta, M., Maeda, K., Muroi, Y., Nagao, S., and Nakamura, S. N.
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MAGNETIC spectrometer , *MASS spectrometry , *RESONANCE , *DEUTERIUM , *PHOTONS , *RESEARCH institutes - Abstract
We investigate the γ d → d π + π - reaction for a study of a possible N Δ dibaryon state using a deuterium target with a magnetic spectrometer, Neutral Kaon Spectrometer 2 (NKS2), at Research Center for Electron Photon Science (ELPH), Tohoku University. The incident photon energy ranged from 0.78 to 1.08 GeV. Resonance-like structures were found at a mass of ∼ 2.15 GeV / c 2 with a width of ∼ 0.1 GeV / c 2 in the d π + and d π - invariant mass spectra. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Investigation on the rudder force of a ship in large drifting conditions with the MMG model.
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Yasukawa, H., Ishikawa, T., and Yoshimura, Y.
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STEERING gear , *SHIP models , *ABSOLUTE value , *SHIPS - Abstract
The rudder force, including interference with a ship hull in large drifting conditions, is investigated in this study by captive model tests using a ship model with steering. In the tests, hydrodynamic forces acting on the ship, the rudder normal force, and propeller thrust are measured while changing the hull drift angle β in the range of - 180 ∘ ∼ 180 ∘ , which encompasses forward, lateral, and astern motion. The hull and rudder interaction coefficients, thrust deduction factor, inflow velocity to propeller, and inflow velocity to rudder are obtained in large drifting conditions using the measured hydrodynamic force data. The Maneuvering Model Group (MMG) model (Yasukawa and Yoshimura, 2015) is applicable for estimating the rudder force when the absolute value of β is smaller than 45 ∘ . In this region, the hydrodynamic force parameters for the inflow velocity to the rudder are nearly constant for any hull drift angle. However, in the region where the absolute value of β is greater than nearly 45 ∘ , the MMG model cannot be applied. For improving the formula for the longitudinal inflow velocity component to the rudder ( u R ), we propose a new formula that is applicable to any hull drift angle. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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5. Safety and efficacy of PD-1/PD-L1 blockade in patients with preexisting antinuclear antibodies.
- Author
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Sakakida, T., Ishikawa, T., Chihara, Y., Harita, S., Uchino, J., Tabuchi, Y., Komori, S., Asai, J., Narukawa, T., Arai, A., Tsunezuka, H., Kosuga, T., Konishi, H., Moriguchi, M., Yasuda, H., Hongo, F., Inoue, M., Hirano, S., Ukimura, O., and Itoh, Y.
- Abstract
Purpose: Immune checkpoint inhibitors (ICIs) show promising clinical activity in advanced cancers. However, the safety and efficacy of PD-1/PD-L1 blockade in patients with preexisting antinuclear antibodies (ANA) are unclear. Methods: 191 patients treated with nivolumab, pembrolizumab, atezolizumab, or durvalumab for unresectable advanced cancers between September 2014 and December 2018 were identified retrospectively. Patients were divided into positive (ANA titers ≥ 1:160) and negative ANA groups (ANA titers < 1:160). Development of immune-related adverse events (irAEs), the overall response rate (ORR), and disease control rate (DCR) were monitored. Results: Positive ANA titers were seen in 9 out of 191 patients. Four patients in the positive ANA group and 69 patients in the negative group developed irAEs of any grade without a significant difference between the groups. The development of endocrine, pulmonary, and cutaneous irAEs was not significant, whereas positive ANA was significantly higher in patients who developed colitis (2/9) than in patients who did not (3/182, P = 0.0002). DCR in the positive and negative ANA group was 37.5% and 67.5%, respectively, and was not statistically significant, but had better efficacy in patients without ANA (P = 0.08). ANA-related autoimmune diseases such as SLE, Sjögren's syndrome, MCTD, scleroderma, dermatomyositis, and polymyositis was not induced in either group. However, one patient with preexisting dermatomyositis had a flare up after initiation of atezolizumab. Conclusion: Further studies to identify predictive factors for the development of irAEs are required to provide relevant patient care and maximize the therapeutic benefits of ICIs. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Investigating the Application of Customized Abrasive Cutoff Wheels with Respect to Tool Wear and Subsurface Integrity in Metallographic Cutting of Pure Titanium.
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Braz, C. O., Ventura, C. E. H., de Oliveira, M. C. B., Antonialli, A. Í. S., and Ishikawa, T.
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- 2019
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7. Nanofocusing of X-ray free-electron laser using wavefront-corrected multilayer focusing mirrors.
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Matsuyama, S., Inoue, T., Yamada, J., Kim, J., Yumoto, H., Inubushi, Y., Osaka, T., Inoue, I., Koyama, T., Tono, K., Ohashi, H., Yabashi, M., Ishikawa, T., and Yamauchi, K.
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- 2018
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8. Patch Plate Repair Method for Steel Structures Combining Adhesives and Stud Bolts.
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Ishikawa, T. and Ikeda, T.
- Abstract
Recently, a repair method by bonding patch plates is being applied to corroded steel structures. However, one of the issues of patch plate bonding repair is the brittle debonding of the patch plates. Generally, when the delamination of the patch plates occurs, the composite effect acting between the steel members and patch plates abruptly decreases. Therefore, to prevent the brittle debonding of the patch plates, a repair method combining an adhesive and stud bolts is proposed. Till date, tensile and compressive tests have been performed for the proposed method. In this study, plate bending tests were conducted to verify the effectiveness of this method under bending conditions. Furthermore, two types of epoxy resin-based adhesives were prepared to evaluate the effectiveness of the proposed method with different adhesive properties. The test results show that the proposed method is able to prevent the brittle debonding of the patch plates in the case of both epoxy resins. [ABSTRACT FROM AUTHOR]
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- 2018
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9. Redox Balance in Chloroplasts as a Modulator of Environmental Stress Responses: The Role of Ascorbate Peroxidase and Nudix Hydrolase in Arabidopsis.
- Author
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Ishikawa, T., Maruta, T., Ogawa, T., Yoshimura, K., and Shigeoka, S.
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- 2016
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10. Texture Evolutions in Annealing Process for Medium Manganese Steel Sheets.
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Okuda, K., Ishikawa, T., Omori, T., and Kainuma, R.
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- 2016
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11. Thermal stress measurement in steel plate strengthened by CFRP and aluminum alloy plates.
- Author
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Ishikawa, T., Kawano, H., Nagao, T., and Kobayashi, A.
- Abstract
In steel members strengthened by carbon-fiber reinforced polymer (CFRP) plates, the thermal stresses are introduced in the steel members, the CFRP plates and the adhesive layers when temperature changes because the linear thermal expansion coefficients of steel and CFRP are mismatched. As so far, the authors proposed a technique to reduce the thermal stress in steel members strengthened by CFRP plates, which involves bonding aluminum alloy plates with CFRP plates. In the proposed method, the thermal stress in steel member can be reduced so that there are negligible levels of stress in steel member when the cross sectional areas of CFRP and aluminum plates are designed to correspond to the coefficient of thermal expansion of steel, even though the thermal stresses are introduced in the CFRP and aluminum plates. In this study, to confirm the maintaining the thermal stress reduction in steel member by proposed method, thermal stress measurement in steel plate strengthened by CFRP and aluminum plates was carried out about 21 months. In this research, the thermal stress introduced in the steel plate strengthened by CFRP plates was also measured. Furthermore, to assume the thermal shear and normal (peel) stresses in adhesive layers, FE analysis with plane stress element was employed. As the result, it was shown the thermal stresses in steel plate with CFRP plate were able to calculate by using composite theory and measured temperature. Furthermore, in steel plate strengthened by CFRP and aluminum plates, the thermal stress introduced in steel plate was negligible-small through the all-season. It was found the thermal stresses in steel plate with CFRP plates as well as CFRP and aluminum plates were also estimated by using composite theory and measured temperature. In the steel plate strengthened by CFRP and aluminum plates, the thermal shear and normal stresses in adhesive layer glued to steel plate become smaller than that in the conventional CFRP bonded specimen. However, the shear stress in adhesive layers between CFRP and aluminum plates in proposed method was higher than the thermal stress in adhesive layers between CFRP plates in conventional method. [ABSTRACT FROM AUTHOR]
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- 2016
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12. 10 fs Dynamics of Photoinduced Magnetic Transition in Double-Layered Charge Ordering in LuFe2O4 Under Interlayer Excitation.
- Author
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Sagae, Y., Yamada, K., Ishikawa, T., Itoh, K., Itoh, H., Sasaki, T., Nagata, T., Kano, J., Kambe, T., Ishihara, S., Ikeda, N., and Iwai, S.
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- 2015
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13. Spontaneous Formation of Correlated Charge Coherence Induced by 1.5-Cycle Pulse in 1-D Organic Metal (TMTTF)2AsF6.
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Ishikawa, T., Sagae, Y., Naito, Y., Ichimura, J., Kawakami, Y., Itoh, H., Yamamoto, K., Yakushi, K., Ishihara, S., Sasaki, T., Yonemitsu, K., and Iwai, S.
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- 2015
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14. Determination of Absolute Cross-Sections of Nonresonant EUV-UV Two-Color Two-Photon Ionization of He.
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Fushitani, M., Hikosaka, Y., Matsuda, A., Endo, T., Shigemasa, E., Nagasono, M., Sato, T., Togashi, T., Yabashi, M., Ishikawa, T., and Hishikawa, A.
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- 2015
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15. Evaluation of genetic characteristics of wild and cultured populations of the Japanese pearl oyster Pinctada fucata martensii by using AFLP markers.
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Miyake, T., Isowa, K., Ishikawa, T., Komaru, A., and Kawamura, K.
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PINCTADA ,PEARL oyster culture ,AMPLIFIED fragment length polymorphism ,GENETIC markers ,BREEDING - Abstract
Genetic characteristics of four wild (Mie, Fukui, Shimane and Nagasaki) and five cultured populations (selectively bred for 12 years with an origin of Ehime population) of the Japanese pearl oyster, Pinctada fucata martensii, were evaluated by using AFLP markers. Six primer pairs generated 1019 loci in total, among which 45.2-55.1 % was polymorphic among populations. Although there was no significant difference in gene diversity between wild (0.170-0.174) and cultured (0.158-0.173) populations, genetic relatedness in cultured populations (0.316-0.450) was about three times higher than that in wild populations (0.110-0.165). In addition, genetic differentiation was about twenty times larger in cultured populations (Nei's distance: 0.0111) than in wild populations (Nei's distance: 0.0005). These results mean that selective breeding can cause marked inbreeding as well as large genetic differentiation among cultured populations in a short period. On the other hand, it was suggested that genetic homogenization in the wild, probably due to a large-scale transport of cultured oysters, had progressed in the sea around Japan. In conclusion, it is necessary to prevent inbreeding by the reconsideration of the style of selective breeding in cultured populations, while the escape of gametes or spats of cultured strains in the wild should be avoided for the preservation of genetic characteristics in native populations of P. f. martensii. [ABSTRACT FROM AUTHOR]
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- 2016
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16. Relationships between capture-site characteristics and capture levels of the invasive mongoose on Amami-Oshima Island, Japan.
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Komine, H., Takeshita, K., Abe, S., Ishikawa, T., Kimura, M., Hashimoto, T., Kitaura, K., Morosawa, T., Seki, K., and Kaji, K.
- Abstract
We used a generalized linear model (GLM) to investigate environmental characteristics of capture sites of the invasive small Indian mongoose ( Herpestes auropunctatus) on Amami Island, Japan. Data were collected from 2002 to 2011, from an area with the lowest mongoose density. Data on capture levels and environmental factors were obtained within 1 × 1 km grid cells. In addition, we developed an evaluation map that predicts the occurrence of mongooses throughout the island by extrapolating the 2011 results, in which the last mongoose appearance was confirmed. We found that environmental characteristics of capture sites drastically changed during the final stage of the eradication project. From 2002 to 2010, mongooses were captured in grid cells with many gentle ridges. In 2011, when mongoose density became exceedingly low, mongooses seem to have survived in the grid cells with abundant steep ridges, where it was difficult to establish traps. Therefore, environmental factors affected capture levels and the density of the mongoose population. The evaluation map predicts that mongooses could survive on the southwest and southeast island peninsulas. This evaluation map will be helpful in allocating limited labor and financial resources toward the most effective capture strategies. This study suggests that an analysis of pest management data is an important step in the eradication of mongooses. Our results can contribute to long-term projects in invasive pest management not only for the mongoose population on Amami Island, but also for other invasive species globally. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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17. Femtosecond two-photon Rabi oscillations in excited He driven by ultrashort intense laser fields.
- Author
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Fushitani, M., Liu, C.-N., Matsuda, A., Endo, T., Toida, Y., Nagasono, M., Togashi, T., Yabashi, M., Ishikawa, T., Hikosaka, Y., Morishita, T., and Hishikawa, A.
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- 2016
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18. Magneto-optical trapping of radioactive atoms for test of the fundamental symmetries.
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Kawamura, Hirokazu, Ando, S., Aoki, T., Arikawa, H., Harada, K., Hayamizu, T., Inoue, T., Ishikawa, T., Itoh, M., Kato, K., Köhler, L., Mathis, J., Sakamoto, K., Uchiyama, A., and Sakemi, Y.
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ION traps ,MAGNETOOPTICS ,FRANCIUM ,SYMMETRY (Physics) ,STANDARD model (Nuclear physics) - Abstract
We are planning test experiments of fundamental symmetries based on the intrinsic properties of francium. It is expected that the laser cooling and trapping of francium will produce precision measurements. The pilot experiment using rubidium was performed with the goal of francium trapping. The ion beam generated with a francium ion source was investigated using a Wien filter. Each piece of equipment still must be studied in more detail, and the equipment should be upgraded in order to trap radioactive atoms. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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19. Gastric Lymph Node Cancer Detection Using Multiple Features Support Vector Machine for Pathology Diagnosis Support System.
- Author
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Ishikawa, T., Takahashi, J., Takemura, Hiroshi, Mizoguchi, Hiroshi, and Kuwata, T.
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- 2014
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20. Experimental search for the electron electric dipole moment with laser cooled francium atoms.
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Inoue, T., Ando, S., Aoki, T., Arikawa, H., Ezure, S., Harada, K., Hayamizu, T., Ishikawa, T., Itoh, M., Kato, K., Kawamura, H., Uchiyama, A., Asahi, K., Furukawa, T., Hatakeyama, A., Hatanaka, K., Imai, K., Murakami, T., Nataraj, H., and Sato, T.
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ELECTRIC dipole moments ,FRANCIUM ,RADIOISOTOPES ,CYCLOTRONS ,LASER cooling - Abstract
A laser cooled heavy atom is one of the candidates to search for the permanent electric dipole moment (EDM) of the electron due to the enhancement mechanism and its long coherence time. The laser cooled francium (Fr) factory has been constructed to perform the electron EDM search at the Cyclotron and Radioisotope Center, Tohoku University. The present status of Fr production and the EDM measurement system is presented. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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21. Microscale Flow Dynamics of Red Blood Cells in Microchannels: An Experimental and Numerical Analysis.
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Lima, R., Fernandes, C. S., Dias, R., Ishikawa, T., Imai, Y., and Yamaguchi, T.
- Abstract
The blood flow dynamics in microcirculation depends strongly on the microvascular networks composed with short irregular vessel segments which are linked by numerous bifurcations. This paper presents the application of a confocal micro-PTV system to track RBCs through a rectangular polydimethysiloxane (PDMS) microchannel with a bifurcation. By using a confocal micro-PTV system, we have measured the effect of bifurcation on the flow behaviour of both fluorescent particles diluted in pure water and RBCs in concentrated suspensions. After performing simulations with the commercial finite element software package POLYFLOW
®; , some experimental results were compared with the numerical results and the limitations of these simulations were outlined. [ABSTRACT FROM AUTHOR]- Published
- 2011
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22. Microscale Flow Dynamics of Red Blood Cells in Microchannels: An Experimental and Numerical Analysis.
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Lima, R., Nakamura, M., Omori, T., Ishikawa, T., Wada, S., and Yamaguchi, T.
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The blood flow dynamics in microcirculation depends strongly on the motion, deformation and interaction of red blood cells (RBCs) within the microves-sel. We present confocal micro-PTV measurements on the motion of individual RBCs through a circular polydimethysiloxane (PDMS) microchannel. The RBC radial displacement and dispersion calculated from these measurements show that the RBC paths are strongly dependent on the both Hct and plasma layer. In order to obtain more detailed information of the non-Newtonian property of blood a novel computational scheme is also described. The simulated flow dynamics were in good agreement with the Casson flow model and in vivo observations. In the near future by comparing both results we hope to clarify a variety of complex phenomena occurring at the microscale level. [ABSTRACT FROM AUTHOR]
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- 2009
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23. Precision spectroscopy of kaonic helium-4 X-rays.
- Author
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Hayano, R. S., Beer, G., Bhang, H., Cargnelli, M., Chiba, J., Choi, S., Curceanu, C., Fukuda, Y., Hanaki, T., Iio, M., Ishikawa, T., Ishimoto, S., Ishiwatari, T., Itahashi, K., Iwai, M., Iwasaki, M., Juhász, B., Kienle, P., Marton, J., and Matsuda, Y.
- Abstract
The Balmer-series X-rays of kaonic helium-4 have been re-measured with a projected goal of determining the 2p-level strong-interaction shift to a precision better than a few electron volts. Preliminary results indicate that the shift must be much smaller than the current world average of −43 ± 8 eV. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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24. Search for a kaonic nuclear state via 4He(K−, N) reaction at rest.
- Author
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Iwasaki, M., Bhang, H., Chiba, J., Choi, S., Fukuda, Y., Hanaki, T., Hayano, R. S., Iio, M., Ishikawa, T., Ishimoto, S., Ishiwatari, T., Itahashi, K., Iwai, M., Kienle, P., Kim, J. H., Matsuda, Y., Ohnishi, H., Okada, S., Outa, H., and Sato, M.
- Abstract
Very recently, we have performed a couple of experiments, KEK PS-E549/E570, for the detailed study of the strange tribaryon S
0 (3115) obtained in KEK PS-E471. These experiments were performed to accumulate much higher statistics with improved experimental apparatus especially for the better proton spectroscopy of the4 He(stopped K− , N) reaction. In contrast to the previous proton spectrum, no narrow (∼ 20 MeV) peak structure was found either in the inclusive4 He(stopped K− , p) or in the semi-inclusive4 He(stopped K− , pX± ) reaction channel, which is equivalent to the previous E471 event trigger condition. Detailed analysis of the present data and simulation shows that the peak, corresponding to S0 (3115), has been an experimental artifact. Present analysis does not exclude the possible existence of a much wider structure. To be sensitive to such structure and for better understanding of the non-mesonic K− absorption reaction channel, detailed analysis of the data is in progress. [ABSTRACT FROM AUTHOR]- Published
- 2007
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25. Experimental search of strange tribaryons in the 4He(K stop − , n) reaction.
- Author
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Yim, H., Bhang, H., Chiba, J., Choi, S., Fukuda, Y., Hanaki, T., Hayano, R. S., Iio, M., Ishikawa, T., Ishimoto, S., Ishiwatari, T., Itahashi, K., Iwai, M., Iwasaki, M., Kienle, P., Kim, J. H., Matsuda, Y., Ohnishi, H., Okada, S., and Outa, H.
- Abstract
Recent prediction of Akaishi and Yamazaki on the strongly bound kaonic nuclear states with remarkably narrow widths have generated a lot of physics interests and the states have been studied and discussed intensively [1]. The fundamental motivation behind such intense study was that on one hand they could give us the excellent opportunities to investigate dense and cold hadronic systems in which quarks may be deconfined and the partial restoration of the chiral symmetry is expected to occur in a large scale [2], on the other hand the existence itself with such narrow width is extremely peculiar and interesting. Since then, the states have been studied in a series of experiments at BNL [3], KEK [4,5], GSI [6] and LNF [7]. We have recently reported an indication of a strange tribaryon state, S+, with a width Γ < 23 MeV/c
2 , in the neutron time of flight (TOF) spectrum of the4 He(Kstop − , n) reaction in the KEK-PS E471 which was the first experiment at KEK-PS to search for the deeply bound narrow K− ppn (T=0) state [4]. In order to confirm the state, S+ , and search for other possible total isospin T=0/1 tribaryonic states, we have re-measured the neutron energy spectrum from4 He(Kstop − , n) reaction in the KEK-PS E549 experiment with the upgraded setup with which TOF resolution was improved 1.5 times and the statistics was increased 6 times. However, in the neutron spectra, we find such a smooth momentum distribution that we conclude the state either not so strong to stick out of the inclusive background or too broad to form a peak. It is now necessary to analyze the data for further details such as the hyperon motion correlation. [ABSTRACT FROM AUTHOR]- Published
- 2007
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26. Sensitive Depiction of Biomedical Tissue by Refraction Based Computed Tomography Using Synchrotron Radiation.
- Author
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Kim, Sun I., Suh, Tae Suk, Magjarevic, R., Nagel, J. H., Hashimoto, Eiko, Maksimenko, A., Sugiyama, H., Hirano, Keiichi, Hyodo, K., Shimao, D., Nishino, Y., Ishikawa, T., Yuasa, T., and Ando, M.
- Abstract
We applied the recently developed X-ray refraction based computed tomography to a biomedical object. The algorithm used in this study to reconstruct CT images has been invented in our group. The X-ray data was acquired by DEI (diffraction enhanced imaging) which comprises a double crystal arrangement of highly perfect silicon crystals. The ability of Refraction Based contrast CT to depict lung disease was tested by observing human lung cancer with synchrotron X-rays. Reconstructed images of lung cancer show much detail than absorption-based CT. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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27. Person Tracking and Multicamera Video Retrieval Using Floor Sensors in a Ubiquitous Environment.
- Author
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Wee-Kheng Leow, Lew, Michael S., Tat-Seng Chua, Wei-Ying Ma, Chaisorn, Lekha, Bakker, Erwin M., Silva, Gamhewage C., Ishikawa, T., Yamasaki, T., and Aizawa, K.
- Abstract
A system for video retrieval from a ubiquitous environment is presented. Data from pressure-based floor sensors are used as a supplementary input for retrieving video from a large number of cameras. An algorithm based on agglomerative hierarchical clustering is used to segment footpaths of individual persons. Video handover is proposed and two methods are implemented to retrieve video clips and key frame sequences showing a person moving inside the house. The video clips are further segmented according to the actions performed. We evaluate the performance of each stage of retrieval and present the results. The paper concludes with suggestions for improvements, and future directions. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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28. TNF-α/TNFR1 signaling promotes gastric tumorigenesis through induction of Noxo1 and Gna14 in tumor cells.
- Author
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Oshima, H, Ishikawa, T, Yoshida, G J, Naoi, K, Maeda, Y, Naka, K, Ju, X, Yamada, Y, Minamoto, T, Mukaida, N, Saya, H, and Oshima, M
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STOMACH cancer treatment , *TUMOR necrosis factor receptors , *CELLULAR signal transduction , *NADPH oxidase , *G proteins , *CANCER cells , *HELICOBACTER pylori , *BACTERIAL diseases - Abstract
Helicobacter pylori infection induces chronic inflammation that contributes to gastric tumorigenesis. Tumor necrosis factor (TNF-α) is a proinflammatory cytokine, and polymorphism in the TNF-α gene increases the risk of gastric cancer. We herein investigated the role of TNF-α in gastric tumorigenesis using Gan mouse model, which recapitulates human gastric cancer development. We crossed Gan mice with TNF-α (Tnf) or TNF-α receptor TNFR1 (Tnfrsf1a) knockout mice to generate Tnf−/− Gan and Tnfrsf1a−/− Gan mice, respectively, and examined their tumor phenotypes. Notably, both Tnf−/− Gan mice and Tnfrsf1a−/− Gan mice showed similar, significant suppression of gastric tumor growth compared with control Tnf+/+ or Tnfrsf1a+/+ Gan mice. These results indicate that TNF-α signaling through TNFR1 is important for gastric tumor development. Bone marrow (BM) transplantation experiments showed that TNF-α expressed by BM-derived cells (BMDCs) stimulates the TNFR1 on BMDCs by an autocrine or paracrine manner, which is important for gastric tumor promotion. Moreover, the microarray analysis and colony formation assay indicated that NADPH oxidase organizer 1 (Noxo1) and Gna14 are induced in tumor epithelial cells in a TNF-α-dependent manner, and have an important role in tumorigenicity and tumor-initiating cell property of gastric cancer cells. Accordingly, it is possible that the activation of TNF-α/TNFR1 signaling in the tumor microenvironment promotes gastric tumor development through induction of Noxo1 and Gna14, which contribute to maintaining the tumor cells in an undifferentiated state. The present results indicate that targeting the TNF-α/TNFR1 pathway may be an effective preventive or therapeutic strategy for gastric cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
29. Prognostic significance of CD44 variant 2 upregulation in colorectal cancer.
- Author
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Ozawa, M, Ichikawa, Y, Zheng, Y-W, Oshima, T, Miyata, H, Nakazawa, K, Guan, H-B, Shiozawa, M, Akaike, M, Watanabe, K, Ota, M, Fujii, S, Kunisaki, C, Ishikawa, T, Tanaka, K, Akiyama, H, Endo, I, and Taniguchi, H
- Subjects
COLON cancer ,CANCER stem cells ,TUMOR markers ,FLOW cytometry ,CELL populations ,XENOGRAFTS - Abstract
Background:CD133 and CD44 are putative cancer stem cell (CSC) markers in colorectal cancer (CRC). However, their clinical significance is currently unclear. Here, we evaluated primary CRC cell isolates to determine the significance of several CSC markers, including CD133 and CD44, as predictors of tumourigenesis and prognosis.Methods:CD133- and CD44-positive cells from fresh clinical samples of 77 CRCs were selected by flow cytometric sorting and evaluated for tumourigenicity following subcutaneous transplantation into NOD/SCID mice. Cancer stem cell marker expression was examined in both xenografts and a complementary DNA library compiled from 167 CRC patient samples.Results:CD44
+ , CD133+ and CD133+ CD44+ sub-populations were significantly more tumourigenic than the total cell population. The clinical samples expressed several transcript variants of CD44. Variant 2 was specifically overexpressed in both primary tumours and xenografts in comparison with the normal mucosa. A prognostic assay using qRT-PCR showed that the CD44v2high group (n=84, 5-year survival rate (5-OS): 0.74) had a significantly worse prognosis (P=0.041) than the CD44v2low group (n=83, 5-OS: 0.88).Conclusions:CD44 is an important CSC marker in CRC patients. Furthermore, CRC patients with high expression of CD44v2 have a poorer prognosis than patients with other CD44 variants. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
30. Viscosity of Molten InSb, GaSb, and $$\mathrm{{In}}_{x}\mathrm{{Ga}}_{1-{x}}\mathrm{{Sb}}$$ Alloy Semiconductors.
- Author
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Sakata, K., Mukai, M., Rajesh, G., Arivanandhan, M., Inatomi, Y., Ishikawa, T., and Hayakawa, Y.
- Subjects
VISCOSITY ,INDIUM antimonide ,GALLIUM antimonide ,SEMICONDUCTORS ,VISCOSIMETERS - Abstract
The viscosities of molten InSb, GaSb, and $$\mathrm{{In}}_{{x}}\mathrm{{Ga}}_{1-{x}}\mathrm{{Sb}}$$ ( $$x=0.2$$ and 0.4) were measured as a function of temperature using an oscillating viscometer for fundamental understanding of the physical properties for fabricating high quality InGaSb multicomponent semiconductor crystals. The measured values showed good Arrhenius linearity for InSb, GaSb, and $$\mathrm{{In}}_{{x}}\mathrm{{Ga}}_{1-{x}}\mathrm{{Sb}}$$ samples. The absolute values of the viscosity for InSb and GaSb agreed with a previous study. Also, it is suggested that the absolute values of the viscosity among the compounds are quite similar, and the results can be associated with their crystal structures. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
31. Improvement of GPS/acoustic seafloor positioning precision through controlling the ship's track line.
- Author
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Sato, M., Fujita, M., Matsumoto, Y., Saito, H., Ishikawa, T., and Asakura, T.
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SUBMARINE topography ,GLOBAL Positioning System ,SATELLITE-based remote sensing ,SUBMARINE trenches - Abstract
The precision of GPS/acoustic seafloor positioning was improved by introducing a hull-mounted onboard system in March 2008, which allows us to conduct acoustic ranging measurements with the vessel sailing along the pre-determined track lines, while the early system before 2008 could only adopt the uncontrollable drifting observation. The continuity of the positioning results due to the transition was first confirmed through the comparison between results from sailing and drifting observations conducted in parallel. Using the data acquired for about 3 years since 2008, the repeatability of the determined position for the sailing observation was evaluated to be about 2 cm in root mean squares in the horizontal component, significantly better than that for the early drifting observation. The improvement of positioning precision probably resulted from the improvement of geometric distribution of acoustic ranging data by controlling the track lines. It was also shown that the sailing observation allows to obtain reliable results with a smaller amount of data. Comparison between the results in different sea regions suggests that positioning precision is better in the region along the Nankai Trough than in the region along the Japan Trench, probably because of the complicated acoustic velocity structure of seawater often observed in the latter. Furthermore, the precision of height determination was also improved, which leads us to expect that vertical crustal movement will be detectable in the future through accumulation of data as well as further technology development. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
32. π and η Photoproduction on the Deuteron at ELPH, Tohoku University.
- Author
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Ishikawa, T., Fujimura, H., Fukasawa, H., Hashimoto, R., He, Q., Honda, Y., Iwata, T., Kaida, S., Kasagi, J., Kawano, A., Kuwasaki, S., Maeda, K., Masumoto, S., Miyabe, M., Miyahara, F., Mochizuki, K., Muramatsu, N., Nakamura, A., Nawa, K., and Ogushi, S.
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DEUTERONS , *QUANTUM chromodynamics , *PHYSICS experiments , *ELECTROMAGNETISM , *NUCLEAR cross sections ,BARYON spectra - Abstract
Baryon spectroscopy is important to understand Quantum Chromodynamics at low energies. In this purpose, a series of π and η photoproduction experiments was carried out with an electro-magnetic calorimeter FOREST at Research Center for Electron Photon Science, Tohoku University. The incident tagged bremsstrahlung photon energy ranges from 550 to 1,150 MeV. The differential and total cross sections obtained for π and η photoproduction processes on the proton are consistent with the SAID and MAID calculations. The analysis of π and η photoproduction on the neutron is underway. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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- View/download PDF
33. Study for the ω- N Interaction Through ω Photoproduction Near the Threshold.
- Author
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Hashimoto, R., Fujimura, H., Fukasawa, H., He, Q., Honda, Y., Ishikawa, T., Iwata, T., Kaida, S., Kasagi, J., Kawano, A., Kuwasaki, S., Maeda, K., Masumoto, S., Miyabe, M., Miyahara, F., Mochizuki, K., Muramatsu, N., Nakamura, A., Nawa, K., and Ogushi, S.
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THRESHOLD voltage ,NUCLEAR cross sections ,MESONS ,CENTER of mass ,SET theory ,SCATTERING (Physics) ,PHYSICS experiments - Abstract
The total cross section has been measured for ω meson photoproduction on the proton for the center-of-mass energy $${\sqrt{s}}$$ ranging from 1.69 to 1.75 GeV. Including the subthreshold region of ω photoproduction, a set of about 40 data points has been obtained with a fine energy step of 1-2 MeV. The data set enables us to deduce the ω- N scattering length. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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- View/download PDF
34. Study of γ d → π π d Reaction in an Energy Region of 0.67 ≤ E ≤ 1.08 GeV.
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Kimura, C., Beckford, B., Fujibayashi, T., Fujii, T., Fujii, Y., Futatsukawa, K., Gogami, T., Hashimoto, O., Han, Y., Hirose, K., Honda, R., Hosomi, K., Iguchi, A., Ishikawa, T., Kanda, H., Kaneko, Y., Kaneta, M., Kasai, Y., Kawasaki, T., and Kiyokawa, S.
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NUCLEAR reactions ,DEUTERONS ,RESEARCH institutes ,PHOTON beams ,NEUTRAL kaon ,SPECTROMETERS - Abstract
Double charged pion photoproductions from the deuteron have been studied at Research Center for Electron Photon Science (ELPH), Tohoku University. Tagged photon beams in an energy range of 0.67 ≤ E ≤ 1.08 GeV were impinged on the liquid deuteron target. Produced charged particles were observed with the Neutral Kaon Spectrometer2 (NKS2). The γ d → π π d process was clearly separated among the three charged tracks. We obtained the photon energy dependence of the normalized yields of this process. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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- View/download PDF
35. Direct binding of Grb2 has an important role in the development of myeloproliferative disease induced by ETV6/FLT3.
- Author
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Chonabayashi, K, Hishizawa, M, Kawamata, S, Nagai, Y, Ohno, T, Ishikawa, T, Uchiyama, T, and Takaori-Kondo, A
- Subjects
CARRIER protein genetics ,MYELOPROLIFERATIVE neoplasms ,GENETICS - Abstract
A letter to the editor is presented in response to the article on the significance of the growth factor receptor-binding protein 2 (Grb2) in myeloproliferative disease development.
- Published
- 2013
- Full Text
- View/download PDF
36. Comparison of outcomes between autologous and allogeneic hematopoietic stem cell transplantation for peripheral T-cell lymphomas with central review of pathology.
- Author
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Kim, S-W, Yoon, S-S, Suzuki, R, Matsuno, Y, Yi, H G, Yoshida, T, Imamura, M, Wake, A, Miura, K, Hino, M, Ishikawa, T, Kim, J S, Maeda, Y, Lee, J-J, Kang, H J, Lee, H S, Lee, J-H, Izutsu, K, Fukuda, T, and Kim, C W
- Subjects
HEMATOPOIETIC stem cell transplantation ,T-cell lymphoma ,THERAPEUTICS - Abstract
A letter to the editor is presented in response to the article on the outcomes of allogeneic and autologous hematopoietic stem cell transplantation for peripheral T-cell lymphomas with central review of pathology.
- Published
- 2013
- Full Text
- View/download PDF
37. Biological nitrification inhibition (BNI) activity in sorghum and its characterization.
- Author
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Subbarao, G., Nakahara, K., Ishikawa, T., Ono, H., Yoshida, M., Yoshihashi, T., Zhu, Yiyong, Zakir, H., Deshpande, S., Hash, C., and Sahrawat, K.
- Subjects
NITRIFICATION inhibitors ,NITRIFICATION ,SORGHUM ,NITRATES ,SOIL composition ,CHROMATOGRAPHIC analysis ,CHEMICAL structure ,HYDROPHILIC compounds ,NITROSOMONAS europaea - Abstract
Aims: The ability to suppress soil nitrification through the release of nitrification inhibitors from plant roots is termed 'biological nitrification inhibition' (BNI). Here, we aimed at the quantification and characterization of the BNI function in sorghum that includes inhibitor production, their chemical identity, functionality and factors regulating their release. Methods: Sorghum was grown in solution culture and root exudate was collected using aerated NHCl solutions. A bioluminescence assay using recombinant Nitrosomonas europaea was employed to determine the BNI activity. Activity-guided chromatographic fractionation was used to isolate biological nitrification inhibitors (BNIs). The chemical structure was analyzed using NMR and mass spectrometry; pH-stat systems were deployed to analyze the role of rhizosphere pH on BNIs release. Results: Sorghum roots released two categories of BNIs: hydrophilic- and hydrophobic-BNIs. The release rates for hydrophilic- and hydrophobic- BNIs ranged from 10 to 25 ATU g root dwt. d. Addition of hydrophilic BNIs (10 ATU g soil) significantly inhibited soil nitrification (40 % inhibition) during a 30-d incubation test. Two BNI compounds isolated are: sakuranetin (ED 0.6 μM; isolated from hydrophilic-BNIs fraction) and sorgoleone (ED 13.0 μM; isolated from hydrophobic-BNIs fraction), which inhibited Nitrosomonas by blocking AMO and HAO enzymatic pathways. The BNIs release required the presence of NH in the root environment and the stimulatory effect of NH lasted 24 h. Unlike the hydrophobic-BNIs, the release of hydrophilic-BNIs declined at a rhizosphere pH >5.0; nearly 80 % of hydrophilic-BNI release was suppressed at pH ≥7.0. The released hydrophilic-BNIs were functionally stable within a pH range of 5.0 to 9.0. Sakuranetin showed a stronger inhibitory activity (ED 0.2 μM) than methyl 3-(4-hydroxyphenyl) propionate (MHPP) (ED 100 μM) (isolated from hydrophilic-BNIs fraction) in the in vitro culture-bioassay, but the activity was non-functional and ineffective in the soil-assay. Conclusions: There is an urgent need to identify sorghum genetic stocks with high potential to release functional-BNIs for suppressing nitrification and to improve nitrogen use efficiency in sorghum-based production systems. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
38. Full $\mathcal{O}(\alpha)$ electroweak radiative corrections to $e^{+}e^{-} \rightarrow t \bar{t} \gamma$ with GRACE-Loop.
- Author
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Khiem, P., Fujimoto, J., Ishikawa, T., Kaneko, T., Kato, K., Kurihara, Y., Shimizu, Y., Ueda, T., Vermaseren, J., and Yasui, Y.
- Subjects
ELECTROMAGNETIC interactions ,NUMERICAL analysis ,QUANTUM electrodynamics ,CENTER of mass ,LINEAR accelerators ,ELECTROWEAK interactions - Abstract
We present the full $\mathcal{O}(\alpha)$ electroweak radiative corrections to the process $e^{+}e^{-} \rightarrow t \bar{t} \gamma$ at the International Linear Collider (ILC). The computation is performed with the help of the GRACE-Loop system. We present the total cross-section and the top quark forward-backward asymmetry ( A) as a function of the center-of-mass energy and compare them with the process $e^{+}e^{-} \rightarrow t \bar{t}$. We find that the value of A in $t \bar{t} \gamma$ production is larger than A in $t\bar{t}$ production. It is an important result for the measurement of the top quark forward-backward asymmetry at the ILC. Applying a structure function method, we also subtract the QED correction to gain the genuine weak correction in both the α scheme and the G scheme ( $\delta_{W}^{G_{\mu}}$). We obtain numerical values for $\delta_{W}^{G_{\mu}}$ which are changing from 2 % to −24 % when we vary the center-of-mass energy from 360 GeV to 1 TeV. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
39. Interplay among NH uptake, rhizosphere pH and plasma membrane H-ATPase determine the release of BNIs in sorghum roots - possible mechanisms and underlying hypothesis.
- Author
-
Zhu, Yiyong, Zeng, Houqing, Shen, Qirong, Ishikawa, T., and Subbarao, Guntur
- Subjects
RHIZOSPHERE ,SORGHUM ,CELL membranes ,BIOLOGICAL assay ,ACIDIFICATION - Abstract
Aims and background: The ability to suppress soil nitrification through the release of nitrification inhibitors from plant roots is termed 'biological nitrification inhibition' (BNI). Earlier, we reported that sorghum roots release higher BNI-activity when grown with NH, but not with NO as N source. Also for BNI release, rhizosphere pH of <5.0 is needed; beyond this, a negative effect on BNI release was observed with nearly 80% loss of BNI activity at pH >7.0. This study is aimed at understanding the inter-functional relationships associated with NH uptake, rhizosphere-pH and plasma membrane H-ATPase (PM H-ATPase) activity in regulating the release of BNIs (biological nitrification inhibitors) from sorghum roots. Methods: Sorghum was grown hydroponically and root exudates were collected from intact plants using a pH-stat system to separate the secondary acidification effects by NH uptake on BNIs release. A recombinant luminescent Nitrosomonas europaea bioassay was used to determine BNI-activity. Root plasma membrane was isolated using a two-phase partitioning system. Hydrolytic H-ATPase activity was determined. Split-root system setup was deployed to understand the localized responses to NH, H-ATPase-stimulator (fusicoccin) or H-ATPase-inhibitor (vanadates) on BNI release by sorghum. Results: Presence of NH in the rhizosphere stimulated the expression of H-ATPase activity and enhanced the release of BNIs from sorghum roots. Fusicoccin, which stimulates H-ATPase activity, also stimulated BNIs release in the absence of NH; vanadate, which suppresses H-ATPase activity, also suppressed the release of BNIs. NH levels (in rhizosphere) positively influenced BNIs release and root H-ATPase activity in the concentration range of 0-1.0 mM, indicating a close relationship between BNI release and root H-ATPase activity with a possible involvement of carrier-mediated transport for the release of BNIs in sorghum. Conclusion: Our results suggest that NH uptake, PM H-ATPase activity, and rhizosphere acidification are functionally inter-connected with BNI release in sorghum. Such knowledge is critical to gain insights into why BNI function is more effective in light-textured, mildly acidic soils compared to other soil types. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
40. Inflammation-induced repression of tumor suppressor miR-7 in gastric tumor cells.
- Author
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Kong, D, Piao, Y-S, Yamashita, S, Oshima, H, Oguma, K, Fushida, S, Fujimura, T, Minamoto, T, Seno, H, Yamada, Y, Satou, K, Ushijima, T, Ishikawa, T-O, and Oshima, M
- Subjects
INFLAMMATION ,GENE expression ,TUMOR suppressor genes ,CANCER cells ,TUMOR growth ,MICRORNA ,CARCINOGENESIS ,LABORATORY mice - Abstract
Inflammation has an important role in cancer development through various mechanisms. It has been shown that dysregulation of microRNAs (miRNAs) that function as oncogenes or tumor suppressors contributes to tumorigenesis. However, the relationship between inflammation and cancer-related miRNA expression in tumorigenesis has not yet been fully understood. Using K19-C2mE and Gan mouse models that develop gastritis and gastritis-associated tumors, respectively, we found that 21 miRNAs were upregulated, and that 29 miRNAs were downregulated in gastric tumors in an inflammation-dependent manner. Among these miRNAs, the expression of miR-7, a possible tumor suppressor, significantly decreased in both gastritis and gastric tumors. Moreover, the expression of miR-7 in human gastric cancer was inversely correlated with the levels of interleukin-1β and tumor necrosis factor-α, suggesting that miR-7 downregulation is related to the severity of inflammatory responses. In the normal mouse stomach, miR-7 expression was at a basal level in undifferentiated gastric epithelial cells, and was induced during differentiation. Moreover, transfection of a miR-7 precursor into gastric cancer cells suppressed cell proliferation and soft agar colony formation. These results suggest that suppression of miR-7 expression is important for maintaining the undifferentiated status of gastric epithelial cells, and thus contributes to gastric tumorigenesis. Although epigenetic changes were not found in the CpG islands around miR-7-1 of gastritis and gastric tumor cells, we found that activated macrophage-derived small molecule(s) (<3 kDa) are responsible for miR-7 repression in gastric cancer cells. Furthermore, the miR-7 expression level significantly decreased in the inflamed gastric mucosa of Helicobacter-infected mice, whereas it increased in the stomach of germfree K19-C2mE and Gan mice wherein inflammatory responses were suppressed. Taken together, these results indicate that downregulation of tumor suppressor miR-7 is a novel mechanism by which the inflammatory response promotes gastric tumorigenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
41. Seasonal and diurnal variations of radon/thoron exhalation rate in Kanto-loam area in Japan.
- Author
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Prasad, G., Ishikawa, T., Hosoda, M., Sorimachi, A., Sahoo, S., Kavasi, N., Tokonami, S., Sugino, M., and Uchida, S.
- Subjects
- *
RADON isotopes , *SOIL temperature , *THORON , *SOIL moisture , *GAMMA rays , *RAINFALL - Abstract
Radon and thoron concentration in the outdoor environment are affected by the magnitude of the exhalation rate that can vary diurnally and seasonally. This paper presents measurement results of radon and thoron exhalation rates and gamma-ray dose rate in different season at same location points in Gunma Prefecture Japan. Exhalation rates were measured by the MSZ instrument which is based on the accumulation method. Three measurement points Katashina Village, Midori City and Takasaki City were selected for measurement. Soil water saturation and soil temperature were measured to investigate their relationship with exhalation rate. The diurnal variation of exhalation rate may be correlated with soil temperature but no clear relationship was found between them. The gamma-ray dose rate do not vary significantly at the same places even in different season. The average radon exhalation rates were 11 ± 2, 2 ± 1, 5 ± 3 and 11 ± 4 mBq m s for spring, summer, autumn and winter, respectively. Those for thoron were 1,100 ± 100, 120 ± 30, 250 ± 80 and 860 ± 140 mBq m s. Thus there was a variation of radon and thoron exhalation rate with different seasons. The radon and thoron exhalation rates in the summer and autumn surveys are higher than those in the spring and winter surveys which were affected by rainfall. It indicates that water saturation is an influential factor for radon and thoron exhalation rates. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
42. Timeline for development of autologous bone marrow infusion (ABMi) therapy and perspective for future stem cell therapy.
- Author
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Terai S, Tanimoto H, Maeda M, Zaitsu J, Hisanaga T, Iwamoto T, Fujisawa K, Mizunaga Y, Matsumoto T, Urata Y, Marumoto Y, Hidaka I, Ishikawa T, Yokoyama Y, Aoyama K, Tsuchiya M, Takami T, Omori K, Yamamoto N, and Segawa M
- Abstract
Liver cirrhosis patients generally progress to liver failure. To cure this progressive disease, we developed a novel cell therapy using bone marrow cells; autologous bone marrow cell infusion (ABMi) therapy. We previously described the possible action mechanism of ABMi therapy in the cirrhotic liver, and showed the timeline and results of clinical studies of ABMi therapy. We have also carried out other clinical studies using bone marrow cells and granulocyte colony-stimulating factor. Here, we report a new randomized clinical trial to evaluate the effects of ABMi therapy. However, ABMi therapy may not be possible in patients who are unable to undergo general anesthesia; therefore, we have started to develop a next-generation stem cell therapy using cultured mesenchymal stem cells. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
43. Molecular targeting of CSN5 in human hepatocellular carcinoma: a mechanism of therapeutic response.
- Author
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Lee, Y-H, Judge, A D, Seo, D, Kitade, M, Gómez-Quiroz, L E, Ishikawa, T, Andersen, J B, Kim, B-K, Marquardt, J U, Raggi, C, Avital, I, Conner, E A, MacLachlan, I, Factor, V M, and Thorgeirsson, S S
- Subjects
LIVER cancer ,GENE expression ,SMALL interfering RNA ,APOPTOSIS ,CELL cycle ,PHENOTYPES ,CYCLIN-dependent kinases ,INTEGRINS ,PHOSPHORYLATION ,WESTERN immunoblotting ,TRANSFORMING growth factors - Abstract
Development of targeted therapy for hepatocellular carcinoma (HCC) remains a major challenge. We have recently identified an elevated expression of the fifth subunit of COP9 signalosome (CSN5) in early HCC as compared with dysplastic stage. In the present study, we explored the possibility of CSN5 being a potential therapeutic target for HCC. Our results show that CSN5 knockdown by small-interfering (si) RNA caused a strong induction of apoptosis and inhibition of cell-cycle progression in HCC cells in vitro. The down-regulation of CSN5 was sufficient to interfere with CSN function as evidenced by the accumulation of neddylated Cullin 1 and changes in the protein levels of CSN-controlled substrates SKP2, p53, p27 and nuclear factor-κB, albeit to a different degree depending on the HCC cell line, which could account for the CSN5 knockdown phenotype. The transcriptomic analysis of CSN5 knockdown signature showed that the anti-proliferative effect was driven by a common subset of molecular alterations including down-regulation of cyclin-dependent kinase 6 (CDK6) and integrin β1 (ITGB1), which were functionally interconnected with key oncogenic regulators MYC and TGFβ1 involved in the control of proliferation, apoptotic cell death and HCC progression. Consistent with microarray analysis, western blotting revealed that CSN5 depletion increased phosphorylation of Smad 2/3, key mediators of TGFβ1 signaling, decreased the protein levels of ITGB1, CDK6 and cyclin D1 and caused reduced expression of anti-apoptotic Bcl-2, while elevating the levels of pro-apoptotic Bak. A chemically modified variant of CSN5 siRNA was then selected for in vivo application based on the growth inhibitory effect and minimal induction of unwanted immune response. Systemic delivery of the CSN5 3/8 variant by stable-nucleic-acid-lipid particles significantly suppressed the tumor growth in Huh7-luc
+ orthotopic xenograft model. Taken together, these results indicate that CSN5 has a pivotal role in HCC pathogenesis and maybe an attractive molecular target for systemic HCC therapy. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
44. Quantification of myocardial perfusion SPECT using freeware package (cardioBull)
- Author
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Okuda K, Nakajima K, Hosoya T, Ishikawa T, Matsuo S, Kawano M, Taki J, and Kinuya S
- Published
- 2011
45. Bax inhibitor-1: a highly conserved endoplasmic reticulum-resident cell death suppressor.
- Author
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Ishikawa, T., Watanabe, N., Nagano, M., Kawai-Yamada, M., and Lam, E.
- Subjects
- *
CELL death , *PLANTS , *PLANT genomes , *ENDOPLASMIC reticulum , *CYTOCHROME c , *AMINO acid metabolism - Abstract
In spite of fundamental differences between plant and animal cells, it is remarkable that some cell death regulators that were identified to control cell death in metazoans can also function in plants. The fact that most of these proteins do not have structural homologs in plant genomes suggests that they may be targeting a highly conserved 'core' mechanism with conserved functions that is present in all eukaryotes. The ubiquitous Bax inhibitor-1 (BI-1) is a common cell death suppressor in eukaryotes that has provided a potential portal to this cell death core. In this review, we will update the current status of our understanding on the function and activities of this intriguing protein. Genetic, molecular and biochemical studies have so far suggested a consistent view that BI-1 is an endoplasmic reticulum (ER)-resident transmembrane protein that can interact with multiple partners to alter intracellular Ca2+ flux control and lipid dynamics. Functionally, the level of BI-1 protein has been hypothesized to have the role of a rheostat to regulate the threshold of ER-stress inducible cell death. Further, delineation of the cell death suppression mechanism by BI-1 should shed light on an ancient cell death core-control pathway in eukaryotes, as well as novel ways to improve stress tolerance. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
46. Metallic-like droplets produced by irradiating rare-gas clusters with free electron laser pulses.
- Author
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Yao, M., Nagaya, K., Iwayama, H., Fukuzawa, H., Ueda, K., Nagasono, M., and Ishikawa, T.
- Published
- 2011
- Full Text
- View/download PDF
47. Radiological characterization of commercially available 'radon spa sources'.
- Author
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Ishikawa, T., Hosoda, M., Sorimachi, A., Tokonami, S., Katoh, S., and Ogashiwa, S.
- Subjects
- *
RADIOCHEMISTRY , *RADON , *BATHS , *GAMMA rays , *RADIATION dosimetry , *AIR flow - Abstract
Nowadays, artificial 'radon spa sources' for home baths are commercially available. Although these sources could give a potential radiation exposure to the users, few studies have been reported on their radiological measurements. In the present study, five types of radon spa sources were collected and their radiological characterization was investigated. The followings were estimated for these samples: (1) radon emanation coefficients (dry and water-saturated conditions), (2) surface γ-ray dose rate, (3) surface count rates for α- and β-rays, (4) activity concentrations of Ra, Th and K, and (5) concentrations of radon and thoron generated from the sources located in an air flow system. The activity concentrations were very high (except for one sample (named 'sample B'), although radon emanation coefficient was low compared with soil. This leads to high concentrations of radon/thoron generated from the sample. The maximum surface γ-ray dose rate was observed for sample A (2.7 μGy h). If people stay very close to the sample for a long time, the exposure might be significant. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
48. Pretransplant serum ferritin and C-reactive protein as predictive factors for early bacterial infection after allogeneic hematopoietic cell transplantation.
- Author
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Kanda, J., Mizumoto, C., Ichinohe, T., Kawabata, H., Saito, T., Yamashita, K., Kondo, T., Takakura, S., Ichiyama, S., Uchiyama, T., and Ishikawa, T.
- Subjects
FERRITIN ,C-reactive protein ,BACTERIAL diseases ,HEMATOPOIETIC stem cell transplantation ,STEM cell transplantation ,ANTIBACTERIAL agents - Abstract
Although fluoroquinolones or other antibiotics are commonly used to prevent bacterial infections after hematopoietic cell transplantation (HCT), because of the growing presence of multidrug-resistant microorganisms, it is important to identify patients who are more likely to benefit from antibacterial prophylaxis. To evaluate risk factors for early bacterial infection after allogeneic HCT, we retrospectively analyzed clinical data for 112 consecutive adult patients with hematological malignancies who received transplants without any antibacterial prophylaxis. The cumulative incidence of bacterial infection at 30 days after transplantation was 16%. Among various pre-transplant factors, only high serum ferritin (>700 ng/mL, 47 patients) and high C-reactive protein (CRP) (>0.3 mg/dL, 28 patients) levels were significantly associated with the development of bacterial infection in a multivariate analysis (hazard ratio (95% confidence interval): ferritin, 4.00 (1.32-12.17); CRP, 3.64 (1.44-9.20)). In addition, septic shock and sepsis with organ failure were exclusively observed in patients who had high ferritin and/or high CRP levels. These results suggest that pretransplant serum ferritin and CRP levels can be useful markers for predicting the risk of early bacterial infection after allogeneic HCT. It may be prudent to limit antibacterial prophylaxis to patients with predefined risk factors to ensure the safety of HCT with the use of fewer antibiotics. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
49. c-MYC overexpression with loss of Ink4a/Arf transforms bone marrow stromal cells into osteosarcoma accompanied by loss of adipogenesis.
- Author
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Shimizu, T, Ishikawa, T, Sugihara, E, Kuninaka, S, Miyamoto, T, Mabuchi, Y, Matsuzaki, Y, Tsunoda, T, Miya, F, Morioka, H, Nakayama, R, Kobayashi, E, Toyama, Y, Kawai, A, Ichikawa, H, Hasegawa, T, Okada, S, Ito, T, Ikeda, Y, and Suda, T
- Subjects
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TRANSCRIPTION factors , *MESENCHYMAL stem cells , *CANCER invasiveness , *OSTEOSARCOMA , *LABORATORY mice , *GENE expression , *GENETIC engineering - Abstract
The development of cancer is due to the growth and proliferation of transformed normal cells. Recent evidence suggests that the nature of oncogenic stress and the state of the cell of origin critically affect both tumorigenic activity and tumor histological type. However, this mechanistic relationship in mesenchymal tumors is currently largely unexplored. To clarify these issues, we established a mouse osteosarcoma (OS) model through overexpression of c-MYC in bone marrow stromal cells (BMSCs) derived from Ink4a/Arf (−/−) mice. Single-cell cloning revealed that c-MYC-expressing BMSCs are composed of two distinctly different clones: highly tumorigenic cells, similar to bipotent-committed osteochondral progenitor cells, and low-tumorigenic tripotent cells, similar to mesenchymal stem cells (MSCs). It is noteworthy that both bipotent and tripotent cells were capable of generating histologically similar, lethal OS, suggesting that both committed progenitor cells and MSCs can become OS cells of origin. Shifting mesenchymal differentiation by depleting PPARγ in tripotent MSC-like cells and overexpressing PPARγ in bipotent cells affected cell proliferation and tumorigenic activity. Our findings indicate that differentiation potential has a key role in OS tumorigenic activity, and that the suppression of adipogenic ability is a critical factor for the development of OS. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
50. Significance of E-cadherin expression in triple-negative breast cancer.
- Author
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Kashiwagi, S., Yashiro, M., Takashima, T., Nomura, S., Noda, S., Kawajiri, H., Ishikawa, T., Wakasa, K., and Hirakawa, K.
- Subjects
BREAST cancer prognosis ,CADHERINS ,PROGESTERONE receptors ,HER2 protein ,IMMUNOHISTOCHEMISTRY ,LOGISTIC regression analysis ,SURGICAL excision ,PROTEIN analysis ,CELL receptors ,GLYCOPROTEINS ,BREAST tumors - Abstract
Purpose: Triple-negative breast cancer (TNBC), a subtype of breast cancer that is oestrogen receptor (ER) negative, progesterone receptor (PR) negative, and human epidermal growth factor receptor 2 (HER2) negative, has a poor prognosis. Although a correlation between E-cadherin expression level and outcome has been demonstrated among all types of breast cancer, little is known about the significance of E-cadherin expression levels in TNBC.Methods: A total of 574 patients who had undergone a resection of a primary breast cancer except for invasive lobular carcinomas were enrolled in this study. Expressions of ER, PR, HER2, and E-cadherin were assessed by immunohistochemistry. We examined the association between TNBC and other clinicopathological variables and evaluated the significance of the E-cadherin expression.Results: Among the 574 breast cancer cases, 123 (21.4%) revealed a triple-negative phenotype. Patients with TNBC experienced more frequent lymph node metastasis (P=0.024) and a poorer prognosis (P<0.001) in comparison with non-TNBC patients. Triple-negative breast cancer was an independent prognostic factor. Reduced levels of E-cadherin were observed in 238 (41.5%) of the 574 breast cancer cases. E-cadherin reduction was significantly frequent in cases of TNBC (P<0.001) and lymph node metastasis (P=0.032). Furthermore, in the 123 TNBC cases, the prognosis of patients with an E-cadherin-negative expression was significantly worse than that of E-cadherin-positive patients (P=0.0265), especially for those in clinical stage II (P=0.002). A multivariate logistic regression analysis showed a reduction of the E-cadherin expression to be an independent prognostic factor (P=0.046).Conclusion: E-cadherin expression may be a useful prognostic marker for classifying subgroups of TNBC. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
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