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In this study, a regional irrigation schedule optimization method was proposed and applied in Fengqiu County in the North China Plain, which often suffers serious soil water drainage and nitrogen (N) leaching problems caused by excessive irrigation. The irrigation scheduling method was established by integrating the 'checkbook irrigation method' into a GIS-coupled soil water and nitrogen management model (WNMM) as an extension. The soil water and crop information required by the checkbook method, and previously collected from field observations, was estimated by the WNMM. By replacing manually observed data with simulated data from WNMM, the application range of the checkbook method could be extended from field scale to regional scale. The WNMM and the checkbook irrigation method were both validated by field experiments in the study region. The irrigation experiment in fluvo-aquic soil showed that the checkbook method had excellent performance; soil water drainage and N leaching were reduced by 83.1 and 85.6%, respectively, when compared with local farmers' flood irrigation. Using the validated WNMM, the performance of checkbook irrigation in an entire winter wheat and summer maize rotation was also validated: the average soil water drainage and N leaching in four types of soils decreased from 331 to 75 mm year and 47.7 to 9.3 kg hayear, respectively; and average irrigation water use efficiency increased from 26.5 to 57.2 kg ha mm. The regional irrigation schedule optimization method based on WNMM was applied in Fengqiu County. The results showed a good effect on saving irrigation water, decreasing soil water drainage and then saving agricultural inputs. In a typical meteorological year, it could save >110 mm of irrigation water on average, translating to >7.26 × 10 m of agricultural water saved each year within the county. Annual soil water drainage was reduced to <143 mm and N leaching to <27 kg ha in most soils, all of which were significantly lower than local farmers' flood irrigation. In the mean time, crop yield also had an average increase of 2,890 kg ha when checkbook irrigation was applied. [ABSTRACT FROM AUTHOR]

Purpose: Organic and inorganic fertilizers are used primarily to increase nutrient availability to plants. Monitoring balanced versus unbalanced fertilization effects on soil microbes could improve our understanding of soil biochemical processes and thus help us to develop sound management strategies. The objective of this study was to investigate the effects of long-term fertilization regimes on soil microbial community functional diversity, metabolic activity, and metabolic quotient and to find out the main factors that influence these parameters. Materials and methods: A long-term fertilization experiment established in a sandy loam soil at northern China has received continuous fertilization treatments for more than 20 years, including control, mineral fertilizers of NK, PK, NP, and NPK, organic amendment (OA), and half organic amendment plus half mineral fertilizer (1/2 OM). Top soil samples (0-15 cm) from four individual plots per treatment were collected for the analysis of chemical properties and microbial parameters. Microbial biomass C was analyzed using the fumigation-extraction method. Invertase activity and basal respiration were determined based on incubation method. Then, the microbial metabolic quotient was calculated as the ratio of basal respiration to microbial biomass C. To this end, microbial functional diversity was evaluated using the community level physiological profile method by Biolog Eco-microplate. Results and discussion: Higher microbial biomass C, invertase activity, and basal respiration, but lower microbial metabolic quotient, were observed in P-fertilized soils, and OA had significantly greater ( P < 0.05) impacts on the biomass, activity, and quotient compared with mineral fertilizers. Both the sole-carbon-source utilization activity and the functional diversity of soil microbial community were significantly increased ( P < 0.05) by balanced fertilization (NPK, OA, or 1/2 OM), and species richness of community and relative abundance of the most common species in the K-deficient (NP) treatment were also significantly increased ( P < 0.05). Principal component analysis and redundancy analysis showed that both organic and mineral fertilizers could affect microbial parameters by increasing soil organic C contents, and P was the key factor to increase soil microbial diversity and soil fertility. Conclusions: Long-term balanced fertilization greatly increased soil microbial biomass, functional diversity, and invertase activity and played an important role in decreasing soil microbial metabolic quotient, while P could be considered as the key factor to control soil microbial diversity as well as soil fertility. With regard to the different effects of OA and mineral fertilizer on soil organic C contents and root exudates, combined application of mineral and organic fertilizers is recommended in the region. [ABSTRACT FROM AUTHOR]

The P efficiency, crop yield, and response of wheat to arbuscular mycorrhizal fungus (AMF) Glomus caledonium were tested in an experimental field with long-term (19 years) fertilizer management. The experiment included five fertilizer treatments: organic amendment (OA), half organic amendment plus half mineral fertilizer (1/2 OM), mineral fertilizer NPK, mineral fertilizer NK, and the control (without fertilization). AMF inoculation responsiveness (MIR) of wheat plants at acquiring P were estimated by comparing plants grown in unsterilized soil inoculated with G. caledonium and in untreated soil containing indigenous AMF. Without AMF inoculation, higher crop yields but lower colonization rates were observed in the NPK and two OA-inputted treatments, and NPK had significantly ( P < 0.05) lower impacts on organic C and available P in soils and thereby P acquisition of wheat plants compared with OA and 1/2 OM. G. caledonium inoculation significantly ( P < 0.05) increased colonization rates with the NPK and two P-deficient treatments but significantly ( P < 0.05) increased vegetative biomass, crop yield, and P acquisition of wheat as well as soil alkaline phosphatase (ALP) activity, only with the NPK treatment. This gave an MIR of ca. 45% on total P acquisition of wheat plants. There were no other remarkable MIRs. It suggested that the MIR is determined by soil available P status, and rational combination of AMF with chemical NPK fertilizer can compensate for organic amendments by improving P-acquisition efficiency in arable soils. [ABSTRACT FROM AUTHOR]

Abstract  Insulin-like growth factor-I (IGF-I) plays a fundamental role in postnatal mammalian growth, development, and metabolism. The mammalian IGF-I gene contains at least six exons from which several different mRNAs are transcribed. In this study, IGF-I mRNA in Songliao black pig liver was investigated using RLM-RACE. Using a 3′-RACE technique, we determined that all mRNA transcripts lacked exon 5 sequence and contained only exon 6 sequence. Using a 5′-RACE technique, we investigated the presence of class 1 and class 2 IGF-I mRNAs. In several other species, the class 1 and class 2 IGF-I polypeptides are generated from mRNAs containing exon 1 or exon 2, respectively. Both class 1 and class 2 IGF-I mRNAs were identified in Songliao black pig liver. Transcription is initiatated upstream of exons 1 and 2 at multiple dispersed start sites to yield two distinct IGF-I mRNA transcript classes which differ in the precursor peptides predicted from their individual leader sequences. Tissue distribution of Songliao black pig class 1 and class 2 IGF-I mRNA was investigated by real-time RT-PCR. Both classes of IGF-I mRNA were expressed in a variety of tissues, however, class 1 IGF-I mRNA was more abundant than class 2 in all tissues. [ABSTRACT FROM AUTHOR]

The article focuses on the concentration and chemical fractions of copper, zinc, nickel and chromium in Japanese andisols, which had accumulated swine composts and chemical fertilizers for six years. The unique properties of andisols including high anion exchange capacity, low bulk density, and high organic matter content may influence the accruement of heavy metals. The potential risks of the aggregation of heavy metals as well as its impact on plant tissues, soils and surface waters are also discussed.

A pot experiment was conducted in a glasshouse to clarify and quantify the effect of plant part, water regime, growth period, and cultivar on carbon isotope discrimination (CID), and to analyze the relationship between CID, stomatal behavior and water-use efficiency (WUE). The experiment was comprised of two upland rice (Oryza sativa L.) cultivars and three water regimes (100, 70, and 40% of saturation moisture) in a completely randomized design. Plants were harvested at tillering, flowering, and maturity. No significant cultivar differences in above-ground dry matter-based WUE (WUEA) and total dry matter-based WUE (WUET) were observed. WUEA (and WUET) increased with water stress up to tillering, but decreased with water stress after tillering. Significant cultivar differences in CID in all the analyzed plant parts were observed at all harvest times. Reduction in CID with water stress was greatest at tillering, and the effect was less pronounced at flowering and at maturity. At each harvest, the effect was most pronounced in newly developed plant parts. Root and grain tended to have the lowest CID values, and stem the highest, at all harvest times. A negative relationship was observed between CID measured at tillering and WUEA (and WUET) measured over the period from seedling to tillering, whereas a reverse relationship was obtained between CID measured at flowering and WUEA (and WUET) measured over the period from tillering to flowering, and an unclear relationship between CID measured at maturity and WUEA (and WUET) measured over the period from flowering to maturity. The ratio of the intercellular and atmospheric concentration of CO2 (Ci/Ca) were closely associated with CID throughout the water regimes when one cultivar was considered, however, cultivar differences in CID were not related to variations in Ci/Ca. The results indicate that significant cultivar difference existed in CID in all the analyzed plant parts at all harvest times, while corresponding difference in WUEA (and WUET) between the cultivars was not necessarily consistent. Abbreviations: WUE – water-use efficiency; WUEi – instantaneous WUE (or leaf transpiration efficiency); ADM – above-ground dry matter; TDM – total dry matter; WUEA – ADM-based WUE; WUET – TDM-based WUE}; CID – carbon isotope discrimination; NL – the newest leaves; FEL – recently fully expanded leaves; FL – flag leaves; P – photosynthesis rate; g – leaf stomatal conductance to water vapor; Ci – intercellular CO2 concentration; Ca – atmospheric CO2 concentration; T – transpiration rate; gs – total conductance of CO2 [ABSTRACT FROM AUTHOR]