1. Regulation of compound leaf development in mungbean (Vigna radiata L.) by CUP-SHAPED COTYLEDON/NO APICAL MERISTEM (CUC/NAM) gene.
- Author
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Jiao, Keyuan, Li, Xin, Guo, Yafang, Guan, Yining, Guo, Wuxiu, Luo, Da, Hu, Zhubing, and Shen, Zhenguo
- Subjects
MUNG bean ,BEANS ,GENE expression ,MOLECULAR genetics ,PLANT growth - Abstract
Main conclusion: The results provide a significant verification of functional redundancy and diversity of CUC/NAM genes in legumes.The CUP-SHAPED COTYLEDON/NO APICAL MERISTEM (CUC/NAM) orthologs play key roles for plant organ boundary formation and organ development. Here, we performed a forward screen of the gamma irradiation mutagenesis population in mungbean and characterised a mutant, reduced rachis and fused leaflets (rrf1), which gave rise to the formation of compound leaves with reduced rachis and fused leaflets. Map-based cloning revealed that RRF1 encoded a CUC/NAM protein in mungbean. Phylogenetic analysis indicated that legume CUC1/CUC2 genes were classified as belonging to two subclades, and there are different copies of CUC1/CUC2 genes in legumes. Transcriptomic analysis showed that expression levels of a set of developmental regulators, including class I KNOTTED-LIKE HOMEOBOXI (KNOXI) gene and LATERAL ORGAN BOUNDARIES DOMAIN (LBD) gene, were altered in rrf1 mutants compared to the wild-type plants. Furthermore, rrf1 genetically interacted with heptafoliate leaflets1 (hel1), a mutant displaying a seven-leaflet compound leaf, to regulate leaf development in mungbean. Our results suggest functional redundancy and diversity of two subclades of CUC1/CUC2 genes in legumes, following the duplication of an ancestral gene. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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