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Purpose: Facing the high-power requirements of large-scale or super-scale scale vibratory machines, the multi-machine parallel technology by adding the number of exciters is a feasible solution. In the paper, the self-synchronous characteristics and Sommerfeld effect of a vibration system driven by four non-ideal induction motors rotating in arbitrary directions on a rigid body are investigated to expand the application of the multi-machine parallel technology in large vibratory machines. Methods: The dynamic coupling model of the whole vibration system is established by using the Lagrange method. A modified direct separation of motion method is innovatively applied to transfer the complex synchronous motion problem of four time-varying coupling dynamic systems to that of four time-invariant systems. The synchronization and stability conditions of the modified system are given by using the perturbation method. Results: The Sommerfeld effect and self-synchronization near the natural frequency of the vibration system interact with each other. The synchronous states of four motors will change before and after the natural frequency of the vibration system. The rotating directions of four motors influence the synchronous states of four motors largely. Conclusion: To implement the purpose of enlarge the excitation force by increasing the number of exciters, four motors should work before the system' natural frequency. For vibration attenuation, four motors should work after the system natural frequency and the power of the motors should be very large to avoid the capture of motor speed. Self-synchronization of four motors is helpful for vibration attenuation. [ABSTRACT FROM AUTHOR]

Introduction: A pan-genotypic and effective treatment regimen for patients with chronic hepatitis C virus (HCV) infection remains an unmet medical need in China. Alfosbuvir is a novel potent HCV NS5B polymerase inhibitor in development for the treatment of chronic HCV infection. We conducted a phase 3 study to evaluate the efficacy and safety of alfosbuvir in combination with daclatasvir in Chinese patients with HCV infection. Methods: All patients received 600 mg alfosbuvir tablets plus 60 mg daclatasvir tablets once daily for 12 weeks. The primary endpoint was sustained virological response 12 weeks after the end of treatment (SVR12). A follow-up visit was done at week 4 and 12, and those who achieved SVR12 were followed up at post-treatment week 24. Results: Of the 326 patients who received at least one dose of the study drug, 320 (98.2% [95% confidence interval (CI): 96.5%–99.5%]) achieved sustained virological response at post-treatment week 12 (SVR12), which was superior to the historical SVR12 rate of 88% (p < 0.0001). The SVR12 rates were similar regardless of most baseline characteristics. The most common adverse event (AE) (≥ 10%) was hypercholesterolemia. Serious adverse events (SAEs) were reported in 25 (7.7%) patients, none of which was judged to be related to the study drug. The majority of AEs were mild to moderate in severity. Conclusions: Alfosbuvir plus daclatasvir for 12 weeks was highly effective and safe in Chinese patients infected with HCV genotype 1, 2, 3, or 6, suggesting that this regimen could be a promising option for HCV treatment in China irrespective of genotype. Trial Registration: ClinicalTrial.gov identifier, NCT04070235. [ABSTRACT FROM AUTHOR]

An ultimate goal of electron paramagnetic resonance (EPR) spectroscopy is to analyze molecular dynamics in place where it occurs, such as in a living cell. The nanodiamond (ND) hosting nitrogen-vacancy (NV) centers will be a promising EPR sensor to achieve this goal. However, ND-based EPR spectroscopy remains elusive, due to the challenge of controlling NV centers without well-defined orientations inside a flexible ND. Here, we show a generalized zero-field EPR technique with spectra robust to the sensor's orientation. The key is applying an amplitude modulation on the control field, which generates a series of equidistant Floquet states with energy splitting being the orientation-independent modulation frequency. We acquire the zero-field EPR spectrum of vanadyl ions in aqueous glycerol solution with embedded single NDs, paving the way towards in vivo EPR. Nanodiamonds containing NV centers are promising electron paramagnetic resonance sensors, however applications are hindered by their random orientation. Qin et al. propose a new protocol that makes the technique insensitive to the sensor's orientation and present a proof-of-principle in situ demonstration. [ABSTRACT FROM AUTHOR]

Let U ħ (g ^) be the untwisted affinization of a symmetrizable quantum Kac-Moody algebra U ħ (g) . For ℓ ∈ C , we construct an ħ -adic quantum vertex algebra V g ^ , ħ (ℓ , 0) , and establish a one-to-one correspondence between ϕ -coordinated V g ^ , ħ (ℓ , 0) -modules and restricted U ħ (g ^) -modules of level ℓ . Suppose that ℓ is a positive integer. We construct a quotient ħ -adic quantum vertex algebra L g ^ , ħ (ℓ , 0) of V g ^ , ħ (ℓ , 0) , and establish a one-to-one correspondence between certain ϕ -coordinated L g ^ , ħ (ℓ , 0) -modules and restricted integrable U ħ (g ^) -modules of level ℓ . Suppose further that g is of finite type. We prove that L g ^ , ħ (ℓ , 0) / ħ L g ^ , ħ (ℓ , 0) is isomorphic to the simple affine vertex algebra L g ^ (ℓ , 0) . [ABSTRACT FROM AUTHOR]

Batch normalization is one of the most widely used components in deep neural networks. It can accelerate training, and boost model performance on normal samples. However, batch normalization induces vulnerability to models on adversarial examples, especially in medical images, and the reason is still not clear. In this paper, we aim to explain the vulnerability aroused by batch normalization under adversarial images. Specifically, we first discover that both natural and medical images contain a large number of trivial features, whose weights will be enlarged under adversarial attacks, and batch normalization can further enlarge their weights. Additionally, we find that batch normalization will reduce the inter-class margin of high-level features, leading to less tolerance to adversarial perturbations, thereby decreasing the model robustness. Moreover, we hypothesize that the smaller inter-class margin, the more difficult to attain the optimal classification space, which means batch normalization will restrict the performance of adversarial training. This further verifies that a narrower inter-class margin induced by batch normalization reduces the model robustness. Experiments on four benchmark datasets demonstrate our discovery, interpretation and hypothesis. [ABSTRACT FROM AUTHOR]

Glioma treatment remains a challenge owing to unsatisfactory targeted chemotherapy, where the blood–brain barrier (BBB) hinders the efficient uptake of therapeutics into the brain. Vasculogenic mimicry (VM) formed by invasive glioma cells negatively affects the treatment of glioma. Herein, we developed a targeted biomimetic drug delivery system comprising a doxorubicin-loaded platelet membrane-coated nanogel (DOX@PNGs). The nanogels provide great redox/pH dual responsiveness, while the platelet membrane (PLTM) promotes stability and circulation time. In vitro cellular uptake and in vivo imaging experiments demonstrated that the DOX@PNGs delivery system could penetrate the BBB, target gliomas, and destruct VM. DOX@PNGs increased drug penetration and prolonged mouse survival time during the treatment of orthotopic gliomas. These results indicate this biomimetic drug delivery system to be promising for glioma treatment and may be clinically translated in the future. [ABSTRACT FROM AUTHOR]

As pioneering Fe3O4 nanozymes, their explicit peroxidase (POD)-like catalytic mechanism remains elusive. Although many studies have proposed surface Fe2+-induced Fenton-like reactions accounting for their POD-like activity, few have focused on the internal atomic changes and their contribution to the catalytic reaction. Here we report that Fe2+ within Fe3O4 can transfer electrons to the surface via the Fe2+-O-Fe3+ chain, regenerating the surface Fe2+ and enabling a sustained POD-like catalytic reaction. This process usually occurs with the outward migration of excess oxidized Fe3+ from the lattice, which is a rate-limiting step. After prolonged catalysis, Fe3O4 nanozymes suffer the phase transformation to γ-Fe2O3 with depletable POD-like activity. This self-depleting characteristic of nanozymes with internal atoms involved in electron transfer and ion migration is well validated on lithium iron phosphate nanoparticles. We reveal a neglected issue concerning the necessity of considering both surface and internal atoms when designing, modulating, and applying nanozymes. The mechanism of peroxidase-like Fe3O4 nanozymes remains elusive. Here, the authors show the electron transfer mechanism of Fe(II) ions to regenerate surface Fe(II) and the related phase transformation and depletion of activity. [ABSTRACT FROM AUTHOR]

Objective: Adenoid or adenotonsillar hypertrophy (AATH) causes upper airway obstruction, leading to cardiovascular complications. This meta-analysis was conducted to evaluate the efficacy of adenoidectomy or adenotonsillectomy (AATE) on the cardiovascular system. Methods: Using the Cochrane Central Register of Controlled Trials, PubMed, and EMBASE databases, we identified studies involving a comparison of preoperative and postoperative cardiovascular function in children with AATH. The Cochrane Collaboration's Review Manager 5.3 software was used for meta-analysis. Results: A total of 13 studies with 706 participants were included. The meta-analysis demonstrated a significant reduction in mean pulmonary artery pressure (mPAP) of patients after AATE compared with preoperative values. The left ventricular myocardial function index (LVMPI) and the right ventricular myocardial function index (RVMPI) showed a significant decrease after the operation. Moreover, AATE prominently increased left ventricular ejection time (LVET) and right ventricular ejection time (RVET) and reduced the left ventricular interventricular septum diameter (LVIVSD) and the right ventricular end-diastolic diameter (RVedD). There was no significant difference in mPAP, LVMPI, RVMPI, LVET, RVET, LVIVSD, and RVedD between postoperative patients and healthy children (P > 0.05). Conclusion: AATE can improve cardiovascular function in pediatric patients with AATH. Specifically, it reduces mPAP and LVMPI/RVMPI in pediatric patients. Furthermore, AATE increases LVET and RVET and reduces LVIVSD and RVedD. [ABSTRACT FROM AUTHOR]

Limited treatment options are available for relapsed or refractory diffuse large B cell lymphoma (RR DLBCL). Few clinical studies have reported the use of Ibrutinib, a covalent Bruton Tyrosine kinase (BTK) inhibitor, in RR DLBCL. There are relatively few clinical studies about Ibrutinib in RR DLBCL now. We retrospectively investigated the safety and efficacy of Ibrutinib (alone or in combination with other drugs) in patients with RR DLBCL. We reviewed the medical records of 40 RR DLBCL patients who received Ibrutinib alone or in combination with other drugs in our hospital from June 2018 to August 2020. The objective response rate (ORR) of RR DLBCL patients on Ibrutinib was 22.5%. The median progression free survival time (PFS) was 13.0 months (95% CI 8.914–17.086), and the median overall survival time (OS) was 15.0 months (95% CI 11.931–18.089). Rash (25.0%) and fatigue (25.0%) were the most common adverse reactions in this study. The application of Ibrutinib to patients with RR DLBCL has good short-term efficacy, and the adverse reactions are well tolerated. Combined treatment of Ibrutinib with other drugs has been found to more effective than Ibrutinib therapy alone. [ABSTRACT FROM AUTHOR]

Peroxisome proliferator activated receptors (PPAR) are kinds of key transcriptional factors in regulating LC-PUFA biosynthesis. Until now, little is known about PPAR in marine molluscs as well as in other invertebrates. Sinonovacula constricta is the first marine mollusc that is proved to possess the complete LC-PUFA biosynthetic pathway, and it can be a perfect representative to clarify this situation. In this study, the molecular properties of S. constricta PPAR were characterized, and corresponding potential regulatory roles in S. constricta Δ6 fatty acyl desaturase (Fad) transcription were estimated by dual luciferase assay. Results showed that two PPAR homologs (PPAR_a and _b) were identified in S. constricta. They both contain typical features of vertebrate PPAR, suggesting highly conserved functional regions in PPAR. By phylogenetic comparison, they are clearly different with vertebrate PPAR and can be divided into two distinctive sub-groups. Moreover, they show a high expression level in gill, labial palps, mantle and intestine. Their down-regulated expressions in trochophore larva and veliger larva might be attributed to food-deprivation. Additionally, the transcriptional activity of S. constricta Δ6 Fad promoter was significantly activated by both PPAR_a and _b, indicating that S. constricta PPAR might play a role in the regulation of LC-PUFA biosynthesis. To our knowledge, this is the first systematic analysis of PPAR in a marine mollusc. The results will provide a valuable reference for further researches on the function of marine molluscan PPAR and their underlying mechanisms in regulating LC-PUFA biosynthesis. [ABSTRACT FROM AUTHOR]

Let g be a (twisted or untwisted) affine Kac-Moody algebra, and μ be a diagram automorphism of g. In this paper, we give an explicit realization for the universal central extension ĝ[μ] of the twisted loop algebra of g with respect to μ, which provides a Moody-Rao-Yokonuma presentation for the algebra ĝ[μ] when μ, is non-transitive, and the presentation is indeed related to the quantization of twisted toroidal Lie algebras. [ABSTRACT FROM AUTHOR]

High fidelity single-shot readout of qubits is a crucial component for fault-tolerant quantum computing and scalable quantum networks. In recent years, the nitrogen-vacancy (NV) center in diamond has risen as a leading platform for the above applications. The current single-shot readout of the NV electron spin relies on resonance fluorescence method at cryogenic temperature. However, the spin-flip process interrupts the optical cycling transition, therefore, limits the readout fidelity. Here, we introduce a spin-to-charge conversion method assisted by near-infrared (NIR) light to suppress the spin-flip error. This method leverages high spin-selectivity of cryogenic resonance excitation and flexibility of photoionization. We achieve an overall fidelity > 95% for the single-shot readout of an NV center electron spin in the presence of high strain and fast spin-flip process. With further improvements, this technique has the potential to achieve spin readout fidelity exceeding the fault-tolerant threshold, and may also find applications on integrated optoelectronic devices. The NV centre in diamond has been used extensively in quantum information processing; however fault-tolerant readout of its spin remains challenging. Here, Zhang et al demonstrate a robust scheme that achieves high-fidelity readout via spin to charge conversion. [ABSTRACT FROM AUTHOR]

Summary: Purpose Apatinib, a new tyrosine kinase inhibitor targeting vascular endothelial growth factor receptor-2, has shown promising efficacy against several solid cancers, but evidence of its efficacy against relapsed and refractory nasopharyngeal carcinoma is limited. We investigated the efficacy and safety of apatinib for relapsed and refractory nasopharyngeal carcinoma in an open-label, single-arm, phase II clinical trial. Fifty-one patients with relapsed and refractory nasopharyngeal carcinoma in the First Affiliated Hospital, Zhengzhou University, who met the inclusion criteria were enrolled in the study. All patients received apatinib at an initial dose of 500 mg daily (1 cycle = 28 days). The primary and secondary endpoints were overall response rate, progression-free survival, and overall survival. We evaluated treatment effects and recorded apatinib-related adverse events by performing regular follow-ups and workup. The overall response rate (complete and partial responses) was 31.37% (16/51). The median overall survival and progression-free survival were 16 (95% CI, 9.32–22.68) and 9 months (95% CI, 5.24–12.76), respectively. Most patients tolerated treatment-related adverse events of grades 1 and 2; hypertension (29, 56.86%), proteinuria (25, 49.02%), and hand–foot syndrome (27, 52.94%) were the most common adverse events. There were no treatment-related deaths. Apatinib showed good efficacy and safety in patients with relapsed and refractory NPC. [ABSTRACT FROM AUTHOR]

This study aims to produce a biodegradable active packaging film that is pH sensitive, and has a good antioxidant and antimicrobial activity. To do this, a novel phenolic extract was interfacially assembled onto a cellulose film, resulting in a film with a pH-triggered release mechanism of the active polyphenol agent. First, an aqueous extraction of cashew nut testa (CTE) was performed and subsequently, the CTE was characterized. The disc diffusion assay showed that CTE exhibited antimicrobial activity towards the food pathogens Escherichia coli (6 mm) and Staphylcoccus aureus (12 mm). CTE was also cytotoxic against cancer HepG2 and HEK293 cells, reducing the viability to 52% and 47%, respectively. It was incorporated into a cellulose-based packaging film, prepared from the by-product, sugarcane bagasse (SC) through interfacial assembly. The incorporation of CTE resulted in a film with good antimicrobial activity, excellent antioxidant content (91%), and has extremely high thermal stability (290 °C). FTIR indicated the formation of hydrogen bond between the SC cellulose-based film and CTE. The hydrogen bonds formed between the cellulose film and CTE became the driving force behind the pH-triggered release mechanism. It was found that the active agent, tannic acid, could be controllably released from the film, depending on the pH of the environment. Our strategy to produce a cellulose-based film impregnated with a phenolic extract, using interfacial assembly, resulted in an active packaging film with pH-triggered release mechanism. This film could be useful to extend the shelf life of perishable food items. [ABSTRACT FROM AUTHOR]

Graphene, the 2-D allotrope of carbon, is well known for its remarkable electronic and optical properties. Nitrogen doping can manipulate local electronic structure and thus improve the performance of a graphene powered device. Therefore, developing a method of graphene production that is low in cost, simple in operation and high in controllability is important. It carries a vital value for the industrial applications of graphene materials in the future. For this study, graphene oxide (GO) and GO/AuNPs (gold nanoparticles) films were prepared using plasma jet printing technology. Then, a dielectric barrier discharge at a sub-atmospheric pressure in nitrogen gas was used to obtain N-doped and reduced graphene oxide (N-rGO). The microstructure of the sample was observed using a scanning electron microscope. Then, the materials were analyzed using X-ray diffraction, Raman spectra, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Finally, the cyclic voltammetry profiles and galvanostatic charge/discharge curves of N-rGO and N-rGO/AuNPs capacitors were tested. Results have indicated that the ultimate materials with a porous structure after the nitrogen plasma treatment presented a pretty good capacitor performance. [ABSTRACT FROM AUTHOR]

Key message: FLO15encodes a plastidic glyoxalase I protein, OsGLYI7, which affects compound starch granule formation and starch synthesis in rice endosperm.Abstract: Starch synthesis in rice (Oryza sativa) endosperm is a sophisticated process, and its underlying molecular machinery still remains to be elucidated. Here, we identified and characterized two allelic rice floury endosperm 15 (flo15) mutants, both with a white-core endosperm. The flo15 grains were characterized by defects in compound starch granule development, along with decreased starch content. Map-based cloning of the flo15 mutants identified mutations in OsGLYI7, which encodes a glyoxalase I (GLYI) involved in methylglyoxal (MG) detoxification. The mutations of FLO15/OsGLYI7 resulted in increased MG content in flo15 developing endosperms. FLO15/OsGLYI7 localizes to the plastids, and the in vitro GLYI activity derived from flo15 was significantly decreased relative to the wild type. Moreover, the expression of starch synthesis-related genes was obviously altered in the flo15 mutants. These findings suggest that FLO15 plays an important role in compound starch granule formation and starch synthesis in rice endosperm. [ABSTRACT FROM AUTHOR]

New channels, new carriers continue to produce new data types, how to deal with complex and volatile multi-source data under IoT big data environment has become an important issue. Information perception is the basic content of the development of things, but also an important means to achieve information perception. Information interaction is the basic condition for the realization of Internet of things, but also the basic technology of interconnection. The information sensing of the Internet of Things is mainly the source of information provided by it, which is also the application base of the Internet of Things itself. To effectively process the IoT data, this paper proposes the multimodal intelligent data analyzing paradigm for Internet of things systems. Our proposed multiple game theory based data analyzing paradigm will guarantee the optimal combination of the mentioned model to construct the effect and robust analyzing pattern. We construct the optimal combination of Bayesian model, Decision Tree, Random Forest, Logistic Regression, SVM, Adaboost, Markov and Neural Network under the multiple game framework. Several new ideas are proposed to enhance the traditional Nash Equilibrium. Performance of the proposed method is validated through the simulation compared with the other state-of-the-art algorithms. [ABSTRACT FROM AUTHOR]

Key message: OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice. Abstract: The chloroplast has its own genetic material and genetic system, but it is also regulated by nuclear-encoded genes. However, little is known about nuclear-plastid regulatory mechanisms underlying early chloroplast biogenesis in rice. In this study, we isolated and characterized a mutant, osppr6, that showed early chloroplast developmental defects leading to albino leaves and seedling death. We found that the osppr6 mutant failed to form thylakoid membranes. Using map-based cloning and complementation tests, we determined that OsPPR6 encoded a new Pentatricopeptide Repeat (PPR) protein localized in plastids. In the osppr6 mutants, mRNA levels of plastidic genes transcribed by the plastid-encoded RNA polymerase decreased, while those of genes transcribed by the nuclear-encoded RNA polymerase increased. Western blot analyses validated these expression results. We further investigated plastidic RNA editing and splicing in the osppr6 mutants and found that the ndhB transcript was mis-edited and the ycf3 transcript was mis-spliced. Therefore, we demonstrate that OsPPR6, a PPR protein, regulates early chloroplast biogenesis and participates in editing of ndhB and splicing of ycf3 transcripts in rice. [ABSTRACT FROM AUTHOR]

Single-molecule nuclear magnetic resonance is a current challenge in the field of magnetic resonance spectroscopy and has important applications in chemical analysis and quantum computing. Through decoherence measurements of nitrogen-vacancy centres under dynamical decoupling control, the sensing of a single 13C nuclear spin at nanometre distance has recently been realized. A further step towards the ultimate goal of structure analysis of single molecules would be the direct measurement of the interactions within single nuclear-spin clusters. Here we sense a single 13C-13C nuclear-spin dimer located about 1 nm from the nitrogen-vacancy centre and characterize the interaction (∼690 Hz) between the two nuclear spins. From the measured interaction we derive the spatial configuration of the dimer with atomic-scale resolution. These results indicate that, in combination with advanced material-surface engineering, central spin decoherence under dynamical decoupling control may be a useful probe for nuclear magnetic resonance single-molecule structure analysis. [ABSTRACT FROM AUTHOR]

Emerging evidence has demonstrated that exposure to anesthetics early in life caused neurohistopathologic changes and persistent behavioral impairments. In this study, a maternal fetal rat model was developed to study the effects of isoflurane exposure during pregnancy on postnatal memory and learning in the offspring. Pregnant rats at gestational day 14 were either exposed to 1.3% isoflurane in a humidified 100% oxygen carrier gas or simply humidified 100% oxygen without any inhalational anesthetic for 2 h every day before delivery. Four weeks later, spatial learning and memory of the offspring were examined using the Morris Water Maze. The expression levels of GAP-43 and NPY in the hippocampal CA1 region of the pups were determined by immunohistochemistry and RT-PCR. Simultaneously, the ultrastructure changes in synapse of the hippocampus were also observed by transmission electron microscopy (TEM). Isoflurane exposure during pregnancy impaired postnatal spatial memory and learning in the offspring as shown by the longer escape latency and the fewer original platform crossings in the Morris Water Maze test. The number and optical densities of GAP-43 and NPY positive cells, as well as the levels of GAP-43 and NPY mRNA, decreased significantly in the hippocampus of isoflurane-exposed pups. Furthermore, TEM studies showed remarkable changes in synaptic ultrastructure of hippocampus. These results indicate that isoflurane exposure during pregnancy could cause postnatal spatial memory and learning impairments in offspring rats, which may be partially explained by the down-regulation of GAP-43 and NPY in the hippocampal area. [ABSTRACT FROM AUTHOR]

The diagnosis and treatment of intussusception is often confusing in infants aged younger than 3 months. This study aimed to discuss the particularity of diagnosis and treatment of intussusception in this age group. From April 1983 to June 2008, 39 infants aged 3 months or younger who had been diagnosed with intussusception were treated and their clinical data were analyzed retrospectively. Of the 39 infants (29 boys and 10 girls), ages ranged from 12 hours to 3 months, with a mean age of 52.6 days. The duration from onset to admission ranged from 7 to 142 hours (mean 39.6 hours). Three infants had intrauterine intussusception and 36 postnatal intussusception. The 3 infants with intrauterine intussusception had typical presentations of complete ileus after birth. Gap type ileal atresia was found in surgery in 2 of the 3 infants and primary anatomosis was performed therapeutically. The other infant was found to have ileal separation and a patent proximal end with diffused meconium peritonitis. The patient died 2 days after primary anastomosis. Most infants with postnatal intussusception had two or more manifestations of the tetralogy, namely intermittent screaming, vomiting, bloody stool, and abdominal mass. In 23 infants who underwent pneumatic reduction, 17 had a successful reduction and 6 converted to open surgery. Surgery was indicated for 19 infants, with maneuver procedure in 14. Meckel’s diverticulum was noted as a leading cause in 2 infants, ileal duplication in 1 with necrosis of intussusceptum, and primary intussusception with lead point necrosis in 2. The 5 infants, on whom segmental resection was performed, underwent primary anastomosis. All infants with postnatal intussusception had a smooth recovery. Infants aged 3 months or younger may suffer from intussusception and most of them present with typical symptoms. Early diagnosis and treatment are needed for a good prognosis. Intrauterine intussusception may be an etiological factor for ileal atresia. [ABSTRACT FROM AUTHOR]

In this investigation, mesoporous TiO-SiO composites with different Ti/Si molecular ratios were synthesized by one pot method. Apart from the characterization by small angle X-ray diffraction (SAXRD), transmission electron microscope (TEM) and N absorption-desorption isotherms, these mesoporous materials were used in the adsorption and enrich of biological molecules. The results demonstrate that TiO-SiO composites possess the ordered mesoporous structure with uniform pore. Bovine hemoglobin (BHb) can be immobilized within the mesopore of the composite and the immobilization amount increases with the Ti/Si molecular ratio. It is speculated that the strong electrostatic interaction between biomolecule and the inner surface of mesoporous materials enhances the adsorption of BHb. Moreover, our investigation confirms that the absorbed BHb still effectively retains its bioactivity, which is very significant for the potential application of this hybrid material in biocatalysis. [ABSTRACT FROM AUTHOR]

Hepatitis C virus (HCV) infection greatly increases the risk of nephropathy. In this observational study, we aimed to explore the relationship between viral hepatitis infection and chronic kidney disease (CKD), identify risk factors, and determine the effect of antiviral treatment on CKD in Chinese patients with chronic HCV infection. A total of 2,435 study subjects were enrolled and divided into four groups: the HCV infection, HBV infection, HBV and HCV co-infection, and uninfected control groups. Of these, 207 patients with chronic hepatitis C (CHC) were given standard dual therapy [subcutaneous injection of recombinant interferon (IFN)-α2b and oral ribavirin (RBV)] for 48 weeks. We found that the prevalence of CKD gradually increased with age in all groups and was significantly increased in patients 60 years or older. Multivariate logistic regression analyses showed that persistent HCV infection was significantly associated with CKD [odds ratio (OR), 1.33; 95% confidence interval (CI), 1.06-1.66; P = 0.013], whereas there was no significant link between CKD and spontaneous HCV clearance (OR, 1.23; 95% CI, 0.79-1.90; P = 0.364), HBV infection (OR, 0.73; 95% CI, 0.44-1.19; P = 0.201), or HBV/HCV co-infection (OR, 1.40; 95% CI, 0.81-2.40; P = 0.234). Notably, after anti-HCV therapy, the serum creatinine concentration was significantly decreased (76.0, 75.5-79.4 μmol/L) from the pretreatment level (95.0, 93.0-97.2 μmol/L), both in patients who showed an end of treatment virological response (ETVR) and those who did not (P < 0.001). Also, in both the ETVR and non-ETVR groups, the percentages of patients with an estimated glomerular filtration rate (eGFR) ≥90 ml/min/1.73 m2 increased significantly (P < 0.001), whereas the percentages of those with an eGFR <60 ml/min/1.73 m2 significantly decreased (P < 0.001). In conclusion, persistent HCV infection was independently associated with CKD, and antiviral treatment with IFN plus RBV can improve renal function and reverse CKD in HCV-infected patients. [ABSTRACT FROM AUTHOR]

Lipotoxicity has been implicated in pancreatic β-cell dysfunction in type 2 diabetes, but the exact mechanisms remain unknown. The current study explored the role of the endoplasmic reticulum (ER) stress pathway in cholesterol-induced lipotoxicity. Two different insulinoma cell lines were treated with cholesterol with or without inhibitors. ER stress-associated proteins glucose-regulated protein (GRP) 78, activating transcription factor (ATF) 4 and C/EBP homologous protein (CHOP), as was phosphorylation of eukaryotic initiation factor (EIF) 2α, were all up-regulated by cholesterol. Cholesterol also up-regulated microtubule-associated protein 1 light chain 3 (LC3)-II and stimulated the formation of autophagic vacuoles and LC3-II aggregates. Cholesterol-induced autophagy and cell injuries were suppressed by pretreatment with the ER stress inhibitor 4-phenylbutyrate (4-PBA). Pretreatment with autophagy inhibitors E-64d/pepstatin A increased ER stress-induced cell injuries as indicated by increased cell apoptosis and decreased insulin secretion. These results suggest that cholesterol treatment induces apoptosis and dysfunction of β-cells, and enhances autophagy through activation of the ER stress pathway. More importantly, autophagy induced by cholesterol may protect β-cells against ER stress-associated cell damages. [ABSTRACT FROM AUTHOR]