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A method combining solid-phase extraction and high-performance liquid chromatography (SPE-HPLC) has been developed for analysis of ten rare ginsenosides including 20( R)-Rh, 20( S)-Rh, 20( R)-Rg, 20( S)-Rg, Rg, Rk, Rg, F, Rk, and Rh in three kinds of injection (Shenfu, Shenmai, and Shengmai). Waters Oasis HLB SPE columns were used to clean and enrich the sample. An Eclipse XDB-C column was used to separate the analytes, with water and acetonitrile as mobile phase components under gradient elution conditions at 30 °C. There were good linear correlations between the signals measured and the concentrations of the ten analytes ( r ≥ 0.9996). Intraday and interday precision were both better than 2.19% and average recovery ranged from 95.25 to 108.83%. The proposed method was successfully applied to determination of the ten analytes in different batches of the injections and the results indicated the method is simple, rapid, and accurate. The proposed method could be used for quality control during manufacture of the injections. [ABSTRACT FROM AUTHOR]

Nine new bicyclic cembranoids, sarcophytrols M-U(1-9), were isolated from the South China Sea soft coral Sarcophyton trocheliophorum as minor components, along with one known related cembranoid 10. Their structures were elucidated by detailed spectroscopic analysis and chemical conversion. The chemical structures of these metabolites are characterized by the different patterns of the additional cyclization within the 14-member skeleton, which leading to the formation of furan, pyran, oxepane, and peroxyl rings, respectively. Among them, sarcophytrols R and S(6 and 7) share a rare decaryiol skeleton with an unusual C12/C15 cyclization. In addition, the absolute configurations of sarcophytrols M and T(1 and 8) were determined by the modified Mosher's method. The research of these new secondary metabolites provided a further understanding of the diversity of cyclized cembranoids from the title species. [ABSTRACT FROM AUTHOR]