1. Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals.
- Author
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Luo, Lingjie, Liang, Wenhua, Pang, Jianfeng, Xu, Gang, Chen, Yingying, Guo, Xinrong, Wang, Xin, Zhao, Yi, Lai, Yangdian, Liu, Yang, Li, Bin, Su, Bing, Zhang, Shuye, Baniyash, Michal, Shen, Lei, Chen, Lei, Ling, Yun, Wang, Ying, Liang, Qiming, and Lu, Hongzhou
- Subjects
COVID-19 ,CELL physiology ,T cells ,SARS-CoV-2 ,BLOOD cells ,VACCINE development - Abstract
SARS-CoV-2 outbreak has been declared by World Health Organization as a worldwide pandemic. However, there are many unknowns about the antigen-specific T-cell-mediated immune responses to SARS-CoV-2 infection. Here, we present both single-cell TCR-seq and RNA-seq to analyze the dynamics of TCR repertoire and immune metabolic functions of blood T cells collected from recently discharged COVID-19 patients. We found that while the diversity of TCR repertoire was increased in discharged patients, it returned to basal level ~1 week after becoming virus-free. The dynamics of T cell repertoire correlated with a profound shift of gene signatures from antiviral response to metabolism adaptation. We also demonstrated that the top expanded T cell clones (~10% of total T cells) display the key anti-viral features in CD8
+ T cells, confirming a critical role of antigen-specific T cells in fighting against SARS-CoV-2. Our work provides a basis for further analysis of adaptive immunity in COVID-19 patients, and also has implications in developing a T-cell-based vaccine for SARS-CoV-2. [ABSTRACT FROM AUTHOR]- Published
- 2021
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