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Context: Elucidating how demography and contemporary landscape features regulate functional connectivity is crucial to implementing effective conservation strategies. We assessed the impacts of landscape features on the genetic variation of a locally endangered carnivore, the leopard cat (Prionailurus bengalensis) in Taiwan. Objectives: We aim to evaluate the association between genetic structure and landscape features. We further predicted the changes in genetic diversity and suitable habitats in the future. Methods: We genotyped 184 leopard cats in western Taiwan using 12 nuclear microsatellites and a mitochondrial marker. We applied a landscape optimization procedure with two genetic distances to identify major genetic barriers and employed ecological niche modeling to predict the future distribution of the leopard cat. Results: Bayesian demographic inferences revealed a dramatic population decline for all leopard cat populations in Taiwan. Genetic clustering and resistance surface modeling supported that the population connectivity was influenced by highways and high elevation. Niche modeling indicated low temperature was one of the primary factors limiting the occurrence of leopard cats that may inhibit their movement in high elevations. We predicted the suitable habitats of leopard cats would shrink northward and towards higher altitudes with rugged topography in response to global warming. Conclusions: Our study provided genetic evidence that leopard cats in Taiwan had undergone a dramatic population decline that may be associated with anthropogenic impacts. We also inferred the anthropogenic linear feature compromised the connectivity and persistence of leopard cats in human-mediated landscapes. Our finding serves as a model for landscape genetic studies of island carnivores in subtropical regions. [ABSTRACT FROM AUTHOR]

With the expected rise of patients with osteoporosis-induced fractures, it has become increasingly urgent to design and use specialized health education materials to ease pain and improve bodily functions. This study designed web-based app for pain relief instructions for women with spinal compression fractures. An observational study was conducted at an educational hospital in northern Taiwan between October 2018 and September 2019. Using random assignment based on their presentation order, we divided patients into an experimental (n = 87) and control (n = 84) group. The experimental and control groups received web app healthcare instructions for relieving back pain and regular nursing care instructions, respectively. Taiwanese versions of the Brief Pain Inventory and 36-Item Short Form Health Survey were used to collect data. We collected patients' pain rating data the day before, one month after, and three months after providing them with the instructions. Pain intensity differed significantly between the two groups, and it interacted with time. Pain impact did not significantly differ between groups; however, it interacted with time. Relieving back pain is a primary concern for those affected by it. This study presents a consistent, easy-to-use instructional healthcare web-based app that may provide knowledge of pain relief and reduce mobility impairments. Trial registration: first registration on 18/05/2022, NCT05386212. [ABSTRACT FROM AUTHOR]

The relationship between Helicobacter pylori infection and rheumatoid arthritis has been investigated, but the results remain controversial. This study aims to determine the association between the two diseases via a 17-year retrospective cohort study. Using the National Health Insurance Research Database, a nationwide population based in Taiwan, we identified 97,533 individuals with H. pylori infection and matched controls between 2000 and 2017 using propensity score matching at a 1:1 ratio. The adjusted hazard ratio of rheumatoid arthritis was determined by multiple Cox regression. The incidence rate of rheumatoid arthritis was 1.28 per 10,000 person-months in the H. pylori cohort, with a higher risk compared to the control group. In the < 30 years old subgroup, the risk was highest, especially in women < 30 years old with H. pylori infection. Patients with < 1 year follow-up showed 1.58 times higher susceptibility to rheumatoid arthritis. Individuals with follow-ups of 1–5 years and over 5 years demonstrated 1.43 and 1.44 times higher risks of rheumatoid arthritis, respectively. Our study showed H. pylori infection was associated with the development of rheumatoid arthritis. Clinicians should note higher risk, especially < 30 years old. More research needed to understand underlying mechanism. [ABSTRACT FROM AUTHOR]

Lanthanide doping is an effective strategy for modulating the emission of emitters. Herein, by changing the cluster composition to control the energy transfer pathway, the application potential of high-nuclearity lanthanide cluster (HLC) as white-light emitter has been confirmed for the first time. Specifically, by precisely controlling the proportion of GdIII, TbIII, and EuIII ions in reactants, we obtained a spherical heterotrimetallic nanocluster Gd10Tb12Eu10, a white-light emitter with quantum yield (QY) of 12.58% and lifetime of 327.14 µs. High-resolution electrospray ionization mass spectrometry (HRESI-MS) demonstrates that homometallic nanoclusters Ln32 (Ln = Gd, Tb, and Eu) are tetracationic clusters and are highly stable in solution. The peripheral dense organic ligands provide a protective layer for the cluster core, which improves the stability of Ln32 in aqueous solution, avoids the contact between metal centers and bioactive molecules, and greatly reduces the biological toxicity. In cell imaging experiments, cationic clusters Ln32 are mainly localized on the cell membrane with negative charge distribution. As far as we know, this is the first time that spherical lanthanide nanoclusters have been used for membrane imaging of living cell, opening the door for the application of HLCs in biological imaging. [ABSTRACT FROM AUTHOR]

Purpose: It is commonly recommended that tooth extraction should be performed prior to radiotherapy (RT) in patients with head neck cancer to prevent osteoradionecrosis (ORN). However, doctors still occasionally encounter patients who require tooth extraction during RT. This study aimed to determine the risk of ORN in patients who undergo tooth extraction during RT. Methods: Data were collected from Taiwan's National Health Insurance Research Database. We retrospectively enrolled 24,412 patients with head and neck cancer treated with radiotherapy between 2011 and 2017. The associations between ORN and demographic characteristics, timing of tooth extraction, and treatments were examined using univariate and multivariable Cox proportional hazards regression models. Results: A total of 24,412 head and neck cancer patients were enrolled; 133 patients underwent tooth extraction during RT and 24,279 patients did not undergo tooth extraction during RT. Tooth extraction during RT was not associated with a significantly higher risk of ORN (hazard ratio [HR] = 1.303, P = 0.4862). Tumor site, RT dose ≥ 60 Gy, age < 55 y/o, mandibulectomy, chronic periodontitis, and chemotherapy were significantly associated with a higher risk of ORN. Conclusion: The risk of ORN in head and neck cancer is not significantly different between patients who undergo tooth extraction during RT and patients who do not undergo tooth extraction during RT. [ABSTRACT FROM AUTHOR]

Phytoreclamation is the intervention of plants to improve degraded soil quality, changing soil biotic and abiotic properties. Many studies have focused on microbial composition and bioactivity, but few explored the changes in phylogenetic assemblages of soil microbiota after phytoreclamation. This study compared microbiomes of bare land to those of planted soils and investigated how the rhizosphere environment affects microbial assemblages from monocot Poa pratensis and eudicot Dianthus plumarius plantings using 16S rRNA metabarcoding. The results showed that the biotic susceptibility of soil to the rhizosphere environment was higher than that of the abiotic. A noticeable change was in some soil physicochemical properties like Na, P, Zn, Cu, C, and sand-to-silt proportion before and after phytoreclamation, but not between the rhizosphere and bulk soil of plantings. Contrastingly, microbial composition and diversity were significantly affected by both turfing and rhizosphere effects and were more susceptible to differences in turfing or not than in planting species. In the turfgrass, the microbiome differences between plants were greater in the rhizosphere than in the surrounding bulk soil, indicating the proximal influence of root exudates. We also found that the main abiotic factors that influenced microbial composition were Na, Zn, NOx, N, and S; as for the phylogenetic assemblages, were by K levels and the increase of silt. Turfgrass decomposes soil aggregates and changes the physicochemical properties, thereby evens the phylogenetic clustering of the soil microbial community. We demonstrated that the deterministic process affects the microbial assemblage and acts as a selective agent of the soil microbiota in fundamental and realized niches. Phytoreclamation may lead to abiotic soil changes that reallocate resources to microbes. This could affect the phylogeny of the microbial assemblages and increase microbial richness. [ABSTRACT FROM AUTHOR]

We develop an overlapping-generations framework of education-based migration that takes place prior to labor-market participation and explore its role for economic development, urbanization and workforce composition. We show that education-based and work-based migration are substitutes and the equilibrium outcome depends crucially on children's talent distribution, college costs and selectiveness, urban job opportunities, and migration barriers. We establish conflicting partial- and general-equilibrium effects at work for comparative statics, and examine their locational as well as macroeconomic implications for assessing education and migration policies. Applying our model to fit the data from China over 1980–2007, we find that, although education-based migration only amounts to one-fifth of that of work-based migration, it contributes more to per capita output growth than work-based migration owing to its high-skilled nature. Moreover, the abolishment of education-based migration policy and the relaxation of the work-based migration are found to have limited effects on per capita output and urbanization. [ABSTRACT FROM AUTHOR]

The efficacy of urban teachers has been an important concern of school administrators. This study explores how teacher's intrinsic and extrinsic work motivation, relationships and school contexts affect teacher efficacy. The sources were surveys collected from elementary school teachers in Taipei City, Taiwan and data and demographic information collected from Education Statistical Data in Taipei City Government, Taiwan. The results of multilevel modeling indicated that school-level principal leadership and work environment enhance teaching. For teacher-level variables, only parent–teacher relationship has a significant influence on teacher efficacy. The implications and research limitations are discussed. [ABSTRACT FROM AUTHOR]

Heloniadeae (Melanthiaceae) presents an East Asia–North America disjunct distribution. Different molecular and morphological data nevertheless support the tribe as a monophyletic group. However, their phylogenetic relationships and biogeographic history, together with the character evolution, are not clear. Therefore, we constructed a Bayesian phylogenetic tree for Heloniadeae using cpDNA and inferred the historical biogeography and floral character evolution. The results revealed that Heloniadeae was distributed in high-latitudes of East Asia and North America, originating since 22.2 mya. The East Asia clade migrated into southwest China, and subsequently colonized the Korean Peninsula, Taiwan, the Ryukyus, and spread northward to Japan and southern Sakhalin. The evolution of the inflorescence and number of flowers were phylogenetically conserved, associated with the historical biogeography of Heloniadeae. The inflorescences transferred from raceme to sub-umbel, and the number of flowers decreased during the dispersal process, which may be accompanied by changes in the breeding system. Besides, the anthesis period was more affected by the habitat environment than phylogenetic constraints. The flowering temperature of was below 20 °C in most species, except H. kawanoi. Such a low temperature might not be conductive to pollinator activities, but it could be compensated by sustaining seed production with long-lasting flowers. [ABSTRACT FROM AUTHOR]

Purpose: The association between the tumor subsites of the oral cavity and the risk of osteoradionecrosis of the jaw (ORNJ) remains unclear. We study the correlation between oral cavity tumor subsites and the risk of ORNJ in a nationwide population-based database. Methods: We enrolled 16,701 adult patients with oral cavity cancers who were treated with radiotherapy between 2000 and 2013. The subsites of the oral tumor, treatments of oral cavity cancers, and the timing of tooth extraction were examined for their association with ORNJ in oral cancer patients. Results: 903 patients (5.40%) developed ORNJ. Of the relevant variables, pre-RT mandible surgery, tooth extraction either before or after RT, and tumor sites were associated with the risk of ORNJ. The adjusted HRs for ORNJ in the mouth floor, gums, retromolar, and buccal cancer were 2.056 (1.490–2.837), 1.909 (1.552–2.349), 1.683 (1.105–2.562), and 1.303 (1.111–1.528), respectively, compared with the risk of tongue cancer. There was no significant difference in the risk of ORNJ between the pre-RT extraction group, the during-RT extraction group, and the post-RT extraction (less than 6 months) group; the post-RT extraction (more than 6 months) group had a significantly higher risk of ORNJ. Conclusions: This study demonstrated that oral cavity tumor subsite is an independent risk factor of ORNJ after RT. Post-RT extraction (less than 6 months) group did not carry a significantly higher risk of ORNJ compared with pre-RT extraction group or during RT extraction group. [ABSTRACT FROM AUTHOR]

The limited availability of nasopharyngeal carcinoma-related progression biomarker array kits that offer physicians comprehensive information is disadvantageous for monitoring cancer progression. To develop a biomarker array kit, systematic identification and differentiation of a large number of distinct molecular surface-enhanced Raman scattering (SERS) reporters with high spectral temporal resolution is a major challenge. To address this unmet need, we use the chemistry of metal carbonyls to construct a series of unique SERS reporters with the potential to provide logical and highly multiplex information during testing. In this study, we report that geometric control over metal carbonyls on nanotags can produce 14 distinct barcodes that can be decoded unambiguously using commercial Raman spectroscopy. These metal carbonyl nanobarcodes are tested on human blood samples and show strong sensitivity (0.07 ng/mL limit of detection, average CV of 6.1% and >92% degree of recovery) and multiplexing capabilities for MMPs. SERS assays have potential for multiplexed detection of biomarkers but differentiation of SERS tags remains a challenge. Here, the authors report the creation of 14 distinct geometrically controlled metal carbonyl tags and demonstrate multiplexed detection of nasopharyngeal carcinoma biomarkers from patient blood. [ABSTRACT FROM AUTHOR]

Few studies have demonstrated an association of sarcopenia-associated body measurements with chronic diseases through a comprehensive methodology. This study aims to examine the relationship between sarcopenia-associated body measurements and chronic diseases. This is a cohort study. We recruited 316 community dwellers, including 76 patients with sarcopenia and 240 controls, and obtained their body measurements associated with sarcopenia. We collected three-dimensional anthropometric body-surface measurements from 11,158 participants during 2000–2008 and followed up this cohort for 15 years to examine the association of these measurements with the risk of chronic diseases such as hypertension, type 2 diabetes mellitus (T2DM), heart disease, and nephrotic syndrome. Univariate analysis, canonical correlation, and Cox regression analysis were performed to explore the associations. Decreased waist width, upper left arm circumference, and left thigh circumference were significantly associated with sarcopenia. The adverse body measure score (ABMS) was derived by combining significant measurements, namely left upper arm circumference, waist width, and left thigh circumference, and used to predict the risk of hypertension, T2DM, heart diseases, and nephrotic syndrome. A positive association was observed between the ABMS and chronic diseases. Considering the first quartile of the ABMS as a reference, we determined hazard ratios of 2.259, 2.495, 1.332, and 1.595 for hypertension, T2DM, heart disease, and nephrotic syndrome, respectively, in the fourth quartile. Chronic diseases were more strongly associated with the ABMS than with sarcopenia-related body measurements alone. A high ABMS, which includes higher upper arm circumference, higher waist width, and lower thigh circumference, can significantly predict chronic diseases. [ABSTRACT FROM AUTHOR]

Amaranthus tricolor L., a vegetable Amaranthus species, is an economically important crop containing large amounts of betalains. Betalains are natural antioxidants and can be classified into betacyanins and betaxanthins, with red and yellow colors, respectively. A. tricolor cultivars with varying betalain contents, leading to striking red to green coloration, have been commercially produced. However, the molecular differences underlying betalain biosynthesis in various cultivars of A. tricolor remain largely unknown. In this study, A. tricolor cultivars with different colors were chosen for comparative transcriptome analysis. The elevated expression of AmCYP76AD1 in a red-leaf cultivar of A. tricolor was proposed to play a key role in producing red betalain pigments. The functions of AmCYP76AD1, AmDODAα1, AmDODAα2, and AmcDOPA5GT were also characterized through the heterologous engineering of betalain pigments in Nicotiana benthamiana. Moreover, high and low L-DOPA 4,5-dioxygenase activities of AmDODAα1 and AmDODAα2, respectively, were confirmed through in vitro enzymatic assays. Thus, comparative transcriptome analysis combined with functional and enzymatic studies allowed the construction of a core betalain biosynthesis pathway of A. tricolor. These results not only provide novel insights into betalain biosynthesis and evolution in A. tricolor but also provide a basal framework for examining genes related to betalain biosynthesis among different species of Amaranthaceae. [ABSTRACT FROM AUTHOR]

The benefits of self-monitoring of blood glucose (SMBG) on glycemic control among type 2 diabetes (T2DM) patients not receiving insulin remains controversial. This study aimed to examine the association between SMBG and glycemic control in these patients. This retrospective longitudinal study enrolled 4987 eligible patients from a medical center in Taiwan. Data were collected from electronic medical records at 0 (baseline), 3, 6, 9, and 12 (end-point) months after enrollment. Patients were assigned to the early SMBG group or to the non-user group depending on whether they performed SMBG at baseline. Differences in glycated hemoglobin (HbA1c) reduction between groups at each time-point were assessed using SMBG group-by-time interaction in generalized estimating equations models, which were established using backward elimination method for multivariate regression analysis. Subgroup analyses for patients using non-insulin and insulin secretagogues were performed additionally. The estimated maximal difference in HbA1c reduction between groups (early SMBG users vs. non-users) was 0.55% at 3 months. Subgroup analyses showed maximal differences of 0.61% and 0.52% at 3 months in the non-insulin and insulin secretagogues groups, respectively. SMBG group-by-time interaction was statistically significant at 3 months and lasted for 12 months. The finding suggests that performing SMBG at disease onset was positively associated with better glycemic control in newly diagnosed non-insulin-treated T2DM patients, regardless whether non-insulin secretagogues or insulin secretagogues were used. [ABSTRACT FROM AUTHOR]

During the last two decades, economic studies on happiness have grown rapidly in particular, studies on the effect of income on happiness. Ng (Pac Econ Rev 7(1):51–63, 2002) has highlighted the East-Asian happiness gap. The East Asian countries, China, Hong Kong, Taiwan, Korea, Japan, Singapore, are performing well economically, however, performing poorly in happiness. Societal values have been suggested to be the potential explanation of this happiness gap. Nevertheless, the effects of societal values on happiness are yet to be explored fully. This paper aims to estimate the effect of income on happiness and examine the moderating effect of societal values in the context of the East-Asian happiness gap using the World Values Survey (WVS) data. The WVS (waves 6, 2010–2014) consists of nationally representative sample of 14,447 respondents from the various East and South Asian countries. It provides measurements of societal values, subjective well-being and other socio-demographic variables including income. We found that the effect of income on happiness is the lowest (and insignificant) in Thailand and Philippines; and the highest (and strongly significant) in South Korea and Taiwan. The effect of income becomes insignificant once it is moderated by the societal values. Societal values matter to explain the East-Asian happiness gap and might refute the relevance of Easterlin paradox. [ABSTRACT FROM AUTHOR]

Cycas taitungensis and its sister species, C. revoluta (Cycas sect. Asiorientalis), are distributed peripatrically. Previous studies have revealed their phylogeographic pattern and, based on certain plastid DNA markers, have established that they diverged recently. However, the speciation process involving the genomic divergence has not been well clarified. In this study, based on their geographical distribution and estimates of their genetic diversity, two speciation models are proposed: (1) the founder and (2) the bottleneck speciation mode. Using approximate Bayesian computation (ABC) in combination with their estimated genetic diversity and population structure, we infer that the ancestral C. taitungensis populations diverged and southward colonized southeastern Taiwan from ancestral C. revoluta since the middle Pleistocene. These founders preserved ancestral polymorphism, resulting in small differences in genetic diversity and effective population size (Ne), despite a large difference in census population size (Nc) between two species. Based on this case of island cycad species offshore of continental Asia, this study provides new insight into how the speciation process influences the genetic diversity pattern of species with small Nc and Ne. [ABSTRACT FROM AUTHOR]

One of the most significant challenges in boron neutron capture therapy (BNCT) is to have an ideal boron delivery agent which can deliver sufficient numbers of boron atoms to designated tumor cells. In this work, mild synthetic conditions for synthesis of dipeptidyl multiple boron-containing analogs under microwave-assisted condition were investigated. The results showed that the reaction generally took place at 50 °C, but higher reaction temperature was required when a fluorinated building block was used. The resulting peptidyl skeletons generated by Ugi four-component reaction resemble basic cell metabolites and could potentially be used as alternative replacements for current boron carrier agents in BNCT. [ABSTRACT FROM AUTHOR]

The composition of the soil microbiome is influenced by environmental (abiotic) variables and biological interactions (biotic factors). To determine whether the aboveground vegetation and soil physicochemical properties were the main determinant of beta-diversity and biological interaction of soil microbial community, we sampled soils from the temperate coniferous forest and grassland. Clustering of operational taxonomic units was conducted using 16S rRNA gene. We found that the microbial composition of the rhizospheres, in which root exudates influence the microbial environment, show lower alpha-diversity than that of nonroot soils. The nonsignificant rhizosphere effect suggested other undetermined factors or stochastic processes accounted for microbial diversity in the rhizosphere. More significant microbe-microbe interactions were observed in forest and rhizosphere soils relative to the grassland soils. The elevated number of positive correlations for relative abundances in forest soil implied beneficial associations being common among bacteria, in particular within the rhizosphere environment. The particular soil properties generated by root exudates also alter the physicochemical properties of soil such as K and pH value, and might in turn favor the adoption of teamwork-cooperation strategies for microbe-microbe interactions, represented as large clusters of positive associations among bacterial taxa. Specific biological interactions differentiated the microbiomes within forest soils. Thus, the environmental selection pressure of aboveground vegetation accounts for differences between soil microbiomes while biotic factors are responsible for fine-scale differences of the microbial community in forest soils. [ABSTRACT FROM AUTHOR]

Background: Molecular identification based on microsatellite loci is an important technology to improve the commercial breeding of the moth orchid. There are more than 30,000 cultivars have been enrolled at the Royal Horticultural Society (RHS). In this study, genomic microsatellite primer sets were developed from Phalaenopsis aphrodite subsp. formosana to further examine the transferability of across 21 Phalaenopsis species. Methods and results: Twenty-eight polymorphic microsatellite markers were obtained using the magnetic bead enrichment method, with high transferability of the 21 species of the genus Phalaenopsis, especially in the subgenus Phalaenopsis. The 28 newly developed polymorphic microsatellite markers with high polymorphism information content values. The best and second fit grouping ( K) are inferred as two and four by the ΔK evaluation in the assignment test. This result indicates that these microsatellite markers are discernible to subgenus Phalaenopsis. Conclusions: Our results indicate that these new microsatellite markers are useful for delimiting species within genus Phalaenopsis. As expected, the genetic relationships between species of subgenus Phalaenopsis can be well distinguished based on the assignment test. These molecular markers could apply to assess the paternity of Phalaenopsis as well as investigating hybridization among species of genus Phalaenopsis. [ABSTRACT FROM AUTHOR]

Key message: The homologous genes OsbHLH068 and AtbHLH112 have partially redundant functions in the regulation of the salt stress response but opposite functions to control flowering in Arabidopsis. Abstract: The transcription factor (TF) basic/Helix-Loop-Helix (bHLH) is important for plant growth, development, and stress responses. OsbHLH068, which is a homologous gene of AtbHLH112 that is up-regulated under drought and salt stresses, as indicated by previous microarray data analysis. However, the intrinsic function of OsbHLH068 remains unknown. In the present study, we characterized the function and compared the role of OsbHLH068 with that of its homolog, AtbHLH112. Histochemical GUS staining indicated that OsbHLH068 and AtbHLH112 share a similar expression pattern in transgenic Arabidopsis during the juvenile-to-adult phase transition. Heterologous overexpression of OsbHLH068 in Arabidopsis delays seed germination, decreases salt-induced HO accumulation, and promotes root elongation, whereas AtbHLH112 knock-out mutant displays an opposite phenotype. Both OsbHLH068-overexpressing transgenic Arabidopsis seedlings and the Atbhlh112 mutant display a late-flowering phenotype. Moreover, the expression of OsbHLH068-GFP driven by an AtbHLH112 promoter can compensate for the germination deficiency in the Atbhlh112 mutant, but the delayed-flowering phenotype tends to be more severe. Further analysis by microarray and qPCR indicated that the expression of FT is down-regulated in both OsbHLH068-overexpressing Arabidopsis plants and Atbhlh112 mutant plants, whereas SOC1 but not FT is highly expressed in AtbHLH112-overexpressing Arabidopsis plants. A comparative transcriptomic analysis also showed that several stress-responsive genes, such as AtERF15 and AtPUB23, were affected in both OsbHLH068- and AtbHLH112-overexpressing transgenic Arabidopsis plants. Thus, we propose that OsbHLH068 and AtbHLH112 share partially redundant functions in the regulation of abiotic stress responses but have opposite functions to control flowering in Arabidopsis, presumably due to the evolutionary functional divergence of homolog-encoded proteins. [ABSTRACT FROM AUTHOR]

Solvothermal reaction of Zn(NO), 4-(1 H-pyrazol-4-yl)pyridine (Hpypz) and 1,3,5-benzenetricarboxylic acid (Hbtc) in N,N-dimethylacetamide (DMA) produced a new porous coordination polymer [Zn(pypz)(btc)] ( 1). Single-crystal X-ray diffraction study of 1 showed that deprotonated pypz ligands served as 1,2,4-triazolate-like ligands, linking Zn(II) ions to form porous two-dimensional (2-D) sql-a layers {Zn(pypz)}, which were further connected by eight-legged pillars {Zn(btc)(HO)} based on the typical paddlewheel dinuclear Zn2(RCOO)(HO) cluster to form a novel type of non-interpenetrated pillared-layer framework with 3-D intersecting pore system and large pore volume. Gas sorption measurements revealed that 1 possesses large BET surface area of 2061 m g and very high methane total uptake of 245 cm(STP) cm at 298 K and 65 bar. [ABSTRACT FROM AUTHOR]

Background: The influence of Mycoplasma pneumoniae (MP) infection on bronchiolitis remains unclear. Additionally, reports on the efficacies of leukotriene receptor antagonists in the treatment of bronchiolitis have been inconclusive. Methods: Children with respiratory syncytial virus (RSV)-induced bronchiolitis were divided into two groups: RSV+MP group and RSV group. Each group was randomly divided into two subgroups: one received routine and placebo treatment, while the other received routine and montelukast treatment for 9 months. The cumulative numbers of wheezing episodes and recurrent respiratory tract infections were recorded. Blood parameters were determined. Results: Patients in the RSV+MP group exhibited an older average age, fever, more frequent flaky and patchy shadows in chest X-rays, more frequent extrapulmonary manifestations, and longer hospital stays compared with patients in the RSV group. Additionally, higher baseline blood eosinophil counts, eosinophil cationic protein (ECP), total immunoglobulin E (IgE), interleukin (IL)-4, IL-5, IL-4/interferon-γ ratios, leukotriene (LT) B, and LTC, and lower baseline lipoxin A4 (LXA)/LTB ratios were observed in the RSV+MP group compared with the RSV group. Montelukast treatment decreased the cumulative numbers of recurrent wheezing episodes and recurrent respiratory tract infections at 9 and 12 months. This efficacy may be related to the montelukast-induced reductions in peripheral eosinophil counts, ECP and total IgE, as well as the montelukast-dependent recovery in T helper (Th) 1/Th2 balance and LXA/LTB ratios in children with bronchiolitis. Conclusions: RSV bronchiolitis with MP infection was associated with clinical and laboratory features that differed from those of RSV bronchiolitis without MP infection. Add-on therapy with montelukast for 9 months was beneficial for children with bronchiolitis at 9 and 12 months after the initiation of treatment. [ABSTRACT FROM AUTHOR]

Mercury and polychlorinated dibenzo-pdioxins and dibenzofurans (PCDD/Fs) accumulate in organisms through food webs and exert potentially toxic effects on aquatic organisms and humans. This study examined the levels of mercury and PCDD/Fs in organisms and sediment samples collected from a saltwater pond at the An-Shun site, a chloralkali factory that shut down in Tainan City, Taiwan. It was also a pentachlorophenol production plant. After the factories were shut down in the 1980s, mercury and PCDD/Fs contamination remained, posing severe health hazards. The correlation between PCDD/Fs congener accumulation patterns in distinct fish organs and the sediment was evaluated. Mercury and PCDD/Fs levels in all the fish samples exceeded food safety limits, and the concentrations of mercury and PCDD/Fs in each species were closely correlated (n=12, Spearman’s rank correlation [R]=0.811, p<0.01). The mercury concentrations were positively but non-significantly correlated with the weight (n=11, R=0.741, p<0.01) and length (n=11, R=0.618, p<0.05) of the species. The fish likely accumulated the contaminants through ingestion of other organisms or the sediment. However, after the pollutants entered a fish, they exhibited distinct accumulation patterns because of their differing chemical properties. Specifically, the mercury concentration was correlated with organism weight and length, whereas the PCDD/Fs concentration was associated with organ lipid content. The study results are valuable for assessing the health risks associated with ingesting mercury- and PCFF/F-contaminated seafood from the study site. [ABSTRACT FROM AUTHOR]

The purpose of this paper is to investigate the influence of objective air quality on individual life satisfaction (LS) through the moderating effect of perceived air quality on the latter. Unlike previous studies, we incorporates both of the data from the 2010 Taiwan Social Change Survey and the monthly mean of Pollutant Standards Index (PSI) from fixed air quality monitoring stations to understand their association with individual well-being. A two-stage estimation approach is employed, in which individual perceived subjective air quality level is employed as an explanatory variable in LS regression models. The results show that the objective measure of air quality, PSI, is significantly associated with the perceived air quality and with LS, respectively. When the endogeneity of perceived air quality is considered in the relationship between objective air quality and life satisfaction, the results indicate that the perceived air quality has a positive influence on LS, while the effect of objective measure of air quality on the latter becomes insignificant. In sum, the objective air quality has an indirect effect, but no direct effect, on LS. Furthermore, individual's age, gender, health, and variables of environmental experience and behaviors are found to be significant determinants of LS. Discussions on the findings are provided. [ABSTRACT FROM AUTHOR]

As has occurred in many developed countries, Taiwanese women have gradually become more highly educated and increasingly participated in the labor market. Consequently, Taiwanese women are becoming more independent and are choosing not to get married. As a result, more Taiwanese men have married women from mainland China and Southeast Asian countries. This study contributes to this interesting topic by investigating the happiness of immigrant wives in Taiwan. Special attention is paid to the role of bargaining power plays in immigrant wives' happiness. A nationwide survey of 10,153 immigrant wives in 2008 and a two-stage econometric regression framework were used in this study; the results show that the socio-demographic characteristics of the immigrant wives and their Taiwanese husbands are significantly associated with the immigrant wives' bargaining power and happiness. Immigrant wives with greater bargaining power are happier. [ABSTRACT FROM AUTHOR]

The single-item measure of general happiness has been widely used in questionnaires due to the advantages of easy implementation in surveys for comparison across time and culture. The balanced response scale that includes equal positive and negative response categories based on Likert-type response format has been commonly applied. However, the possibility of using an unbalanced response scale for happiness, for instance, more choices on the happy side, has not been fully examined. This study aims to explore the optimal number of response categories and the corresponding labels for general happiness by using telephone survey data in Taiwan. Six types of response scales with different combinations of response number and response labels were examined to distinguish both the intensity and direction of responses. A completely randomized experimental design using computer-assisted telephone interviewing system was conducted to collect data from representative samples aged 18 years or older. Individual characteristics among the six groups indicated that all of the sub-samples were similar in terms of gender, age, education, marital status, working status, and monthly income. Results of the graded response model suggested that a scale with at least three response categories on the positive side and no more than two on the negative side will be suitable for the single measure of general happiness. Findings of ordered logit regression on happiness were also in favor of an unbalanced response design. A discussion of the result is provided. [ABSTRACT FROM AUTHOR]

Background: We examined the ability of a procalcitonin (PCT) protocol to detect vesicoureteral reflux (VUR) and renal scarring (RS), evaluated procedural costs and radiation burden, and compared four representative guidelines for children with their first febrile urinary tract infection (UTI). Methods: Children aged ≤2 years with their first febrile UTI who underwent renal ultrasonography (US), acute and late technetium-99m (Tc)-dimercaptosuccinic acid scan, and voiding cystourethrography were prospectively studied. The representative guidelines applied in a retrospective simulation included the American Academy of Pediatrics (AAP), National Institute of Clinical Excellence, top-down approach (TDA), and Italian Society of Pediatric Nephrology (ISPN). These were compared in terms of ability to detect abnormalities, procedural costs and radiation. Results: Of 278 children analyzed, 172 (61.9 %) had acute pyelonephritis. There was VUR in 101 (36.3 %) children, including 73 (26.3 %) with grades III-V VUR. RS was identified in 75 (27.0 %) children. To detect VUR, TDA and PCT had the highest sensitivity for grades I-V VUR (80.2 %) and III-V VUR (94.5 %), respectively, whereas AAP had the highest specificity for I-V VUR (77.4 %) and III-V VUR (78.0 %), respectively. TDA and PCT had the highest sensitivity (100 %) for detecting RS. The highest cost and radiation dose was associated with TDA, whereas AAP had the least expenditure and radiation exposure. By multivariate analysis, PCT and VUR, especially grades III-V, were independent predictors of RS. Conclusions: There is no perfect guideline for first febrile UTI children. The PCT protocol has good ability for detecting high-grade VUR and RS. If based on available imaging modalities and reducing cost and radiation burden, clinical suggestions in the AAP guidelines represent a considerable protocol. [ABSTRACT FROM AUTHOR]

We consider a dual distribution channel in which a vertically integrated manufacturer competes with a downstream rival in a retail market and also sells an input to the rival. We use a signalling model with a continuum of types to examine a situation in which the manufacturer has private information on the production cost of its retail product. We show that in a separating equilibrium under Cournot ( Bertrand) retail competition, the manufacturer signals the uncompetitiveness (competitiveness) of its firm by charging a smaller input price than the optimal price under complete information. [ABSTRACT FROM AUTHOR]

The Tamsui River basin is located in Northern Taiwan and encompasses the most metropolitan city in Taiwan, Taipei City. The Taiwan Environmental Protection Administration (EPA) has established 38 water quality monitoring stations in the Tamsui River basin and performed regular river water quality monitoring for the past two decades. Because of the limited budget of the Taiwan EPA, adjusting the monitoring program while maintaining water quality data is critical. Multivariate analysis methods, such as cluster analysis (CA), factor analysis (FA), and discriminate analysis (DA), are useful tools for the statistically spatial assessment of surface water quality. This study integrated CA, FA, and DA to evaluate the spatial variance of water quality in the metropolitan city of Taipei. Performing CA involved categorizing monitoring stations into three groups: high-, moderate-, and low-pollution areas. In addition, this categorization of monitoring stations was in agreement with that of the assessment that involved using the simple river pollution index. Four latent factors that predominantly influence the river water quality of the Tamsui River basin are assessed using FA: anthropogenic pollution, the nitrification process, seawater intrusion, and geological and weathering processes. We plotted a spatial pattern using the four latent factor scores and identified ten redundant monitoring stations near each upstream station with the same score pattern. We extracted five significant parameters by using DA: total organic carbon, total phosphorus, As, Cu, and nitrate, with spatial variance to differentiate them from the polluted condition of the group obtained by using CA. Finally, this study suggests that the Taiwan EPA can adjust the surface water-monitoring program of the Tamsui River by reducing the monitoring stations to 28 and the measured chemical parameters to five to lower monitoring costs. [ABSTRACT FROM AUTHOR]

Topographic changes during the Pleistocene glacial/interglacial cycles affected the distribution of coastline mangroves and influenced their population genetic structure. The submergence of the continental shelf off southeast China during the postglacial age caused coastline expansions and resulted in the colonization of mangroves. Here, we performed multilocus genome scans using amplified fragment-length polymorphisms to explore the effects of topography and natural selection in structuring Kandelia obovata populations. Long-term isolation by the Taiwan Strait since the end of the last glacial maximum, which obstructed gene flow, differentiated the Taiwanese and Chinese populations. Founders that colonized from both outlets of the Taiwan Strait were sourced from the northern South China Sea and the Ryukyus, thereby creating a melting pot in the Taiwan Strait. Inner-strait currents played roles as vectors for propagule dispersal among populations. Upon examination of the allele-frequency distributions of outlier loci, most negative outliers reflected the widespread polymorphisms shared by common ancestors. Furthermore, significant differentiation in the genetic components of positive outliers between this and other populations and the negative correlation with geographic distance suggested the presence of geography- or latitude-independent population divergence. Restored populations were compared with their sources and revealed biased sampling of nursery seedlings, which caused within-population substructures and reduced effective population sizes. This study indicated that multiple factors affect the population structure of the mangroves off southeast China. [ABSTRACT FROM AUTHOR]

Objective: Reactive oxygen species (ROS) plays a critical role in the regulation of NLRP3 inflammasome activation. However, the ROS-mediated signaling pathways controlling NLRP3 inflammasome activation are not well defined. Methods: Using lipopolysaccharide (LPS) and adenosine triphosphate (ATP) activated murine macrophages as the testing model, cytokine release and protein expression were quantified by enzyme-linked immunosorbent assay and Western blot, respectively. ROS was scavenged by N-acetyl cysteine; NADPH oxidase, the major source of ROS, was inhibited by diphenyliodonium, apocynin or gp91-phox siRNA transfection; and protein kinase was inhibited by its specific inhibitor. Results: LPS-induced NLRP3 protein expression was regulated through the NADPH oxidase/ROS/NF-κB-dependent, JAK2/PI3-kinase/AKT/NF-κB-dependent, and MAPK-dependent pathways, while ATP-induced caspase-1 activation was regulated through the NADPH oxidase/ROS-dependent pathway. Conclusions: These results demonstrate that ROS regulates not only the priming stage, but also the activation stage, of NLRP3 inflammasome activation in LPS + ATP-activated macrophages. [ABSTRACT FROM AUTHOR]

In mammals, androgens appear to enhance the development of primary ovarian follicles, but PI3K (phosphoinositide 3-kinases) pathway is well recognized as one of the critical pathways in early follicular development. Roles of the PI3K were revealed by deletion of PTEN (phosphatase and tensin homolog on chromosome 10). PTEN is demonstrated to play an important role in the early stage of follicle development. In the Japanese eel, two forms of PTEN have been cloned, but what their functions on the development of early ovarian follicles are still not clear. The natural blockage and inducible of ovarian development was a benefit to address this question in the eel. Testosterone (T) shows to ameliorate the early ovarian development in the eel. The aims of this study were to elucidate the two forms of PTEN by cellular and physiological criteria and to study the effects of T on the ovarian PTEN production in the exogenous pituitary extracts-stimulated eel. Our results suggested that two forms of PTEN are existing in the Japanese eel, and eel ovarian development corresponded to the decrease in ovarian PTEN expression, vice versa. In addition, the supplement of T on eel early ovarian development can be attributed to its PTEN inhibitor role. [ABSTRACT FROM AUTHOR]

To further understand natural variation and local adaptation in the evolution of plant defense, we analyzed polymorphism data of nucleotide-binding site (NBS) sequences of Rhododendron at both the species and population levels. Multiple duplication events were found in NBS sequence evolution in Rhododendron genomes, which resulted in six clades: A-F. Our results of several NBS clade pair comparisons showed significant evolutionary rate changes based on differences in substitution rates between NBS-encoding protein clades (type I functional divergence). Pairwise comparisons of NBS clades further revealed that many amino acids displayed radical biochemical property changes causing a shift in amino acid preferences between NBS-encoding protein clades (type II functional divergence). Such divergent evolution of NBSs is likely a consequence of positive selection related to differentiation of recognition signals in response to different pathogens. Primers specific to clades B and C, which differed in the number of radical amino acid changes causing type II functional divergence and levels of nucleotide diversities, were further used to amplify population clades B and C NBS sequences of Rhododendron formosanum populations. Higher levels of net nucleotide divergences (measured by D) between R. formosanum populations were found based on NBS sequences of population clade B compared to population clade C, suggesting local adaptation of population clade B NBS sequences. Local adaptation can be further inferred for R. formosanum population clade B NBS sequences because of significant Φ based on variation in nonsynonymous substitutions. Furthermore, local adaptation was also suggested by no significant correlation of population pairwise F between population clades B and C in R. formosanum. [ABSTRACT FROM AUTHOR]

Recently there are many organizations conducting projects on ranking world universities from different perspectives. These ranking activities have made impacts and caused controversy. This study does not favor using bibliometric indicators to evaluate universities' performances, but not against the idea either. We regard these ranking activities as important phenomena and aim to investigate correlation of different ranking systems taking bibliometric approach. Four research questions are discussed: (1) the inter-correlation among different ranking systems; (2) the intra-correlation within ranking systems; (3) the correlation of indicators across ranking systems; and (4) the impact of different citation indexes on rankings. The preliminary results show that 55 % of top 200 universities are covered in all ranking systems. The rankings of ARWU and PRSPWU show stronger correlation. With inclusion of another ranking, WRWU (2009-2010), these rankings tend to converge. In addition, intra-correlation is significant and this means that it is possible to find out some ranking indicators with high degree of discriminativeness or representativeness. Finally, it is found that there is no significant impact of using different citation indexes on the ranking results for top 200 universities. [ABSTRACT FROM AUTHOR]

Diabetes is often associated with atherothrombosis. It is unknown whether high glucose can modulate the expression of tissue factor (TF) and thrombomodulin (TM) in human aortic endothelial cells (HAECs). HAECs were treated with a lower-degree high glucose condition (LG, 11.2 mM) for 8 days and a higher-degree high glucose condition (HG, 30 mM) for 4-6 h. Methoxyphenyl tetrazolium inner salt assay, real-time polymerase chain reaction, western blot, and TF activity assay were performed. In HAECs, both LG and HG conditions were nontoxic. LG caused a 74 ± 20% decrease ( P = 0.273) and HG caused a 57 ± 5% decrease in TF mRNA expression ( P = 0.001). LG caused a 53 ± 13% decrease ( P = 0.036) and HG caused a 75 ± 10% decrease in TF protein expression ( P = 0.096). TF activity was not significantly changed by LG (127 ± 13%, P = 0.40) or HG treatments (120 ± 42%, P = 0.70). In contrast, LG caused a 153 ± 16% increase ( P = 0.03) and HG caused a 211 ± 20% increase in TM mRNA expression ( P = 0.005). LG caused a 131 ± 31% increase ( P = 0.35) and HG caused a 140 ± 9% increase in TM protein expression ( P = 0.006). Different high glucose conditions do not provide the sufficient stress required to induce TF expression in HAECs. In contrast, high glucose conditions can induce TM expression in HAECs. [ABSTRACT FROM AUTHOR]

This article reports a perfusion 3D cell culture biochip with an incorporated electrical impedance measurement-based scheme for the quantification of cell number in the 3D cell culture construct. The biochip consists of culture chamber and fluidic channel for perfusion 3D cell culture followed by assembling the electrodes for impedance measurement. In this study, breast cancer cell line culture was performed using the proposed biochip. The perfusion 3D cell culture model was compared with a conventional static cell culture model in terms of environmental pH and metabolic activities of cells. Results revealed that the real value of the proposed biochip has its ability to maintain a stable and thus quantifiable culture condition. In addition, the metabolic activities of cells in the two culture models compared showed statistically different. It is concluded that the choice of a cell culture format can influence assay results. Moreover, after performing the 3D cell culture, electrical impedance measurement was conducted for the quantification of cell number in the 3D cell culture construct. The results indicated that the change in impedance was directly proportional to the cell number from 10 to 10 cells ml in the culture construct. As a whole, the proposed biochip not only can provide a stable, well-defined, and biologically meaningful culture environment, but also integrates the work of cell number quantification in a simple, economy, and time efficient manner. All of these traits are particularly useful for high-precision and high-throughput 3D cell culture-based assays. [ABSTRACT FROM AUTHOR]

The study compares the prediction performances of evapotranspiration by the FAO56 Penman-Monteith method and the pan evaporation method using the artificial neural network. A backpropagation neural network was adopted to determine the relationship between meteorological factors and evapotranspiration or evaporation. The evapotranspiration in the ChiaNan irrigated area of Tainan was considered. Weather data compiled by Irrigation Experiment Station of ChiaNan Irrigation Association were the input layer variables, including (1) the highest temperature, (2) the lowest temperature, (3) the average temperature, (4) the relative humidity, (5) the wind speed, (6) hours of sunlight, (7) amount of solar radiation, (8) the dew point, (9) morning ground temperature and (10) afternoon ground temperature. The importance of the ten weather factors was ranked by the general influence (GI) factor. Results show that the correlation coefficient between the evapotranspiration in 2004 calculated by FAO56 Penman-Monteith method and the one predicted by the neural network model with a hidden layer of ten nodes is 0.993. The actual evapotranspiration is 911.6 cm, and value prediction by the neural network is 896.4 cm, between which two values the error is 1.67%. The results reveal that the backpropagation neural network based on the FAO56 Penman-Monteith method can accurately predict evapotranspiration. However, the correlation coefficient between the actual evaporation in 2004 and the value prediction by the neural network with a hidden layer of ten nodes and an output layer with the pan evaporation as its target output is 0.708. The pan evaporation is 1,673.1 cm, while the value predicted by the backpropagation neural network is 1,451.7 cm, between which values the error is 13.23%. The backpropagation neural networks with pan evaporation as target outputs predict the evaporation with large errors. Moreover, the use of four agricultural weather factors (determined by the GI) including wind speed, average temperature, dew point and maximum temperature as input variables, and a hidden layer of three nodes in the backpropagation neural network model can successfully predict evapotranspiration based on the FAO56 Penman-Monteith method ( R = 0.98, error = 1.35%). [ABSTRACT FROM AUTHOR]

Nucleotide-binding site-leucine-rich repeats (NBS-LRR) gene families are one of the major plant resistance genes. Genomic NBS evolution was studied in many plant species for diverse arrays of NBS gene families. In this study, we focused on one family of NBS sequences in an attempt to understand how closely related NBS sequences evolved in the light of selection in domesticated plant species. A phylogenetic analysis revealed five major clades (A-E) and five subclades (A1-A5) within clade A of cloned NBS sequences. Positive selection was only detected in newly evolved NBS lineages in subclades of clade A. Positively selected codon sites were found among NBS sequences of clade A. A sliding-window analysis revealed that regions with Ka/ Ks ratios of >1 were in the inter-motifs when paired clades were compared, but regions with Ka/ Ks ratios of >1 were found across NBS sequences when subclades of clade A were compared. Our results based on a family of closely related NBS sequences showed that positive selection was first exerted on specific lineages across all NBS sequences after selective constraints. Subsequently, sequences with mutations in commonly conserved motifs were scrutinized by purifying selection. In the long term, conserved high frequency alleles in commonly conserved motifs and changes in inter-motifs were maintained in the investigated family of NBS sequences. Moreover, codons identified to be under positive selection in the inter-motifs were mainly located in regions involved in functions of ATP binding or hydrolysis. [ABSTRACT FROM AUTHOR]

This study explores the consistency between objective indicators and subjective perceptions of quality of life in a ranking of survey data for cities and counties in Taiwan. Data used for analysis included the Statistical Yearbook of Hsiens and Municipalities and the Survey on Living Conditions of Citizens in Taiwan, both given for the year 2000. The Quality of life was examined in seven domains: medical services, domestic finances, work, education, leisure, public safety, and environmental quality. Subjective and objective rankings for each domain of quality of life for 23 areas (some areas are cities and some are counties) are compared. Analysis by means of nonparametric correlation coefficients indicates that there is no significant correlation between objective indicators and subjective perceptions, except in Education and Environmental Quality. Objective indicators of Environmental Quality (air pollution and garbage) are positively correlated with subjective satisfaction with residential environment. But inexplicably, higher levels of literacy and educational achievement are negatively correlated with satisfaction with the educational system. It may be considered that disparity in either average objective conditions or in average subjective perceptions may not adequately depict quality of life differences. [ABSTRACT FROM AUTHOR]

An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]

18F-fluorodeoxyglucose positron emission tomography (FDG-PET) [or PET/computed tomography (CT)] is more likely to show false-negative results when it is performed shortly after chemotherapy and/or radiotherapy because of “metabolic stunning”. The present study aimed to evaluate the influence of I-131 therapy on FDG uptake and the detection of recurrence or metastasis of differentiated thyroid cancer (DTC). We retrospectively enrolled 16 consecutive FDG-PET/CT studies which had been performed in patients with DTC with elevated thyroglobulin (TG) but negative I-131 whole-body scan. All studies were performed under l-thyroxine suppression. The patients were divided into groups A and B for PET/CT performed within 4 months of I-131 therapy or no such therapy, respectively. Each lesion identified on PET/CT was characterized using a 5-point scale by visual analysis: 0 = definitely benign, 1 = probably benign, 2 = equivocal, 3 = probably malignant, and 4 = definitely malignant. The maximum standardized uptake value (SUVmax) in each lesion was also measured for semiquantitative analysis. We compared the visual grading and SUVmax of the lesion of highest FDG uptake between groups A and B. For visual analysis, group B had significantly more patients with an uptake score of 3 or 4 than group A (80% vs. 17%, P = 0.01). In addition, there were significantly more equivocal results from group A than from group B (67% vs. 10%, P = 0.02). If the patients with the highest uptake scores of 2, 3, and 4 were considered to be positive for local recurrence or metastasis, there would be no significant difference between the positive rates of groups A and B (83% vs. 90%, P = 0.7). However, the mean SUVmax of positive results was significantly lower for group A than for group B (3.1 ± 0.9 and 6.6 ± 3.5, respectively, P = 0.02). The preliminary results suggested that FDG uptake in DTC may be negatively influenced by I-131 therapy within 4 months, resulting in lower FDG uptake and more equivocal results. Further studies are necessary to determine whether it is secondary to “metabolic stunning” caused by I-131 therapy. [ABSTRACT FROM AUTHOR]