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Perception of biotic and abiotic stresses often leads to stomatal closure in plants1,2. Rapid influx of calcium ions (Ca2+) across the plasma membrane has an important role in this response, but the identity of the Ca2+ channels involved has remained elusive3,4. Here we report that the Arabidopsis thaliana Ca2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca2+ influx mechanisms in response to different stresses. A study in Arabidopsis thaliana shows that the immune receptor-associated cytosolic kinase BIK1 phosphorylates OSCA1.3 and identifies OSCA1.3 as the pathogen-responsive Ca2+-permeable channel that regulates stomatal closure. [ABSTRACT FROM AUTHOR]

Initialization of self-organizing maps is typically based on random vectors within the given input space. The implicit problem with random initialization is the overlap (entanglement) of connections between neurons. In this paper, we present a new method of initialization based on a set of self-similar curves known as Hilbert curves. Hilbert curves can be scaled in network size for the number of neurons based on a simple recursive (fractal) technique, implicit in the properties of Hilbert curves. We have shown that when using Hilbert curve vector (HCV) initialization in both classical SOM algorithm and in a parallel-growing algorithm (ParaSOM), the neural network reaches better coverage and faster organization. [ABSTRACT FROM AUTHOR]

New sequencing methods generate data that can allow the assembly of microbial genome sequences in days. With such revolutionary advances in technology come new challenges in methodologies and informatics. In this article, we review the capabilities of high-throughput sequencing technologies and discuss the many options for getting useful information from the data. [ABSTRACT FROM AUTHOR]

An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]

The article discusses the importance of flexible, scalable approaches to bioinformatics provision in smaller research institutes and university research departments. Norwich, England-based Sainsbury Laboratory has transitioned to a bottom-up model of bioinformatics provision in order to adapt to the influx of big data sets from high-throughput approaches. Tools, such as the workflow engineering environment Galaxy, are used for reducing the perceived barrier in data management and access.