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A severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron subvariant, BA.2.86, has emerged and spread to numerous countries worldwide, raising alarm because its spike protein contains 34 additional mutations compared with its BA.2 predecessor1. We examined its antigenicity using human sera and monoclonal antibodies (mAbs). Reassuringly, BA.2.86 was no more resistant to human sera than the currently dominant XBB.1.5 and EG.5.1, indicating that the new subvariant would not have a growth advantage in this regard. Importantly, sera from people who had XBB breakthrough infection exhibited robust neutralizing activity against all viruses tested, suggesting that upcoming XBB.1.5 monovalent vaccines could confer added protection. Although BA.2.86 showed greater resistance to mAbs to subdomain 1 (SD1) and receptor-binding domain (RBD) class 2 and 3 epitopes, it was more sensitive to mAbs to class 1 and 4/1 epitopes in the ‘inner face’ of the RBD that is exposed only when this domain is in the ‘up’ position. We also identified six new spike mutations that mediate antibody resistance, including E554K that threatens SD1 mAbs in clinical development. The BA.2.86 spike also had a remarkably high receptor affinity. The ultimate trajectory of this new SARS-CoV-2 variant will soon be revealed by continuing surveillance, but its worldwide spread is worrisome.A severe acute respiratory syndrome coronavirus 2 Omicron subvariant, BA.2.86, was found to be no more resistant to human sera than the currently dominant XBB.1.5 and EG.5.1, but it had a remarkably higher receptor affinity. [ABSTRACT FROM AUTHOR]

Wildfires are one of the most frequent natural disasters which significantly harm the environment, society, and the economy. Transfer learning algorithms and modern machine learning tools can help in early forest fire prediction, detection, and dynamic fire quenching. A group of drones that has high-definition image processing and decision-making capabilities are used to detect the forest fires in the very early stage. The proposed system generates a fire quenching plan using particle swarm optimization technique and alerts the fire and rescue department for a quick action, thereby stop the forest fire at an early stage. Also, the drone network plays a major role to track the live status of forest fire and quenches the fire. ResNet, VGGNet, MobileNet, AlexNet, and GoogLeNet are used to detect the forest fire hazards. The experimental results prove that the proposed technique GoogLeNet-TL provides 96% accuracy and 97% F1 score in comparison with the state-of-the-art deep learning models. [ABSTRACT FROM AUTHOR]

Prophylactic vaccines for SARS-CoV-2 have lowered the incidence of severe COVID-19, but emergence of viral variants that are antigenically distinct from the vaccine strains are of concern and additional, broadly acting preventive approaches are desirable. Here, we report on a glycolipid termed 7DW8-5 that exploits the host innate immune system to enable rapid control of viral infections in vivo. This glycolipid binds to CD1d on antigen-presenting cells and thereby stimulates NKT cells to release a cascade of cytokines and chemokines. The intranasal administration of 7DW8-5 prior to virus exposure significantly blocked infection by three different authentic variants of SARS-CoV-2, as well as by respiratory syncytial virus and influenza virus, in mice or hamsters. We also found that this protective antiviral effect is both host-directed and mechanism-specific, requiring both the CD1d molecule and interferon- γ . A chemical compound like 7DW8-5 that is easy to administer and cheap to manufacture may be useful not only in slowing the spread of COVID-19 but also in responding to future pandemics long before vaccines or drugs are developed. 7DW8-5 is a glycolipid that binds CD1d and stimulates invariant natural killer T (iNKT) cells. Here the authors show that 7DW8-5, when administered intranasally, provides prophylactic anti-viral effects against influenza, RSV, and SARS-CoV-2 in mice or hamsters, and that this effect is mediated by iNKT cells and IFN-γ. [ABSTRACT FROM AUTHOR]

Atriplex lentiformis is a halophytic plant species used for desalination and phytoremediation. The plant tolerates abiotic constraints, such as salinity, drought, and toxic metals. It is also used as a fodder for domestic animals. It grows luxuriantly at 100–400-mM NaCl concentrations without any toxic symptoms. In the present investigation effects of biological amendments—PGPB (Plant growth-promoting bacteria—Bradyrhizobium japonicum—NCIM5350 and Pseudomonas fluorescens—NCIM2100), organic manure (OM), and chemical amendment—Ethylene Diamine Tetra Acetic acid (EDTA) on Atriplex lentiformis were explored in cadmium- and nickel-contaminated soil. Heavy metal resistance and plant growth-promoting traits of PGPB were also analyzed. Augmentation with a combination of both PGPB and OM, A. lentiformis displayed maximum uptake of Ni (45.67 mg kg−1 in roots; 24.68 mg kg−1 in shoots) and Cd (14.15 mg kg−1 in roots; 7.19 mg kg−1 in shoots). Highest Ni uptake in shoots was observed under the EDTA amendment (25.33 mg kg−1). Metal uptake by A. lentiformis under NCIM2100 was greater than NCIM5350 for both Cd and Ni (10.57 and 43.87 mg kg−1). Among all the amendments highest metal uptake was recorded under bio-organic treatments (PGPB1 + PGPB2 + OM) for both Cd and Ni (14.15 and 45.67 mg kg−1), respectively. The results showed that this association has significantly improved the plant height, biomass, chlorophyll, MDA (Malondialdehyde) content, and the activity of antioxidative enzymes (CAT, APX, and SOD) which exhibited a positive correlation with metal uptake at 1% level of significance and the potency of synergistic impact of microbial consortium, while EDTA reduced the growth of the plant. Metal uptake under EDTA was also much lower than biological amendments. Higher metal values in roots establishes A. lentiformis as a phytostabilizer thus indicating its suitability as a safer forage. Biological amendments-based phytoremediation holds great promise and could be used in future to give further impetus to the antioxidative defense, phytoremedial potential, and growth of this and other important forage plants. [ABSTRACT FROM AUTHOR]

The SARS-CoV-2 3CL protease is a critical drug target for small molecule COVID-19 therapy, given its likely druggability and essentiality in the viral maturation and replication cycle. Based on the conservation of 3CL protease substrate binding pockets across coronaviruses and using screening, we identified four structurally distinct lead compounds that inhibit SARS-CoV-2 3CL protease. After evaluation of their binding specificity, cellular antiviral potency, metabolic stability, and water solubility, we prioritized the GC376 scaffold as being optimal for optimization. We identified multiple drug-like compounds with <10 nM potency for inhibiting SARS-CoV-2 3CL and the ability to block SARS-CoV-2 replication in human cells, obtained co-crystal structures of the 3CL protease in complex with these compounds, and determined that they have pan-coronavirus activity. We selected one compound, termed coronastat, as an optimized lead and characterized it in pharmacokinetic and safety studies in vivo. Coronastat represents a new candidate for a small molecule protease inhibitor for the treatment of SARS-CoV-2 infection for eliminating pandemics involving coronaviruses. Small molecule drugs promise to remain a valuable tool in controlling the ongoing COVID-19 pandemic. Here the authors describe optimized drug-like small molecule inhibitors of the SARS-CoV-2 3CL protease for potential treatment of COVID-19. [ABSTRACT FROM AUTHOR]

SARS-CoV-2 Omicron subvariants BA.2.12.1 and BA.4/5 have surged notably to become dominant in the United States and South Africa, respectively1,2. These new subvariants carrying further mutations in their spike proteins raise concerns that they may further evade neutralizing antibodies, thereby further compromising the efficacy of COVID-19 vaccines and therapeutic monoclonals. We now report findings from a systematic antigenic analysis of these surging Omicron subvariants. BA.2.12.1 is only modestly (1.8-fold) more resistant to sera from vaccinated and boosted individuals than BA.2. However, BA.4/5 is substantially (4.2-fold) more resistant and thus more likely to lead to vaccine breakthrough infections. Mutation at spike residue L452 found in both BA.2.12.1 and BA.4/5 facilitates escape from some antibodies directed to the so-called class 2 and 3 regions of the receptor-binding domain3. The F486V mutation found in BA.4/5 facilitates escape from certain class 1 and 2 antibodies but compromises the spike affinity for the viral receptor. The R493Q reversion mutation, however, restores receptor affinity and consequently the fitness of BA.4/5. Among therapeutic antibodies authorized for clinical use, only bebtelovimab retains full potency against both BA.2.12.1 and BA.4/5. The Omicron lineage of SARS-CoV-2 continues to evolve, successively yielding subvariants that are not only more transmissible but also more evasive to antibodies.Findings from a systematic antigenic analysis of these surging Omicron subvariants that this lineage of SARS-CoV-2 continues to evolve, successively yielding subvariants that are not only more transmissible but also more evasive to antibodies. [ABSTRACT FROM AUTHOR]

Background: During coronavirus pandemic, an unpredictable pile of biomedical waste (BMW) gathers at the top. India produces 710 tonnes of biomedical waste daily. The contribution of COVID-19 related biomedical waste was 126 tonnes per day in first wave of the pandemic. BMW's rapid growth is putting a strain on current waste management facilities, especially in developing countries. A sudden boost in biomedical waste needs rapid and proper segregation and disposal methods to avoid future consequences. From literatures and statistical data available on Central Pollution Control Board (CPCB) it shows that India lags behind in large-scale sorting, collection, careful storage, transfer and disposal of bio waste. India has its own guidelines set by the CPCB to ensure the safe disposal of biomedical waste during diagnosis, treatment and quarantine of COVID-19 patients. Although there are strict guidelines for bio-waste management, many hospitals in the process of implementing them often dispose of waste in inappropriate, chaotic and indiscriminate ways due to negligence or laziness. Often, due to poor separation practices, hospital waste is mixed with general waste, resulting in harmful overall waste flow. Waste disposal handlers are not safe due to their exposure to various health risks and inadequate training in waste management. The present review sheds light on guidelines, measures, and challenges related to biomedical waste management. Short conclusion: Improper waste separation leads to improper waste disposal. Waste generation and management issues are causing daily problems as they have a profound impact on the dramatically changing global environment, including air, water and soil pollution. In addition, BMW's daily production and its processing are inversely proportional. This situation suggests that India will soon be drowning in its own garbage. The focus of this review is on the generation and disposal of biomedical waste. Based on a review of the literature, this evaluation provides a comparative picture of the current status of waste generation, national waste management strategies, and some measures to contribute to waste management and avoid future disasters. [ABSTRACT FROM AUTHOR]

Bioremediation itself is considered to be a cost effective soil clean-up technique and preferred over invasive physical and chemical treatments. Besides increasing efficiency, application of genetic engineering has led to reduction in the time duration required to achieve remediation, overcoming the so called 'Achilles heel' of Bioremediation. Omics technologies, namely genomics, transcriptomics, proteomics, and metabolomics, are being employed extensively to gain insights at genetic level. A wise synchronised application of these approaches can help scrutinize complex metabolic pathways, and molecular changes in response to heavy metal stress, and also its fate i.e., uptake, transport, sequestration and detoxification. In the present review, an account of some latest achievements made in the field is presented. [ABSTRACT FROM AUTHOR]

The B.1.1.529/Omicron variant of SARS-CoV-2 was only recently detected in southern Africa, but its subsequent spread has been extensive, both regionally and globally1. It is expected to become dominant in the coming weeks2, probably due to enhanced transmissibility. A striking feature of this variant is the large number of spike mutations3 that pose a threat to the efficacy of current COVID-19 vaccines and antibody therapies4. This concern is amplified by the findings of our study. Here we found that B.1.1.529 is markedly resistant to neutralization by serum not only from patients who recovered from COVID-19, but also from individuals who were vaccinated with one of the four widely used COVID-19 vaccines. Even serum from individuals who were vaccinated and received a booster dose of mRNA-based vaccines exhibited substantially diminished neutralizing activity against B.1.1.529. By evaluating a panel of monoclonal antibodies against all known epitope clusters on the spike protein, we noted that the activity of 17 out of the 19 antibodies tested were either abolished or impaired, including ones that are currently authorized or approved for use in patients. Moreover, we also identified four new spike mutations (S371L, N440K, G446S and Q493R) that confer greater antibody resistance on B.1.1.529. The Omicron variant presents a serious threat to many existing COVID-19 vaccines and therapies, compelling the development of new interventions that anticipate the evolutionary trajectory of SARS-CoV-2.The B.1.1.529/Omicron variant of SARS-CoV-2 is resistant to neutralization by serum not only from patients who recovered from COVID-19, but also from individuals vaccinated with one of the four widely used COVID-19 vaccines. [ABSTRACT FROM AUTHOR]

Since the start of the COVID-19 pandemic, SARS-CoV-2 has caused millions of deaths worldwide. Although a number of vaccines have been deployed, the continual evolution of the receptor-binding domain (RBD) of the virus has challenged their efficacy. In particular, the emerging variants B.1.1.7, B.1.351 and P.1 (first detected in the UK, South Africa and Brazil, respectively) have compromised the efficacy of sera from patients who have recovered from COVID-19 and immunotherapies that have received emergency use authorization1–3. One potential alternative to avert viral escape is the use of camelid VHHs (variable heavy chain domains of heavy chain antibody (also known as nanobodies)), which can recognize epitopes that are often inaccessible to conventional antibodies4. Here, we isolate anti-RBD nanobodies from llamas and from mice that we engineered to produce VHHs cloned from alpacas, dromedaries and Bactrian camels. We identified two groups of highly neutralizing nanobodies. Group 1 circumvents antigenic drift by recognizing an RBD region that is highly conserved in coronaviruses but rarely targeted by human antibodies. Group 2 is almost exclusively focused to the RBD–ACE2 interface and does not neutralize SARS-CoV-2 variants that carry E484K or N501Y substitutions. However, nanobodies in group 2 retain full neutralization activity against these variants when expressed as homotrimers, and—to our knowledge—rival the most potent antibodies against SARS-CoV-2 that have been produced to date. These findings suggest that multivalent nanobodies overcome SARS-CoV-2 mutations through two separate mechanisms: enhanced avidity for the ACE2-binding domain and recognition of conserved epitopes that are largely inaccessible to human antibodies. Therefore, although new SARS-CoV-2 mutants will continue to emerge, nanobodies represent promising tools to prevent COVID-19 mortality when vaccines are compromised.Multivalent nanobodies against SARS-CoV-2 from mice engineered to produce camelid nanobodies recognize conserved epitopes that are inaccessible to human antibodies and show promise as a strategy for dealing with viral escape mutations. [ABSTRACT FROM AUTHOR]

The COVID-19 pandemic has had widespread effects across the globe, and its causative agent, SARS-CoV-2, continues to spread. Effective interventions need to be developed to end this pandemic. Single and combination therapies with monoclonal antibodies have received emergency use authorization1–3, and more treatments are under development4–7. Furthermore, multiple vaccine constructs have shown promise8, including two that have an approximately 95% protective efficacy against COVID-199,10. However, these interventions were directed against the initial SARS-CoV-2 virus that emerged in 2019. The recent detection of SARS-CoV-2 variants B.1.1.7 in the UK11 and B.1.351 in South Africa12 is of concern because of their purported ease of transmission and extensive mutations in the spike protein. Here we show that B.1.1.7 is refractory to neutralization by most monoclonal antibodies against the N-terminal domain of the spike protein and is relatively resistant to a few monoclonal antibodies against the receptor-binding domain. It is not more resistant to plasma from individuals who have recovered from COVID-19 or sera from individuals who have been vaccinated against SARS-CoV-2. The B.1.351 variant is not only refractory to neutralization by most monoclonal antibodies against the N-terminal domain but also by multiple individual monoclonal antibodies against the receptor-binding motif of the receptor-binding domain, which is mostly due to a mutation causing an E484K substitution. Moreover, compared to wild-type SARS-CoV-2, B.1.351 is markedly more resistant to neutralization by convalescent plasma (9.4-fold) and sera from individuals who have been vaccinated (10.3–12.4-fold). B.1.351 and emergent variants13,14 with similar mutations in the spike protein present new challenges for monoclonal antibody therapies and threaten the protective efficacy of current vaccines.The SARS-CoV-2 variant B.1.1.7 can be neutralized by convalescent sera or sera from vaccinated individuals, whereas the B.1.351 variant is resistant to neutralization by these sera and by several monoclonal antibodies that are in clinical use. [ABSTRACT FROM AUTHOR]

Teaching and Learning confined to within the four walls of a classroom or even online Learning through Massive Online Courses (MOOCs) and other Learning Content Management Systems (LCMS) are no longer seen as the optimal approach for competency and skills development, especially for working professionals. Each of these busy learners have their own training needs and prior knowledge. Adopting the one-size-fits-all teaching approach is definitely not effective, motivating and encouraging. For some learners, the content might be too easy and for others, it might be too difficult. This is why this research presents the use of SMART Learning Environment that makes use of Intelligent Techniques to personalise the learning materials for each learner. This mismatch in skills is becoming a pressing issue and is having a direct impact on the ICT Sector, which is one of the pillars of the Mauritian Economy. This research, therefore, besides proposing a novel approach to learning, also attempts to address an issue of national importance. Data was collected during three phases, namely an Expert Reference Group Discussion, a pre-test questionnaire and a survey questionnaire. The Expert Reference Group Discussion was carried out to further understand the training needs and expectations of Cybersecurity professionals in Mauritius. A SMART Learning Environment making use of Artificial Neural Networks and Backpropagation Algorithm to personalise learning materials was eventually designed and implemented. The major findings of this research were that personalisation of learning materials through the use of a SMART Learning Environment can be used to address the training needs of Cybersecurity professionals in Mauritius. [ABSTRACT FROM AUTHOR]

We report the identification of three structurally diverse compounds – compound 4, GC376, and MAC-5576 – as inhibitors of the SARS-CoV-2 3CL protease. Structures of each of these compounds in complex with the protease revealed strategies for further development, as well as general principles for designing SARS-CoV-2 3CL protease inhibitors. These compounds may therefore serve as leads for the basis of building effective SARS-CoV-2 3CL protease inhibitors. The essential SARS-CoV-2 3CL protease is of interest as a drug target. Here, the authors identify three 3CL inhibitors and characterize them both in vitro and with a cell-based assay, and they also present the inhibitor-bound 3CL crystal structures, which may allow for the design of improved compounds. [ABSTRACT FROM AUTHOR]

Disposal of industrial waste is of great concern because it releases toxic metals resulting into the degradation of environments and consequently causes health effects on humans. The present study was carried out with the objectives of identifying the possible source and migration of contaminants in the surface water and groundwater and the associated health risks in the industrial region of Ranipet, Tamil Nadu, India. A total of 22 water samples were collected and analysed for the concentration of major ions and trace elements. Chemical speciation of chromium and lead in surface and groundwater was calculated using the geochemical code, PHREEQC. The concentration of total dissolved solids, chromium and lead was high in the surface water than groundwater in this region. The chromium in most of the sampled water is dominated with Cr(OH)2+ and Cr(OH)3 species and the dominant species of lead was PbCO3. The major source of contamination is the waste dumped in this region and also the improper disposal of effluents from the small-scale industries in this region. Use of groundwater with the present level of chromium and lead in this region will cause several health effects through oral and dermal pathways. Hence, stringent monitoring of quality of water sources of this region, enforcement of regulation of the disposal of wastes from the industries, recover and treat the dumped solid waste are very much necessary to prevent the spread of contamination. [ABSTRACT FROM AUTHOR]

Unlike regular wireless networks like Mobile Ad hoc NETworks (MANET) can be exploited instinctively devoid of necessitating any centralized administration or available network infrastructure. In such networks, the mobile nodes perform as relays to assist multi-hop communications and, in most cases, they are accountable for the entire networking functions. MANET is a very challenging task owing to the actuality that these systems may be subjected to attacks causing the network malicious. Among single- server and multi-server authentications, this paper intends to focus on multi-server authentication process in MANET or WSN or any heterogeneous network, which includes a secure protocol that provides a proper communication link between the nodes. In a single server network, both the credentials and authenticating procedure are handled by a single server. On the other hand, in a multi-server network, the credentials and authenticating procedure are handled by separate servers, and hence practically it is more robust against attacks. Hence, this paper develops a security protocol using Elliptic Curve Cryptography (ECC) in prime field. By adopting hash function, a secure way for key exchange is provided. Before transmitting the message from one node to another, it has to be ensured whether it is an authorized node or not. For authorizing purpose, a novel authenticating algorithm is developed based on two servers, a master server and normal server. Accordingly, the credentials and the processing steps are separated in such a way that compromising one of the servers never pose any threat to the whole authentication system. Initially, a node should register its credentials to the register centre, and those credentials are distributed to the server by a secure distribution algorithm where no hacking could be possible. The implemented technique is analyzed in terms of cost and security, and the results are obtained. [ABSTRACT FROM AUTHOR]

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic continues, with devasting consequences for human lives and the global economy1,2. The discovery and development of virus-neutralizing monoclonal antibodies could be one approach to treat or prevent infection by this coronavirus. Here we report the isolation of sixty-one SARS-CoV-2-neutralizing monoclonal antibodies from five patients infected with SARS-CoV-2 and admitted to hospital with severe coronavirus disease 2019 (COVID-19). Among these are nineteen antibodies that potently neutralized authentic SARS-CoV-2 in vitro, nine of which exhibited very high potency, with 50% virus-inhibitory concentrations of 0.7 to 9 ng ml−1. Epitope mapping showed that this collection of nineteen antibodies was about equally divided between those directed against the receptor-binding domain (RBD) and those directed against the N-terminal domain (NTD), indicating that both of these regions at the top of the viral spike are immunogenic. In addition, two other powerful neutralizing antibodies recognized quaternary epitopes that overlap with the domains at the top of the spike. Cryo-electron microscopy reconstructions of one antibody that targets the RBD, a second that targets the NTD, and a third that bridges two separate RBDs showed that the antibodies recognize the closed, 'all RBD-down' conformation of the spike. Several of these monoclonal antibodies are promising candidates for clinical development as potential therapeutic and/or prophylactic agents against SARS-CoV-2. A diverse collection of potent neutralizing antibodies against the SARS-CoV-2 spike protein have been isolated from five patients with severe COVID-19 and high serum neutralization titres. [ABSTRACT FROM AUTHOR]

Introduction: Elagolix is approved for the management of moderate-to-severe pain associated with endometriosis. The aim of this analysis was to develop a physiologically based pharmacokinetic (PBPK) model that describes the enzyme-transporter interplay involved in the disposition of elagolix and to predict the magnitude of drug-drug interaction (DDI) potential of elagolix as an inhibitor of P-glycoprotein (P-gp) and inducer of cytochrome P450 (CYP) 3A4.Methods: A PBPK model (SimCYP® version 15.0.86.0) was developed using elagolix data from in vitro, clinical PK and DDI studies. Data from DDI studies were used to quantify contributions of the uptake transporter organic anion transporting polypeptide (OATP) 1B1 and CYP3A4 in the disposition of elagolix, and to quantitatively assess the perpetrator potential of elagolix as a CYP3A4 inducer and P-gp inhibitor.Results: After accounting for the interplay between elagolix metabolism by CYP3A4 and uptake by OATP1B1, the model-predicted PK parameters of elagolix along with the DDI AUC∞ and Cmax ratios, were within 1.5-fold of the observed data. Based on model simulations, elagolix 200 mg administered twice daily is a moderate inducer of CYP3A4 (approximately 56% reduction in midazolam AUC∞). Simulations of elagolix 150 mg administered once daily with digoxin predicted an increase in digoxin Cmax and AUC∞ by 68% and 19%, respectively.Conclusions: A PBPK model of elagolix was developed, verified, and applied to characterize the disposition interplay between CYP3A4 and OATP1B1, and to predict the DDI potential of elagolix as a perpetrator under dosing conditions that were not tested clinically. PBPK model-based predictions were used to support labeling language for DDI recommendations of elagolix. [ABSTRACT FROM AUTHOR]

A study was conducted to determine the effect of ethylenediaminetetraacetic acid (EDTA) on phytoextraction potential of radish and cabbage. Plant biomass, photosynthetic pigments, proline and phenolics were significantly affected by the accumulation of heavy metals (HM). The metal uptake potential was increased significantly by the application of EDTA. Target hazard quotient (THQ) associated with exposure of these contaminants to food chain was calculated. Agronomic interventions to increase mineral levels in crops often increases the leaf concentrations only, the mineral concentration in edible portions are not increased at desired level due to low mobility of Zn in phloem. Since the leaves of both these crops are edible and a component of staple vegetarian diet, biofortification through Zn present in soil and its solubilization and mobilization through chelators can be implemented. However in no such instance these crops should be consumed when grown on Pb contaminated soil due to associated hazards. [ABSTRACT FROM AUTHOR]

Abstract: Monitoring of groundwater quality is essential in rural regions of developing nations, as most of the population depends on groundwater for various purposes. This study was carried out with the objective of assessing the groundwater quality to evaluate the suitability of water for drinking and irrigational purposes in northern Karnataka, India. Groundwater samples were collected from 68 wells once in three months from September 2013 to March 2014 and analysed for electrical conductivity, pH and major ions. Mixed Ca-Mg-Cl and Ca-HCO3 type of groundwater is dominant. The geochemical characteristics of the groundwater are believed to be the weird geologic and hydrogeologic condition that prevails in this region. The changes in geochemistry are due to the degree of weathering, rock-water interaction and evaporation processes. Groundwater is suitable for domestic and irrigation purposes except in Hattigudur and Doranhalli villages. This study is useful for assessing the environmental impact on groundwater due to industries and mining activities in future. [ABSTRACT FROM AUTHOR]

Abstract: Monitoring of groundwater quality is essential in rural regions of developing nations, as most of the population depends on groundwater for various purposes. This study was carried out with the objective of assessing the groundwater quality to evaluate the suitability of water for drinking and irrigational purposes in northern Karnataka, India. Groundwater samples were collected from 68 wells once in three months from September 2013 to March 2014 and analysed for electrical conductivity, pH and major ions. Mixed Ca-Mg-Cl and Ca-HCO3 type of groundwater is dominant. The geochemical characteristics of the groundwater are believed to be the weird geologic and hydrogeologic condition that prevails in this region. The changes in geochemistry are due to the degree of weathering, rock-water interaction and evaporation processes. Groundwater is suitable for domestic and irrigation purposes except in Hattigudur and Doranhalli villages. This study is useful for assessing the environmental impact on groundwater due to industries and mining activities in future. [ABSTRACT FROM AUTHOR]

Due to the important role of biochar (BC) in reducing metal-toxicity in plants, this study was aimed at assessing the potential of citrus epicarp-derived BC in ameliorating Cd toxicity in young Abelmoschus esculentus (okra) under low Cd toxicity. Okra was grown in soil amended with BC at four treatment levels for 49 days as follows: control (A), sole 1.4 mg Cd/kg-spiked soil (B), 1.4 mg Cd/kg-spiked soil + 1% BC (C) and 1.4 mg Cd/kg-spiked soil + 3% BC (D). The results showed a dose-dependent reduction in shoot accumulation of Cd due to the BC application. In addition, compared to control and sole Cd-amended soil, BC treatments (both at 1% and 3% w/w) decreased the oxidative stress, and enhanced activities of enzymatic and non-enzymatic antioxidants in the young okra. Generally, the application of BC to the soil was effective in ameliorating the Cd-induced oxidative stress in okra with limited shoot bioaccumulation of Cd. [ABSTRACT FROM AUTHOR]

Assessment of groundwater quality is an important aspect of water security, which is the key to ensure sustainable development. The objective of the study is to bring out an integrated approach for assessment of groundwater quality for drinking and irrigation purposes. Gogi region, Karnataka, India was chosen as the study area due to the effect of the presence of medium-grade uranium deposits. An integrated approach including the concentration of major ions, trace elements and uranium was employed to investigate the quality of groundwater. Totally, 367 groundwater samples were collected periodically from 52 wells distributes over the Gogi region and the parameters such as pH, electrical conductivity, total dissolved solids (TDS), Ca, Mg, Na, K, Cl, SO , NO , Zn, Pb, Cu, and uranium of groundwater were analysed. Spatial distribution maps of various chemical constituents were prepared using geographic information system and its temporal variation was plotted in box and whisker plot. The analytical data were compared with Bureau of Indian Standards and World Health Organisation standards to determine drinking water quality and parameters such as salinity hazard, alkalinity hazard and percent sodium were estimated to assess the irrigation quality. Multivariate statistical analysis by cluster analysis was also performed which results in two groups consisting of wells with unsuitable water for drinking purposes. Groundwater in about 15% of the sampling wells were found to be unsuitable for domestic purpose based on TDS and about 17% were unsuitable based on uranium concentration. Finally, integration of spatial variation in TDS and uranium reveals that about 25% of the wells were unsuitable for domestic purposes. It is suggested that such an integrated approach needs to be formulated considering major ions, trace elements and radioactive elements for proper assessment of water quality. Implementation of managed aquifer recharge structures in the study area is suggested since it would potentially reduce the concentration of ions. [ABSTRACT FROM AUTHOR]

For the effective use of available frequency bandwidth, cognitive radio technology is evolving in the last few years. In this, spectrum is shared between primary and secondary users. Secondary users also called as unlicensed users, makes use of part of spectrum which is not used by primary or licensed users. Hence secondary user systems must be capable of sensing the spectrum and should be able to reconfigure to transmit at different frequencies. For this it must consists of spectrum sensing circuit and a reconfigurable transceiver consisting of reconfigurable antenna. This paper discuss the single module of reconfigurable transceiver which dynamically allocates two different frequencies 2.4 and 3.1 GHz for transmission. Ultra-wideband patch antenna, spectrum sensing algorithm and reconfigurable patch antenna are also discussed. The module can be modified to transmit at more frequencies in the range 2.4-9 GHz by having reconfigurable devices such as LNAs, power amplifiers, local oscillators, mixers, etc. [ABSTRACT FROM AUTHOR]

This study was aimed to determine the uptake and accumulation potential of a weed (Abutilon indicum L.) for phytoremediation of soil contaminated with cadmium. Plants were grown in soil spiked with 0, 2.5, 5, 10, 15, 20, 25 mg/kg Cd, individually. Plants sample (root and shoot) were analyzed for Cd content at 30, 60, and 90 days and accumulation trends were characterized. A steady increase in Cd accumulation with increasing metal concentration and exposure period was observed for all treatments. Accumulation of Cd in roots was found to be 4.3–7.7 times higher than that of shoots. Statistically significant difference (P≤0.001) in mean metal content in root and shoot at successive days of study was recorded. Effect of Cd on growth and physiology was also evaluated. At higher Cd levels, root and shoot length and biomass of test plant were reduced significantly. Although, growth was delayed initially, it was comparable to control at the end of the study. Chlorophyll and proline content declined with the increase in Cd concentration at 30 and 60 days after treatment. However, at 90 days, values were more or less comparable to the control values showing the adaptability of test plant in Cd contamination. Considering the accumulation ability, BCF >1 (bioconcentration factor) and TF <1 (translocation factor) established A. indicum as a potential candidate plant for phytoremediation. Hence, phytoremediation employing indigenous weed species like A. indicum can be an ecologically viable option for sustainable and cost-effective management of heavy metal-contaminated soils. [ABSTRACT FROM AUTHOR]

Sustainable business strategies or technologies determine the market dynamism so as to withstand competitiveness. The evaluation of such strategies is a multi-criteria decision making (MCDM) problem. To deal with such problems, analytical hierarchy process (AHP) and analytical network process (ANP) could be deployed. AHP considers the distribution of goal amongst the elements being compared and judges the element which has the greater influence on the goal. In order to overcome the drawbacks with AHP, ANP could be used which is a holistic approach in which all attributes and alternatives involved are connected in a network system that accepts dependencies. Priorities which are established in ANP are the same way as they are in the AHP using pair wise comparisons and judgments. ANP is the most comprehensive framework for the analysis of societal, governmental and corporate decisions that are available today to the decision-maker. It is a process that allows one to include all the factors and criteria, tangible and intangible that has an effect on making a best decision. The paper reports a research study that is focused on the selection of the best alternative from the perspective of sustainability for an Indian relays manufacturing organization. [ABSTRACT FROM AUTHOR]

Jatropha curcas, a non-domesticated energy plant, has emerged as a source of biodiesel as it does not compete with the edible oil supplies. Realizing its importance, in vitro regeneration methods have been established to meet the demand of large scale supply of superior clones. However, no precise histological analysis has been conducted to document the in vitro morphogenesis in J. curcas. Here, we present a detailed histological description of the initiation of growth and morphogenesis from callus induced from immature cotyledon and embryonal axis of J. curcas. Microscopic observations revealed that the cells of sub-epidermis in immature cotyledon became meristematic and divided extensively to result in the formation of meristemoids within 3-4 weeks of culture. The meristemoid cells are compact, small, and exhibited densely stained cytoplasm compared to the loosely packed large non-meristematic cells. Presence of meristemoids in different developmental stages above the cambial zone, and the absence of vascular connection to the cotyledon explant led us to conclude that the origin of the meristemoids was adventitious in nature and regeneration is through organogenesis of morphogenic callus. On the other hand, callus derived from immature embryonal axis followed two types of regeneration-one type of shoot regeneration was via organogenesis while the second type was through multiplication of the pre-existing meristems. It was noticed that under similar experimental conditions, the process of organogenesis varied from explant to explant. The present study contributes to adequate knowledge and understanding the process of in vitro shoot morphogenesis in J. curcas. [ABSTRACT FROM AUTHOR]

Toxicity of lead in soil is well documented and established. Phytoremediation has gained attention as a cheap, easily applicable, and eco-friendly clean-up technology. Chemical methods are used to assess exact levels and type of pollutants but heavy metal content in soil can also be evaluated indirectly by estimation of phytotoxicity levels using bioassays. Plant bioassays through fast germinating cereals can indicate not only the level of pollution and its effects on growth and survival but also the progress of phytoremediation process. The performance of barley Hordeum vulgare L. seedlings as bioassay for assessment of changes in the levels of lead (Pb) at three concentrations, i.e., 300 (T), 600 (T), and 1,200 ppm (T) in the soil was evaluated while testing the efficiency of Crinum asiaticum L. as a phytoremedial tool. At the first assessment, i.e., 30 DAT (days after treatment) shoot and root lengths of seedlings decreased with increasing concentrations of Pb. As the study progressed, a decrease in levels of Pb was accompanied by better germinability and growth of barley. At 120 DAT seedling growth in all the treatments were comparable to control. In T, T, and T soils, 74.5%, 83.7%, and 91.2% reduction in lead content was observed at 120 DAT. Highly significant correlations between decreasing pollutant (Pb) content in the soil, seed germination, and seedling growth of barley H. vulgare were found. The differences in root and shoot length as well as overall growth pattern are indicative of the suitability of barley as a bio-monitoring tool. [ABSTRACT FROM AUTHOR]

Extracranial meningiomas are rare with a reported incidence of 1-2 %. Diagnosis is a challenge due to the unusual site of occurrence. The treatment of choice is surgical excision. A case that presented with primary lesion in the cheek with no detected intracranial extension is being reported. [ABSTRACT FROM AUTHOR]

The article focuses on a study which described the first report of ketamine-xylazine anaesthesia and its antagonism by tolazoline in captive Black buck. The study was conducted at the Nehru Zoological Park, Hyderabad, India between March 2004 and December 2006. The black bucks were withheld from food and water 24 hours prior to anaesthesia administration. After the anaesthesia administration, randomly selected animals were given yohimbine hydrochloride or tolazoline hydrochloride injection. Recovery time of the animals were recorded. It was found that a combination of ketamine-xylazine was effective in immobilising Black bucks, with the anesthetic effects reversed successfully with tolazoline.

A simple, rapid, sensitive and selective liquid chromatography / tandem mass spectrometry method was developed and validated for the quantification of pentoxifylline, a haemorheological agent. The analyte and internal standard, tamsulosin were extracted by liquid-liquid extraction with ethyl acetate and were separated using an isocratic mobile phase on a reverse phase C18 column. The analytes were analyzed by mass spectrometry in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 279/138 for pentoxifylline and m/z 409/228 for the IS. The assay exhibited a linear dynamic range of 2–1000 ng mL−1 for pentoxifylline in human plasma. The lower limit of quantification was 2 ng mL−1 with a relative standard deviation of less than 10%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 1.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. [ABSTRACT FROM AUTHOR]

A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-23082-3 [ABSTRACT FROM AUTHOR]

The interaction between surface water and groundwater is a complex process and is considered as an important component for controlling the mining activities. The objective of this study is to understand the interaction between surface water and groundwater around a proposed uranium mining site by geochemical modelling. Surface water and groundwater samples along the groundwater flow path were collected from September 2013 to June 2016 across the uranium mineralised region located near Gogi, Karnataka, India. Collected water samples were analysed for major ion and uranium concentrations. This hydrochemical data was used as input in the geochemical modelling code PHREEQC to calculate the uranium speciation and saturation indices. Inverse geochemical modelling was performed along the flow direction by considering the mineralogical composition of host rock. Measurement of surface water and groundwater level indicates that the recharge and discharge of this region were primarily controlled by rainfall. Relation between the temporal variation of rainfall and saturation index of mineral reveals the various scenarios of interaction between surface water and groundwater around the mineralised region. Silicate/carbonate weathering, irrigation return flow and dissolution of evaporites are the major processes indicated by inverse geochemical modelling, which controls the hydrogeochemical evolution of water in this region. Geochemical modelling was effectively used to understand the temporal changes in the interaction between surface water and the groundwater in a uranium mineralised region. [ABSTRACT FROM AUTHOR]