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Training load quantification methods may help optimize soccer performance. However, whole-body indicators potentially underestimate biomechanical load. A new inertial sensor setup allows joint-specific biomechanical load quantification. Good discriminative validity further supports the use of this method, and therefore the purpose of this study is to assess the discriminative validity of this method during soccer-specific activities. Twelve national and sixteen regional soccer players wore an inertial sensor setup and performed countermovement jumps, soccer kicks, and 30 m sprints. Between-group differences in angular acceleration-based biomechanical load indicators Knee Load, Hip Load, and performance were assessed using MANOVAs and Cohen's effect sizes. Furthermore, relationships with performance were explored. National players showed higher Knee Load during jumping (mean difference: 0.11 A.U., ES = 0.93, p = 0.02), kicking (mean difference: 1.94 A.U., ES = 0.94; p = 0.02), and almost during sprinting (mean difference: 12.85, ES = 0.77; p = 0.05). Hip Load did not differ between groups across all tasks, although national players outperformed regional players on all tests. Significant relationships between Knee Load (rjump = 0.41, rkick = 0.65), Hip Load (rjump = 0.42), and performance were observed with 95% confidence intervals ranging from trivial to large. The results confirm discriminative validity of Knee Load for jumping and kicking, but not for sprinting and Hip Load in general. The confidence intervals of the established relationships suggest that the biomechanical loads might not entirely explain between-group differences in performance. The results can be used as reference values for biomechanical load quantification in the field. [ABSTRACT FROM AUTHOR]

Lung transplantation is currently the gold standard treatment for end-stage lung diseases. Advances in the preservation of donor lungs, the surgical technique and immunosuppressive therapy have led to lung transplantation now being a routine procedure. Nevertheless, the shortage of donor organs, the acute and particularly chronic lung allograft dysfunction (CLAD) still represent major challenges even in experienced centers. Research in this area is still necessary to improve the long-term survival of lung recipients. [ABSTRACT FROM AUTHOR]

The emerging CdTe–BeTe semiconductor alloy that exhibits a dramatic mismatch in bond covalency and bond stiffness clarifying its vibrational-mechanical properties is used as a benchmark to test the limits of the percolation model (PM) worked out to explain the complex Raman spectra of the related but less contrasted Zn1−xBex-chalcogenides. The test is done by way of experiment ( x ≤ 0.11 ), combining Raman scattering with X-ray diffraction at high pressure, and ab initio calculations (x ~ 0–0.5; x ~1). The (macroscopic) bulk modulus B 0 drops below the CdTe value on minor Be incorporation, at variance with a linear B 0 versus x increase predicted ab initio, thus hinting at large anharmonic effects in the real crystal. Yet, no anomaly occurs at the (microscopic) bond scale as the regular bimodal PM-type Raman signal predicted ab initio for Be–Te in minority (x ~0, 0.5) is barely detected experimentally. At large Be content (x ~1), the same bimodal signal relaxes all the way down to inversion, an unprecedented case. However, specific pressure dependencies of the regular (x ~0, 0.5) and inverted (x ~1) Be–Te Raman doublets are in line with the predictions of the PM. Hence, the PM applies as such to Cd1−xBexTe without further refinement, albeit in a "relaxed" form. This enhances the model's validity as a generic descriptor of phonons in alloys. [ABSTRACT FROM AUTHOR]

The inference of genetic ancestry plays an increasingly prominent role in clinical, population, and forensic genetics studies. Several genotyping strategies and analytical methodologies have been developed over the last few decades to assign individuals to specific biogeographic regions. However, despite these efforts, ancestry inference in populations with a recent history of admixture, such as those in Brazil, remains a challenge. In admixed populations, proportion and components of genetic ancestry vary on different levels: (i) between populations; (ii) between individuals of the same population, and (iii) throughout the individual's genome. The present study evaluated 1171 admixed Brazilian samples to compare the genetic ancestry inferred by tri-/tetra-hybrid admixture models and evaluated different marker sets from those with small numbers of ancestry informative markers panels (AIMs), to high-density SNPs (HDSNP) and whole-genome-sequence (WGS) data. Analyses revealed greater variation in the correlation coefficient of ancestry components within and between admixed populations, especially for minority ancestral components. We also observed positive correlation between the number of markers in the AIMs panel and HDSNP/WGS. Furthermore, the greater the number of markers, the more accurate the tri-/tetra-hybrid admixture models. [ABSTRACT FROM AUTHOR]

Previous studies conducted mainly among adolescents have found associations between participation in sport organised leisure-time activities (OLTAs) and mental health problems (MHP). Fewer research studies have been performed to primary school-aged children and to organised non-sport OLTAs. Therefore, the objective is to examine whether there is an association between participation in sport and non-sport OLTAs and a high risk of MHP in 4- to 12-year-olds. Data were used on 5010 children from a cross-sectional population-based survey conducted between May and July 2018 in Rotterdam, the Netherlands. Associations between sport OLTAs, non-sport OLTAs and breadth of OLTAs and a high risk of MHP were explored using logistic regression models adjusting for sociodemographic characteristics, stressful life events and physical activity. Of all children, 58% participated in sport OLTAs and 22% in non-sport OLTAs. The proportion of children with high risk of MHP among participants in sport OLTAs is smaller than among non-participants (OR 0.66, 95% CI: 0.53, 0.81). The proportion of children with high risk of MHP among participants in non-sport OLTAs is smaller than among non-participants (OR 0.69, 95% CI: 0.53, 0.91). The proportion of children with a high risk of MHP among participants in 1 category of OLTAs (OR 0.61, 95% CI: 0.49, 0.76) and in 2-5 categories of OLTAs (OR 0.48, 95% CI: 0.32, 0.71) is smaller than among non-participants.  Conclusion: The proportion of children with high risk of MHP among participants in OLTAs is smaller than among non-participants. What is Known: • Around 10--20% of children and adolescents experiences mental health problems. • Sport organised leisure-time activities have been found to be associated with a lower risk of mental health problems in adolescents. What is New: • The proportion of children with a high risk of mental health problems in participants in organised leisure-time activities is smaller than among non-participants. • The proportion of children with a high risk of mental health problems in participants with a higher breadth of organised leisure-time activities is smaller compared to non-participants. [ABSTRACT FROM AUTHOR]

As whole-genome sequencing (WGS) becomes the gold standard tool for studying population genomics and medical applications, data on diverse non-European and admixed individuals are still scarce. Here, we present a high-coverage WGS dataset of 1,171 highly admixed elderly Brazilians from a census-based cohort, providing over 76 million variants, of which ~2 million are absent from large public databases. WGS enables identification of ~2,000 previously undescribed mobile element insertions without previous description, nearly 5 Mb of genomic segments absent from the human genome reference, and over 140 alleles from HLA genes absent from public resources. We reclassify and curate pathogenicity assertions for nearly four hundred variants in genes associated with dominantly-inherited Mendelian disorders and calculate the incidence for selected recessive disorders, demonstrating the clinical usefulness of the present study. Finally, we observe that whole-genome and HLA imputation could be significantly improved compared to available datasets since rare variation represents the largest proportion of input from WGS. These results demonstrate that even smaller sample sizes of underrepresented populations bring relevant data for genomic studies, especially when exploring analyses allowed only by WGS. Whole genome sequencing (WGS) data on non-European and admixed individuals remains scarce. Here, the authors analyse WGS data from 1,171 admixed elderly Brazilians from a census cohort, characterising population-specific genetic variation and exploring the clinical utility of this expanded dataset. [ABSTRACT FROM AUTHOR]

HLA-G is a promiscuous immune checkpoint molecule. The HLA-G gene presents substantial nucleotide variability in its regulatory regions. However, it encodes a limited number of proteins compared to classical HLA class I genes. We characterized the HLA-G genetic variability in 4640 individuals from 88 different population samples across the globe by using a state-of-the-art method to characterize polymorphisms and haplotypes from high-coverage next-generation sequencing data. We also provide insights regarding the HLA-G genetic diversity and a resource for future studies evaluating HLA-G polymorphisms in different populations and association studies. Despite the great haplotype variability, we demonstrated that: (1) most of the HLA-G polymorphisms are in introns and regulatory sequences, and these are the sites with evidence of balancing selection, (2) linkage disequilibrium is high throughout the gene, extending up to HLA-A, (3) there are few proteins frequently observed in worldwide populations, with lack of variation in residues associated with major HLA-G biological properties (dimer formation, interaction with leukocyte receptors). These observations corroborate the role of HLA-G as an immune checkpoint molecule rather than as an antigen-presenting molecule. Understanding HLA-G variability across populations is relevant for disease association and functional studies. [ABSTRACT FROM AUTHOR]

Copyright of Zeitschrift für Herz-, Thorax- und Gefaesschirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In the maize producing regions of Sub-Saharan Africa (SSA), compounding effects of genotype-by-environment interaction have necessitated breeding maize for outstanding performance and stability across varying environments. This study was conducted to assess the performance and stability of late-maturing cultivars and their respective hybrids evaluated under contrasting environments in the tropical rainforest region. We evaluated 108 genotypes in field trials under three different growing conditions in 2018 involving 14 open-pollinated parents and their hybrids derived from a diallel mating design. The genotypes were evaluated under field conditions using 9 × 12 alpha lattice design with three replications in six environments. The genotypes were divided into three groups, containing either the parents, hybrids or checks, for estimating the stability variance and grain yield. The difference between the lowest and highest yielding environment was 3.9 t ha−1, while the repeatability of the grain yield trials ranged from 39 to 80%. The average grain yield of the hybrids (2.33 t ha−1) was significantly higher than that of the parents (2.19 t ha−1) and the check varieties (2.03 t ha−1). The hybrids were more stable than both the parents and the checks. They also showed a higher stability against a common group of the parents and checks. The results of this study suggest that high yielding and stable population hybrids can be utilized in breeding programmes aiming to provide improved varieties for the large number of rural maize farmers in the SSA zone, who often lack access or the capacity to purchase commercial hybrids. [ABSTRACT FROM AUTHOR]

Purpose: Changes in tendon structure are commonly seen in patients with unilateral achilles (AT) or patellar (PT) tendinopathy but might also be present on the asymptomatic side, indicating a higher risk for developing symptoms. The aim of this study is to compare tendon structure of the symptomatic side with the asymptomatic side in AT and PT patients and control subjects. Methods: A total of 46 patients with unilateral AT (16 insertional and 30 midportion) and 38 with unilateral PT were included. For the control group, a total of 18 Achilles tendons and 25 patellar tendons were scanned. Tendon structure was assessed using ultrasound tissue characterisation (UTC), which quantifies tendon organisation dividing the structure into four different echo types (I–IV). Results: There were significant differences in echo types I, III, and IV between symptomatic and asymptomatic sides and controls. Additionally, there was a significant difference between the symptomatic and the asymptomatic side for all tendinopathy locations. In the insertional AT tendon portion, the symptomatic side showed a higher percentage of echo type III. For the midportion AT, the symptomatic side showed a lower percentage of echo type I and a higher percentage of echo types III and IV. For the patellar tendon, the symptomatic side showed a higher percentage of echo types III and IV. All differences were higher than the minimal detectable changes. Conclusion: Although patients have symptoms unilaterally, the tendon structures are compromised on both sides. These results stress the importance of monitoring both symptomatic and asymptomatic tendon structures and in addition highlight that the asymptomatic side should not be used as reference in clinical practice. Level of evidence: III. [ABSTRACT FROM AUTHOR]

Atmospheric methane (CH4) is a potent greenhouse gas, and its mole fraction has more than doubled since the preindustrial era1. Fossil fuel extraction and use are among the largest anthropogenic sources of CH4 emissions, but the precise magnitude of these contributions is a subject of debate2,3. Carbon-14 in CH4 (14CH4) can be used to distinguish between fossil (14C-free) CH4 emissions and contemporaneous biogenic sources; however, poorly constrained direct 14CH4 emissions from nuclear reactors have complicated this approach since the middle of the 20th century4,5. Moreover, the partitioning of total fossil CH4 emissions (presently 172 to 195 teragrams CH4 per year)2,3 between anthropogenic and natural geological sources (such as seeps and mud volcanoes) is under debate; emission inventories suggest that the latter account for about 40 to 60 teragrams CH4 per year6,7. Geological emissions were less than 15.4 teragrams CH4 per year at the end of the Pleistocene, about 11,600 years ago8, but that period is an imperfect analogue for present-day emissions owing to the large terrestrial ice sheet cover, lower sea level and extensive permafrost. Here we use preindustrial-era ice core 14CH4 measurements to show that natural geological CH4 emissions to the atmosphere were about 1.6 teragrams CH4 per year, with a maximum of 5.4 teragrams CH4 per year (95 per cent confidence limit)—an order of magnitude lower than the currently used estimates. This result indicates that anthropogenic fossil CH4 emissions are underestimated by about 38 to 58 teragrams CH4 per year, or about 25 to 40 per cent of recent estimates. Our record highlights the human impact on the atmosphere and climate, provides a firm target for inventories of the global CH4 budget, and will help to inform strategies for targeted emission reductions9,10. Isotopic evidence from ice cores indicates that preindustrial-era geological methane emissions were lower than previously thought, suggesting that present-day emissions of methane from fossil fuels are underestimated. [ABSTRACT FROM AUTHOR]

Key message: Genome-wide association on winter survival was conducted using data from image-based phenotyping method. Nine QTL were observed and three of them with candidate gene identified. Winter survival is an essential trait of winter wheat (Triticum aestivum L.) grown in regions with high risk of winterkill. We characterized a diversity panel of 450 Canadian wheat varieties that included mostly winter-growth habit wheats to identify key genetic factors that contribute to higher winter survival under field conditions. To more accurately quantify winter survival differences among varieties, image-based phenotyping methods, captured by unmanned aerial vehicle (UAV) and on ground level, were used to estimate the winter survival of each varieties. Winter survival index was developed to correct for emergence when evaluating winter survival. Winter survival measurement estimated by visual estimation, UAV imagery and ground imagery showed strong correlation with each other and had comparable broad-sense heritability. Genome-wide association studies resulted in the identification of seven quantitative trait loci (QTL) for winter survival including Vrn-A1. By using the recently released annotated sequence of the wheat genome and the available RNA-Seq data, two putative candidate genes underlying the QTL for winter survival were identified. However, our study showed that certain QTL was unique to specific winter survival measurement. Collectively, our study demonstrated the feasibility of using UAV-based imagery for the identification of loci associated with winter survival in wheat. The complexity of in-field condition make our result a valuable complement to indoor frost-tolerance studies in the identification of genetic factors not directly linked to freezing tolerance. [ABSTRACT FROM AUTHOR]

Interaction modeling is a relevant activity during software development processes. Created relying on Petri Nets theory and aiming to represent discrete time events, Colored Petri Nets (CPNs) are a graphical formal language developed and widely employed for system modeling. While traditional CPNs only have elements with ordinary stylization and behaviors, in this article we explore the key ideas behind Web Interaction Modeling Using Colored Petri Nets (wiCPN), a modeling style developed with focus on representing Web interactions as an incremental improvement of CPNs. We review wiCPN's refinements over CPNs and the modeling of the Web interface of Classroom eXperience (CX), a ubiquitous educational platform, thus verifying the model's properties to ensure it was able to represent the different access levels among its users and how wiCPN displayed suitability to comprehend this requirement on the generated model. We have also improved the originally developed model with the modification of elements to make it finite and fully analyzable. Also, we added temporization capabilities to the model and ran corresponding user simulation to observe the average time that users with different roles tend to spend during interactions. We compared wiCPN results with Unified Modeling Language (UML) Activity and Use Case diagrams, observing, as outcomes, that the generated model represented CX's interactive flow correctly and maintained a concise notation—a single wiCPN diagram was sufficient to depict the same interactive flow that, in UML, would require several diagrams, something that could overload the design team in actual software development scenarios. We also included new user experiments comprising qualitative results from experts. Finally, we created a reachability graph for the new model and generated a full state space report, analyzing Petri Nets properties such as boundedness, liveness and home marking. [ABSTRACT FROM AUTHOR]

Previously, median effective dose (ED) of 1.6 mSv per three-dimensional rotational angiography (3DRA) has been reported. This study evaluated ED and image quality in 3DRA after implementation of a simple dose reduction protocol in pediatric catheterizations. Simple conversion factors between 3DRA ED and readily available parameters at the cathlab were determined. The dose reduction protocol consisted of frame reduction (60-30 frames/s (f/s)), active collimation of the X-ray beam, usage of a readily available low dosage program, and a pre-3DRA run check. EDs were calculated with Monte Carlo PCXMC 2.0. Three observers blindly assessed 3DRA image quality of the dose reduction and normal-dose cohort. Between October 2014 and October 2015, 84 patients (median age 4.3 years) underwent 100 3DRAs with a median ED of 0.54 mSv (0.12-2.2) using the dose reduction protocol. Median ED in the normal-dose cohort (17 3DRAs) was 1.6 mSv (1.2-4.9). Image quality in the dose reduction cohort remained excellent. Correlations between ED and dose area product (DAP) and ED and skin dose were found with a ρ of 0.82 and 0.83, respectively. ED exposure of the entire catheterization was reduced to 2.64 mSv. Introduction of a simple protocol led to 66% dose reduction in 3DRA and 79% in the entire catheterization. 3DRA image quality in this group remained excellent. In 3DRA ED correlates well with DAP and skin dose, parameters readily available at the cathlab. [ABSTRACT FROM AUTHOR]

End-stage heart failure is associated with significant morbidity and mortality. Heart transplantation has the potential to offer a return to daily activities for critically ill patients and is the gold standard therapy. However, heart transplantations are decreasing yearly with a historic low in Germany in 2017. By striking contrast, both waiting list numbers and waiting time have increased owing to a lack of acceptable donor organs. Ventricular assist devices (VAD) represent a reasonable therapeutic alternative for patients on heart transplantation waiting lists. Patients ineligible for transplantation may undergo VAD implantation as a destination therapy. However, the necessity for life-long anticoagulation must be weighed against bleeding complications in potential VAD candidates. VAD-dependent patients also face risks of driveline infections, in addition to restricted activities of daily living owing to limited battery capacities. Given Germany’s low transplantation rate, VAD implantation may serve as a middle ground. With the recent events in transplantation medicine, trust among the German population has declined. Transplant centers must ensure graft quality and ongoing care, define minimum caseload for accreditation, and implement specialty care units in heart failure. Furthermore, the legislation shift from extended consent to dissent solution has the potential to end donor organ shortage. [ABSTRACT FROM AUTHOR]

Purpose: Cardiac involvement in familial transthyretin (TTR) amyloidosis is of major prognostic value, and the development of early-diagnostic tools that could trigger the use of new disease-modifying treatments is crucial. The aim of our study was to compare the respective contributions of 99mTc-diphosphonate scintigraphy (DPD, detecting amyloid deposits) and 123I-MIBG (MIBG, assessing cardiac sympathetic denervation) in patients with genetically proven TTR mutation referred for the assessment of cardiac involvement.Methods: We prospectively studied 75 consecutive patients (classified as symptomatic or asymptomatic carriers), using clinical evaluation, biomarkers (troponin and BNP), echocardiography, and nuclear imaging. Patients were classified as having normal heart-to-mediastinum (HMR) MIBG uptake ratio 4 h after injection (defined by HM4 ≥ 1.85) or abnormal HM4 < 1.85, and positive DPD uptake (grade ≥ 1 of Perugini classification) or negative DPD uptake.Results: Among 75 patients, 49 (65%) presented with scintigraphic sympathetic cardiac denervation and 29 (39%) with myocardial diphosphonate uptake. When MIBG was normal, DPD was negative except for two patients. Age was an independent predictor of abnormal scintigraphic result of both MIBG and DPD (HR 1.08 and 1.15 respectively), whereas echocardiographic-derived indicators of increased left ventricular filling pressure (E/e’ ratio) was an independent predictor of abnormal MIBG (HR 1.33) and global longitudinal strain of positive DPD (HR 1.45). In asymptomatic patients (n = 31), MIBG was abnormal in 48% (n = 15) among whom 50% had a normal DPD; all those with a normal MIBG (n = 16) had a normal DPD.Conclusions: In TTR mutation carriers, cardiac sympathetic denervation evidenced by decreased MIBG uptake is detected earlier than amyloid burden evidenced by DPD. These results raise the possibility of a diagnostic role for MIBG scintigraphy at an early stage of cardiac involvement in TTR-mutated carriers, in addition to its well-established prognostic value. [ABSTRACT FROM AUTHOR]

Growing competitive pressure forces companies to optimise process productivity and shorten primary production times. At the same time, the resulting manufacturing quality must be kept on a high level. In the automotive sector, deep hole drilling with smallest tool diameters is an important process, e.g. to produce lubrication holes in crankshafts and fuel channels in injectors. A crucial criterion for the achievable productivity and manufacturing quality with respect to the dimensional and shape tolerances as well as the surface quality in smallest diameter deep hole drilling is the chip formation. Therefore, in-depth analyses regarding the mechanisms of chip formation at the cutting edge and the chip removal along the chip flutes are indispensable. To accomplish an indepth chip formation analysis in smallest diameter deep hole drilling, a new methodology of analysis has been developed. Samples made of the particular test material are inserted into acrylic glass carriers, and the chip formation in the operating zone and the chip removal are documented by high-speed microscopy. In this paper, the experimental setup of the newly developed methodology of analysis and the experimental results for single-lip and twist deep hole drilling of high-strength bainitic steel with smallest diameters are shown. The investigations show the dependence of chip formation on the changes of the microstructure of the cutting edge due to tool wear, and form the basis for an optimization of the tools. In addition to that, a new approach to visualise machining processes running under non-transparent coolant is presented. [ABSTRACT FROM AUTHOR]

Activities of specification, analysis and design of real-time systems (RTS) are highly dependent on an effective understanding of the application domain and on the thorough representation of their basic requirements. Model-based approaches using modeling languages such as UML are often applied to contribute to handle complexity of RTS development. However, UML alone does not completely represent important features associated with these systems, such as relationship with hardware elements and an effective representation of timing constraints. This article explores the combined use of UML and its profiles SysML and MARTE for modeling hardware and software requirements of RTS, with application to a case of controlling urban road traffic. The SysML profile alone does not present the representation of temporal, behavioral and performance requirements. The MARTE profile provides key resources to specify non-functional requirements for RTS, in addition to a clear description of the various relevant aspects of requirements definition of RTS, as for instance, temporal aspects and constraints. The main objective is to present the combined application of SysML with MARTE stereotypes, which enables the specification of different features of individual software requirements. Thus, in addition to the factors mentioned above, we can say that the proposed approach has an important role to specify RTS at different levels of detail and levels of abstraction. [ABSTRACT FROM AUTHOR]

In this paper, we propose models, algorithms, and implementation details of an approach that extract the most relevant entity rankings from large datasets. This is done in a fully automated way, as with large amounts of structured data, beyond well understood databases (schemas), manual solutions do not scale. The core task of our approach is to decide which categorical constraints, ranking order (descending or ascending), and length form together an interesting ranking. We make use of a model based on information entropy to find interesting/relevant categorical constraints and devise pruning conditions to avoid generating too many irrelevant rankings. We further investigate the skewness of the value distributions of ranking criteria to find suitable ranking dimensions and ranking order, and present an overall scoring model to assess the meaningfulness of a ranking. For each individual step of our approach, we discuss iterative MapReduce-based algorithms. Finally, the experimental evaluation on real-world data is reported where the users manually evaluate our approach of generating most relevant rankings. [ABSTRACT FROM AUTHOR]

Copyright of Manuelle Medizin is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Schizophrenia is a complex, multifactorial disease that displays heterogeneous behavioral and cognitive syndrome (Lieberman & First, 2018). The origin of schizophrenia appears to lie in genetic and/or environmental disruption of brain development (Owen et al, 2016). In spite of current treatment that largely consists in antipsychotic drugs combined with psychological therapies, social support, and rehabilitation, developing more effective therapeutic interventions is an essential issue.

Background:High-level microsatellite instability (MSI-H) has been reported as a prognostic marker in colon cancer. We here analysed the prognostic significance of MSI and mutations of the Beta2-Microglobulin (B2M) gene, which occur in about 30% of MSI-H colon cancer, in the cohort of the prospective FOGT-4 (Forschungsruppe Onkologie Gastrointestinale Tumoren, FOGT) trial.Methods:Microsatellite instability status was determined using standard protocols (NCI/ICG-HNPCC panel and CAT25) in 223 colon cancer lesions. Beta2-Microglobulin mutation status was evaluated by exon-wise sequencing in all MSI-H lesions.Results:Patients with MSI-H (n=34) colon cancer presented with a significantly lower risk of relapse after 12 months of follow-up compared with MSS (n=189) colon cancer patients (5 year time to relapse: MSI-H 0.82 vs MSS 0.66, P=0.03). No significant difference in overall survival was detected. Beta2-Microglobulin mutations were identified in 10 (29.4%) out of 34 MSI-H colon cancers and were associated with a complete absence of disease relapse or tumour-related death events (P=0.09).Conclusion:The risk of late disease relapse was significantly lower in patients with MSI-H compared with MSS colon cancer. Moreover, B2M mutations may contribute to the favourable outcome of MSI-H colon cancer patients and should therefore be evaluated as a potential prognostic marker in future clinical trials. [ABSTRACT FROM AUTHOR]