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In 2019, a vast oil spill reached more than 4,000 km of the Brazilian coast, leading to an urgent action, which included the assessment of seafood safety from the affected areas. The aim of our study was the development, optimization, validation, and application of a fast, sensible, and reliable method to determine eight polycyclic aromatic hydrocarbons in seafood. The sample preparation was based on pressurized liquid extraction using a hard cap coffee machine and subsequent quantitation by liquid chromatography coupled to tandem mass spectrometry with atmospheric-pressure chemical ionization source (LC-APCI-MS/MS). The method performance was verified in accordance to the Commission Decision 2002/657/EC in terms of selectivity, linearity (R2 > 0.95), precision (CV < 21%), accuracy (75–115%), decision limit, and detection capability. The analytical limits were set to 0.9–1.25 µg kg−1 (limits of detection) and 1.25–5.00 µg kg−1 (limits of quantitation). The method was applied to more than a hundred of seafood samples. Concentrations of benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]anthracene, benzo[ghi]perylene, and indene[1,2,3-cd]pyrene ranged from < LOQ to 156 µg kg−1. Benzo[a]anthracene (41.7%), benzo[a]pyrene (41.7%), and benzo[b]fluoranthene (37,5%) were the most frequent PAH, ranging from < LOQ to 113 µg kg−1. The analyzed samples showed PAH concentrations below the concern levels established by the Brazilian regulatory agency and below the limits established by European Union. Risk assessment was performed to evaluate the daily dietary intake of seafood and the carcinogenic potential of the measured levels, based on Brazilian seafood consumption profile, PAHs levels, and toxicity equivalent factors. [ABSTRACT FROM AUTHOR]

Certain feedingstuffs are sources of contamination for animals with cadmium (Cd) and lead (Pb). Different approaches for sample digestion, performance assessment, and measurement uncertainty for the analysis of Pb and Cd by flame atomic absorption spectrometry were compared. Full method validation, including matrix effect and analytical limits, were assessed. Despite having a lower performance compared to the microwave digestion, dry ashing was a viable alternative for sample digestion, leading to lower operating costs. Interlaboratory validation proved to be a cheaper alternative, instead of the long traditional in-house approach. The method was suitable for routine analysis of several feed samples. There was a greater amount of quantifiable results for Pb, ranging from traces up to high concentrations (7430 ± 930 mg kg). Cd was also quantified in some samples, ranging from 0.11 ± 0.01 to 8.71 ± 0.90 mg kg. The higher concentrations of both metals were found in mineral mixtures and fish meal. [ABSTRACT FROM AUTHOR]