34 results on '"Moons, Lieve"'
Search Results
2. Tissue stretching is a confounding factor for the evaluation of neurodegeneration in the fast-ageing killifish.
- Author
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Bergmans, Steven, Serneels, Pieter-Jan, Masin, Luca, and Moons, Lieve
- Abstract
The fast-ageing killifish has gained increasing attention as a promising gerontology model to study age-related processes and neurodegeneration. Interestingly, it is the first vertebrate model organism that shows physiological neuron loss at old age in its central nervous system (CNS), including its brain and retina. However, the fact that the killifish brain and retina are ever-growing tissues complicates studying neurodegenerative events in aged fish. Indeed, recent studies showed that the method of tissue sampling, either using sections or whole-organs, has a large effect on the observed cell densities in the fast-expanding CNS. Here, we elaborated on how these two sampling methods affect neuronal counts in the senescent retina and how this tissue grows upon ageing. Analysis of the different retinal layers in cryosections revealed age-dependent reduction in cellular density but evaluation of whole-mount retinas did not detect any neuron loss, as a result of an extremely fast retinal expansion with age. Using BrdU pulse-chase experiments, we showed that the young adult killifish retina mainly grows by cell addition. However, with increasing age, the neurogenic potency of the retina declines while the tissue keeps on growing. Further histological analyses revealed tissue stretching, including cell size increase, as the main driver of retinal growth at old age. Indeed, both cell size and inter-neuronal distance augment with ageing, thereby decreasing neuronal density. All in all, our findings urge the 'ageing science' community to consider cell quantification bias and employ tissue-wide counting methods to reliably quantify neuronal numbers in this unique gerontology model. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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3. The killifish visual system as an in vivo model to study brain aging and rejuvenation.
- Author
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Vanhunsel, Sophie, Bergmans, Steven, Beckers, An, Etienne, Isabelle, Van houcke, Jolien, Seuntjens, Eve, Arckens, Lut, De Groef, Lies, and Moons, Lieve
- Published
- 2021
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4. Corticotropin-releasing factor induces functional and structural synaptic remodelling in acute stress.
- Author
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Vandael, Dorien, Wierda, Keimpe, Vints, Katlijn, Baatsen, Pieter, De Groef, Lies, Moons, Lieve, Rybakin, Vasily, and Gounko, Natalia V.
- Published
- 2021
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5. Long-term plasticity of inhibitory synapses in the hippocampus and spatial learning depends on matrix metalloproteinase 3.
- Author
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Wiera, Grzegorz, Lebida, Katarzyna, Lech, Anna Maria, Brzdąk, Patrycja, Van Hove, Inge, De Groef, Lies, Moons, Lieve, Petrini, Enrica Maria, Barberis, Andrea, and Mozrzymas, Jerzy W.
- Subjects
CONTEXTUAL learning ,MAZE tests ,SYNAPSES ,LONG-term potentiation ,NEUROPLASTICITY ,HIPPOCAMPUS (Brain) ,DIFFUSION coefficients - Abstract
Learning and memory are known to depend on synaptic plasticity. Whereas the involvement of plastic changes at excitatory synapses is well established, plasticity mechanisms at inhibitory synapses only start to be discovered. Extracellular proteolysis is known to be a key factor in glutamatergic plasticity but nothing is known about its role at GABAergic synapses. We reveal that pharmacological inhibition of MMP3 activity or genetic knockout of the Mmp3 gene abolishes induction of postsynaptic iLTP. Moreover, the application of exogenous active MMP3 mimics major iLTP manifestations: increased mIPSCs amplitude, enlargement of synaptic gephyrin clusters, and a decrease in the diffusion coefficient of synaptic GABA
A receptors that favors their entrapment within the synapse. Finally, we found that MMP3 deficient mice show faster spatial learning in Morris water maze and enhanced contextual fear conditioning. We conclude that MMP3 plays a key role in iLTP mechanisms and in the behaviors that presumably in part depend on GABAergic plasticity. [ABSTRACT FROM AUTHOR]- Published
- 2021
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6. A novel retinal ganglion cell quantification tool based on deep learning.
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Masin, Luca, Claes, Marie, Bergmans, Steven, Cools, Lien, Andries, Lien, Davis, Benjamin M., Moons, Lieve, and De Groef, Lies
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RETINAL ganglion cells ,GLAUCOMA ,OCULAR hypertension ,DEEP learning ,RETINAL diseases - Abstract
Glaucoma is a disease associated with the loss of retinal ganglion cells (RGCs), and remains one of the primary causes of blindness worldwide. Major research efforts are presently directed towards the understanding of disease pathogenesis and the development of new therapies, with the help of rodent models as an important preclinical research tool. The ultimate goal is reaching neuroprotection of the RGCs, which requires a tool to reliably quantify RGC survival. Hence, we demonstrate a novel deep learning pipeline that enables fully automated RGC quantification in the entire murine retina. This software, called RGCode (Retinal Ganglion Cell quantification based On DEep learning), provides a user-friendly interface that requires the input of RBPMS-immunostained flatmounts and returns the total RGC count, retinal area and density, together with output images showing the computed counts and isodensity maps. The counting model was trained on RBPMS-stained healthy and glaucomatous retinas, obtained from mice subjected to microbead-induced ocular hypertension and optic nerve crush injury paradigms. RGCode demonstrates excellent performance in RGC quantification as compared to manual counts. Furthermore, we convincingly show that RGCode has potential for wider application, by retraining the model with a minimal set of training data to count FluoroGold-traced RGCs. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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7. Single-cell transcriptome analysis of the Akimba mouse retina reveals cell-type-specific insights into the pathobiology of diabetic retinopathy.
- Author
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Van Hove, Inge, De Groef, Lies, Boeckx, Bram, Modave, Elodie, Hu, Tjing-Tjing, Beets, Karen, Etienne, Isabelle, Van Bergen, Tine, Lambrechts, Diether, Moons, Lieve, Feyen, Jean H. M., and Porcu, Michaël
- Abstract
Aims/hypothesis: Diabetic retinopathy is a common complication of diabetes and a leading cause of visual impairment and blindness. Despite recent advances, our understanding of its pathophysiology remains incomplete. The aim of this study was to provide deeper insight into the complex network of molecular and cellular changes that underlie diabetic retinopathy by systematically mapping the transcriptional changes that occur in the different cellular compartments of the degenerating diabetic mouse retina. Methods: Single-cell RNA sequencing was performed on retinal tissue from 12-week-old wild-type and Akimba (Ins2
Akita ×Vegfa+/– ) mice, which are known to replicate features of clinical diabetic retinopathy. This resulted in transcriptome data for 9474 retinal cells, which could be annotated to eight distinct retinal cell types. Using STRING analysis, we studied differentially expressed gene networks in neuronal, glial and immune cell compartments to create a comprehensive view on the pathological changes that occur in the Akimba retina. Using subclustering analysis, we further characterised macroglial and inflammatory cell subpopulations. Prominent findings were confirmed at the protein level using immunohistochemistry, western blotting and ELISA. Results: At 12 weeks, the Akimba retina was found to display degeneration of rod photoreceptors and presence of inflammatory cells, identified by subclustering analysis as monocyte, macrophage and microglial populations. Analysis of differentially expressed genes in the rod, cone, bipolar cell and macroglial compartments indicated changes in cell metabolism and ribosomal gene expression, gliosis, activation of immune system pathways and redox and metal ion dyshomeostasis. Experiments at the protein level supported a metabolic shift from glycolysis to oxidative phosphorylation (glyceraldehyde 3-phosphate dehydrogenase), activation of microglia/macrophages (isolectin-B4), metal ion and oxidative stress response (metallothionein and haem oxygenase-1) and reactive macroglia (glial fibrillary acidic protein and S100) in the Akimba retina, compared with wild-type mice. Our single-cell approach also indicates macroglial subpopulations with distinct fibrotic, inflammatory and gliotic profiles. Conclusions/interpretation: Our study identifies molecular pathways underlying inflammatory, metabolic and oxidative stress-mediated changes in the Akimba mouse model of diabetic retinopathy and distinguishes distinct functional subtypes of inflammatory and macroglial cells. Data availability: RNA-seq data have been deposited in the ArrayExpress database at EMBL-EBI (www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-9061. [ABSTRACT FROM AUTHOR]- Published
- 2020
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8. Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina.
- Author
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Das, Yannick, Roose, Nele, De Groef, Lies, Fransen, Marc, Moons, Lieve, Van Veldhoven, Paul P., and Baes, Myriam
- Abstract
The retinal pathology in peroxisomal disorders suggests that peroxisomes are important to maintain retinal homeostasis and function. These ubiquitous cell organelles are mainly involved in lipid metabolism, which comprises α- and β-oxidation and ether lipid synthesis. Although peroxisomes were extensively studied in liver, their role in the retina still remains to be elucidated. As a first step in gaining more insight into the role of peroxisomes in retinal physiology, we performed immunohistochemical stainings, immunoblotting and enzyme activity measurements to reveal the distribution of peroxisomes and peroxisomal lipid metabolizing enzymes in the murine retina. Whereas peroxisomes were detected in every retinal layer, we found a clear differential distribution of the peroxisomal lipid metabolizing enzymes in the neural retina compared to the retinal pigment epithelium. In particular, the ABC transporters that transfer lipid substrates into the organelle as well as several enzymes of the β-oxidation pathway were enriched either in the neural retina or in the retinal pigment epithelium. In conclusion, our results strongly indicate that peroxisome function varies between different regions in the murine retina. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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9. An Antagonistic Axon-Dendrite Interplay Enables Efficient Neuronal Repair in the Adult Zebrafish Central Nervous System.
- Author
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Beckers, An, Van Dyck, Annelies, Bollaerts, Ilse, Van houcke, Jessie, Lefevere, Evy, Andries, Lien, Agostinone, Jessica, Van Hove, Inge, Di Polo, Adriana, Lemmens, Kim, and Moons, Lieve
- Abstract
Neural insults and neurodegenerative diseases typically result in permanent functional deficits, making the identification of novel pro-regenerative molecules and mechanisms a primary research topic. Nowadays, neuroregenerative research largely focuses on improving axonal regrowth, leaving the regenerative properties of dendrites largely unstudied. Moreover, whereas developmental studies indicate a strict temporal separation of axogenesis and dendritogenesis and thus suggest a potential interdependency of axonal and dendritic outgrowth, a possible axon-dendrite interaction during regeneration remains unexplored. To unravel the inherent dendritic response of vertebrate neurons undergoing successful axonal regeneration, regeneration-competent adult zebrafish of either sex, subjected to optic nerve crush (ONC), were used. A longitudinal study in which retinal ganglion cell (RGC) dendritic remodeling and axonal regrowth were assessed side-by-side after ONC, revealed that—as during development—RGC axogenesis precedes dendritogenesis during central nervous system (CNS) repair. Moreover, dendrites majorly shrank before the start of axonal regrowth and were only triggered to regrow upon RGC target contact initiation, altogether suggestive for a counteractive interplay between axons and dendrites after neuronal injury. Strikingly, both retinal mechanistic target of rapamycin (mTOR) and broad-spectrum matrix metalloproteinase (MMP) inhibition after ONC consecutively inhibited RGC synapto-dendritic deterioration and axonal regrowth, thus invigorating an antagonistic interplay wherein mature dendrites restrain axonal regrowth. Altogether, this work launches dendritic shrinkage as a prerequisite for efficient axonal regrowth of adult vertebrate neurons, and indicates that molecular/mechanistic analysis of dendritic responses after damage might represent a powerful target-discovery platform for neural repair. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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10. Retinal α-synuclein deposits in Parkinson's disease patients and animal models.
- Author
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Veys, Lien, Vandenabeele, Marjan, Ortuño-Lizarán, Isabel, Baekelandt, Veerle, Cuenca, Nicolás, Moons, Lieve, and De Groef, Lies
- Subjects
PARKINSON'S disease ,EXTRAPYRAMIDAL disorders ,RETINAL ganglion cells - Abstract
Despite decades of research, accurate diagnosis of Parkinson's disease remains a challenge, and disease-modifying treatments are still lacking. Research into the early (presymptomatic) stages of Parkinson's disease and the discovery of novel biomarkers is of utmost importance to reduce this burden and to come to a more accurate diagnosis at the very onset of the disease. Many have speculated that non-motor symptoms could provide a breakthrough in the quest for early biomarkers of Parkinson's disease, including the visual disturbances and retinal abnormalities that are seen in the majority of Parkinson's disease patients. An expanding number of clinical studies have investigated the use of in vivo assessments of retinal structure, electrophysiological function, and vision-driven tasks as novel means for identifying patients at risk that need further neurological examination and for longitudinal follow-up of disease progression in Parkinson's disease patients. Often, the results of these studies have been interpreted in relation to α-synuclein deposits and dopamine deficiency in the retina, mirroring the defining pathological features of Parkinson's disease in the brain. To better understand the visual defects seen in Parkinson's disease patients and to propel the use of retinal changes as biomarkers for Parkinson's disease, however, more conclusive neuropathological evidence for the presence of retinal α-synuclein aggregates, and its relation to the cerebral α-synuclein burden, is urgently needed. This review provides a comprehensive and critical overview of the research conducted to unveil α-synuclein aggregates in the retina of Parkinson's disease patients and animal models, and thereby aims to aid the ongoing discussion about the potential use of the retinal changes and/or visual symptoms as biomarkers for Parkinson's disease. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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11. Extensive growth is followed by neurodegenerative pathology in the continuously expanding adult zebrafish retina.
- Author
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Van houcke, Jessie, Geeraerts, Emiel, Vanhunsel, Sophie, Beckers, An, Noterdaeme, Lut, Christiaens, Marijke, Bollaerts, Ilse, De Groef, Lies, and Moons, Lieve
- Abstract
The development of effective treatments for age-related neurodegenerative diseases remains one of the biggest medical challenges today, underscoring the high need for suitable animal model systems to improve our understanding of aging and age-associated neuropathology. Zebrafish have become an indispensable complementary model organism in gerontology research, yet their growth-control properties significantly differ from those in mammals. Here, we took advantage of the clearly defined and highly conserved structure of the fish retina to study the relationship between the processes of growth and aging in the adult zebrafish central nervous system (CNS). Detailed morphological measurements reveal an early phase of extensive retinal growth, where both the addition of new cells and stretching of existent tissue drive the increase in retinal surface. Thereafter, and coinciding with a significant decline in retinal growth rate, a neurodegenerative phenotype becomes apparent,-characterized by a loss of synaptic integrity, an age-related decrease in cell density and the onset of cellular senescence. Altogether, these findings support the adult zebrafish retina as a valuable model for gerontology research and CNS disease modeling and will hopefully stimulate further research into the mechanisms of aging and age-related pathology. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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12. Resistance to retinopathy development in obese, diabetic and hypertensive ZSF1 rats: an exciting model to identify protective genes.
- Author
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Caolo, Vincenza, Roblain, Quentin, Lecomte, Julie, Carai, Paolo, Peters, Linsey, Cuijpers, Ilona, Robinson, Emma Louise, Derks, Kasper, Sergeys, Jurgen, Noël, Agnès, Jones, Elizabeth A. V., Moons, Lieve, and Heymans, Stephane
- Published
- 2018
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13. Complementary research models and methods to study axonal regeneration in the vertebrate retinofugal system.
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Bollaerts, Ilse, Veys, Lien, Geeraerts, Emiel, Andries, Lien, Buyens, Tom, Salinas-Navarro, Manuel, Moons, Lieve, De Groef, Lies, and Van Hove, Inge
- Subjects
OPTIC nerve injuries ,CENTRAL nervous system regeneration ,AXONS ,LABORATORY rodents ,LABORATORY zebrafish ,IN vitro studies ,IN vivo studies - Abstract
Due to the lack of axonal regeneration, age-related deterioration in the central nervous system (CNS) poses a significant burden on the wellbeing of a growing number of elderly. To overcome this regenerative failure and to improve the patient’s life quality, the search for novel regenerative treatment strategies requires valuable (animal) models and techniques. As an extension of the CNS, the retinofugal system, consisting of retinal ganglion cells that send their axons along the optic nerve to the visual brain areas, has importantly contributed to the current knowledge on mechanisms underlying the restricted regenerative capacities and to the development of novel strategies to enhance axonal regeneration. It provides an extensively used research tool, not only in amniote vertebrates including rodents, but also in anamniote vertebrates, such as zebrafish. Indeed, the latter show robust regeneration capacities, thereby providing insights into the factors that contribute to axonal regrowth and proper guidance, complementing studies in mammals. This review provides an integrative and critical overview of the classical and state-of-the-art models and methods that have been employed in the retinofugal system to advance our knowledge on the signaling pathways underlying the restricted versus robust axonal regeneration in rodents and zebrafish, respectively. In vitro, ex vivo and in vivo models and techniques to improve the visualization and analysis of regenerating axons are summarized. As such, the retinofugal system is presented as a valuable model to further facilitate research on axonal regeneration and to open novel therapeutic avenues for CNS pathologies. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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14. Characterizing microglia activation: a spatial statistics approach to maximize information extraction.
- Author
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Davis, Benjamin M., Salinas-Navarro, Manual, Cordeiro, M. Francesca, Moons, Lieve, and De Groef, Lies
- Abstract
Microglia play an important role in the pathology of CNS disorders, however, there remains significant uncertainty about the neuroprotective/degenerative role of these cells due to a lack of techniques to adequately assess their complex behaviour in response to injury. Advancing microscopy techniques, transgenic lines and well-characterized molecular markers, have made histological assessment of microglia populations more accessible. However, there is a distinct lack of tools to adequately extract information from these images to fully characterise microglia behaviour. This, combined with growing economic pressures and the ethical need to minimise the use of laboratory animals, led us to develop tools to maximise the amount of information obtained. This study describes a novel approach, combining image analysis with spatial statistical techniques. In addition to monitoring morphological parameters and global changes in microglia density, nearest neighbour distance, and regularity index, we used cluster analyses based on changes in soma size and roundness to yield novel insights into the behaviour of different microglia phenotypes in a murine optic nerve injury model. These methods should be considered a generic tool to quantitatively assess microglia activation, to profile phenotypic changes into microglia subpopulations, and to map spatial distributions in virtually every CNS region and disease state. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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15. Matrix Metalloproteinases During Axonal Regeneration, a Multifactorial Role from Start to Finish.
- Author
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Andries, Lien, Van Hove, Inge, Moons, Lieve, and De Groef, Lies
- Abstract
By proteolytic cleavage, matrix metalloproteinases (MMPs) not only remodel the extracellular matrix (ECM) but they also modify the structure and activity of other proteinases, growth factors, signaling molecules, cell surface receptors, etc. Their vast substrate repertoire adds a complex extra dimension of biological control and turns MMPs into important regulatory nodes in the protease web. In the central nervous system (CNS), the detrimental impact of elevated MMP activities has been well-described for traumatic injuries and many neurodegenerative diseases. Nonetheless, there is ample proof corroborating MMPs as fine regulators of CNS physiology, and well-balanced MMP activity is instrumental to development, plasticity, and repair. In this manuscript, we review the emerging evidence for MMPs as beneficial modulators of axonal regeneration in the mammalian CNS. By exploring the multifactorial causes underlying the inability of mature axons to regenerate, and describing how MMPs can help to overcome these hurdles, we emphasize the benign actions of these Janus-faced proteases. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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16. Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants.
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Buyens, Tom, Gaublomme, Djoere, Van Hove, Inge, De Groef, Lies, and Moons, Lieve
- Published
- 2014
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17. Altered neuronal architecture and plasticity in the visual cortex of adult MMP-3-deficient mice.
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Aerts, Jeroen, Nys, Julie, Moons, Lieve, Hu, Tjing-Tjing, and Arckens, Lutgarde
- Subjects
NEUROPLASTICITY ,EXTRACELLULAR matrix proteins ,VISUAL cortex ,MATRIX metalloproteinases ,ENDOPEPTIDASES ,LABORATORY mice - Abstract
Matrix metalloproteinases (MMPs) are Zn-dependent endopeptidases considered to be essential for normal brain development and neuroplasticity by modulating extracellular matrix proteins, receptors, adhesion molecules, growth factors and cytoskeletal proteins. Specifically, MMP-3 has recently been implicated in synaptic plasticity, hippocampus-dependent learning and neuronal development and migration in the cerebellum. However, the function(s) of this enzyme in the neocortex is understudied. Therefore, we explored the phenotypical characteristics of the neuronal architecture and the capacity for experience-dependent cortical plasticity in the visual cortex of adult MMP-3-deficient (MMP-3) mice. Golgi-Cox stainings revealed a significant reduction in apical dendritic length and an increased number of apical obliques for layer V pyramidal neurons in the visual cortex of adult MMP-3 mice compared to wild-type (WT) animals. In addition, a significant upregulation of both phosphorylated and non-phosphorylated neurofilament protein (NF)-high, phosphorylated NF-medium, NF-low and α-internexin was detected in the visual cortex of MMP-3 mice. To assess the effect of MMP-3 deficiency on cortical plasticity, we monocularly enucleated adult MMP-3 mice and analyzed the reactivation of the contralateral visual cortex 7 weeks post-enucleation. In contrast to previous results in C57Bl/6J adult mice, activity remained confined to the binocular zone and did not expand into the monocular regions indicative for an aberrant open-eye potentiation. Permanent hypoactivity in the monocular cortex lateral and medial to V1 also indicated a lack of cross-modal plasticity. These observations demonstrate that genetic inactivation of MMP-3 has profound effects on the structural integrity and plasticity response of the visual cortex of adult mice. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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18. MMP-2 mediates Purkinje cell morphogenesis and spine development in the mouse cerebellum.
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Verslegers, Mieke, Van Hove, Inge, Dekeyster, Eline, Gantois, Ilse, Hu, Tjing-Tjing, D'Hooge, Rudi, Arckens, Lutgarde, and Moons, Lieve
- Subjects
MATRIX metalloproteinases ,PURKINJE cells ,SPINE ,CEREBELLUM ,LABORATORY mice ,PROTEOLYTIC enzymes ,BRAIN function localization - Abstract
Matrix metalloproteinase-2 (MMP-2) is a highly studied proteolytic enzyme, involved in many detrimental and beneficial functions throughout the body, and also active in the central nervous system (CNS). MMP-2 is profoundly expressed in the developing cerebellum and was recently reported to modulate granule cell proliferation by affecting cell cycle kinetics in cerebella of postnatal day 3 mouse pups. In this report, a two-dimensional difference gel electrophoresis proteomics study was implemented at this postnatal stage and revealed 16 differentially expressed proteins between MMP-2-deficient (MMP-2) and wild-type cerebella. Among those, collapsin response mediator protein 1 (CRMP1) could be identified as the most significant differential protein between the two genotypes. Western blot experiments confirmed this finding and further disclosed a significant increase in phosphorylated CRMP1 expression in MMP-2 cerebella. Strikingly, subsequent immunohistochemical and microscopic analyses revealed an aberrant Purkinje cell (PC) dendritogenesis, possibly related to upregulated (phospho-) CRMP1 levels in these neonatal MMP-2 animals. Further, detailed morphometric analyses showed persistent PC morphological changes in MMP-2 mice, from the neonatal stage until adulthood. These were characterized by a reduced growth of PC somata, reduced dendritic tree sizes, and a decreased dendritic arborization. During development, the observed defects were accompanied by a temporarily disturbed parallel fiber and climbing fiber synaptic input on the PCs, while in adult MMP-2 animals, an increased PC spine density and reduced spine lengths were noted. The observed PC abnormalities might contribute to the mild defects in motor performance, i.e. balance and coordination, detected in adult MMP-2 mice. Overall, these findings indicate the importance of MMP-2 in CNS development and dendritogenesis, and highlight the importance of a correct developmental wiring for adult brain morphology and function. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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19. A multidisciplinary approach unravels early and persistent effects of X-ray exposure at the onset of prenatal neurogenesis.
- Author
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Verreet, Tine, Quintens, Roel, Van Dam, Debby, Verslegers, Mieke, Tanori, Mirella, Casciati, Arianna, Neefs, Mieke, Leysen, Liselotte, Michaux, Arlette, Janssen, Ann, D'Agostino, Emiliano, Velde, Greetje Vande, Baatout, Sarah, Moons, Lieve, Pazzaglia, Simonetta, Saran, Anna, Himmelreich, Uwe, De Deyn, Peter Paul, and Benotmane, Mohammed Abderrafi
- Abstract
Background In humans, in utero exposure to ionising radiation results in an increased prevalence of neurological aberrations, such as small head size, mental retardation and decreased IQ levels. Yet, the association between early damaging events and long-term neuronal anomalies remains largely elusive. Methods Mice were exposed to different X-ray doses, ranging between 0.0 and 1.0 Gy, at embryonic days (E) 10, 11 or 12 and subjected to behavioural tests at 12 weeks of age. Underlying mechanisms of irradiation at E11 were further unravelled using magnetic resonance imaging (MRI) and spectroscopy, diffusion tensor imaging, gene expression profiling, histology and immunohistochemistry. Results Irradiation at the onset of neurogenesis elicited behavioural changes in young adult mice, dependent on the timing of exposure. As locomotor behaviour and hippocampal-dependent spatial learning and memory were most particularly affected after irradiation at E11 with 1.0 Gy, this condition was used for further mechanistic analyses, focusing on the cerebral cortex and hippocampus. A classical p53-mediated apoptotic response was found shortly after exposure. Strikingly, in the neocortex, the majority of apoptotic and microglial cells were residing in the outer layer at 24 h after irradiation, suggesting cell death occurrence in differentiating neurons rather than proliferating cells. Furthermore, total brain volume, cortical thickness and ventricle size were decreased in the irradiated embryos. At 40 weeks of age, MRI showed that the ventricles were enlarged whereas N-acetyl aspartate concentrations and functional anisotropy were reduced in the cortex of the irradiated animals, indicating a decrease in neuronal cell number and persistent neuroinflammation. Finally, in the hippocampus, we revealed a reduction in general neurogenic proliferation and in the amount of Sox2-positive precursors after radiation exposure, although only at a juvenile age. Conclusions Our findings provide evidence for a radiation-induced disruption of mouse brain development, resulting in behavioural differences. We propose that alterations in cortical morphology and juvenile hippocampal neurogenesis might both contribute to the observed aberrant behaviour. Furthermore, our results challenge the generally assumed view of a higher radiosensitivity in dividing cells. Overall, this study offers new insights into irradiation-dependent effects in the embryonic brain, of relevance for the neurodevelopmental and radiobiological field. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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20. Vascular and Neuronal Effects of VEGF in the Nervous System.
- Author
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Janigro, Damir, Moons, Lieve, Carmeliet, Peter, and Dewerchin, Mieke
- Abstract
Blood vessels and nerves are both vital channels to and from tissues. Recent genetic insights show that they have much more in common than was originally anticipated. They use similar signals and principles to differentiate, grow, and navigate toward their targets. Moreover, the vascular and nervous systems cross talk and, when dysregulated, this contributes to medically important diseases. No factor is better known for its angiogenic effects than vascular endothelial growth factor (VEGF)—this molecule has been implicated in virtually every type of angiogenic disorder, including those associated with cancer, ischemia, and inflammation. Recent studies revealed, however, that VEGF is also involved in neurodegeneration. The role of VEGF in the nervous system is not restricted only to regulating vessel growth: VEGF also has direct effects on different types of neural cells—including even neural stem cells. Furthermore, genetic studies showed that mice with reduced VEGF levels develop adult-onset motor neuron degeneration reminiscent of the human neurodegenerative disorder amyotrophic lateral sclerosis (ALS), and additional genetic studies confirmed that VEGF is a modifier of motor neuron degeneration in humans and in SOD1G93A mice—a model of ALS. Reduced VEGF levels may promote motor neuron degeneration by limiting neural tissue perfusion, by reducing VEGF-dependent neuroprotection and/or by influencing neuroregeneration/neurogenesis. VEGF also affects neuron survival after acute spinal cord or cerebral ischemia and has been implicated in other neurological disorders such as diabetic and ischemic neuropathy, nerve regeneration, Parkinson's disease, Alzheimer's disease, and multiple sclerosis. These findings offer novel opportunities to better decipher the insufficiently understood molecular pathogenesis of many neurodegenerative disorders, and promise to open new avenues for future improved treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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21. An Aberrant Cerebellar Development in Mice Lacking Matrix Metalloproteinase-3.
- Author
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Van Hove, Inge, Verslegers, Mieke, Buyens, Tom, Delorme, Nathalie, Lemmens, Kim, Stroobants, Stijn, Gantois, Ilse, D'Hooge, Rudi, and Moons, Lieve
- Abstract
Cell-cell and cell-matrix interactions are necessary for neuronal patterning and brain wiring during development. Matrix metalloproteinases (MMPs) are proteolytic enzymes capable of remodelling the pericellular environment and regulating signaling pathways through cleavage of a large degradome. MMPs have been suggested to affect cerebellar development, but the specific role of different MMPs in cerebellar morphogenesis remains unclear. Here, we report a role for MMP-3 in the histogenesis of the mouse cerebellar cortex. MMP-3 expression peaks during the second week of postnatal cerebellar development and is most prominently observed in Purkinje cells (PCs). In MMP-3 deficient (MMP-3) mice, a protracted granule cell (GC) tangential migration and a delayed GC radial migration results in a thicker and persistent external granular layer, a retarded arrival of GCs in the inner granular layer, and a delayed GABAergic interneuron migration. Importantly, these neuronal migration anomalies, as well as the consequent disturbed synaptogenesis on PCs, seem to be caused by an abnormal PC dendritogenesis, which results in reduced PC dendritic trees in the adult cerebellum. Of note, these developmental and adult cerebellar defects might contribute to the aberrant motor phenotype observed in MMP-3 mice and suggest an involvement of MMP-3 in mouse cerebellar development. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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22. Modeling lymphangiogenesis in a three-dimensional culture system.
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Bruyère, Françoise, Melen-Lamalle, Laurence, Blacher, Silvia, Roland, Guy, Thiry, Marc, Moons, Lieve, Frankenne, Francis, Carmeliet, Peter, Alitalo, Kari, Libert, Claude, Sleeman, Jonathan P., Foidart, Jean-Michel, and Noël, Agnès
- Subjects
NEOVASCULARIZATION ,CELL proliferation ,ELECTRON microscopy ,LABORATORY rats ,CELLS - Abstract
A lack of appropriate in vitro models of three-dimensional lymph vessel growth hampers the study of lymphangiogenesis. We developed a lymphatic ring assay—a potent, reproducible and quantifiable three-dimensional culture system for lymphatic endothelial cells that reproduces spreading of endothelial cells from a pre-existing vessel, cell proliferation, migration and differentiation into capillaries. In the assay, mouse thoracic duct fragments are embedded in a collagen gel, leading to the formation of lumen-containing lymphatic capillaries, which we assessed by electron microscopy and immunostaining. We developed a computerized method to quantify the lymphatic network. By applying this model to gene-deficient mice, we found evidence for involvement of the matrix metalloproteinase, MMP-2, in lymphangiogenesis. The lymphatic ring assay bridges the gap between two-dimensional in vitro models and in vivo models of lymphangiogenesis, can be used to exploit the potential of existing transgenic mouse models, and rapidly identify regulators of lymphangiogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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23. Treatment of motoneuron degeneration by intracerebroventricular delivery of VEGF in a rat model of ALS.
- Author
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Storkebaum, Erik, Lambrechts, Diether, Dewerchin, Mieke, Moreno-Murciano, Maria-Paz, Appelmans, Saskia, Oh, Hideyasu, Van Damme, Philip, Rutten, Bart, Man, Wing Yan, De Mol, Maria, Wyns, Sabine, Manka, David, Vermeulen, Kristel, Van Den Bosch, Ludo, Mertens, Nico, Schmitz, Christoph, Robberecht, Wim, Conway, Edward M, Collen, D&aeacute;sir&aeacute;, and Moons, Lieve
- Subjects
AMYOTROPHIC lateral sclerosis ,DISEASES ,NEURODEGENERATION ,NEUROMUSCULAR diseases ,CYTOKINES ,MYONEURAL junction - Abstract
Neurotrophin treatment has so far failed to prolong the survival of individuals affected with amyotrophic lateral sclerosis (ALS), an incurable motoneuron degenerative disorder. Here we show that intracerebroventricular (i.c.v.) delivery of recombinant vascular endothelial growth factor (Vegf) in a SOD1
G93A rat model of ALS delays onset of paralysis by 17 d, improves motor performance and prolongs survival by 22 d, representing the largest effects in animal models of ALS achieved by protein delivery. By protecting cervical motoneurons, i.c.v. delivery of Vegf is particularly effective in rats with the most severe form of ALS with forelimb onset. Vegf has direct neuroprotective effects on motoneurons in vivo, because neuronal expression of a transgene expressing the Vegf receptor prolongs the survival of SOD1G93A mice. On i.c.v. delivery, Vegf is anterogradely transported and preserves neuromuscular junctions in SOD1G93A rats. Our findings in preclinical rodent models of ALS may have implications for treatment of neurodegenerative disease in general. [ABSTRACT FROM AUTHOR]- Published
- 2005
- Full Text
- View/download PDF
24. Role of PlGF in the intra- and intermolecular cross talk between the VEGF receptors Flt1 and Flk1.
- Author
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Autiero, Monica, Waltenberger, Johannes, Communi, Didier, Kranz, Andrea, Moons, Lieve, Lambrechts, Diether, Kroll, Jens, Plaisance, Stephane, De Mol, Maria, Bono, Francoise, Kliche, Stefanie, Fellbrich, Guido, Ballmer-Hofer, Kurt, Maglione, Domenico, Mayr-Beyrle, Ulrike, Dewerchin, Mieke, Dombrowski, Saskia, Stanimirovic, Danica, and Van Hummelen, Paul
- Subjects
NEOVASCULARIZATION ,VASCULAR endothelium ,GROWTH factors - Abstract
Therapeutic angiogenesis is likely to require the administration of factors that complement each other. Activation of the receptor tyrosine kinase (RTK) Flk1 by vascular endothelial growth factor (VEGF) is crucial, but molecular interactions of other factors with VEGF and Flk1 have been studied to a limited extent. Here we report that placental growth factor (PGF, also known as PIGF) regulates inter- and intramolecular cross talk between the VEGF RTKs Flt1 and Flk1. Activation of Flt1 by PGF resulted in intermolecular transphosphorylation of Flk1, thereby amplifying VEGF-driven angiogenesis through Flk1. Even though VEGF and PGF both bind Flt1, PGF uniquely stimulated the phosphorylation of specific Flt1 tyrosine residues and the expression of distinct downstream target genes. Furthermore, the VEGF/PGF heterodimer activated intramolecular VEGF receptor cross talk through formation of Flk1/Flt1 heterodimers. The inter- and intramolecular VEGF receptor cross talk is likely to have therapeutic implications, as treatment with VEGF/PGF heterodimer or a combination of VEGF plus PGF increased ischemic myocardial angiogenesis in a mouse model that was refractory to VEGF alone. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
25. Revascularization of ischemic tissues by PlGF treatment, and inhibition of tumor angiogenesis, arthritis and atherosclerosis by anti-Flt1.
- Author
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Luttun, Aernout, Tjwa, Marc, Moons, Lieve, Wu, Yan, Angelillo-Scherrer, Anne, Liao, Fang, Nagy, Janice A., Hooper, Andrea, Priller, Josef, De Klerck, Bert, Compernolle, Veerle, Daci, Evis, Bohlen, Peter, Dewerchin, Mieke, Herbert, Jean-Marc, Fava, Roy, Matthys, Patrick, Carmeliet, Geert, and Collen, Désiré
- Subjects
NEOVASCULARIZATION inhibitors ,BLOOD-vessel development ,MYOCARDIUM ,VASCULAR endothelium - Abstract
The therapeutic potential of placental growth factor (PlGF) and its receptor Flt1 in angiogenesis is poorly understood. Here, we report that PlGF stimulated angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to vascular endothelial growth factor (VEGF). An antibody against Flt1 suppressed neovascularization in tumors and ischemic retina, and angiogenesis and inflammatory joint destruction in autoimmune arthritis. Anti-Flt1 also reduced atherosclerotic plaque growth and vulnerability, but the atheroprotective effect was not attributable to reduced plaque neovascularization. Inhibition of VEGF receptor Flk1 did not affect arthritis or atherosclerosis, indicating that inhibition of Flk1-driven angiogenesis alone was not sufficient to halt disease progression. The anti-inflammatory effects of anti-Flt1 were attributable to reduced mobilization of bone marrow?derived myeloid progenitors into the peripheral blood; impaired infiltration of Flt1-expressing leukocytes in inflamed tissues; and defective activation of myeloid cells. Thus, PlGF and Flt1 constitute potential candidates for therapeutic modulation of angiogenesis and inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2002
- Full Text
- View/download PDF
26. Loss of HIF-2α and inhibition of VEGF impair fetal lung maturation, whereas treatment with VEGF prevents fatal respiratory distress in premature mice.
- Author
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Compernolle, Veerle, Brusselmans, Koen, Acker, Till, Hoet, Peter, Tjwa, Marc, Beck, Heike, Plaisance, Stéphane, Dor, Yuval, Keshet, Eli, Lupu, Florea, Nemery, Benoit, Dewerchin, Mieke, Van Veldhoven, Paul, Plate, Karl, Moons, Lieve, Collen, Désiré, and Carmeliet, Peter
- Subjects
HYPOXEMIA ,OBSTRUCTIVE lung diseases ,PREMATURE infants ,TRANSCRIPTION factors - Abstract
Respiratory distress syndrome (RDS) due to insufficient production of surfactant is a common and severe complication of preterm delivery. Here, we report that loss of the hypoxia-inducible transcription factor-2α (HIF-2α) caused fatal RDS in neonatal mice due to insufficient surfactant production by alveolar type 2 cells. VEGF, a target of HIF-2α, regulates fetal lung maturation: because VEGF levels in alveolar cells were reduced in HIF-2α-deficient fetuses; mice with a deficiency of the VEGF
164 and VEGF188 isoforms or of the HIF-binding site in the VEGF promotor died of RDS; intrauterine delivery of anti-VEGF-receptor-2 antibodies caused RDS and VEGF stimulated production of surfactant proteins by cultured type 2 pneumocytes. Intrauterine delivery or postnatal intratracheal instillation of VEGF stimulated conversion of glycogen to surfactant and protected preterm mice against RDS. The pneumotrophic effect of VEGF may have therapeutic potential for lung maturation in preterm infants. [ABSTRACT FROM AUTHOR]- Published
- 2002
- Full Text
- View/download PDF
27. Abrupt rate accelerations or premature beats cause life-threatening arrhythmias in mice with long-QT3 syndrome.
- Author
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Nuyens, Dieter, Stengl, Milan, Dugarmaa, Saran, Rossenbacker, Tom, Compernolle, Veerle, Rudy, Yoram, Smits, Jos F., Flameng, Willem, Clancy, Colleen E., Moons, Lieve, Vos, Marc A., Dewerchin, Mieke, Benndorf, Klaus, Collen, Désiré, Carmeliet, Edward, and Carmeliet, Peter
- Subjects
ARRHYTHMIA ,HEART beat ,LABORATORY mice - Abstract
Reports that abrupt heart rate acceleration or premature beats cause life threatening arrhythmias in mice with long-QT3 syndrome. Description of arrhythmias; Anti-arrhythmic property of andrenergic agonists.
- Published
- 2001
- Full Text
- View/download PDF
28. Deletion of the hypoxia-response element in the vascular endothelial growth factor promoter causes motor neuron degeneration.
- Author
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Oosthuyse, Bert, Moons, Lieve, Storkebaum, Erik, Beck, Heike, Nuyens, Dieter, Brusselmans, Koen, Dorpe, Jo Van, Hellings, Peter, Gorselink, Marchel, Heymans, Stéphane, Theilmeier, Gregor, Dewerchin, Mieke, Laudenbach, Vincent, Vermylen, Patrick, Raat, Harold, Acker, Till, Vleminckx, Vicky, Bosch, Ludo Van Den, and Cashman, Neil
- Subjects
- *
NEURODEGENERATION , *VASCULAR endothelium , *SPINAL cord , *HYPOXEMIA - Abstract
Hypoxia stimulates angiogenesis through the binding of hypoxia-inducible factors to the hypoxia-response element in the vascular endothelial growth factor (Vegf) promotor. Here, we report that deletion of the hypoxia-response element in the Vegf promotor reduced hypoxic Vegf expression in the spinal cord and caused adult-onset progressive motor neuron degeneration, reminiscent of amyotrophic lateral sclerosis. The neurodegeneration seemed to be due to reduced neural vascular perfusion. In addition, Vegf165 promoted survival of motor neurons during hypoxia through binding to Vegf receptor 2 and neuropilin 1. Acute ischemia is known to cause nonselective neuronal death. Our results indicate that chronic vascular insufficiency and, possibly, insufficient Vegf-dependent neuroprotection lead to the select degeneration of motor neurons. [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
29. Synergism between vascular endothelial growth factor and placental growth factor contributes to angiogenesis and plasma extravasation in pathological conditions.
- Author
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Carmeliet, Peter, Moons, Lieve, Luttun, Aernout, Vincenti, Valeria, Compernolle, Veerle, De Mol, Maria, Wu, Yan, Bono, Françoise, Devy, Laetitia, Beck, Heike, Scholz, Dimitri, Acker, Till, DiPalma, Tina, Dewerchin, Mieke, Noel, Agnes, Stalmans, Ingeborg, Barra, Adriano, Blacher, Sylvia, and Vandendriessche, Thierry
- Subjects
- *
GROWTH factors , *NEOVASCULARIZATION , *BONE marrow , *CYTOKINES - Abstract
Vascular endothelial growth factor (VEGF) stimulates angiogenesis by activating VEGF receptor-2 (VEGFR-2). The role of its homolog, placental growth factor (PlGF), remains unknown. Both VEGF and PlGF bind to VEGF receptor-1 (VEGFR-1), but it is unknown whether VEGFR-1, which exists as a soluble or a membrane-bound type, is an inert decoy or a signaling receptor for PlGF during angiogenesis. Here, we report that embryonic angiogenesis in mice was not affected by deficiency of PlGF (Pgf-/-). VEGF-B, another ligand of VEGFR-1, did not rescue development in Pgf-/- mice. However, loss of PlGF impaired angiogenesis, plasma extravasation and collateral growth during ischemia, inflammation, wound healing and cancer. Transplantation of wild-type bone marrow rescued the impaired angiogenesis and collateral growth in Pgf-/- mice, indicating that PlGF might have contributed to vessel growth in the adult by mobilizing bone-marrow?derived cells. The synergism between PlGF and VEGF was specific, as PlGF deficiency impaired the response to VEGF, but not to bFGF or histamine. VEGFR-1 was activated by PlGF, given that anti-VEGFR-1 antibodies and a Src-kinase inhibitor blocked the endothelial response to PlGF or VEGF/PlGF. By upregulating PlGF and the signaling subtype of VEGFR-1, endothelial cells amplify their responsiveness to VEGF during the 'angiogenic switch' in many pathological disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
30. Impaired myocardial angiogenesis and ischemic cardiomyopathy in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188.
- Author
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Carmeliet, Peter, Ng, Yin-Shan, Nuyens, Dieter, Theilmeier, Gregor, Brusselmans, Koen, Cornelissen, Ivo, Ehler, Elisabeth, Kakkar, Vijay V., Stalmans, Ingeborg, Mattot, Virginie, Perriard, Jean-Claude, Dewerchin, Mieke, Flameng, Willem, Nagy, Andras, Lupu, Florea, Moons, Lieve, Collen, Désiré, D'Amore, Patricia A., and Shima, David T.
- Subjects
NEOVASCULARIZATION ,CARDIOMYOPATHIES - Abstract
Focuses on impaired myocardial angiogenesis and ischemic cardiomyopathy in mice lacking the vascular endothelial growth factor isoforms VEGF. Generation of VEGF in mice; Impaired cardiac performance; Impaired myocardial angiogenesis.
- Published
- 1999
- Full Text
- View/download PDF
31. Role of HIF-1 alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis.
- Author
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Carmeliet, Peter, Dor, Yuval, Herbert, Jean-Marc, Fukumura, Dai, Brusselmans, Koen, Dewerchin, Mieke, Neeman, Michal, Bono, Françoise, Abramovitch, Rinat, Maxwell, Patrick, Koch, Cameron J., Ratcliffe, Peter, Moons, Lieve, Jain, Rakesh K., Collen, Désiré, and Keshet, Eli
- Subjects
HYPOXEMIA ,HYPOGLYCEMIA ,EMBRYONIC stem cells ,CELL proliferation -- Molecular aspects ,APOPTOSIS ,MOLECULES ,PHYSIOLOGY - Abstract
Presents research which showed that hypoxia and hypoglycemia reduce proliferation and increase apoptosis in wild-type embryonic stem (ES) cells. Role of hypoxia-inducible factor (HIF)-1-alpha in oxygen homeostasis; Effects in ES cells with inactivated HIF-1-alpha genes; Effects of a deficiency in HIF-1-alpha; Role of hypoxia/hypoglycemia-regulated genes; Different adaptive responses to loss of oxygen and nutrients.
- Published
- 1998
- Full Text
- View/download PDF
32. Urokinase-generated plasmin activates matrix metalloproteinases during aneurysm formation.
- Author
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Carmeliet, Peter, Moons, Lieve, Lijnen, Roger, Baes, Myriam, Lemaître, Vincent, Tipping, Peter, Drew, Angela, Eeckhout, Yves, Shapiro, Steve, Lupu, Florea, and Collen, Désiré
- Published
- 1997
- Full Text
- View/download PDF
33. A novel serine protease inhibitor as potential treatment for dry eye syndrome and ocular inflammation.
- Author
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Joossen, Cedric, Baán, Adrienn, Moreno-Cinos, Carlos, Joossens, Jurgen, Cools, Nathalie, Lanckacker, Ellen, Moons, Lieve, Lemmens, Kim, Lambeir, Anne-Marie, Fransen, Erik, Delputte, Peter, Caljon, Guy, Van Der Veken, Pieter, Maes, Louis, De Meester, Ingrid, Kiekens, Filip, Augustyns, Koen, and Cos, Paul
- Subjects
DRY eye syndromes ,SERINE proteinases ,PROTEASE inhibitors ,FLUORESCEIN ,CYCLOSPORINE - Abstract
Dry eye syndrome (DES), a multifactorial disorder which leads to ocular discomfort, visual disturbance and tear film instability, has a rising prevalence and limited treatment options. In this study, a newly developed trypsin-like serine protease inhibitor (UAMC-00050) in a tear drop formulation was evaluated to treat ocular inflammation. A surgical animal model of dry eye was employed to investigate the potential of UAMC-00050 on dry eye pathology. Animals treated with UAMC-00050 displayed a significant reduction in ocular surface damage after evaluation with sodium fluorescein, compared to untreated, vehicle treated and cyclosporine-treated animals. The concentrations of IL-1α and TNF-α were also significantly reduced in tear fluid from UAMC-00050-treated rats. Additionally, inflammatory cell infiltration in the palpebral conjunctiva (CD3 and CD45), was substantially reduced. An accumulation of pro-MMP-9 and a decrease in active MMP-9 were found in tear fluid from animals treated with UAMC-00050, suggesting that trypsin-like serine proteases play a role in activating MMP-9 in ocular inflammation in this animal model. Comparative qRT-PCR analyses on ocular tissue indicated the upregulation of tryptase, urokinase plasminogen activator receptor (uPAR) and protease-activated receptor 2 (PAR2). The developed UAMC-00050 formulation was stable up to 6 months at room temperature in the absence of light, non-irritating and sterile with compatible pH and osmolarity. These results provide a proof-of-concept for the in vivo modifying potential of UAMC-00050 on dry eye pathology and suggest a central role of trypsin-like serine proteases and PAR2 in dry eye derived ocular inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
34. Impaired myocardial angiogenesis and ischemic cardiomyopathy in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188.
- Author
-
Carmeliet, Peter, Ng, Yin-Shan, Nuyens, Dieter, Theilmeier, Gregor, Brusselmans, Koen, Cornelissen, Ivo, Ehler, Elisabeth, Kakkar, Vijay V., Stalmans, Ingeborg, Mattot, Virginie, Perriard, Jean-Claude, Dewerchin, Mieke, Flameng, Willem, Nagy, Andras, Lupu, Florea, Moons, Lieve, Collen, Désiré, D'Amore, Patricia A., and Shima, David T.
- Subjects
- *
NEOVASCULARIZATION , *CARDIOMYOPATHIES - Abstract
Focuses on impaired myocardial angiogenesis and ischemic cardiomyopathy in mice lacking the vascular endothelial growth factor isoforms VEGF. Generation of VEGF in mice; Impaired cardiac performance; Impaired myocardial angiogenesis.
- Published
- 1999
- Full Text
- View/download PDF
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