1. The Hop2 and Mnd1 proteins act in concert with Rad51 and Dmc1 in meiotic recombination.
- Author
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Petukhova, Galina V., Pezza, Roberto J., Vanevski, Filip, Ploquin, Mickael, Masson, Jean-Yves, and Camerini-Otero, R. Daniel
- Subjects
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MEIOSIS , *CELL division , *GENETIC recombination , *CHROMOSOMES , *CELL nuclei , *CELL proliferation - Abstract
During the first meiotic division, homologous chromosomes (homologs) have to separate to opposite poles of the cell to ensure the right complement in the progeny. Homologous recombination provides a mechanism for a genome-wide homology search and physical linkage among the homologs before their orderly segregation. Rad51 and Dmc1 recombinases are the major players in these processes. Disruption of meiosis-specific HOP2 or MND1 genes leads to severe defects in homologous synapsis and an early-stage recombination failure resulting in sterility. Here we show that mouse Hop2 can efficiently form D-loops, the first recombination intermediates, but this activity is abrogated upon association with Mnd1. Furthermore, the Hop2-Mnd1 heterodimer physically interacts with Rad51 and Dmc1 recombinases and stimulates their activity up to 35-fold. Our data reveal an interplay among Hop2, Mnd1 and Rad51 and Dmc1 in the formation of the first recombination intermediates during meiosis. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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