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Your search keyword '"Polywka, Susanne"' showing total 3 results
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3 results on '"Polywka, Susanne"'

1. Autoantibodies against 'rods and rings'-related IMPDH2 in hepatitis C genotype 1 and DAA therapy in a 'real life' cohort.

Author

Dammermann, Werner, Polywka, Susanne, Dettmann, Inga, Mindorf, Swantje, Komorowski, Lars, Wehmeyer, Malte, Schulze zur Wiesch, Julian, Stöcker, Winfried, and Lüth, Stefan

Subjects
*HEPATITIS C, *ANTIVIRAL agents, *AUTOANTIBODIES, *DEHYDROGENASES, *VIROLOGY, *IMMUNOLOGY
Abstract

Autoantibodies against inosine-5′-monophosphate-dehydrogenase-2 (IMPDH2; 'rods and rings' pattern) develop in chronic hepatitis C (CHC) patients under treatment with peg-interferon (IFN) and ribavirin (RBV), an inhibitor of IMPDH2. We investigated the influence of the alternative therapy with direct-acting antivirals (DAA)/ribavirin on anti-IMPDH2 autoantibody generation and the use of anti-IMPDH2 development as a marker for therapy outcome (sustained virologic response, SVR). We analyzed a 'real life' cohort of 104 unselected CHC genotype 1 (GT1) patients treated with IFN/first-generation DAA/RBV prospectively compared to a historic cohort of 59 IFN/RBV-treated CHC GT1 patients. First-generation DAA were boceprevir (BOC) or telaprevir (TPR). Serum autoantibodies were tested by indirect immunofluorescence (IFA) using recombinant IMPDH2 expressing HEK293 cells and native HEp2-cells as substrates. 64/163 (39%) CHC patients turned anti-IMPDH2 positive during therapy, but only 43/163 (26%) showed also 'rods and rings' structures. 99/163 (61%) were tested as anti-IMPDH2 negative. 53/104 (51%) CHC patients undergoing IFN/DAA/RBV therapy were anti-IMPDH2 positive and 38/104 (37%) were in parallel anti-'rods and rings' positive. HCV clearance/SVR rate after IFN/DAA/RBV therapy and anti-IMPDH2 status were not significantly dependent. CHC GT1 patients treated with IFN/first-generation DAA/RBV developed anti-IMPDH2 autoantibodies comparable to previous studies including patients under IFN/RBV therapy. Anti-IMPDH2 titers show no use as a marker for therapy outcome in CHC GT1 patients. [ABSTRACT FROM AUTHOR]

Published
2017
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2. A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay.

Author

Walker, John M., Zhu, Tuofu, Polywka, Susanne, Duttmann, Hedwig, Lübben, Frank, Laufs, Rainer, and Feldner, Jürgen

Abstract

It is important to shorten the window period after acute HIV infection in which infected individuals are still antibody-negative, especially in blood donors. Newly developed fourth-generation assays detect antibodies to HIV-1, including subtype O, and to HIV-2 and, simultaneously, p24 antigen of HIV-1. To evaluate this assay for daily routine work we compared it with different third-generation assays using sera from uninfected patients and patients with known HIV infection. The most interesting sera are those drawn during seroconversion from freshly infected patients. Whenever we encounter such a patient with acute HIV infection we store the serum in aliquots at −20°C. Thus, we were able to establish our own seroconversion panel and use it in our laboratory for evaluation of new assays. The new test was shown to be able to detect all chronically HIV-infected individuals and four of six patients during seroconversion although in two of these patients conventional assays for HIV antibodies were still negative. The rate of unspecific reactivities was slightly higher as compared with third-generation assays. [ABSTRACT FROM AUTHOR]

Published
2005
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3. Close correlation between hepatitis C virus serology and polymerase chain reaction in chronically infected patients.

Author

Reuter, D., Polywka, Susanne, Feucht, H., Laufs, R., and Iske, L.

Abstract

Copyright of Infection is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
1992
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