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Studies on workplace incivility have typically posited that the targets of incivility respond in a "tit-for-tat" manner. Moving beyond this dominant logic, we argue that in some cases, the targets may have a different response to incivility that potentially reduces its spiraling negative consequences. Drawing on attribution theory, we explored the following two aspects of the targets' responses: psychological motivation and subsequent behavioral response. Based on 555 samples of experience sampling data collected from 61 nurses over 10 workdays at a hospital in China, we found that the nurses' attribution of incivility to either the uncivil patient or themselves moderated the relationship between the patients' incivility and the employees' psychological motivation. When the nurses attributed the reason for a patient's incivility to the patient, their experience of incivility triggered their revenge motivation. In contrast, when the nurses attributed to themselves the reason for a patient's incivility, their experience of incivility triggered their forgiveness motivation. Furthermore, we found that the nurses' revenge motivation positively affected their subsequent incivility toward third parties, whereas their forgiveness motivation positively promoted their subsequent helping behavior toward third parties. This study enriches the application of attribution theory to the spillover effects of incivility toward third parties. [ABSTRACT FROM AUTHOR]

Objective: Portal hypertension leads to hepatic artery dilatation and a higher risk of bleeding. We tried to identify the bleeding risk after gastroesophageal varices (GOV) treatment using hepatic artery diameter of contrast-enhanced CT. Methods: Retrospective retrieval of 258 patients with cirrhosis who underwent contrast-enhanced CT from January 2022 to May 2023 and endoscopy within one month thereafter at Hainan Affiliated Hospital of Hainan Medical University. Cirrhotic patients before GOV treatment were used as the test cohort (n = 199), and cirrhotic patients after GOV treatment were used as the validation cohort (n = 59). The grading and bleeding risk was classified according to the endoscopic findings. Arterial-phase images of contrast-enhanced CT were used for coronal reconstruction, and the midpoint diameter of the hepatic artery was measured on coronal images. The optimal cutoff value for identifying bleeding risk was analyzed and calculated in the test cohort, and its diagnostic performance was evaluated in the validation cohort. Results: In the test cohort, hepatic artery diameters were significantly higher in high-risk GOV than in low-risk GOV [4.69 (4.31, 5.56) vs. 3.10 (2.59, 3.77), P < 0.001]. With a hepatic artery diameter cutoff value of 4.06 mm, the optimal area under the operating characteristic curve was 0.940 (95% confidence interval: 0.908–0.972), with a sensitivity of 0.887, a specificity of 0.892, a positive predictive value of 0.904, and a negative predictive value of 0.874 for identifying bleeding risk in the test cohort, while in the validation cohort, the sensitivity was 0.885, specificity was 0.939, positive predictive value was 0.920, and negative predictive value was 0.912. Conclusion: Hepatic artery diameter has high diagnostic performance in identifying bleeding risk after GOV treatment. [ABSTRACT FROM AUTHOR]

Prostate cancer (PCa) is a commonly occurring malignancy in elderly men. Olaparib, a poly ADP-ribose polymerase inhibitor, is utilized in PCa treatment. However, patients often develop resistance to olaparib after a period of treatment. Genetic alterations may play a significant role in this resistance, but the specific genes involved remain unclear. This study collected RNA-sequence data from the Gene Expression Omnibus database on both olaparib-sensitive and -resistant PCa cells to identify genes crucial for resistance. Subsequently, the enriched pathways of these genes were analyzed, and a protein–protein interaction (PPI) network was constructed to identify hub genes. The effect of these hub genes on PCa occurrence, progression, and prognosis was assessed using data from The Cancer Genome Atlas and Chinese Prostate Cancer Genome and Epigenome Atlas databases. Finally, this study validated our findings in clinical PCa samples and cells. From the GSE189186 dataset, 50 upregulated genes and 2 downregulated genes were identified in olaparib-resistant C4-2B and LNCaP cells. Utilizing the PPI network, eight upregulated genes (BBC3, TP53I3, FDXR, DDB2, CDKN1A, GADD45A, ZMAT3, and SESN1) were identified as hub genes for olaparib-resistant PCa cells. Furthermore, some of these genes were central to PCa occurrence, with BBC3 also influencing progression and prognosis. Importantly, BBC3 expression was upregulated in clinical PCa samples and affected PCa cells sensitive to olaparib, suggesting its potential as a predictive marker for PCa development and olaparib resistance. [ABSTRACT FROM AUTHOR]

Superconductivity in a highly correlated kagome system has been theoretically proposed for years (refs. 1–5), yet the experimental realization is hard to achieve6,7. The recently discovered vanadium-based kagome materials8, which exhibit both superconductivity9–11 and charge-density-wave orders12–14, are nonmagnetic8,9 and weakly correlated15,16. Thus these materials are unlikely to host the exotic superconductivity theoretically proposed. Here we report the discovery of a chromium-based kagome metal, CsCr3Sb5, which is contrastingly featured with strong electron correlations, frustrated magnetism and characteristic flat bands close to the Fermi level. Under ambient pressure, this kagome metal undergoes a concurrent structural and magnetic phase transition at 55 K, with a stripe-like 4a0 structural modulation. At high pressure, the phase transition evolves into two transitions, possibly associated with charge-density-wave and antiferromagnetic spin-density-wave orderings. These density-wave-like orders are gradually suppressed with pressure and, remarkably, a superconducting dome emerges at 3.65–8.0 GPa. The maximum of the superconducting transition temperature, Tcmax = 6.4 K, appears when the density-wave-like orders are completely suppressed at 4.2 GPa, and the normal state exhibits a non-Fermi-liquid behaviour, reminiscent of unconventional superconductivity and quantum criticality in iron-based superconductors17,18. Our work offers an unprecedented platform for investigating superconductivity in correlated kagome systems.The emergence of superconductivity under pressure is reported in a chromium-based kagome metal, CsCr3Sb5, which has strong electron correlations, frustrated magnetism and characteristic flat bands close to the Fermi level. [ABSTRACT FROM AUTHOR]

Takemoto's catalysts were used to organocatalyze the enantioselective Friedel–Crafts reaction with different electron-rich phenols and substituted isatins. The resulting 3-aryl-3-hydroxyl-2-oxindoles were obtained in good yields (85–96%) with up to 99% ee. The substrate scope was broadened with this methodology compared to reported examples catalyzed by cinchonidine thiourea. [ABSTRACT FROM AUTHOR]

Purpose: To evaluate the diagnostic performance of contrast-enhanced (CE) ultrasound using Sonazoid (SNZ–CEUS) by comparing with contrast-enhanced computed tomography (CE–CT) and contrast-enhanced magnetic resonance imaging (CE–MRI) for differentiating benign and malignant renal masses. Materials and methods: 306 consecutive patients (from 7 centers) with renal masses (40 benign tumors, 266 malignant tumors) diagnosed by both SNZ–CEUS, CE–CT or CE–MRI were enrolled between September 2020 and February 2021. The examinations were performed within 7 days, but the sequence was not fixed. Histologic results were available for 301 of 306 (98.37%) lesions and 5 lesions were considered benign after at least 2 year follow-up without change in size and image characteristics. The diagnostic performances were evaluated by sensitivity, specificity, positive predictive value, negative predictive value, and compared by McNemar's test. Results: In the head-to-head comparison, SNZ–CEUS and CE–MRI had comparable sensitivity (95.60 vs. 94.51%, P = 0.997), specificity (65.22 vs. 73.91%, P = 0.752), positive predictive value (91.58 vs. 93.48%) and negative predictive value (78.95 vs. 77.27%); SNZ–CEUS and CE–CT showed similar sensitivity (97.31 vs. 96.24%, P = 0.724); however, SNZ–CEUS had relatively lower than specificity than CE–CT (59.09 vs. 68.18%, P = 0.683). For nodules > 4 cm, CE–MRI demonstrated higher specificity than SNZ–CEUS (90.91 vs. 72.73%, P = 0.617) without compromise the sensitivity. Conclusions: SNZ–CEUS, CE–CT, and CE–MRI demonstrate desirable and comparable sensitivity for the differentiation of renal mass. However, the specificity of all three imaging modalities is not satisfactory. SNZ–CEUS may be a suitable alternative modality for patients with renal dysfunction and those allergic to gadolinium or iodine-based agents. Key Points: This prospective multicenter study demonstrated that the diagnostic performance of SNZ–CEUS was comparable to CE–CT and CE–MRI. The three imaging modalities displayed desirable sensitivity, while the specificity needs to be further improved. CE–MRI may have better specificity than SNZ–CEUS for differentiating renal masses bigger than 4 cm [ABSTRACT FROM AUTHOR]

Red flesh apple (Malus pumila var. medzwetzkyana Dieck), purple leaf plum (Prunus cerasifera Ehrhar f), and purple leaf peach (Prunus persica 'Atropurpurea') are significant ornamental plants within the Rosaceae family. The coloration of their fruits and leaves is crucial in their appearance and nutritional quality. However, qualitative and quantitative studies on flavonoids in the succulent fruits and leaves of multicolored Rosaceae plants are lacking. To unveil the diversity and variety-specificity of flavonoids in these three varieties, we conducted a comparative analysis of flavonoid metabolic components using ultra-high-performance liquid phase mass spectrometry (UPLC-MS/MS). The results revealed the detection of 311 metabolites, including 47 flavonoids, 105 flavonols, 16 chalcones, 37 dihydroflavonoids, 8 dihydroflavonols, 30 anthocyanins, 14 flavonoid carbon glycosides, 23 flavanols, 8 isoflavones, 11 tannins, and 12 proanthocyanidins. Notably, although the purple plum and peach leaves exhibited distinct anthocyanin compounds, paeoniflorin and corythrin glycosides were common but displayed varying glycosylation levels. While the green purple leaf peach fruit (PEF) and red flesh apple leaf (AL) possessed the lowest anthocyanin content, they exhibited the highest total flavonoid content. Conversely, the red flesh apple fruit (AF) displayed the highest anthocyanin content and a diverse range of anthocyanin glycosylation modifications, indicating that anthocyanins predominantly influenced the fruit's color. Purple PLF, PLL, and PEL showcased varying concentrations of anthocyanins, suggesting that their colors result from the co-color interaction between specific types of anthocyanins and secondary metabolites, such as flavonols, flavonoids, and dihydroflavonoids. This study provides novel insights into the variations in tissue metabolites among Rosaceae plants with distinct fruit and leaf colors. [ABSTRACT FROM AUTHOR]

Malus baccata (L.) var. gracilis (Rehd.) has high ornamental value and breeding significance, and comparative chloroplast genome analysis was applied to facilitate genetic breeding for desired traits and resistance and provide insight into the phylogeny of this genus. Using data from whole-genome sequencing, a tetrameric chloroplast genome with a length of 159,992 bp and a total GC content of 36.56% was constructed. The M. baccata var. gracilis chloroplast genome consists of a large single-copy sequence (88,100 bp), a short single-copy region (19,186 bp), and two inverted repeat regions, IRa (26,353 bp) and IRb (26,353 bp). This chloroplast genome contains 112 annotated genes, including 79 protein-coding genes (nine multicopy), 29 tRNA genes (eight multicopy), and four rRNA genes (all multicopy). Calculating the relative synonymous codon usage revealed a total of 32 high-frequency codons, and the codons exhibited a biased usage pattern towards A/U as the ending nucleotide. Interspecific sequence comparison and boundary analysis revealed significant sequence variation in the vast single-copy region, as well as generally similar expansion and contraction of the SSC and IR regions for 10 analyzed Malus species. M. baccata var. gracilis and Malus hupehensis were grouped together into one branch based on phylogenetic analysis of chloroplast genome sequences. The chloroplast genome of Malus species provides an important foundation for species identification, genetic diversity analysis, and Malus chloroplast genetic engineering. Additionally, the results can facilitate the use of pendant traits to improve apple tree shape. [ABSTRACT FROM AUTHOR]

Drawing upon conservation of resources theory, we develop and test a theoretical model that specifies how and when employee humor toward leaders affects leader abusive supervision. We propose that employee humor is negatively associated with leader abusive supervision via leader relational energy. Furthermore, the negative indirect relationship between employee humor and leader abusive supervision via leader relational energy is stronger for female leaders than for male leaders. An experiment and a multi-wave, multi-source field study provide substantial support for our hypotheses. Our findings contribute to the abusive supervision literature by identifying employee humor as a safe and effective bottom-up approach to prevent leader abusive supervision. [ABSTRACT FROM AUTHOR]

We have studied the polarized image of an equatorial emitting ring around a rotating black hole surrounded by a cold dark matter (CDM) halo. Results show that the CDM halo density has the similar effects of the halo’s characteristic radius on the polarized image for the black hole. The effects of the CDM halo on the polarized image depend on the magnetic field configuration, the fluid velocity and the observed inclination. With the increase of the CDM halo parameters, the observed polarization intensity decreases when the magnetic field lies in equatorial plane, but in the case where the magnetic field is perpendicular to the equatorial plane, the change of the observed polarization intensity with CDM halo also depends on the position of the emitting point in the ring. The change of the electric vector position angle (EVPA) with the CDM halo becomes more complicated. Our results also show that the influence of the CDM halo on the polarized image is generally small, which are consistent with the effects of dark matter halo on black hole shadows. These results could help to further understand dark matter from black hole images. [ABSTRACT FROM AUTHOR]

We have studied the polarized image of an equatorial emitting ring around a 4D Gauss–Bonnet black hole. Our results show that the effects of Gauss–Bonnet parameter on the polarized image depend on the magnetic field configuration, the observation inclination angle, and the fluid velocity. As the magnetic field lies in the equatorial plane, the observed polarization intensity increases monotonously with Gauss–Bonnet parameter in the low inclination angle case, and its monotonicity disappears in the case with high inclination angle. However, as the magnetic field is vertical to the equatorial plane, the polarization intensity is a monotonously increasing function of Gauss–Bonnet parameter in the high inclination angle case. The changes of the electric vector position angle with Gauss–Bonnet parameter in both cases are more complicated. We also probe the effects of Gauss–Bonnet parameter on the Strokes Q–U loops. [ABSTRACT FROM AUTHOR]

Malaria parasites are less vulnerable to mosquito immune responses once ookinetes transform into oocysts, facilitating parasite development in the mosquito. However, the underlying mechanisms of oocyst resistance to mosquito defenses remain unclear. Here, we show that circumsporozoite protein (CSP) is required for rodent malaria oocysts to avoid mosquito defenses. Mosquito infection with CSPmut parasites (mutation in the CSP pexel I/II domains) induces nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 5 (NOX5)-mediated hemocyte nitration, thus activating Toll pathway and melanization of mature oocysts, upregulating hemocyte TEP1 expression, and causing defects in the release of sporozoites from oocysts. The pre-infection of mosquitoes with the CSPmut parasites reduces the burden of infection when re-challenged with CSPwt parasites by inducing hemocyte nitration. Thus, we demonstrate why oocysts are invisible to mosquito immunity and reveal an unknown role of CSP in the immune evasion of oocysts, indicating it as a potential target to block malaria transmission. Circumsporozoite protein (CSP), the major surface protein of Plasmodium sporozoites, is important for parasite targeting to mosquito salivary glands and the mammalian liver. Here, Zhu et al. show that CSP is required for rodent malaria oocysts to evade mosquito immunity by inducing hemocyte nitration and causing subsequent defects in sporozoite-release from oocysts. [ABSTRACT FROM AUTHOR]

Cloud condensation nuclei (CCN) activities and hygroscopic properties of aerosol particles were studied based on the core-shell model with extended Köhler equation. Three kinds of core-shell particles were mainly considered: (1) insoluble core and soluble inorganic shell; (2) insoluble core and organic shell with limited solubility; (3) organic core with limited solubility and soluble inorganic shell. Results of the model calculations showed that Köhler curves of aerosol particles were different from classical Köhler curves of inorganic particles. Particle size, chemical composition and mass fraction of components all influence the curve shape, and the curve might show one or more extreme points of supersaturation. Critical supersaturation decreased when the mass fraction of the shell increased. This implied that the heterogeneous chemical reaction that happened to suspended particles in air could increase shell mass fraction and make particles more CCN active and hygroscopic. When the three models were compared, particles in model 3 were most CCN active, while those in model 2 were least CCN active. If the shell mass fraction was 0.1–0.2 for model 3 and 0.3–0.4 for model 1, the calculated hygroscopic parameter κ was closer to historical measuring results of 0.2–0.3 in polluted areas in China while the hygroscopic parameter would never be close to measuring results in model 2 no matter how high the shell mass fraction was. Based on discussion of two component particles of the core-shell model, more components in core-shell model could be considered, and the calculations were discussed in this article. [ABSTRACT FROM AUTHOR]

The activation of microglia is an important cause of central nervous system (CNS) inflammatory cell infiltration and inflammatory demyelination in experimental autoimmune encephalomyelitis (EAE). Furthermore, the proinflammatory response induced by the NLR family pyrin domain containing 3 (NLRP3) inflammasome can be amplified in microglia after NLRP3 inflammasome activation. Autophagy is closely related to the inflammatory response. Caffeine exerts anti-inflammatory and autophagy-stimulating effects, but the specific mechanism remains unclear. This study examined the mechanism underlying the anti-inflammatory effect of caffeine on EAE. In this study, C57BL/6 mice were immunized to induce EAE and treated with caffeine to observe its effect on prognosis. The effects of caffeine on autophagy and inflammation were also analysed in mouse primary microglia (PM) and the BV2 cell line. The data demonstrated that caffeine reduced the clinical score, the infiltration of inflammatory cells, the demyelination level, and the activation of microglia in EAE mice. Furthermore, caffeine increased the LC3-II/LC3-I levels and decreased the NLRP3 and P62 levels in EAE mice, whereas the autophagy inhibitor 3-methylamine (3-MA) blocked these effects. In vitro, caffeine promoted autophagy by suppressing the mechanistic target of rapamycin (mTOR) pathway and inhibited activation of the NLRP3 inflammasome. However, autophagy-related gene 5 (ATG5)-specific siRNA abolished the anti-inflammatory effect of caffeine treatment in PM and BV2 cells. Taken together, these data suggest that caffeine exerts a newly discovered effect on EAE by reducing NLRP3 inflammasome activation via the induction of autophagy in microglia. [ABSTRACT FROM AUTHOR]

Since the discovery of ketamine anti-depressant effects in last decade, it has effectively revitalized interest in investigating excitatory synapses hypothesis in the pathogenesis of depression. In the present study, we aimed to reveal the excitatory synaptic regulation of corticotropin-releasing hormone (CRH) neuron in the hypothalamus, which is the driving force in hypothalamic–pituitary–adrenal (HPA) axis regulation. This study constitutes the first observation of an increased density of PSD-93-CRH co-localized neurons in the hypothalamic paraventricular nucleus (PVN) of patients with major depression. PSD-93 overexpression in CRH neurons in the PVN induced depression-like behaviors in mice, accompanied by increased serum corticosterone level. PSD-93 knockdown relieved the depression-like phenotypes in a lipopolysaccharide (LPS)-induced depression model. Electrophysiological data showed that PSD-93 overexpression increased CRH neurons synaptic activity, while PSD-93 knockdown decreased CRH neurons synaptic activity. Furthermore, we found that LPS induced increased the release of glutamate from microglia to CRH neurons resulted in depression-like behaviors using fiber photometry recordings. Together, these results show that PSD-93 is involved in the pathogenesis of depression via increasing the synaptic activity of CRH neurons in the PVN, leading to the hyperactivity of the HPA axis that underlies depression-like behaviors. [ABSTRACT FROM AUTHOR]

Witnessing abusive supervision is pervasive in the workplace. Intuitively, observers should respond with empathy. Drawing on appraisal theory of emotion, however, we propose that observers' emotional and behavioral responses to witnessing abusive supervision depend on the perceived goal competitiveness between observers and victims. Specifically, when perceived goal competitiveness is high or made salient, observed abusive supervision is positively associated with observers' schadenfreude, which in turn decreases their helping behaviors toward victims. In contrast, when perceived goal competitiveness is low, observed abusive supervision is positively associated with observers' empathic emotion, which in turn increases their helping behaviors toward victims. Data from one experiment and one multi-wave field study provide support for these hypotheses. We discuss the theoretical and practical implications of these findings and directions for future research. [ABSTRACT FROM AUTHOR]

The strength of sandstone decreases significantly with higher water content attributing to softening effects. This scenario can pose a severe threat to the stability of reservoirs of pumped storage power stations developed from abandoned mines, especially when subjected to the cyclic loading condition caused by the repeated drainage and storage of water (fatigue damage). Based on this, it is essential to focus on the fatigue failure characteristics. In this study, the mineral composition of the used sandstone of Ruineng coal mine in Shanxi Province, China, was first tested to elucidate the rock softening mechanism after absorbing water. Next, a numerical model for replicating the mechanical behavior of water-bearing sandstone was established using two-dimensional particle flow code (PFC2D) with a novel contact model. Then, 16 uniaxial cyclic loading simulations with distinct loading parameters related to reservoir conditions (loading frequency, amplitude level, and maximum stress level) and different water contents were conducted. The numerical results show that all these three loading parameters affect the failure characteristics of sandstone, including irreversible strain, damage evolution, strain behavior, and fatigue life. The influence degree of these three parameters on failure behavior increases in the order of maximum stress level, loading frequency, and amplitude level. However, for the samples with different water contents, their failure characteristics are similar under the same loading conditions. Furthermore, the failure mode is almost unaffected by the loading parameters, while the water content plays a significant role and causing the transformation from the tensile splitting with low water content to the shear failure with higher water content. [ABSTRACT FROM AUTHOR]

We have studied polarized image of a Schwarzschild black hole with an equatorial thin accretion disk as photon couples to Weyl tensor. The birefringence of photon originating from the coupling affect the black hole shadow, the thin disk pattern and its luminosity distribution. We also analyze the observed polarized intensity in the sky plane. The observed polarized intensity in the bright region is stronger than that in the darker region. The stronger effect of the coupling on the observed polarized vector appears only in the bright region close to black hole. These features in the polarized image could help us to understand black hole shadow, the thin accretion disk and the coupling between photon and Weyl tensor. [ABSTRACT FROM AUTHOR]

Modern cyber-physical systems (CPS) and the Internet of things (IoT) are data factories generating, measuring and recording huge amounts of time series. The useful information in time series is usually present in the form of sequential patterns. We propose shape expressions as a declarative language for specification and extraction of rich temporal patterns from possibly noisy data. Shape expressions are regular expressions with arbitrary (linear, exponential, sinusoidal, etc.) shapes with parameters as atomic predicates and additional constraints on these parameters. We associate with shape expressions novel noisy semantics that combines regular expression matching semantics with statistical regression. We study essential properties of the language and propose an efficient heuristic for approximate matching of shape expressions. We demonstrate the applicability of this technique on two case studies from the health and the avionics domains. [ABSTRACT FROM AUTHOR]

Various studies have recognized the importance of humility as a foundational aspect of virtuous leadership and have revealed the beneficial effects of leader humility on employee moral attitudes and behaviors. However, these findings may overestimate the benefits of leader humility and overlook its potential costs. Integrating person–supervisor fit theory and balance theory with the humility literature, we employ a dyadic approach to consider supervisor and employee humility simultaneously. We investigate whether and how the (in)congruence of supervisor and employee humility influences employee citizenship and deviance behaviors. We conducted a multilevel, multiphase, and multisource field study to test our hypotheses. The results of cross-level polynomial regression analyses revealed that when supervisors and employees were incongruent in humility, employees experienced higher levels of negative affect toward supervisors. Also, compared to those in low–low congruent dyads, employee negative affect toward supervisors was lower in high–high congruent dyads. The results further revealed asymmetric incongruence effects: employees experienced the highest levels of negative affect toward supervisors when their own humility was lower than their supervisors'. In addition, we found that employee negative affect toward supervisors mediated the impacts of supervisor–employee (in)congruence in humility on employee organizational citizenship behavior and counterproductive work behavior. [ABSTRACT FROM AUTHOR]

Chronic adenosine A1R stimulation in hypoxia leads to persistent hippocampal synaptic depression, while unopposed adenosine A2AR receptor stimulation during hypoxia/reperfusion triggers adenosine-induced post-hypoxia synaptic potentiation (APSP) and increased neuronal death. Still, the mechanisms responsible for this adenosine-mediated neuronal damage following hypoxia need to be fully elucidated. We tested the hypothesis that A1R and A2AR regulation by protein kinase casein kinase 2 (CK2) and clathrin-dependent endocytosis of AMPARs both contribute to APSPs and neuronal damage. The APSPs following a 20-min hypoxia recorded from CA1 layer of rat hippocampal slices were abolished by A1R and A2AR antagonists and by broad-spectrum AMPAR antagonists. The inhibitor of GluA2 clathrin-mediated endocytosis Tat-GluA2-3Y peptide and the dynamin-dependent endocytosis inhibitor dynasore both significantly inhibited APSPs. The CK2 antagonist DRB also inhibited APSPs and, like hypoxic treatment, caused opposite regulation of A1R and A2AR surface expression. APSPs were abolished when calcium-permeable AMPAR (CP-AMPAR) antagonist (IEM or philanthotoxin) or non-competitive AMPAR antagonist perampanel was applied 5 min after hypoxia. In contrast, perampanel, but not CP-AMPAR antagonists, abolished APSPs when applied during hypoxia/reperfusion. To test for neuronal viability after hypoxia, propidium iodide staining revealed significant neuroprotection of hippocampal CA1 pyramidal neurons when pretreated with Tat-GluA2-3Y peptide, CK2 inhibitors, dynamin inhibitor, CP-AMPAR antagonists (applied 5 min after hypoxia), and perampanel (either at 5 min hypoxia onset or during APSP). These results suggest that the A1R-CK2-A2AR signaling pathway in hypoxia/reperfusion injury model mediates increased hippocampal synaptic transmission and neuronal damage via calcium-permeable AMPARs that can be targeted by perampanel for neuroprotective stroke therapy. [ABSTRACT FROM AUTHOR]

Purpose: To study neurochemical reactions to chronic intermittent hypoxia (CIH) in the hypoglossal nucleus (HN) of rats. Methods: Adult male Sprague-Dawley rats (n = 12) were randomly divided into two groups (the CIH and the control group). The CIH rats were housed in a hypoxic chamber with the fraction of oxygen volume alternating between 21% and 5% by providing air for 60 s and then providing nitrogen for 60 s from 8:30 am to 16:30 pm each day for 35 days. The control group was housed in a cabin with normal oxygen levels. We studied the expression of c-fos protein, 5-hydroxytryptamine (5-HT) positive terminals, and its 2A receptors in hypoglossal nuclei by immunohistochemistry. Results: The expression of c-fos, 5-HT positive terminals, and accordingly 5-HT 2A receptors in the CIH group were significantly higher than that in the controls (p < 0.05). The ventral side of the HN showed a clearly higher expression of 5-HT and its 2A receptors than the dorsal side (p < 0.05). Conclusion: There were 2 responses of the HN to CIH. First, CIH induced a higher expression of 5-HT positive terminals and its 2A receptors, and second, this reaction was much more evident in ventral side than in the dorsal side. We postulate that these responses may serve to be a protective and compensatory mechanism for CIH. [ABSTRACT FROM AUTHOR]

The ventral part of the anteromedial thalamic nucleus (AMv) is in a position to convey information to the cortico-hippocampal-amygdalar circuit involved in the processing of fear memory. Corticotropin-releasing-factor (CRF) neurons are closely associated with the regulation of stress and fear. However, few studies have focused on the role of thalamic CRF neurons in fear memory. In the present study, using a conditioned fear paradigm in CRF transgenic mice, we found that the c-Fos protein in the AMv CRF neurons was significantly increased after cued fear expression. Chemogenetic activation of AMv CRF neurons enhanced cued fear expression, whereas inhibition had the opposite effect on the cued fear response. Moreover, chemogenetic manipulation of AMv CRF neurons did not affect fear acquisition or contextual fear expression. In addition, anterograde tracing of projections revealed that AMv CRF neurons project to wide areas of the cerebral cortex and the limbic system. These results uncover a critical role of AMv CRF neurons in the regulation of conditioned fear memory. [ABSTRACT FROM AUTHOR]

Contributing to abusive supervision, creative leadership, and negative creativity research, we examine how and when leaders' creative mindset relates to interpersonal aggression toward followers in the form of abusive supervision. Drawing upon moral disengagement theory, we theorize that leaders' daily creative mindset positively relates to daily episodes of abusive supervision via state-based moral disengagement. Furthermore, we propose that trait-based moral disengagement moderates this indirect process such that low trait-based moral disengagement diminishes this effect. We found support for our hypotheses using a longitudinal study with a daily data collection over a 2-week period (Study 1) and an experimental study (Study 2). Our findings reveal the potential perils of leader creativity in leader-follower contexts and the importance of considering the moral disengagement process. [ABSTRACT FROM AUTHOR]

Fracturing surface micro-seismic monitoring technology is widely used in fracturing surface. Micro- seismic monitoring conducted by means of the internet is composed of a large number of geophones laid on the ground to collect subsurface micro seismic signals and monitor fracturing fractures. With the advantages of a large amount of monitoring data, flexible layout, easy adjustment, and low cost. The technology also has some disadvantages, such as weak signal reception, vulnerable to environmental impact and low signal-to-noise ratio. In order to improve the positioning accuracy and receive as many effective signals as possible, a signal receiving network model of optimal geophone for micro-seismic monitoring on a fractured surface based on improved genetic algorithm is proposed. Through simulation and numerical analysis, the solution model has been optimized to meet the accuracy and quickly solve the optimal geophone array scheme. The results show that the optimal geophone array scheme not only satisfies the positioning accuracy but also achieves better reception of micro-seismic signals as possible with a small number of geophones, which reduces the cost and promotes the industrial application of this technology. [ABSTRACT FROM AUTHOR]

The environmental fate and behavior of arsenic (As) is receiving increased attention due to the As pollution all over the world. Kaolinite is a natural mineral resource that can be used for the removal of pollution as adsorbent. First-principles calculations based on density-functional theory was employed to explore the adsorption behavior of arsenic atoms adsorption on the kaolinite (001) and (00 1 ¯ ) surface. The one-fold top site was found to be energetically preferred for the kaolinite (001) and (00 1 ¯ ) surface, and the adsorption energy of the former is obviously higher than the latter. The coverage dependence of the adsorption energetics was systematically studied for a wide range of coverage Θ [from 0.11 to 1.0 monolayers (ML)]. The adsorption energy of As decreased with increasing coverage, thus indicating the lower stability of surface adsorption due to the repulsion of neighboring As atoms. The coverage has obvious effects on the arsenic adsorption process. Other properties of the As/kaolinite (001) system, including the lattice relaxation and changes of electronic density of states, were also studied and discussed in detail. [ABSTRACT FROM AUTHOR]

Deep unsupervised domain adaptation has recently received increasing attention from researchers. However, existing methods are computationally intensive due to the computational cost of convolutional neural networks (CNN) adopted by most work. There is no effective network compression method for such problem. In this paper, we propose a unified transfer channel pruning (TCP) method for accelerating deep unsupervised domain adaptation (UDA) models. TCP method is capable of compressing the deep UDA model by pruning less important channels while simultaneously learning transferable features by reducing the cross-domain distribution divergence. Therefore, it reduces the impact of negative transfer and maintains competitive performance on the target task. To the best of our knowledge, TCP method is the first approach that aims at accelerating deep unsupervised domain adaptation models. TCP method is validated on two main kinds of UDA methods: the discrepancy-based methods and the adversarial-based methods. In addition, it is validated on two benchmark datasets: Office-31 and ImageCLEF-DA with two common backbone networks - VGG16 and ResNet50. Experimental results demonstrate that our TCP method achieves comparable or better classification accuracy than other comparison methods while significantly reducing the computational cost. To be more specific, in VGG16, we get even higher accuracy after pruning 26% floating point operations (FLOPs); in ResNet50, we also get higher accuracy on half of the tasks after pruning 12% FLOPs for both discrepancy-based methods and adversarial-based methods. [ABSTRACT FROM AUTHOR]

Given the rapid development in precision medicine, tremendous efforts have been devoted to discovering new biomarkers for disease diagnosis and treatment. Esophageal cancer-related gene-4 (ECRG4), which is initially known as a new candidate tumor suppressor gene, is emerging as a sentinel molecule for gauging tissue homeostasis. ECRG4 is unique in its cytokine-like functional pattern and epigenetically-regulated gene expression pattern. The gene can be released from the cell membrane upon activation and detected in liquid biopsy, thus offering considerable potential in precision medicine. This review provides an updated summary on the biology of ECRG4, with emphasis on its important roles in cancer diagnosis and therapy. The future perspectives of ECRG4 as a potential molecular marker in precision medicine are also discussed in detail. [ABSTRACT FROM AUTHOR]

Increasing evidence demonstrates that pyroptosis, pro-inflammatory programmed cell death, is linked to acute lung injury (ALI). Dihydromyricetin (DHM) has been reported to exert anti-inflammatory effects by inhibiting NLRP3 inflammasome activation in vascular endothelial cells. However, the effects of DHM on NLRP3 inflammasome-induced pyroptosis in ALI remain elusive. In the present study, male BALB/c mice were subjected to cecal ligation and puncture (CLP), and DHM (50, 100, 150 mg/kg) was orally administered (once per day, for 3 days) 2 h after CLP. After 72 h, lung histopathology was examined, and the wet/dry (W/D) ratio, inflammatory infiltration, total protein concentration, total cell, and neutrophil counts were detected. Myeloperoxidase (MPO), interleukin (IL)-6, TNF-α, IL-1β, and IL-18 levels in bronchoalveolar lavage fluid (BALF) were measured by ELISA. Additionally, the expression of NLRP3 signaling pathway proteins were detected by Western blotting. The results revealed that in BALF, DHM (150 mg/kg) treatment significantly reduced the CLP-induced lung histopathological injury, inflammatory cell infiltration, total cell and neutrophil number, and total protein and albumin concentration. DHM treatment significantly inhibited the CLP-induced NLRP3 inflammasome pathway (NLRP3, ASC, caspase-1, gasdermin D (Gsdmd), IL-1β, and IL-18). In conclusion, these results demonstrate that DHM protects against CLP-induced ALI by inhibiting NLRP3 inflammasome activation and subsequent pyroptosis. [ABSTRACT FROM AUTHOR]

Recent research has uncovered the dark side of creativity by finding that creative individuals are more likely to engage in unethical behavior. However, we argue that not all creative individuals make trouble. Using moral self-regulation theory as our overarching theoretical framework, we examine individuals' moral identity as a boundary condition and moral disengagement as a mediating mechanism to explain when and how individual creativity is associated with workplace deviant behavior. We conducted two field studies using multi-source data to test our hypotheses. In Study 1, the results indicated that creativity positively predicted moral disengagement for those low in moral identity. In Study 2 with multi-wave data, we replicated the finding that moral identity moderated the effect of creativity on moral disengagement in Study 1 and further revealed that moral disengagement mediated the interactive effects of creativity and moral identity on workplace deviant behavior. The theoretical and practical implications of these findings and directions for future research are discussed. [ABSTRACT FROM AUTHOR]

Multiple sclerosis (MS) is a chronic, autoimmune, inflammatory, and demyelinating disorder of the central nervous system (CNS), which ultimately leads to axonal loss and permanent neurological disability. Current treatments for MS are largely comprised of medications that are either immunomodulatory or immunosuppressive and are aimed at reducing the frequency and intensity of relapses. Neural stem cells (NSCs) in the adult brain can differentiate into oligodendrocytes in a context-specific manner and are shown to be involved in the remyelination in these patients. NSCs may exert their beneficial effects not only through oligodendrocyte replacement but also by providing trophic support and immunomodulation, a phenomenon now known as “therapeutic plasticity.” In this review, we first provided an update on the current knowledge regarding MS pathogenesis and the role of immune cells, microglia, and oligodendrocytes in MS disease progression. Next, we reviewed the current progress on research aimed toward stimulating endogenous NSC proliferation and differentiation to oligodendrocytes in vivo and in animal models of demyelination. In addition, we explored the neuroprotective and immunomodulatory effects of transplanted exogenous NSCs on T cell activation, microglial activation, and endogenous remyelination and their effects on the pathological process and prognosis in animal models of MS. Finally, we examined various protocols to generate genetically engineered NSCs as a potential therapy for MS. Overall, this review highlights the studies involving the immunomodulatory, neurotrophic, and regenerative effects of NSCs and novel methods aiming at stimulating the potential of NSCs for the treatment of MS. [ABSTRACT FROM AUTHOR]

Protozoan parasites can synthesize polyunsaturated fatty acids. They possess stearoyl-CoA desaturase to convert stearate into oleate and linoleate. Stearoyl-CoA desaturase are the key enzymes required for the synthesis of unsaturated fatty acids. It seems attractive to evaluate the possibility of using unsaturated fatty acid biosynthesis pathways as drug targets. In this study, the authors investigate codon usage bias, base composition variations and protein sequence in ten available complete stearoyl-CoA desaturase gene sequences from Toxoplasma gondii, Neospora caninum etc. The results show that fatty acid desaturase genes GC content high of parasitic protozoa genes, GC content up to 63.37%, while fatty acid desaturase genes of parasitic protozoa prefers to use codon ending with G/C. In addition, the expected curve was also drawn to reveal the relationship of ENC and GC3s when the codon usage was only subjected to the nucleotide composition constraint. The genes lied on the expected curve in ENC-plot, indicating nucleotide composition constraint played a role in the condon usage pattern. Protein analysis, we find that all proteins are stearoyl-CoA desaturase, have sites of iron-binding active centers and contain three conserved His-rich motifs. If stearoyl-CoA desaturase is unusual to these parasites, it provides basis as a promising target for the development of selective chemical intervention. Therefore, the Bioinformatics analysis of protein and codon can help improve the work of genetic engineering and drug screening. [ABSTRACT FROM AUTHOR]

Cellulose nanopaper exhibits outstanding stiffness, strength, and toughness that originate from the exceptional properties of constituent cellulose nanocrystals (CNCs). However, it remains challenging to link the nanoscale properties of rod-like CNCs and their structural arrangements to the macroscale performance of nanopaper in a predictive manner. Here we address this need by establishing an atomistically informed coarse-grained model for CNCs via a strain energy conservation paradigm and simulating CNC nanopaper properties mesoscopically. We predict how the mechanical properties of CNC nanopaper with nacre-inspired brick-and-mortar structure depend on CNC overlap length and interfacial energy. We show that the modulus and strength both increase with increasing overlap length, but saturate at different critical length scales where a transition from non-covalent interfacial sliding to CNCs fracture is the key influencing mechanism. Maximum toughness is achieved when the interface and CNC failure are tuned to occur at the same time through balanced failure. We propose strategies for maximizing nanopaper mechanical performance by tuning interfacial interactions of constitutive CNCs through surface modifications that improve shear transfer capability. Our model generates broadly applicable insights into factors governing the performance of self-assembling paper materials made from 1D nanostructures. [ABSTRACT FROM AUTHOR]

Metal nanoparticles have been combined with magnet metal-organic frameworks (MOFs) to afford new materials that demonstrate an efficient catalytic degradation, high stability, and excellent reusability in areas of catalysis because of their exceptionally high surface areas and structural diversity. Magnetic MO@N-C (M = Fe, Co, Mn) nanocrystals were formed on nitrogen-doped carbon surface by using 8-hydroxyquinoline as a C/N precursor. The Co@N-C, MnO@N-C, and Fe/FeO@N-C catalysts were characterized by X-ray diffraction (XRD), Raman, scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), N adsorption/desorption, and X-ray photoelectron spectroscopy (XPS). The catalytic performances of catalysts were thoroughly investigated in the oxidation of aniline solution based on sulfate radicals (SO ) toward Fenton-like reaction. Magnetic MO@N-C exhibits an unexpectedly high catalytic activity in the degradation of aniline in water. A high magnetic MO@N-C catalytic activity was observed after the evaluation by aniline degradation in water. Aniline degradation was found to follow the first-order kinetics, and as a result, various metals significantly affected the structures and performances of the catalysts, and their catalytic activity followed the order of Co > Mn > Fe. The nanoparticles displayed good magnetic separation under the magnetic field. [ABSTRACT FROM AUTHOR]

In this study, we elucidated the microbial transglutaminase-induced gelation properties and thermal gelling ability of soy protein isolate (SPI) and wheat gluten (WG) mixture following ultrahigh pressure (UHP, 100-400 MPa) pretreatment. UHP treatment induced unfolding and aggregation within SPI/WG protein molecules, which led to increases in free sulfhydryl group content and surface hydrophobicity. However, the transglutaminase cross-linking reaction facilitated the formation of hydrophobic interactions and disulfide bonds and thus resulted in higher gel strength, water holding capacity, and denser and more homogeneous gel networks of transglutaminase cross-linked SPI/WG gels. Rheological measurements revealed that the addition of UHP steps might generate a higher storage modulus ( G′) value of MTGase-induced SPI/WG gelation during the heating-cooling cycle (25 °C → 95 °C → 25 °C). Our results indicated that various chemical interactions including covalent interactions (i.e., ε-(γ-glutamyl)lysine bonds and disulfide bonds) and non-covalent interactions (i.e., electrostatic forces and hydrophobic interactions) were involved in SPI/WG gel network structures. Hydrophobic interactions and disulfide bonds are significantly increased with the pressure level (100-400 MPa) compared with that of the unpressurized control. Furthermore, UHP treatment reduced the α-helix and β-turn content but increased the β-sheet and random coil structures. Thus, UHP treatment may be considered as a novel technique to expand the utilization of SPI/WG mixture in the food protein gelation industry. [ABSTRACT FROM AUTHOR]

Minocycline, a semi-synthetic second-generation derivative of tetracycline, has been reported to exert neuroprotective effects both in animal models and in clinic trials of neurological diseases. In the present study, we first investigated the protective effects of minocycline on oxygen-glucose deprivation and reoxygenation-induced impairment of neurite outgrowth and its potential mechanism in the neuronal cell line, PC12 cells. We found that minocycline significantly increased cell viability, promoted neurite outgrowth and enhanced the expression of growth-associated protein-43 (GAP-43) in PC12 cells exposed to oxygen-glucose deprivation/reoxygenation injury. In addition, immunoblots revealed that minocycline reversed the overexpression of phosphorylated myosin light chain (MLC) and the suppression of activated extracellular signal-regulated kinase 1/2 (ERK1/2) caused by oxygen-glucose deprivation/reoxygenation injury. Moreover, the minocycline-induced neurite outgrowth was significantly blocked by Calyculin A (1 nM), an inhibitor of myosin light chain phosphatase (MLCP), but not by an ERK1/2 inhibitor (U0126; 10 μM). These findings suggested that minocycline activated the MLCP/MLC signaling pathway in PC12 cells after oxygen-glucose deprivation/reoxygenation injury, which resulted in the promotion of neurite outgrowth. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome of Ampittia dioscorides (Lepidoptera: Hesperiidae) was determined. The sequenced genome is a circular molecule of 15313 bp, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and an A + T-rich region. The gene arrangements and transcribing directions are identical to those in most of the reported lepidopteran mitogenomes. The base composition of the whole genome and genes or regions are also similar to those in other lepidopteran species. All the PCGs are initiated by typical ATN codons; the exception being COI, which begins with a CGA codon. Eight genes (ND2, ATPase8, ATPase6, COIII, ND5, ND4L, ND6, and Cytb) end with a TAA stop codon, and two genes (ND1 and ND3) end with TAG. The remaining three genes ( COI and COII, which end with TA-, and ND4, which ends with T-) have incomplete stop codons. All tRNAs have the typical clover-leaf structure of mitochondrial tRNAs, with the exception of tRNA(AGY). On the basis of the concatenated nucleotide and amino acid sequences of the 13 PCGs and wingless gene of 22 butterfly species, maximum parsimony (MP) and Bayesian inference (BI) trees were constructed, respectively. Both MP and BI trees had the same topological structure: ((((Nymphalidae + Danaidae) + Lycaenidae) + Pieridae) + Papilionidae) + Hesperiidae). The results provide support for Hesperiidae as a superfamily-level taxon. [ABSTRACT FROM AUTHOR]

Current knowledge of RNA virus biodiversity is both biased and fragmentary, reflecting a focus on culturable or disease-causing agents. Here we profile the transcriptomes of over 220 invertebrate species sampled across nine animal phyla and report the discovery of 1,445 RNA viruses, including some that are sufficiently divergent to comprise new families. The identified viruses fill major gaps in the RNA virus phylogeny and reveal an evolutionary history that is characterized by both host switching and co-divergence. The invertebrate virome also reveals remarkable genomic flexibility that includes frequent recombination, lateral gene transfer among viruses and hosts, gene gain and loss, and complex genomic rearrangements. Together, these data present a view of the RNA virosphere that is more phylogenetically and genomically diverse than that depicted in current classification schemes and provide a more solid foundation for studies in virus ecology and evolution. [ABSTRACT FROM AUTHOR]

We have previously demonstrated the radio-sensitizing effect of zoledronic acid (ZOL), a third generation bisphosphonate, on human esophageal squamous cell carcinoma (ESCC) cells. Here we show that ZOL suppresses metastatic progression of ESCC cells mainly through up-regulating the tight junction protein occludin. Exposure to ZOL at lower concentrations dramatically reduced migration and invasion of ESCC cells. In addition, ZOL treatment decreased the expression of mesenchymal markers, vimentin and N-cadherin, while increased the expression of the tight junction protein occludin. Moreover, ectopic expression of Slug, a well-known transcriptional repressor of occludin, partially but significantly abrogated the effect of ZOL on occludin expression and subsequently rescued the malignant metastatic phenotype, suggesting that Slug is one of the mediators underlying the anti-metastatic effect of ZOL. The present study is the first to report the significance of ZOL on ESCC metastasis. These data are promising for the future application of this drug regimen in patients with ESCC. [ABSTRACT FROM AUTHOR]

This study focuses on the conditions under which emotional exhaustion leads to employee unsafe behavior. In a sample of 592 construction workers nested in 33 groups, we found that both emotional exhaustion and unsafe behavior norms were positively related to unsafe behavior by employees. Unsafe behavior norms moderated the relationship between emotional exhaustion and unsafe behavior, such that high group unsafe behavior norms strengthened the emotional exhaustion-employee unsafe behavior link. Furthermore, results indicated a three-way interaction effect in which employees with high emotional exhaustion conducted the highest levels of unsafe behavior when both group unsafe behavior norms and personal control over work were high. This paper provides important implications on understanding the influence of group norms on employee unsafe behavior, as well as its magnifying effect with personal control on the emotional exhaustion-unsafe behavior link. [ABSTRACT FROM AUTHOR]

Background: Recently, we identified the esophageal carcinoma related gene 4 ( ECRG4) as a novel candidate tumor suppressor gene and a promising therapeutic target in nasopharyngeal carcinoma (NPC). In addition, we found that reduced ECRG4 expression in NPC was associated with promoter hypermethylation. The aim of the current study was to assess the expression status of the ECRG4 protein in breast cancer and to clarify its clinicopathological significance and potential prognostic implications. Methods: Western blotting was used to examine ECRG4 protein levels in 20 paired breast cancer tissues and adjacent noncancerous tissues. In addition, we performed ECRG4 immunohistochemistry on 113 clinicopathologically well-characterized breast cancer samples and assessed putative associations between its expression and overall patient survival rates. Results: We found that ECRG4 protein expression was significantly reduced in the breast cancer tissues compared to the noncancerous tissues. Clinicopathological analyses revealed that loss of ECRG4 protein expression, observed in 41.6 % (47/113) of the primary breast cancer tissues tested, was significantly correlated with lymph node metastasis ( P = 0.026), advanced tumor stage ( P = 0.042) and unfavorable overall survival ( P = 0.004). Additional multivariate analyses revealed that ECRG4 protein expression may serve as an independent prognostic factor for the prediction of patient survival ( P = 0.033). Conclusion: Our data suggest that loss of ECRG4 protein expression may be involved in tumor progression and may serve as a prognostic biomarker for breast cancer. [ABSTRACT FROM AUTHOR]

Background: Cyclin-dependent kinase 10 (CDK10) has recently been identified as a tumor suppressor and, concordantly, its encoding gene has frequently been found to be inactivated in various human cancers. Here, we examined the expression status of CDK10 in a panel of primary human breast cancers and evaluated its correlation with clinicopathological parameters and clinical outcome. Methods: Western blotting was used to assess CDK10 protein levels in 20 paired breast cancer tissues and adjacent noncancerous tissues. In addition, immunohistochemistry was performed in 128 formalin-fixed, paraffin-embedded tumor tissues. Associations of CDK10 expression with various clinicopathological parameters were evaluated and Kaplan-Meier survival analyses and Cox proportional hazards models were used to estimate its effect on patient survival. Results: We found that CDK10 protein expression was markedly decreased in cancer tissues compared to adjacent noncancerous tissues. Immunohistochemistry revealed decreased CDK10 levels in 65/128 (50.8 %) of the primary breast cancer tissues tested. These decreased levels were found to be significantly associated with lymph node metastasis ( P = 0.003), advanced tumor stage ( P < 0.001) and unfavorable overall survival ( P < 0.001). Furthermore, multivariate analyses indicated that CDK10 expression may serve as an independent prognostic factor for survival ( P = 0.001). Conclusion: Down-regulated CDK10 expression frequently occurs in breast cancers and correlates with disease progression and poor survival. CDK10 may serve as a prognostic biomarker for breast cancer. [ABSTRACT FROM AUTHOR]

The complete mitochondrial genome sequence of Lamproptera curia was determined in the present study. Our findings showed that the mtDNA of L. curia had a typical organization of insect mitochrondrial DNA - being 15277 base pairs in length, it contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region (CR). The newly determined sequence was used for phylogenetic analyses, together with those of 45 species of Lepidoptera published elsewhere, including sequences of three species of Diptera as outgroups. The phylogenetic trees were constructed using the concatenated amino acid and nucleotide sequences of the 13 protein-coding genes (PCGs) based on the maximum likelihood (ML) and Bayesian inference (BI) methods. Both BI and ML trees revealed a similar topology structure: (((((Bombycoidea + Geometroidea) + Noctuoide) + Pyraloidea) + (Papilionoidea + Hesperioidea)) + Tortricoidea). Furthermore, the phylogenetic analyses demonstrated that each of the 16 families belonged to a monophyletic group respectively. The results of molecular phylogeny from the present study were congruent with traditional classification based on morphology but failed to demonstrate the monophyly of Hesperiidae. [ABSTRACT FROM AUTHOR]