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The study seeks to contribute novel insights into the efficacy of rhizobacteria, simultaneous Azospirillum brasilense and Bacillus subtilis inoculation as a means to not only mitigate the detrimental impacts of salt stress but also to potentially achieve superior results in terms of wheat biomass production. The experimental design involved a pot experiment where wheat plants were subjected to nine treatments {T0 = Control [No seed inoculation with any bacterial strain + Non-saline soil (2.16 dS/m)], T1 = Saline soil (6.0 dS/m), T2 = Saline soil + Azospirillum brasilense, T3 = Saline soil + Bacillus subtilis, T4 = Saline soil + A. brasilense + B. subtilis, T5 = Highly saline (10 dS/m), T6 = Highly saline + Azospirillum brasilense, T7 = Highly saline + Bacillus subtilis, T8 = Highly saline + A. brasilense + B. subtilis}. A. brasilense and B. subtilis individually exhibited positive effects in alleviating the detrimental influence of salt stress but combined application of both rhizobacteria showed superior effectiveness, particularly in saline and highly saline environments. A. brasilense and B. subtilis were found to enhance wheat plant growth by fostering improvements in photosynthesis, chlorophyll content, and crop growth rate, particularly in stressful conditions. Both rhizobacteria were improved biomass of wheat crop and other growth parameters. This study demonstrated the potential of A. brasilense and B. subtilis as beneficial rhizobacteria for enhancing wheat biomass production in the face of salt stress. Combined application of A. brasilense and B. subtilis could be a promising strategy for improving wheat growth under saline soils. [ABSTRACT FROM AUTHOR]

Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) is the most devastating insect-pest in cotton crop. It is vector of the cotton leaf curl virus (CLCV) and is responsible for huge losses to cotton industry in Pakistan and worldwide. It is mainly controlled by insecticides but the injudicious use of insecticides has resulted in insecticide resistance and population resurgence in addition to various harmful effects on the humans, non-target organisms and the environment. Transgenerational studies are very helpful to choose a best insecticidal option. In the current study, age-stage two-sex life table analysis was used to identify transgenerational effects of sublethal doses of afidopyropen. The adults of B. tabaci were treated with three concentrations of afidopyropen i.e., LC10, LC30 and LC50. The results indicated significant changes in the progeny i.e. the fecundity decreased in treated population; and female and male longevity of their progeny were more in control as compared to treated populations. Similarly, population parameters like intrinsic rate of growth (r), net reproductive rate (R0) and limiting rate of growth (λ) were significantly decreased in the treated adult progeny with values of 0.08–0.11, 4.85–7.46 and 1.09–1.12 per day, respectively. Based on the reduced biotic potential, afidopyropen can be suggested as an effective alternative option for the management of B. tabaci. [ABSTRACT FROM AUTHOR]

Cotton leaf curl disease (CLCuD) is a major constraint in the production of cotton crop across the Africa and Asia which is caused by a complex of begomoviruses (family Geminiviridae). Cotton leaf curl Khokhran virus and associated Cotton leaf curl Multan betasatellite (CLCuKoV/CLCuMB) complex are important agents of CLCuD which transmitted by whiteflies of the Bemisia tabaci species complex. Information regarding the transmission efficiency of CLCuKoV/CLCuMB by whitefly cryptic species is seriously lacking in Pakistan, therefore present study is conducted for comparison of transmission efficiency of CLCuKoV/CLCuMB by two characterized whitefly cryptic species (Asia II-1 and Asia II-5). Whitefly cryptic species were reared separately on Nicotiana benthamiana plants infected with CLCuKoV/CLCuMB for 24 or 48 h and these infected whitefly cryptic species were transferred to un-infected cotton and alternative host plants for 24 or 48 h. The first experiment results showed that Asia II-1 was able to transmit CLCuKoV/CLCuMB with high efficiency in cotton and bitter gourd plants as compared to the Asia II-5 while other host plants did not develop symptoms. Further in the second experiment results revealed that Asia II-1 was able to transmit CLCuKoV/CLCuMB with high efficiency from symptomatic bitter gourd to cotton plants as compared to Asia II-5 which did not show symptoms of transmission. Infection symptoms were confirmed by visual inspection after 30 days and PCR analysis. We found the percentage of virus infection symptoms in alternate host plants differed between two whitefly cryptic species due to varying transmission efficiencies. This research finding advances our understanding to explore the whitefly cryptic species involved in the transmission of CLCuD and will aid in the development of management strategies for control of whitefly cryptic species and CLCuD transmission in Pakistan. [ABSTRACT FROM AUTHOR]

Background: Bhendi or okra (Abelmoschus esculentus) is an important crop widely cultivated in the Indian subcontinent. The production of okra in tropical regions is constrained by several abiotic and biotic factors. Among biotic stresses, yellow vein mosaic disease (YVMD), transmitted by the whitefly (Bemisia tabaci Genn.), causes significant production losses. Results: Leaves showing symptoms of YVMD were collected from okra plants in the district of Mardan, Pakistan, from which the total nucleic acid was extracted. It was found that the viral genome was 2739 bp in the length and had seven conserved open reading frames, comparable to those of monopartite begomovirus species from the Old World. Nucleotide sequence comparison revealed that the genome has 97.7% identity with bhendi yellow vein mosaic virus (BYVMV). Additionally, alpha- and betasatellite DNA components were amplified and sequenced. The alpha- and betasatellite DNA sequences were 1367 and 1346 nt in length, respectively. Sequence analysis revealed that the alpha- and betasatellite sequences shared 97.9 and 98.7% similarity with cotton leaf curl Multan alphasatellite DNA (CLCuMuA) and croton yellow vein mosaic betasatellite DNA (CroYVMB), respectively. Conclusions: Based on the prevailing classification system, the isolate was identified as a variant of BYVMV, CLCuMuA, and CroYVB. Alphasatellite presence in the begomovirus betasatellite complex detected in the present study indicated a recent mobilization into the viral complex infecting okra in this region. The study findings may facilitate the design of new management strategies to protect this valuable crop against begomovirus infection. [ABSTRACT FROM AUTHOR]

Melatonin, a secretion from pineal gland is ambiguously considered as the key hormone involved in regulation of the antler cycle in Sika deer. To find out more about the roles of melatonin and its receptor gene, we carried out current study to investigate the association between polymorphisms in melatonin receptor 1A ( MTNR1A) gene and the antler yield from Sika deer. A total of 251 Sika deer were analyzed in this study, of which consisted of Wusan Sika deer ( n = 163) and Dongfeng Sika deer ( n = 88). MTNRA gene was amplified by PCR and genotyped by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Three polymorphism loci (C518T, C629G and C635T) were detected in exon2 of MTNR1A gene. The restriction site Ecol881 was used for C518T while a C629G polymorphism locus was digested with Mval restriction endonucleases. In Wusan Sika deer the allele frequencies of C and T were 0.637 and 0.363 for C518T, Also C and G alleles in C629G locus were 0.206 and 0.794. Genotypic frequencies of allele CC, CT and TT were 33.7, 59.9 and 6.4 % respectively, It showed 1.8, 37.4 and 60.7 % for frequencies of genotypes CC, CG and GG. In Dongfeng Sika deer the allele frequencies of C and T were 0.518 and 0.482 for C518T, C and G alleles were 0.375 and 0.625 for C629G. Genotypic frequencies were 10.6, 82.4 and 7.1 % for genotypes CC, CT and TT respectively, and they were 1.1, 72.7 and 26.2 % for genotypes CC, CG and GG. Among three SNPs, only C629G showed significant association ( P < 0.05) with average antler yield in Wusan Sika deer, while no SNP was significant in Dongfeng Sika deer. These preliminary results implied that the identified SNPs of MTNR1A gene might influence the antler yield in Wusan Sika deer. [ABSTRACT FROM AUTHOR]

Fasciolosis is an economically important disease for livestock, as well as being zoonotic. Recent figures on the prevalence of this disease have caused alarm concerning its potential for an increased prevalence in the future. The prevalence of fascioliosis has been documented from different regions of the world, helping us identify areas where future research needs to be focused. This manuscript is a review of the current status of the disease, the pathogenic species involved, diagnostic techniques (with new modifications and comparative specificity, sensitivity, and rapidity of these tests), chemotherapy, and vaccination. This also encompasses inaccurate reports on vaccination and drug development as well as the latest technologies to find promising candidates for drugs and vaccines. Drugs with lower efficacy have been used on some farms which lead to exacerbation of the clinical disease, presumably due to the development of drug resistance. Future studies should be focused on (1) the use of the most reliable diagnostic tests for periodic monitoring of the disease, (2) insights of the ecobiology and transmission dynamics of the snail intermediate host and the best possible methods of their control, (3) in vitro and in vivo testing of chemotherapeutic compounds using sensitive methods, and (4) the identification of novel drug and vaccine candidates using modern molecular markers. This approach may help increase the reliability of chemotherapeutic agents and control nuisance, ultimately reducing the economic losses attributable to the livestock industry around the world. [ABSTRACT FROM AUTHOR]

The involvement of bovine lymphocyte antigen (BoLA) in immune system and its role in susceptibility/resistance to infectious diseases has been extensively studied. However, few studies have been conducted to investigate the association between BoLA and gait scores. Our objective was to investigate whether polymorphisms in BoLA gene are associated with susceptibility of lameness in 435 Chinese Holstein cows. Genotyping of the BoLA-DRB3.2 gene was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with three restriction endonucleases ( BstUI, BstYI and HaeIII). The relationship between the polymorphisms in BoLA-DRB3.2 gene and gait scores was analyzed by least-squares linear model. The gait score was non-significant among all five BstUI-RFLP and BstYI-RFLP genotypes. However, analysis of seven HaeIII-RFLP genotypes revealed a significantly higher gait score for AB genotype than others. In conclusion, BoLA-DRB3.2 may be a candidate gene for lameness susceptibility in Chinese Holstein cows. [ABSTRACT FROM AUTHOR]