1. Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation
- Author
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Emilio Hirsch, Claudia Bearzi, Luigi Naldini, Luca Sala, P Portararo, Gianluigi Condorelli, Antonio Zaza, Claudia Altomare, Roberto Papait, Paola Cattaneo, E Di Pasquale, Roberto Rizzi, Michael V.G. Latronico, Rizzi, R, Di Pasquale, E, Portararo, P, Papait, R, Cattaneo, P, Latronico, M, Altomare, C, Sala, L, Zaza, A, Hirsch, E, Naldini, L, Condorelli, G, and Bearzi, C
- Subjects
Octamer Transcription Factor-3 ,Cellular differentiation ,Cell ,cardiac fibroblasts ,cardiac repair ,cardiomyocytes ,iPS cells ,Animals ,Bone Morphogenetic Protein 2 ,Calcium ,Cell Differentiation ,Cells, Cultured ,Cellular Reprogramming ,Embryonic Stem Cells ,Gene Expression Regulation ,Induced Pluripotent Stem Cells ,Karyotyping ,Kruppel-Like Factor 4 ,Kruppel-Like Transcription Factors ,Mice ,Myocytes, Cardiac ,SOXB1 Transcription Factors ,Induced Pluripotent Stem Cell ,0302 clinical medicine ,Nuclear Reprogramming ,Induced pluripotent stem cell ,Kruppel-Like Transcription Factor ,0303 health sciences ,Cultured ,Cell biology ,medicine.anatomical_structure ,Cardiac ,KOSR ,Cells ,Germ layer ,SOXB1 Transcription Factor ,Biology ,03 medical and health sciences ,Embryonic Stem Cell ,medicine ,Epigenetics ,Molecular Biology ,030304 developmental biology ,Myocytes ,Original Paper ,Animal ,Cell Biology ,Embryonic stem cell ,Molecular biology ,030217 neurology & neurosurgery - Abstract
Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation.
- Published
- 2012
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