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1. Analyse humaner Hirnorganoide vom Millimeter- bis zum Nanometerbreich.

Author

Rodriguez-Gatica, Juan Eduardo, Iefremova, Vira, Brüstle, Oliver, Schwarz, Martin Karl, and Kubitscheck, Ulrich

Abstract

We combine tissue expansion and light sheet fluorescence microscopy to analyze brain organoids. It is possible to zoom from a mesoscopic overview to super-resolution in a single imaging session, revealing cellular and subcellular structural details, such as the positions and spacing of pre- and postsynaptic proteins. Light sheet fluorescence expansion microscopy (LSFEM) greatly facilitates the qualitative and quantitative use of organoids in developmental and disease-related studies. [ABSTRACT FROM AUTHOR]

Published
2023
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