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Vocalization in anurans is mainly studied in the context of male-specific advertisement calls, while release calls – characteristic of both sexes – usually attract less attention. Here we compare release calls of both sexes of hybrid Pelophylax esculentus (considering triploid hybrids) and its parental species P. ridibundus in Ukrainian population systems. The principal component analysis resulted in 83.3% of total variance explained by the first three components, while discriminant analysis correctly differentiated 69.6% of frogs only. Triploid male hybrids had intermediate positions between diploid hybrids and parental species. Triploid females had intermediate positions only in terms of temporal parameters because frequency parameters mainly depended on their body size. A significant correlation between peak frequency and body size was shown for females of both taxonomical groups and P. ridibundus males. Although we identified differences between the studied groups, these differences were not unambiguous, with overlapping frequency parameters in males. Including more triploids and the other parental species is important for further analysis. [ABSTRACT FROM AUTHOR]