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RNA editing by adenosine deaminases of the ADAR family can lead to protein recoding, since inosine formed from adenosine in mRNA is complementary to cytosine; the resulting codon editing might introduce amino acid substitutions into translated proteins. Proteome recoding can have functional consequences which have been described in many animals including humans. Using protein recoding database derived from publicly available transcriptome data, we identified for the first time the recoding sites in the zebrafish shotgun proteomes. Out of more than a hundred predicted recoding events, ten substitutions were found in six used datasets. Seven of them were in the AMPA glutamate receptor subunits, whose recoding has been well described, and are conserved among vertebrates. Three sites were specific for zebrafish proteins and were found in the transmembrane receptors astrotactin 1 and neuregulin 3b (proteins involved in the neuronal adhesion and signaling) and in the rims2b gene product (presynaptic membrane protein participating in the neurotransmitter release), respectively. Further studies are needed to elucidate the role of recoding of the said three proteins in the zebrafish. [ABSTRACT FROM AUTHOR]

The self-healing of bio-concrete cracks and pores have been utilised worldwide to improve the properties of bio-concrete using different types of bacteria. Meanwhile, no published research was conducted to heal bio-foamed concrete bricks (B-FCB) pores using Bacillus tequilensis. Previous studies focused on the concentration of bacteria and neglect other factors that could affect the healing process. This research aimed to optimise the healing ratio of B-FCB pores using four factors: B. tequilensis concentration, concrete density, temperature and CO2 concentration. Initial water absorption (IWA) and water absorption (WA) were used as responses in statistical methods, namely, factorial and response surface methodology (RSM). B. tequilensis species was isolated from cement kiln dust, produced in a powder form, then subjected to simulate test using a special medium consisting of foamed concrete materials to check the survival ability in B-FCB. SEM, EDX, and XRD were used to investigate the healing process of B-FCB pores. The results revealed that the decrement ratios of IWA and WA of B-FCB were 52.8% and 29.1% compared to FCB, respectively. SEM results reflect the healing that occurred in B-FCB pores, mostly healed via precipitation of CaCO3 as demonstrated on the XRD results. [ABSTRACT FROM AUTHOR]

Recovering waste metal without the need for remelting in solid-state recycling of metal chips can create green production. The overall process of solid-state recycling should be run at the lowest possible cost to remain competitive. High temperature and prolonged preheating time for billet's homogenization in hot extrusion to consolidate the chips conflicts with the aim of minimizing energy usage. Therefore, optimizing the effect of preheating temperature and time prior to hot extrusion is important. This study investigates the effects of preheating temperature and preheating time on the extrudates' quality. Milling chips of AA6061 were cold compacted and hot extruded through a flat-face die using preheating temperatures of 400, 500, and 550 °C for 1-6 h of preheating time. The mechanical and physical properties and microstructure of the extruded profiles were compared. The results revealed that higher acceptable strength and ductility were obtained at 500 °C with 2 h of preheating time. On top of that, temperature increase was the main criterion that results in the highest tensile strength; nevertheless, it was subjected to trade-off in ductility. The profile extruded at 500 and 550 °C had gained a close tensile strength. The study includes the prediction of the chip's welding quality through the damage evolution on the extrudate's structure. It was implemented with the help of DEFORMTM 3D finite element method (FEM) software, and the normalized Cockcroft and Latham (C & L) fracture criterion was chosen. The results of the simulations were compared and validated by the experimental results. [ABSTRACT FROM AUTHOR]