32 results on '"Sharma, Shailendra"'
Search Results
2. Au Cluster-derived Electrocatalysts for CO2 Reduction.
- Author
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Sharma, Shailendra Kumar, Ahangari, Hani Taleshi, Johannessen, Bernt, Golovko, Vladimir B., and Marshall, Aaron T.
- Abstract
Metal clusters often exhibit superior chemical, electronic, and geometrical properties and can show exciting catalytic performance. The catalytic behaviour of the clusters is strongly affected by their size and composition, offering unique opportunities to fine-tune such materials for a specific application. In this study, atomically precise [Au
6 (dppp)4 ](NO3 )2 , [Au9 (PPh3 )8 ](NO3 )3 , [Au13 (dppe)5 Cl2 ]Cl3 and Au101 (PPPh3 )21 Cl5 clusters were synthesised, characterised and their activity for electrocatalytic CO2 reduction is compared. These Au clusters were deposited onto carbon paper to serve as the cathode for the electrochemical reduction of CO2 . The experimental studies suggest that the clusters remain intact upon deposition on the carbon paper but undergo agglomeration during CO2 electrolysis. The cluster-based catalysts demonstrated high selectivity (75%—90%) for CO production over hydrogen evolution reaction. Upon calcination, the activity of the cluster-based electrodes decreases, which can be attributed to the agglomeration of small clusters into larger bulk-like nanoparticles, as suggested by XPS, XAS and SEM. A series of phosphine-protected gold clusters supported on carbon paper were studied as CO2 RR electrocatalysts. The CO2 RR activity was dependent on their size and ligand density. Thermal annealing of the catalysts invariably lowered CO selectivity due to agglomeration of the clusters into larger nanoparticles. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
3. GWAS scans of cereal cyst nematode (Heterodera avenae) resistance in Indian wheat germplasm.
- Author
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Singh, Vikas Kumar, Chaturvedi, Deepti, Pundir, Saksham, Kumar, Deepak, Sharma, Rajiv, Kumar, Sundeep, Sharma, Shiveta, and Sharma, Shailendra
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HETERODERA ,CYST nematodes ,WHEAT ,GENOME-wide association studies ,GERMPLASM ,ARACHNOID cysts ,SOYBEAN cyst nematode ,ROOT-knot nematodes - Abstract
Significant yield losses in major cereal-growing regions around the world have been linked to cereal cyst nematodes (Heterodera spp.). Identifying and deploying natural sources of resistance is of utmost importance due to increasing concerns associated with chemical methods over the years. We screened 141 diverse wheat genotypes collected from pan-Indian wheat cultivation states for nematode resistance over two years, alongside two resistant (Raj MR1, W7984 (M6)) and two susceptible (WH147, Opata M85) checks. We performed genome-wide association analysis using four single-locus models (GLM, MLM, CMLM, and ECMLM) and three multi-locus models (Blink, FarmCPU, and MLMM). Single locus models identified nine significant MTAs (−log10 (P) > 3.0) on chromosomes 2A, 3B, and 4B whereas, multi-locus models identified 11 significant MTAs on chromosomes 1B, 2A, 3B, 3D and 4B. Single and multi-locus models identified nine common significant MTAs. Candidate gene analysis identified 33 genes like F-box-like domain superfamily, Cytochrome P450 superfamily, Leucine-rich repeat, cysteine-containing subtype Zinc finger RING/FYVE/PHD-type, etc., having a putative role in disease resistance. Such genetic resources can help to reduce the impact of this disease on wheat production. Additionally, these results can be used to design new strategies for controlling the spread of H. avenae, such as the development of resistant varieties or the use of resistant cultivars. Finally, the obtained results can also be used to identify new sources of resistance to this pathogen and develop novel control methods. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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4. Identification of genomic regions associated with cereal cyst nematode (Heterodera avenae Woll.) resistance in spring and winter wheat.
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Chaturvedi, Deepti, Pundir, Saksham, Singh, Vikas Kumar, Kumar, Deepak, Sharma, Rajiv, Röder, Marion S., Sharma, Shiveta, and Sharma, Shailendra
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WHEAT ,ARACHNOID cysts ,WINTER wheat ,HETERODERA ,PLANT defenses ,GENOME-wide association studies ,AGRICULTURAL productivity ,CYSTS (Pathology) - Abstract
Cereal cyst nematode (CCN) is a major threat to cereal crop production globally including wheat (Triticum aestivum L.). In the present study, single-locus and multi-locus models of Genome-Wide Association Study (GWAS) were used to find marker trait associations (MTAs) against CCN (Heterodera avenae) in wheat. In total, 180 wheat accessions (100 spring and 80 winter types) were screened against H. avenae in two independent years (2018/2019 "Environment 1" and 2019/2020 "Environment 2") under controlled conditions. A set of 12,908 SNP markers were used to perform the GWAS. Altogether, 11 significant MTAs, with threshold value of −log10 (p-values) ≥ 3.0, were detected using 180 wheat accessions under combined environment (CE). A novel MTA (wsnp_Ex_c53387_56641291) was detected under all environments (E1, E2 and CE) and considered to be stable MTA. Among the identified 11 MTAs, eight were novel and three were co-localized with previously known genes/QTLs/MTAs. In total, 13 putative candidate genes showing differential expression in roots, and known to be involved in plant defense mechanisms were reported. These MTAs could help us to identify resistance alleles from new sources, which could be used to identify wheat varieties with enhanced CCN resistance. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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5. Validation of resistance to cereal cyst nematode (Heterodera avenae) and yield performance study in doubled haploid lines of wheat (Triticum aestivum L.).
- Author
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Pundir, Saksham, Singh, Vikas Kumar, Kumar, Satish, Chaturvedi, Deepti, Kumar, Deepak, Kanwar, Rambir Singh, Kumar, Ashok, Börner, Andreas, Sharma, Shiveta, and Sharma, Shailendra
- Abstract
Cereal cyst nematode (CCN), Heterodera avenae, is an important plant parasitic nematode in major wheat growing states of northern India. Recently, we detected a major QTL, "QCcn.ha-2D" using 114 doubled haploid (DH) lines of wheat obtained from a cross between CIMMYT synthetic hexaploid wheat line W7984 (also known as M6) and the hard red spring wheat Opata M85. In the present study, resistance nature of selected DH lines, harboring this major QTL, was validated for H. avenae resistance under controlled and natural conditions. Different developmental stages of nematode were also studied through staining of infected resistant and susceptible plant roots. In contrast to susceptible DH line roots, staining of resistant DH line roots showed decrease in total number of juveniles, J3s with absence of J4 stage juveniles. In comparison to known resistant parent, most of the resistant DH lines showed better biological yield per plot, grain yield per plot and harvest index, measured over two years. Present study indicates that selected DH lines are promising prebreeding material to introduce novel genetic variation for H. avenae resistance in cultivated wheat. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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6. Predictive Control-Based DSTATCOM with a Multi-Criterion Decision-Making Method.
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Srikakolapu, Jayadeep, Arya, Sabha Raj, Maurya, Rakesh, and Sharma, Shailendra
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- 2022
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7. QTL mapping for resistance against cereal cyst nematode (Heterodera avenae Woll.) in wheat (Triticum aestivum L.).
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Pundir, Saksham, Sharma, Rajiv, Kumar, Deepak, Singh, Vikas Kumar, Chaturvedi, Deepti, Kanwar, Rambir Singh, Röder, Marion S., Börner, Andreas, Ganal, Martin W., Gupta, Pushpendra Kumar, Sharma, Shailendra, and Sharma, Shiveta
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HETERODERA ,PLANT nematodes ,WHEAT breeding ,WHEAT ,GERMPLASM ,CYSTS (Pathology) ,ARACHNOID cysts - Abstract
The resistance to cereal cyst nematode (Heterodera avenae Woll.) in wheat (Triticum aestivum L.) was studied using 114 doubled haploid lines from a novel ITMI mapping population. These lines were screened for nematode infestation in a controlled environment for two years. QTL-mapping analyses were performed across two years (Y1 and Y2) as well as combining two years (CY) data. On the 114 lines that were screened, a total of 2,736 data points (genotype, batch or years, and replication combinations) were acquired. For QTL analysis, 12,093 markers (11,678 SNPs and 415 SSRs markers) were used, after filtering the genotypic data, for the QTL mapping. Composite interval mapping, using Haley-Knott regression (hk) method in R/QTL, was used for QTL analysis. In total, 19 QTLs were detected out of which 13 were novel and six were found to be colocalized or nearby to previously reported Cre genes, QTLs or MTAs for H. avenae or H. filipjevi. Nine QTLs were detected across all three groups (Y1, Y2 and CY) including a significant QTL "QCcn.ha-2D" on chromosome 2D that explains 23% of the variance. This QTL colocalized with a previously identified Cre3 locus. Novel QTL, QCcn.ha-2A, detected in the present study could be the possible unreported homeoloci to QCcn.ha-2D, QCcn.ha-2B.1 and QCcn.ha-2B.2. Six significant digenic epistatic interactions were also observed. In addition, 26 candidate genes were also identified including genes known for their involvement in PPNs (plant parasitic nematodes) resistance in different plant species. In-silico expression of putative candidate genes showed differential expression in roots during specific developmental stages. Results obtained in the present study are useful for wheat breeding to generate resistant genetic resources against H. avenae. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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8. Energy management in microgrid employing unit commitment considering diverse system uncertainties.
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Mandloi, Toshi, Sharma, Shailendra Kumar, and Choube, S. C.
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PARTICLE swarm optimization , *ENERGY demand management , *RENEWABLE energy sources , *OPTIMIZATION algorithms , *MARKET prices - Abstract
An approach for microgrid energy management using unit commitment (UC) is presented in this research paper. In energy management, the aim is to identify the dispatchable generating units to uncover the most efficient generation scheduling from the set of available generators. The power generation landscape undergoes significant changes due to fluctuations in demand, renewable energy sources, and variations in price/kWH. An efficient UC solution is crucial for a reliable and cost-effective electricity supply. This study delves into uncertainties such as the impact of market prices on unit commitment decisions, aiming to optimize UC considering uncertainties of electrical demand and wind power generation. A model using a normal probability density function is introduced to address uncertain market prices, electrical demand, and wind energy sources. The proposed war strategy optimization algorithm (WSOA) and the modified WSOA (MWSOA) are presented to show their superiority over other optimizing algorithms like particle swarm optimization (PSO), gray wolf optimization (GWO), and modified GWO (MGWO) in achieving cost-effective and reliable UC solutions. The investigated research offers valuable perception into the application of MWSOA and WSOA in unit commitment, enhancing the efficiency of power generation in smart grid environments. Particularly in cases involving uncertain market prices, the percentage increase in profitability achieved with MWSOA is compared to WSOA, MGWO, GWO, and PSO. Notably, profitability increases to 8.91%, 12.15%, 13.67%, and 14.85%, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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9. Reduced Sensor-Based Control of Unified Power Quality Conditioner.
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Sharma, Anirudh, Sharma, Shailendra Kumar, Singh, Bhim, and Shah, Priyank
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- 2022
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10. GWAS for main effects and epistatic interactions for grain morphology traits in wheat.
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Malik, Parveen, Kumar, Jitendra, Sharma, Shiveta, Meher, Prabina Kumar, Balyan, Harindra Singh, Gupta, Pushpendra Kumar, and Sharma, Shailendra
- Abstract
In the present study in wheat, GWAS was conducted for identification of marker trait associations (MTAs) for the following six grain morphology traits: (1) grain cross-sectional area (GCSA), (2) grain perimeter (GP), (3) grain length (GL), (4) grain width (GWid), (5) grain length–width ratio (GLWR) and (6) grain form-density (GFD). The data were recorded on a subset of spring wheat reference set (SWRS) comprising 225 diverse genotypes, which were genotyped using 10,904 SNPs and phenotyped for two consecutive years (2017–2018, 2018–2019). GWAS was conducted using five different models including two single-locus models (CMLM, SUPER), one multi-locus model (FarmCPU), one multi-trait model (mvLMM) and a model for Q x Q epistatic interactions. False discovery rate (FDR) [P value -log10(p) ≥ 5] and Bonferroni correction [P value -log10(p) ≥ 6] (corrected p value < 0.05) were applied to eliminate false positives due to multiple testing. This exercise gave 88 main effect and 29 epistatic MTAs after FDR and 13 main effect and 6 epistatic MTAs after Bonferroni corrections. MTAs obtained after Bonferroni corrections were further utilized for identification of 55 candidate genes (CGs). In silico expression analysis of CGs in different tissues at different parts of the seed at different developmental stages was also carried out. MTAs and CGs identified during the present study are useful addition to available resources for MAS to supplement wheat breeding programmes after due validation and also for future strategic basic research. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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11. Meta-QTLs, ortho-MetaQTLs and candidate genes for grain Fe and Zn contents in wheat (Triticum aestivum L.).
- Author
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Singh, Rakhi, Saripalli, Gautam, Gautam, Tinku, Kumar, Anuj, Jan, Irfat, Batra, Ritu, Kumar, Jitendra, Kumar, Rahul, Balyan, Harindra Singh, Sharma, Shailendra, and Gupta, Pushpendra Kumar
- Abstract
Majority of cereals are deficient in essential micronutrients including grain iron (GFe) and grain zinc (GZn), which are therefore the subject of research involving biofortification. In the present study, 11 meta-QTLs (MQTLs) including nine novel MQTLs for GFe and GZn contents were identified in wheat. Eight of these 11 MQTLs controlled both GFe and GZn. The confidence intervals of the MQTLs were narrower (0.51–15.75 cM) relative to those of the corresponding QTLs (0.6 to 55.1 cM). Two ortho-MQTLs involving three cereals (wheat, rice and maize) were also identified. Results of MQTLs were also compared with the results of earlier genome wide association studies (GWAS). As many as 101 candidate genes (CGs) underlying MQTLs were also identified. Twelve of these CGs were prioritized; these CGs encoded proteins with important domains (zinc finger, RING/FYVE/PHD type, flavin adenine dinucleotide linked oxidase, etc.) that are involved in metal ion binding, heme binding, iron binding, etc. qRT-PCR analysis was conducted for four of these 12 prioritized CGs using genotypes which have differed for GFe and GZn. Significant differential expression in these genotypes was observed at 14 and 28 days after anthesis. The MQTLs/CGs identified in the present study may be utilized in marker-assisted selection (MAS) for improvement of GFe/GZn contents and also for understanding the molecular basis of GFe/GZn homeostasis in wheat. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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12. Correlation in the Near and Far Field of Compressible Jet to Identify Noise Source Characteristics.
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Nikam, Shailesh Ravindra and Sharma, Shailendra
- Abstract
A noise source characteristic of an unheated compressible jet is experimentally investigated for the jet with exit Mach number 0.8. Measurements of acoustic pressure fluctuations were performed in the near-field and far-field independently to identify noise source characteristics using the correlation technique. Near-field and far-field acoustic pressure fluctuations were also measured simultaneously to identify the acoustic component in near-field pressure fluctuations measured. Measurements were taken for the base and chevron nozzle with the nozzle exit diameter of 30 mm. From auto-correlation and cross-correlation in the near and far fields, it is observed that region surrounding to mixing layer is dominated by random small scale structures which radiate noise to sideline direction in the far-field. Correlation obtained at downstream of the potential core in the near-field represents the dominance of coherent large scale structure which radiates the noise to the far-field in the aft angle direction. Chevron changes the characteristics of noise sources. The size of the dominant large-scale noise source reduces due to chevron. Coherence of the noise source in the region surrounding to mixing layer reduces due to chevron which is responsible for reducing the energy of the dominant noise sources downstream of the potential core. Near-field far-field cross-correlation confirms that spatial near-field location for radiating acoustic pressure fluctuations more efficiently to the far-field at polar angle 30° is dependent on axial as well as transverse position in the near-field. Chevron reduces the convective velocity of the flow. Chevron also reduces the spatial correlation length scale of the dominant noise source. Chevron changes the acoustic pressure in the near-field thereby shifting the dominant noise source towards the nozzle exit. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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13. Chemically synthesized Ti-doped SnS2 thin films as intermediate band gap material for solar cell application.
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Rajwar, Birendra Kumar and Sharma, Shailendra Kumar
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THIN films , *BAND gaps , *SOLAR cells , *HALL effect , *SOLAR spectra , *X-ray photoelectron spectroscopy - Abstract
Herein, we report the structural, morphological, optical and electrical properties of undoped and Ti-doped SnS2 thin films deposited on glass substrate by sol–gel spin coating technique. Grazing-Incidence X-ray diffraction and Raman analyses confirm the formation of hexagonal phase of as-deposited films. The structural parameters were estimated through GI-XRD data and found to be varying with Ti4+ concentration. Field effect scanning electron microscopy images show as-grown spherical nanoparticles with uniform deposition on the entire glass substrate. The UV–Vis absorption spectra were recorded to explore the optical properties of films. The analysis of data revealed the semiconductor nature of as-deposited films with a band gap of 2.24 eV in case of undoped SnS2. An intermediate band was also observed at 5 and 20 mol% Ti4+ concentration which directly indicates the extension of light absorption ability from visible to near infrared region of the solar spectrum. The X-ray photoelectron spectroscopy analysis was performed to confirm the existence of constituent elements in the as-deposited films with their oxidation state. The electrical properties were explored by I–V and Hall Effect measurements. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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14. Single-trait, multi-locus and multi-trait GWAS using four different models for yield traits in bread wheat.
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Malik, Parveen, Kumar, Jitendra, Singh, Sahadev, Sharma, Shiveta, Meher, Prabina Kumar, Sharma, Mukesh Kumar, Roy, Joy Kumar, Sharma, Pradeep Kumar, Balyan, Harindra Singh, Gupta, Pushpendra Kumar, and Sharma, Shailendra
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GENOME-wide association studies ,BONFERRONI correction ,BREAD - Abstract
A genome-wide association study (GWAS) for 10 yield and yield component traits was conducted using an association panel comprising 225 diverse spring wheat genotypes. The panel was genotyped using 10,904 SNPs and evaluated for three years (2016–2019), which constituted three environments (E1, E2 and E3). Heritability for different traits ranged from 29.21 to 97.69%. Marker-trait associations (MTAs) were identified for each trait using data from each environment separately and also using BLUP values. Four different models were used, which included three single trait models (CMLM, FarmCPU, SUPER) and one multi-trait model (mvLMM). Hundreds of MTAs were obtained using each model, but after Bonferroni correction, only 6 MTAs for 3 traits were available using CMLM, and 21 MTAs for 4 traits were available using FarmCPU; none of the 525 MTAs obtained using SUPER could qualify after Bonferroni correction. Using BLUP, 20 MTAs were available, five of which also figured among MTAs identified for individual environments. Using mvLMM model, after Bonferroni correction, 38 multi-trait MTAs, for 15 different trait combinations were available. Epistatic interactions involving 28 pairs of MTAs were also available for seven of the 10 traits; no epistatic interactions were available for GNPS, PH, and BYPP. As many as 164 putative candidate genes (CGs) were identified using all the 50 MTAs (CMLM, 3; FarmCPU, 9; mvLMM, 6, epistasis, 21 and BLUP, 11 MTAs), which ranged from 20 (CMLM) to 66 (epistasis) CGs. In-silico expression analysis of CGs was also conducted in different tissues at different developmental stages. The information generated through the present study proved useful for developing a better understanding of the genetics of each of the 10 traits; the study also provided novel markers for marker-assisted selection (MAS) to be utilized for the development of wheat cultivars with improved agronomic traits. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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- View/download PDF
15. Genome-wide association study in hexaploid wheat identifies novel genomic regions associated with resistance to root lesion nematode (Pratylenchus thornei).
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Kumar, Deepak, Sharma, Shiveta, Sharma, Rajiv, Pundir, Saksham, Singh, Vikas Kumar, Chaturvedi, Deepti, Singh, Bansa, Kumar, Sundeep, and Sharma, Shailendra
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WHEAT ,PRATYLENCHUS ,NEMATOCIDES ,LOCUS in plant genetics ,SINGLE nucleotide polymorphisms ,GENOME-wide association studies - Abstract
Root lesion nematode (RLN; Pratylenchus thornei) causes extensive yield losses in wheat worldwide and thus pose serious threat to global food security. Reliance on fumigants (such as methyl bromide) and nematicides for crop protection has been discouraged due to environmental concerns. Hence, alternative environment friendly control measures like finding and deployment of resistance genes against Pratylenchus thornei are of significant importance. In the present study, genome-wide association study (GWAS) was performed using single-locus and multi-locus methods. In total, 143 wheat genotypes collected from pan-Indian wheat cultivation states were used for nematode screening. Genotypic data consisted of > 7K SNPs with known genetic positions on the high-density consensus map was used for association analysis. Principal component analysis indicated the existence of sub-populations with no major structuring of populations due to the origin. Altogether, 25 significant marker trait associations were detected with − log10 (p value) > 4.0. Three large linkage disequilibrium blocks and the corresponding haplotypes were found to be associated with significant SNPs. In total, 37 candidate genes with nine genes having a putative role in disease resistance (F-box-like domain superfamily, Leucine-rich repeat, cysteine-containing subtype, Cytochrome P450 superfamily, Zinc finger C2H2-type, RING/FYVE/PHD-type, etc.) were identified. Genomic selection was conducted to investigate how well one could predict the phenotype of the nematode count without performing the screening experiments. Prediction value of r = 0.40 to 0.44 was observed when 56 to 70% of the population was used as a training set. This is the first report where GWAS has been conducted to find resistance against root lesion nematode (P. thornei) in Indian wheat germplasm. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
16. Biofortification and bioavailability of Zn, Fe and Se in wheat: present status and future prospects.
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Gupta, P. K., Balyan, H. S., Sharma, Shailendra, and Kumar, Rahul
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BIOFORTIFICATION ,MICRONUTRIENTS ,BIOAVAILABILITY ,WHEAT ,WHEAT breeding ,PHYTIC acid ,TRANSGENES ,GENETICS - Abstract
Key message: Knowledge of genetic variation, genetics, physiology/molecular basis and breeding (including biotechnological approaches) for biofortification and bioavailability for Zn, Fe and Se will help in developing nutritionally improved wheat. Biofortification of wheat cultivars for micronutrients is a priority research area for wheat geneticists and breeders. It is known that during breeding of wheat cultivars for productivity and quality, a loss of grain micronutrient contents occurred, leading to decline in nutritional quality of wheat grain. Keeping this in view, major efforts have been made during the last two decades for achieving biofortification and bioavailability of wheat grain for micronutrients including Zn, Fe and Se. The studies conducted so far included evaluation of gene pools for contents of not only grain micronutrients as above, but also for phytic acid (PA) or phytate and phytase, so that, while breeding for the micronutrients, bioavailability is also improved. For this purpose, QTL interval mapping and GWAS were carried out to identify QTLs/genes and associated markers that were subsequently used for marker-assisted selection (MAS) during breeding for biofortification. Studies have also been conducted to understand the physiology and molecular basis of biofortification, which also allowed identification of genes for uptake, transport and storage of micronutrients. Transgenics using transgenes have also been produced. The breeding efforts led to the development of at least a dozen cultivars with improved contents of grain micronutrients, although land area occupied by these biofortified cultivars is still marginal. In this review, the available information on different aspects of biofortification and bioavailability of micronutrients including Zn, Fe and Se in wheat has been reviewed for the benefit of those, who plan to start work or already conducting research in this area. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
17. Solvent-dependent structural and optical properties of Cu2MSnS4 synthesized via ultrasonic-assisted sol–gel method.
- Author
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Rajwar, Birendra Kumar and Sharma, Shailendra Kumar
- Abstract
Herein, structural and optical properties of Cu
2 MSnS4 (M = Co and Fe) nanoparticles synthesized by ultrasonic-assisted sol–gel method using two different solvents are studied. XRD study shows the nanoparticles with tetragonal kesterite structure and crystallite size in the range of 4–7 nm. XPS study reveals the oxidation state of constituent elements. The optical band gap and textural properties like surface area and pore size are estimated through UV–VIS–NIR and nitrogen adsorption–desorption analyses. Highlights: Quaternary Cu2 MSnS4 (M = Co & Fe) nanoparticles were successfully synthesized using two different solvents by ultrasonic assisted sol–gel method. The as-synthesized material shows good optical response from UV to NIR region of the solar spectrum. The good optical response along with its large BET surface area to volume ratio suggests that the synthesized material can have potential application as an absorber layer in a thin-film solar cell. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
18. Genetics of yield, abiotic stress tolerance and biofortification in wheat (Triticum aestivum L.).
- Author
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Gupta, Pushpendra Kumar, Balyan, Harindra Singh, Sharma, Shailendra, and Kumar, Rahul
- Subjects
BIOFORTIFICATION ,ABIOTIC stress ,GENETICS ,DROUGHT tolerance ,WHEAT ,LITERATURE reviews - Abstract
Key message: A review of the available literature on genetics of yield and its component traits, tolerance to abiotic stresses and biofortification should prove useful for future research in wheat in the genomics era. The work reviewed in this article mainly covers the available information on genetics of some important quantitative traits including yield and its components, tolerance to abiotic stresses (heat, drought, salinity and pre-harvest sprouting = PHS) and biofortification (Fe/Zn and phytate contents with HarvestPlus Program) in wheat. Major emphasis is laid on the recent literature on QTL interval mapping and genome-wide association studies, giving lists of known QTL and marker-trait associations. Candidate genes for different traits and the cloned and characterized genes for yield traits along with the molecular mechanism are also described. For each trait, an account of the present status of marker-assisted selection has also been included. The details of available results have largely been presented in the form of tables; some of these tables are included as supplementary files. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
19. Genetic diversity and population structure analysis of Indian garlic (Allium sativum L.) collection using SSR markers.
- Author
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Kumar, Mukesh, Rakesh Sharma, V., Kumar, Vipin, Sirohi, Ujjawal, Chaudhary, Veena, Sharma, Shiveta, Saripalli, Gautam, Naresh, R. K., Yadav, Hemant Kumar, and Sharma, Shailendra
- Abstract
Genetic diversity was assessed among 53 Indian garlic accessions using SSR markers. Initially, 24 SSR primer pairs were used for screening three selected garlic accessions. Out of 24 SSR primer pairs, 10 primer pairs which consistently showed good amplification and polymorphism were selected for DNA profiling. SSR primer pairs showed PIC values ranging from 0.30 to 0.99. Based on AMOVA we found that the greater part of the genetic diversity was expected due to intra population with 84% variation and only 16% of variation was due to among populations suggesting presence of genetic structure. The results of cluster analysis and principal component analysis largely correspond to each other. Population structure analysis revealed genetic differentiation of accessions. The results of present study revealed existence of significant variability in Indian garlic germplasm. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
20. Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.
- Author
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Sarkar, Anita, Bale, Shridhar, Behrens, Anna-Janina, Kumar, Sonu, Sharma, Shailendra Kumar, de Val, Natalia, Pallesen, Jesper, Irimia, Adriana, Diwanji, Devan C., Stanfield, Robyn L., Ward, Andrew B., Crispin, Max, Wyatt, Richard T., and Wilson, Ian A.
- Subjects
BINDING sites ,CORONAVIRUSES ,PROTEIN structure ,CRYSTAL structure ,HIV ,EPITOPES ,GLYCANS - Abstract
Furin cleavage of the HIV envelope glycoprotein is an essential step for cell entry that enables formation of well-folded, native-like glycosylated trimers, releases constraints on the fusion peptide, and limits enzymatic processing of the N-glycan shield. Here, we show that a cleavage-independent, stabilized, soluble Env trimer mimic (BG505 NFL.664) exhibits a "closed-form", native-like, prefusion conformation akin to furin-cleaved Env trimers. The crystal structure of BG505 NFL.664 at 3.39 Å resolution with two potent bNAbs also identifies the full epitopes of PGV19 and PGT122 that target the receptor binding site and N332 supersite, respectively. Quantitative site-specific analysis of the glycan shield reveals that native-like glycan processing is maintained despite furin-independent maturation in the secretory pathway. Thus, cleavage-independent NFL Env trimers exhibit quaternary protein and carbohydrate structures similar to the native viral spike that further validate their potential as vaccine immunogen candidates. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
21. Assessment of genetic diversity and population structure in gladiolus (<italic>Gladiolus hybridus</italic> Hort.) by ISSR markers.
- Author
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Chaudhary, Veena, Kumar, Mukesh, Sharma, Shailendra, Kumar, Navneet, Kumar, Vipin, Yadav, Hemant Kumar, Sharma, Shiveta, and Sirohi, Ujjwal
- Abstract
ISSR (Inter simple sequence repeat) markers were used to assess the genetic diversity and population structure in 53 indigenous and exotic genotypes of gladiolus (
Gladiolus hybridus Hort.). Molecular markers analysis showed PIC ranges from 0.42 (ISSR 861) to 0.99 (ISSR 855, ISSR 856 and ISSR 889) with an average 0.812, marker index ranged from 0.99 (ISSR 889) to 9.26 (ISSR 851) with an average 4.66 and resolving power of the primers ranged from 0.03 (ISSR 889) to 11.58 (ISSR 861) with an average value 3.80. The dendrogram based UPGMA clustering showed that all the 53 genotypes grouped into three main clusters. Nei’s gene diversity (Na) varied from 0.929 to 1.717, effective number of alleles (Ne) varied from 1.262 to 1.369, Shannon’s information index (I) ranged from 0.251 to 0.359 and gene diversity (He) was in the range from 0.167 to 0.229. Population structure analysis revealed three groups in which 32 genotypes were admixture types. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
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22. Higher prevalence of unrecognized kidney disease at high altitude.
- Author
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Hurtado-Arestegui, Abdias, Plata-Cornejo, Raul, Cornejo, Arturo, Mas, Guiliana, Carbajal, Luz, Sharma, Shailendra, Swenson, Erik R., Johnson, Richard J., and Pando, Jackelina
- Published
- 2018
- Full Text
- View/download PDF
23. Reusable graphene oxide nanofibers for enhanced photocatalytic activity: a detailed mechanistic study.
- Author
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Sharma, Shailendra, Sokhi, Shivali, Balomajumder, Chandrajit, and Satapathi, Soumitra
- Subjects
- *
NANOFIBERS , *PHOTOCATALYSIS , *GRAPHENE oxide , *WATER pollution , *DYES & dyeing , *GENETIC toxicology - Abstract
Water pollution due to indiscriminate disposal of industrial dyes poses serious environmental hazards nationally and internationally. Some of the dyes are potentially carcinogenic and may induce mutagenicity and genotoxicity. Recently, graphene-based nanocomposite has attracted considerable interest for photocatalysis-based wastewater treatment. Here, we report the production of graphene-oxide-based hydrophobic PAN/GO nanofibers using electrospinning technique for photocatalytic degradation of Rhodamine 6G dye under natural sunlight illumination. The synthesized nanofibers were characterized using X-ray diffraction, EDX, field emission scanning electron microscopy and FTIR spectroscopy. Dye removal efficiency was investigated by monitoring UV-Vis absorption intensity over time. Structural change in dye was studied using FTIR analysis. Kinetics of dye degradation reaction was monitored through pseudo-first-order and pseudo-second-order kinetics model. Effects of nanofiber weight and initial dye concentration on the degradation efficiency were investigated in detail. Reusability and stability of these synthesized nanofibers in dye solution have been studied using scanning electron microscopy and FTIR spectroscopy. A comparative study for dye degradation was also performed using TiO-coated nanofibers under visible light and UV light illumination. These large-area reusable graphene oxide nanofibers provide a scalable and novel route for photocatalytic degradation of carcinogenic dyes from industrial water. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
24. Comparative QTL analysis of root lesion nematode resistance in barley.
- Author
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Galal, Ahmed, Sharma, Shiveta, Abou-Elwafa, Salah, Sharma, Shailendra, Kopisch-Obuch, Friedrich, Laubach, Eberhard, Perovic, Dragan, Ordon, Frank, and Jung, Christian
- Subjects
PLANT roots ,BARLEY disease & pest resistance ,PLANT chromosomes ,PRATYLENCHUS penetrans ,PLANT gene mapping ,BIOMARKERS ,PLANTS - Abstract
Key message: This study demonstrates for the first time that resistance to different root lesion nematodes ( P. neglectus and P. penetrans ) is controlled by a common QTL. A major resistance QTL ( Rlnnp6H ) has been mapped to chromosome 6H using two independent barley populations. Abstract: Root lesion nematodes ( Pratylenchus spp.) are important pests in cereal production worldwide. We selected two doubled haploid populations of barley (Igri × Franka and Uschi × HHOR 3073) and infected them with Pratylenchus penetrans and Pratylenchus neglectus. Nematode multiplication rates were measured 7 or 10 weeks after infection. In both populations, continuous phenotypic variations for nematode multiplication rates were detected indicating a quantitative inheritance of resistance. In the Igri × Franka population, four P. penetrans resistance QTLs were mapped with 857 molecular markers on four linkage groups (2H, 5H, 6H and 7H). In the Uschi × HHOR 3073 population, eleven resistance QTLs ( P. penetrans and P. neglectus) were mapped with 646 molecular markers on linkage groups 1H, 3H, 4H, 5H, 6H and 7H. A major resistance QTL named Rlnnp6H (LOD score 6.42-11.19) with a large phenotypic effect (27.5-36.6 %) for both pests was mapped in both populations to chromosome 6H. Another resistance QTL for both pests was mapped on linkage group 5H (Igri × Franka population). These data provide first evidence for common resistance mechanisms against different root lesion nematode species. The molecular markers are a powerful tool for the selection of resistant barley lines among segregating populations because resistance tests are time consuming and laborious. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
25. Hyponatraemia: more than just a marker of disease severity?
- Author
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Schrier, Robert W., Sharma, Shailendra, and Shchekochikhin, Dmitry
- Subjects
- *
HYPONATREMIA , *SODIUM metabolism disorders , *ELECTROLYTES , *PROGNOSIS , *HEART failure , *CIRRHOSIS of the liver - Abstract
Hyponatraemia--the most common serum electrolyte disorder--has also emerged as an important marker of the severity and prognosis of important diseases such as heart failure and cirrhosis. Acute hyponatraemia can cause severe encephalopathy, but the rapid correction of chronic hyponatraemia can also profoundly impair brain function and even cause death. With the expanding elderly population and the increased prevalence of hyponatraemia in this segment of society, prospective studies are needed to examine whether correcting hyponatraemia in the elderly will diminish cognitive impairment, improve balance and reduce the incidence of falls and fractures. Given that polypharmacy is also common in the elderly population, the various medications that may stimulate arginine vasopressin release and/or enhance the hormone's action to increase water absorption must also be taken into consideration. Whether hyponatraemia in a patient with cancer is merely a marker of poor prognosis or whether its presence may alter the patient's quality of life remains to be examined. In any case, hyponatraemia can no longer be considered as just a biochemical bystander in the ill patient. A systematic diagnostic approach is necessary to determine the specific aetiology of a patient's hyponatraemia. Therapy must then be dictated not only by recognized reversible causes such as advanced hypothyroidism, adrenal insufficiency, diuretics or other medicines, but also by whether the hyponatraemia occurred acutely or chronically. Information is emerging that the vast majority of cases of hyponatraemia are caused by the nonosmotic release of arginine vasopressin. Now that vasopressin V2-receptor blockers are available, a new era of clinical investigation is necessary to examine whether hyponatraemia is just a marker of severe disease or whether correction of hyponatraemia could improve a patient's quality of life. Such an approach must involve prospective randomized studies in different groups of patients with hyponatraemia, including those with advanced heart failure, those with cirrhosis, patients with cancer, and the elderly. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
26. Broad and potent neutralization of HIV-1 by a gp41-specific human antibody.
- Author
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Huang, Jinghe, Ofek, Gilad, Laub, Leo, Louder, Mark K., Doria-Rose, Nicole A., Longo, Nancy S., Imamichi, Hiromi, Bailer, Robert T., Chakrabarti, Bimal, Sharma, Shailendra K., Alam, S. Munir, Wang, Tao, Yang, Yongping, Zhang, Baoshan, Migueles, Stephen A., Wyatt, Richard, Haynes, Barton F., Kwong, Peter D., Mascola, John R., and Connors, Mark
- Subjects
MONOCLONAL antibodies ,NEUTRALIZATION (Chemistry) ,HIV ,PHOSPHOLIPIDS ,CELL membranes ,ARGININE ,LYSINE ,VIRAL vaccines - Abstract
Characterization of human monoclonal antibodies is providing considerable insight into mechanisms of broad HIV-1 neutralization. Here we report an HIV-1 gp41 membrane-proximal external region (MPER)-specific antibody, named 10E8, which neutralizes ?98% of tested viruses. An analysis of sera from 78 healthy HIV-1-infected donors demonstrated that 27% contained MPER-specific antibodies and 8% contained 10E8-like specificities. In contrast to other neutralizing MPER antibodies, 10E8 did not bind phospholipids, was not autoreactive, and bound cell-surface envelope. The structure of 10E8 in complex with the complete MPER revealed a site of vulnerability comprising a narrow stretch of highly conserved gp41-hydrophobic residues and a critical arginine or lysine just before the transmembrane region. Analysis of resistant HIV-1 variants confirmed the importance of these residues for neutralization. The highly conserved MPER is a target of potent, non-self-reactive neutralizing antibodies, suggesting that HIV-1 vaccines should aim to induce antibodies to this region of HIV-1 envelope glycoprotein. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
27. Sequence variation in the barley genes encoding sucrose synthase I and sucrose phosphate synthase II, and its association with variation in grain traits and malting quality.
- Author
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Matthies, Inge, Sharma, Shailendra, Weise, Stephan, and Röder, Marion
- Subjects
- *
BARLEY , *SUCROSE , *GRAIN , *CHROMOSOMES , *PLANT genetics - Abstract
Sequence diversity in the two barley ( Hordeum vulgare L.) genes encoding sucrose synthase I ( SSI) and sucrose phosphate synthase II ( SPSII), both of which are involved in sucrose accumulation and grain filling, was studied by partial resequencing of eight reference genotypes and SNP analysis by pyrosequencing in a panel of 94 spring and 96 winter European barley varieties. The resequencing was based on two adjacent SSI fragments of size 880 and 820 bp, and a 2,322 bp SPSII fragment. In the SSI gene, 26 SNPs were present in the larger fragment, and 25 in the smaller one, and 11 of these were exploited to develop high-throughput SNP assays used for haplotype analysis. An association analysis based on either a general or a mixed linear model suggested that the predominant three haplotypes influenced certain components of both kernel and malting quality. However, the level of phenotype/haplotype association shown with the SPSII gene was rather low. SNP variation of SSI was used to map the locus to chromosome 7H. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
28. QTL analysis of root-lesion nematode resistance in barley: 1. Pratylenchus neglectus.
- Author
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Sharma, Shiveta, Sharma, Shailendra, Kopisch-Obuch, Friedrich, Keil, Tobias, Laubach, Eberhard, Stein, Nils, Graner, Andreas, and Jung, Christian
- Subjects
- *
BARLEY disease & pest resistance , *BARLEY varieties , *PLANT nematodes , *PLANT gene mapping , *LOCUS (Genetics) , *PRATYLENCHUS , *GENETIC markers - Abstract
The root-lesion nematode Pratylenchus neglectus can cause severe losses in barley cultivation. Multiplication rates had been found to vary greatly between different barley accessions. Two winter barley cultivars, Igri and Franka, had been found to differ in their ability to resist this parasite. An existing Igri × Franka doubled haploid population was chosen to genetically map resistance genes after artificial inoculation with P. neglectus in the greenhouse and climate chamber. A continuous phenotypic variation was found indicating a quantitative inheritance of P. neglectus resistance. An existing map was enriched by 527 newly developed Diversity Array Technology markers (DArTs). The new genetic linkage map was comprised of 857 molecular markers that cover 1,157 cM on seven linkage groups. Using phenotypic data collected from four different experiments in 3 years, five quantitative trait loci were mapped by composite interval mapping on four (3H, 5H, 6H and 7H) linkage groups. A quantitative trait locus with a large phenotypic effect of 16% and likelihood of odds (LOD) score of 6.35 was mapped on linkage group 3H. The remaining four QTLs were classified as minor or moderate with LOD scores ranging from 2.71 to 3.55 and R values ranging from 8 to 10%. The DNA markers linked to the resistance QTLs should be quite useful for marker-assisted selection in barley breeding because phenotypic selection is limited due to time constraints and labor costs. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
29. Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean.
- Author
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Kumar, Vipin, Sharma, Shailendra, Sharma, Amit Kumar, Sharma, Shiveta, and Bhat, K. Venkataramana
- Subjects
- *
MICROSATELLITE repeats , *BIOMARKERS , *BEAN genetics , *GENETICS , *AGRONOMY - Abstract
Morpho-agronomic traits and microsatellite markers were used to survey genetic diversity in 115 common bean genotypes that included 70 Indian landraces, 24 released varieties and 21 exotic accessions. Twelve morpho-agronomic traits, namely, days to 50% flowering, leaflet length, leaflet width, pod length, pod width, number of pods per plant, days to maturity, seed length, seed width, number of seeds per pod, 100 seed weight and seed yield per plant were studied. Field data of two consecutive years were subjected to multivariate analysis as proposed by Mahalanobis’s D2-statistics, Tochers method of clustering and combined analysis of variance. Seventeen microsatellite markers were also used to examine genetic diversity at molecular level that showed polymorphic information content (PIC) in the range of 0.00–0.684. Dendrograms based on Euclidean distances and UPGMA analysis showed the presence of majority of released varieties into single cluster, which pointed toward their low genetic base in comparison to indigenous landraces and exotic germplasm. Significant correlation existed between morphological genetic distance and microsatellite genetic distance tested by Mantel test ( r = 0.876). [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
30. Genetic diversity in Indian common bean ( Phaseolus vulgaris L.) using random amplified polymorphic DNA markers.
- Author
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Kumar, Vipin, Sharma, Shailendra, Sharma, Amit, Kumar, Mukesh, Sharma, Shiveta, Malik, Sunil, Singh, K., Sanger, R., and Bhat, K.
- Abstract
Genetic diversity of twenty-six common bean ( Phaseolus vulgaris L.) accessions of diverse geographical origin was studied using Random Amplified Polymorphic DNA (RAPD) markers. Fifteen out of forty four primers screened showed polymorphism across present set of genotypes. A total of 124 amplicons were scored using these 15 primers. Ninety five percent of the amplified products showed polymorphism, indicating fair amount of variation at the DNA level among these accessions. Cluster analysis delineated the genotypes in to four groups. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
31. A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute.
- Author
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Mir, Reyazul R., Rustgi, Sachin, Sharma, Shailendra, Singh, Ravinder, Goyal, Aakash K., Kumar, Jitendra, Gaur, Anupama, Tyagi, Akhilesh, Khan, Haseena, Sinha, Mohit K., Balyan, Harindra S., and Gupta, Pushpendra Kumar
- Subjects
JUTE fiber ,GENETIC polymorphisms ,PLANT fibers ,PLANTS ,GENOMICS - Abstract
Jute is one of the most important fibre crops, which is second only to cotton in providing environment-friendly (biodegradable and renewable) ligno-cellulose fibre. In order to improve this largely neglected crop, we conducted a preliminary study involving the following: (i) analysis of nature and extent of the genetic variability for fibre yield and four other related traits in a set of 81 genotypes belonging to two commercially cultivated Corchorus species (45 genotypes of C. olitorius + 36 genotypes of C. capsularis), (ii) development and analysis of a set of simple sequence repeat (SSR) markers from C. olitorius, and (iii) use of a sub-set of SSRs for assessment of genetic diversity in the above set of 81 genotypes. The results suggested quantitative nature of fibre yield and other related traits, with a preponderance of dominance component in genetic variance. A sub-set of 45 SSRs derived from C. olitorius, when used for a study of DNA polymorphism and genetic diversity, showed high transferability of these C. olitorius SSRs to C. capsularis. The average number of alleles for individual SSRs was surprisingly low (3.04 for both species, 2.02 for C. capsularis and 2.51 for C. olitorius), and so was the average polymorphic information content (PIC; 0.23 and 0.24 in two species). In the dendrogram obtained using a similarity matrix, the 81 genotypes were grouped into three clusters, which largely corresponded to the two species, Cluster I belonging mainly to C. capsularis and the other two closely related clusters (clusters II and III) belonging to C. olitorius. It was also shown that a minimum of 15 SSRs could give the same information as 41 SSRs, thus making many SSRs redundant. The SSR markers developed during the present study and to be developed in future will prove useful not only for evaluation of genetic diversity, but also for molecular mapping/QTL analysis, and for comparative genome analysis of the two Corchorus species. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
32. NMR Assignment of the Holo-ACP from Malaria Parasite Plasmodium Falciparum.
- Author
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Sharma, Alok Kumar, Sharma, Shailendra Kumar, Surolia, Namita, and Sarma, Siddhartha P.
- Subjects
LETTERS to the editor ,NUCLEAR magnetic resonance ,PEPTIDES ,CARRIER proteins ,MALARIA ,PLASMODIUM falciparum - Abstract
Presents letters to the editor discussing nuclear magnetic resonance assignments of peptide complex and holo-acyl carrier protein from malaria parasite Plasmodium falciparum. Discussion on Bcl-2 family of proteins; Role of peptides derived from the BH3 domains of pro-apoptotic Bcl-2 family members; Function of acyl carrier proteins in fatty acid biosynthesis.
- Published
- 2005
- Full Text
- View/download PDF
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