1. Hepatokine Fetuin B expression is regulated by leptin-STAT3 signalling and associated with leptin in obesity.
- Author
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Wang, Dongmei, Wu, Menghua, Zhang, Xiaofang, Li, Long, Lin, Mingzhu, Shi, Xiulin, Zhao, Yan, Huang, Caoxin, and Li, Xuejun
- Subjects
ALPHA fetoproteins ,LEPTIN ,ADIPOSE tissues ,METABOLIC disorders ,ADIPOSE tissue physiology ,CELLULAR signal transduction ,INSULIN resistance - Abstract
Obesity is an expanding global public health problem and a leading cause of metabolic disorders. The hepatokine Fetuin B participates in regulating insulin resistance, glucose metabolism and liver steatosis. However, the mechanism underlying Fetuin B activation remains unclear. Our previous population-based study demonstrated a significant association between serum Fetuin B and body fat mass in an obese population, which indicates its potential in mediating obesity-related metabolic disorders. In the present study, we further revealed a significant correlation between Fetuin B and leptin, the classic adipokine released by expanding adipose tissue, in this obese population. Consistently, elevated Fetuin B and leptin levels were confirmed in diet-induced obese mice. Furthermore, an in vitro study demonstrated that the leptin signalling pathway directly activated the transcription and expression of Fetuin B in primary hepatocytes and AML12 cells in a STAT3-dependent manner. STAT3 binds to the response elements on FetuB promoter to directly activate FetuB transcription. Finally, the mediating effect of Fetuin B in insulin resistance induced by leptin was confirmed according to mediation analysis in this obese population. Therefore, our study identifies leptin-STAT3 as an upstream signalling pathway that activates Fetuin B and provides new insights into the pathogenic mechanisms of obesity-related metabolic disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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