Author: "Shirokov, Vladimir" / Publisher: springer nature - Searchworks@Jio Institute Digital Library Search Results

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Start Over Author "Shirokov, Vladimir" Publisher springer nature

Author: Schneider, Birgit, Junge, Friederike, Shirokov, Vladimir A., Durst, Florian, Schwarz, Daniel, Dötsch, Volker, and Bernhard, Frank
Published: 2010
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Author: Reckel, Sina, Sobhanifar, Solmaz, Durst, Florian, Löhr, Frank, Shirokov, Vladimir A., Dötsch, Volker, and Bernhard, Frank
Published: 2010
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Author: Walker, John M., Grandi, Guido, Shirokov, Vladimir A., Kommer, Aigar, Kolb, Vyacheslav A., and Spirin, Alexander S.
Abstract: Protein synthesis in cell-free systems is an emerging technology already competing with in vivo expression methods. In this chapter the basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat germ translation and transcription-translation systems are described. The ways to improve substrate supply in cell-free systems and mRNA design for eukaryotic system are discussed. Correct folding of the synthesized protein is demonstrated and discussed in detail. [ABSTRACT FROM AUTHOR]
Published: 2007
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Author: Myasnikov, Alexander G., Afonina, Zhanna A., Ménétret, Jean-François, Shirokov, Vladimir A., Spirin, Alexander S., and Klaholz, Bruno P.
Published: 2014
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