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The point load strength test index (Is(50)) rapidly determines the tensile strength and the uniaxial compressive strength of rocks. It is necessary to analyze the power index (m) of Is(50) when conducting a point load test (PLT) on irregular rock blocks. In this study, sandy dolomites from the Sinian Dengying Formation (Zbdn) and Doushantuo Formation (Zbd) in the Yuxi section of the Central Yunnan Water Diversion Project were analyzed for their physical and mechanical properties. PLTs were performed on dolomites with different geological origins and weathering degrees, and the m-values were determined through statistical methods and regression analysis. The results showed that the logP–logDe2 curve exhibits a negative slope, and the m-value and its correlation coefficient (R2) increased with the decreasing weathering degree. For similar weathering conditions, the R2 of the Zbdn dolomites was higher than that of the Zbd dolomites. The error analysis indicated that the obtained m-values were highly accurate for the dolomite with different geological origins and weathering degrees. Casting slice identification tests further confirmed that the dolomite pore structure and density, connectedness, and specific mineral composition were the primary factors influencing the m-value. Highlights: This is the first study on the power index (m) of sandy dolomites. The logP–logDe2 curve exhibits a negative slope, and m-value and its R2 increase with decreasing weathering degree. Pore structure and density, connectedness, and specific mineral composition primarily influence the m-value. For a similar weathering degree, the R2 of Zbdn dolomites was higher than that of Zbd dolomites. [ABSTRACT FROM AUTHOR]

At present, epigenetic markers have been extensively studied in various fields and have a high value in forensic medicine due to their unique mode of inheritance, which does not involve DNA sequence alterations. As an epigenetic phenomenon that plays an important role in gene expression, non-coding RNAs (ncRNAs) act as key factors mediating gene silencing, participating in cell division, and regulating immune response and other important biological processes. With the development of molecular biology, genetics, bioinformatics, and next-generation sequencing (NGS) technology, ncRNAs such as microRNA (miRNA), circular RNA (circRNA), long non-coding RNA (lncRNA), and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) are increasingly been shown to have potential in the practice of forensic medicine. NcRNAs, mainly miRNA, may provide new strategies and methods for the identification of tissues and body fluids, cause-of-death analysis, time-related estimation, age estimation, and the identification of monozygotic twins. In this review, we describe the research progress and application status of ncRNAs, mainly miRNA, and other ncRNAs such as circRNA, lncRNA, and piRNA, in forensic practice, including the identification of tissues and body fluids, cause-of-death analysis, time-related estimation, age estimation, and the identification of monozygotic twins. The close links between ncRNAs and forensic medicine are presented, and their research values and application prospects in forensic medicine are also discussed. [ABSTRACT FROM AUTHOR]

Background: Human aortic vascular smooth muscle cells (HA-VSMCs) play vital roles in the pathogenesis of vascular diseases. Circular RNAs (circRNAs) have been reported to regulate the biological functions of HA-VSMCs. In this study, the functions of circRNA pecanex homolog (circPCNX) in platelet-derived growth factor-BB (PDGF-BB)-induced HA-VSMCs were investigated. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to determine the expression of circPCNX, DNA methyltransferase 1 (DNMT1), and microRNA-1278 (miR-1278). 5′-Ethynyl-2′-deoxyuridine (EdU) assay, flow cytometry analysis, wound healing assay, and transwell assay were used to examine cell proliferation, cell cycle, and migration. Western blot assay was utilized to measure protein levels. RNA immunoprecipitation (RIP) assay, RNA pull down assay, and dual-luciferase reporter assay were adopted to analyze the relationships among circPCNX, miR-1278, and DNMT1. Results: CircPCNX was upregulated in PDGF-BB-treated HA-VSMCs in a dose- or time-dependent manner. CircPCNX knockdown alleviated PDGF-BB-induced cell proliferation, cell cycle progression, and migration in HA-VSMCs. CircPCNX knockdown could reverse PDGF-BB-induced HA-VSMC progression by regulating DNMT1. Moreover, circPCNX was identified to regulate DNMT1 expression by sponging miR-1278. Inhibition of miR-1278 reversed circPCNX knockdown-mediated effects on cell proliferation and migration in PDGF-BB-induced HA-VSMCs. MiR-1278 overexpression suppressed PDGF-BB-stimulated HA-VSMC proliferation and migration by targeting DNMT1. Conclusion: CircPCNX promoted PDGF-BB-induced HA-VSMC proliferation and migration by elevating DNMT1 expression through sponging miR-1278. [ABSTRACT FROM AUTHOR]

To investigate the impact of permeability and heterogeneity on oil displacement efficiency and remaining oil distribution of chemical flooding, three groups of high and ultrahigh permeability core samples from an ultrahigh water-cut oilfield in western China were selected as the research objects in this study. High-pressure mercury injection, scanning electron microscopy, wettability test, and other methods were used to characterize the reservoir properties of core samples. Six groups of experiments were performed using the nuclear magnetic resonance (NMR) displacement imaging technology to simulate the oilfield development process considering the economic benefits. The displacement stage with the best oil displacement effect in the process of waterflooding, chemical flooding and then waterflooding was defined, and the control effect of permeability and heterogeneity on the improvement of oil displacement efficiency by polymer-surfactant binary flooding was discussed. The distribution position of remaining oil in different displacement stages was quantitatively and visually displayed, and its control factors were revealed. The research shows that during the simulation process of first waterflooding followed by chemical flooding and then waterflooding in the oilfield, the T2 spectrum signal amplitude increases the most in the two stages, one is from saturated oil flooding to 50% water cut and the other one is from 95% water cut to the end of 1 PV polymer flooding. The oil displacement efficiency increases the most, and the oil is primarily discharged from pore throats larger than 90 ms (or with pore throat radius of 8.37 μm). Compared with heterogeneity, permeability plays a more obvious controlling role in improving the oil displacement efficiency of polymer-surfactant binary flooding. The influence of fingering phenomenon on the distribution of remaining oil is most obvious in the second waterflooding, and the distribution of remaining oil with polymer slug is more obviously affected by the fingering phenomenon than that with polymer-surfactant slug. The study results provide theoretical guidance for tapping the remaining oil potential of old oilfields with high to ultrahigh permeabilities. [ABSTRACT FROM AUTHOR]

Background: BSG (CD147) is a member of the immunoglobulin superfamily that shows roles for potential prognostics and therapeutics for metastatic cancers and SARS-CoV-2 invasion for COVID-19. The susceptibility of malignant cancers to SARS-CoV-2 as well as the correlations between disease outcome and BSG expression in tumor tissues have not been studied in depth. Methods: In this study, we explored the BSG expression profile, survival correlation, DNA methylation, mutation, diagnostics, prognostics, and tumor-infiltrating lymphocytes (TILs) from different types of cancer tissues with corresponding healthy tissues. In vitro studies for cordycepin (CD), N6-(2-hydroxyethyl) adenosine (HEA), N6, N6-dimethyladenosine (m62A) and 5′-uridylic acid (UMP) on BSG expression were also conducted. Results: We revealed that BSG is conserved among different species, and significantly upregulated in seven tumor types, including ACC, ESCA, KICH, LIHC, PAAD, SKCM and THYM, compared with matched normal tissues, highlighting the susceptibility of these cancer patients to SARS-CoV-2 invasion, COVID-19 severity and progression of malignant cancers. High expression in BSG was significantly correlated with a short OS in LGG, LIHC and OV patients, but a long OS in KIRP patients. Methylation statuses in the BSG promoter were significantly higher in BRCA, HNSC, KIRC, KIRP, LUSC, PAAD, and PRAD tumor tissues, but lower in READ. Four CpGs in the BSG genome were identified as potential DNA methylation biomarkers which could be used to predict malignant cancers from normal individuals. Furthermore, a total of 65 mutation types were found, in which SARC showed the highest mutation frequency (7.84%) and THYM the lowest (0.2%). Surprisingly, both for disease-free and progression-free survival in pan-cancers were significantly reduced after BSG mutations. Additionally, a correlation between BSG expression and immune lymphocytes of CD56bright natural killer cell, CD56dim natural killer cell and monocytes, MHC molecules of HLA-A, HLA-B, HLA-C and TAPBP, immunoinhibitor of PVR, PVRL2, and immunostimulators of TNFRSF14, TNFRSF18, TNFRSF25, and TNFSF9, was revealed in most cancer types. Moreover, BSG expression was downregulated by CD, HEA, m62A or UMP in cancer cell lines, suggesting therapeutic potentials for interfering entry of SARS-CoV-2. Conclusions: Altogether, our study highlights the values of targeting BSG for diagnostic, prognostic and therapeutic strategies to fight malignant cancers and COVID-19. Small molecules CD, HEA, m62A and UMP imply therapeutic potentials in interfering with entry of SARS-CoV-2 and progression of malignant cancers. [ABSTRACT FROM AUTHOR]

Heat Shock Protein Family A (Hsp70) Member 6 (HSPA6) (Online Mendelian Inheritance in Man: 140555) belongs to the HSP70 family and is a partially conserved inducible protein in mammals. The HSPA6 gene locates on the human chromosome 1q23.3 and encodes a protein containing two important structural domains: The N-terminal nucleotide-binding domain and the C-terminal substrate-binding domain. Currently, studies have found that HSPA6 not only plays a role in the tumorigenesis and tumor progresses but also causes non-tumor-related diseases. Furthermore, HSPA6 exhibits to inhibit tumorigenesis and tumor progression in some types of cancers but promotes in others. Even though HSPA6 research has increased, its exact roles and mechanisms are still unclear. This article reviews the structure, expression, function, research progress, possible mechanism, and perspective of HSPA6 in cancers and other diseases, highlighting its potential role as a targeted therapeutic and prognostic marker. [ABSTRACT FROM AUTHOR]

The in situ pore pressure response of silt under wave action is a complex process. However, this process has not been well studied because of limited field observation techniques. The dynamic response process is closely related to engineering geological hazards; thus, this process must be urgently explored. A long-term in situ observational study of the silt sediment pore water pressure response process under wave action was conducted in the subaqueous Yellow River Delta. The response characteristics of pore water pressure are affected by tidal level and wave height. Tidal level affects the overall trend of the pore water pressure response, while wave height influences the amplitude of the pore water pressure response. This study revealed a significant lag effect in the pore pressure response. The transient pore pressure in the seabed did not respond immediately to the wave-induced pressure stress on the seabed surface. This phenomenon may be attributed to the change in soil permeability. The maximum response depth was approximately 0.5 m with a 2 m wave height. A concept model of silt soil pore pressure response under different types of wave action was developed. The accumulation rate of the pore pressure is less than the dissipation rate; thus, the developed model highlights the oscillation pore pressure response mechanism. The highlighted response process is of considerable importance to transient liquefaction and the startup process of pore pressure response. [ABSTRACT FROM AUTHOR]

The Yi is one of fifty-six ethnic populations and one of the most ancient ethnic groups in China. The Liangshan Yi Autonomous Prefecture (LYAP) in Sichuan Province has the single largest Yi community in China. To establish a Yi population database in the LYAP of Sichuan in China, a Goldeneye™ DNA Identification System 20A Kit with 19 autosomal STRs (short tandem repeats) was used. As a result, the total discrimination power (TDP) and the cumulative probability of exclusion (CPE) for these STRs in 1016 unrelated individuals were 0.999999999999999999999897 and 0.9999999597, respectively. Totals of 273 alleles for 19 STRs and 8–22 alleles for each locus were found. The allelic frequencies ranged from 0.0005 to 0.5084. The forensic parameter averages of these STRs were as follows: observed heterozygosity (Hobs) of 78.44%, expected heterozygosity (Hexp) of 79.89%, discrimination power (DP) of 92.66%, and probability of exclusion (PE) of 57.68%. Penta E presented the highest levels of Hobs and DP, whereas TPOX showed the lowest Hobs and DP values. Nei's standard genetic distance matrix among 31 populations found that the nearest genetic distance to the Yi population was the Sichuan Han (0.0056). Altogether, we first reported the forensic parameters and allele frequencies of 19 autosomal STRs of the Yi group in Liangshan. These 19 STR makers could provide highly informative polymorphisms for individual identification, paternity testing and genetic population analyses. [ABSTRACT FROM AUTHOR]

To clarify the sliding mechanism of a landslide, the shear behavior characteristics of slip zone soil are always vital. In the Northwest of China, thousands of landslides generally develop in three main types of slip zone soil, i.e., Quaternary loess, weathered Mesozoic-Cenozoic mudstone, and weathered Mesozoic-Paleozoic phyllite. In the present study, the shear behavior characteristics of these three kinds of slip zone soil were investigated through direct shear tests and their implications for the sliding mechanisms of landslide were also discussed. It was found that, in general, an abrupt failure of loess landslide could be related to the strong strain-softening behavior of loess in the direct shear test, while the ductile shear behavior of weathered mudstone and phyllite coincided with the creep deformation of the corresponding landslides. The residual friction angle of slip zone soil varied with the soil type and its mineral composition, and the lowest residual friction angle about 11.5° could be found in weathered phyllite (SET) with graphite. Compared with the previous research, it is interesting to find that the residual friction angles of mudstone and phyllite in the present study accorded reasonably with the empirical correlations proposed by Wen et al. (2007) for weak rocks in the Three Gorges area, China. [ABSTRACT FROM AUTHOR]

The exploration undertaken by the Chinese writer Gao Xingjian is among the most representative of the drama experiments made in contemporary China. Inspired by avant-garde Western playwrights, especially Samuel Beckett and Bertolt Brecht, Gao transcended the realistic tradition of modern Chinese drama throughout his forty-year career. Brecht's misinterpretation of Chinese drama enabled Gao to transform the writing and performance of traditional Chinese drama in his own original way, which opened up a uniquely oriental approach to drama explorations worldwide. [ABSTRACT FROM AUTHOR]