1. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
- Author
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Masato Nakagawa, Yukimasa Taniguchi, Sho Senda, Nanako Takizawa, Tomoko Ichisaka, Kanako Asano, Asuka Morizane, Daisuke Doi, Jun Takahashi, Masatoshi Nishizawa, Yoshinori Yoshida, Taro Toyoda, Kenji Osafune, Kiyotoshi Sekiguchi, and Shinya Yamanaka
- Subjects
EMBRYONIC stem cells ,PLURIPOTENT stem cells ,CONNECTIVE tissue cells ,HUMAN cloning ,FIBROBLASTS ,BLOOD cells - Abstract
In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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