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Osteosarcoma represents one of the most aggressive tumors of bone among adolescents and young adults. Despite improvements in treatment, osteosarcoma has a grave prognosis. The identification of prognostic factors is still in its infancy. Weighted gene correlation network analysis (WGCNA) was conducted on mRNA-sequencing and clinical information (gender, survival and metastasis) of osteosarcoma patients from the TARGET database to obtain genes in modules associated with metastasis of osteosarcoma. The Cox regression analysis was then performed on the gene expression profile from TARGET to screen genes associated with patients' survival. Known genes related to osteosarcoma were obtained by intersecting osteosarcoma-related genes from DisGeNET and DiGSeE, followed by the construction of PPI network of osteosarcoma-related genes and survival-related genes in modules. The screened key genes were subject to multi-factor Cox proportional hazards model, and osteosarcoma patients were classified into high- and low- risk groups according to the risk score to evaluate the potential of key genes to predict the survival of osteosarcoma patients. The WGCNA showed that 4 genes in tan and 19 genes in pink modules were related to the survival of osteosarcoma patients. Osteosarcoma-related known genes (9) were obtained in intersection of DisGeNET and DiGSeE. PPI network identified 4 key genes (KRT5, HIPK2, MAP3K5 and CD5) closely associated with survival of osteosarcoma patients. HIPK2, MAP3K5 and CD5 expression was inversely correlated with survival risk, while KRT5 expression was positively correlated with survival risk. These results show KRT5, HIPK2, MAP3K5 and CD5 serve as prognostic factors of osteosarcoma patients. [ABSTRACT FROM AUTHOR]

The structure and magnetic properties of Ce0.04Tm2.96CrxFe5-xO12 (x = 0–0.30) prepared via sol-gel route were studied in this paper. X-ray diffraction (XRD) data and Raman spectra (RS) data revealed that Cr and Ce strongly enter the Tm3Fe5O12 (TmIG) structure. RS results showed the characteristic peaks of TmIG garnets ferrites. The X-ray photoelectron spectroscopy (XPS) results in Ce0.04Tm2.96Cr0.25Fe4.75O12 revealed that single valence state of iron was "+3." Moreover, by comparing Ce0.04Tm2.96Fe5O12 with Cr-doped sample, saturation magnetization (Ms) was increased, which may be related to the fact that Cr3+ ions occupied octahedral position (a) to increase the effective moments formed by Fe3+ cations. The Ms of the sample x = 0.15 reaches a maximum of 18.24 emu/g. Furthermore, these nanoferrites with different magnetic properties and structures may be used for longitudinal recording and sensor applications. [ABSTRACT FROM AUTHOR]