4 results on '"Xiao, Yuansong"'
Search Results
2. Waterlogging resistance and evaluation of physiological mechanism of three peach (Prunus persica) rootstocks.
- Author
-
Zhang, Binbin, Sun, Maoxiang, Liu, Wenxin, Lian, Min, Yang, Sankui, Peng, Futian, and Xiao, Yuansong
- Subjects
- *
PEACH , *ROOTSTOCKS , *WATERLOGGING (Soils) , *CROP growth , *BILAYER lipid membranes - Abstract
Waterlogging occurs due to poor soil drainage or excessive rainfall. It is a serious abiotic stress factor that negatively affects crop growth. Waterlogging often causes plants to shed leaves, fruits, and, ultimately, to die. Peach (Prunus persica) trees are generally intolerant to waterlogging, and the primary peach rootstock used in Chinais "Maotao," which has very poor resistance to sensitivity. Therefore, waterlogging has become a restriction on the development of the peach industry in many regions. In this experiment, we tested the waterlogging resistance of "Maotao (Prunus persica (L.) Batsch)" (MT), "Shannong1 (GF677 × Cadaman)" (SN1), and "Mirabolano 29C (Prunus cerasifera)" (M29C) rootstocks. Using a simulated waterlogging method, the effects of waterlogging on the photosynthetic system, leaf pigments, osmotic adjustment, lipid membrane peroxidation, and antioxidant system of these three peach rootstocks were studied, and the changes of chlorophyll fluorescence parameters and fluorescence imaging were observed. The results showed that, with prolonged waterlogging, the photosynthetic pigment content and photosynthesis of the three peach rootstocks decreased rapidly, but the decomposition rate of SN1 and M29C chlorophyll was slower, and it still had high light energy absorption and energy transfer capabilities under waterlogging stress, which reduced the damage caused by waterlogging stress; under the stress of flooding, the osmoregulatory substances of the three rootstocks increased to varying degrees compared with normal conditions. At the same time, the enzyme activity of superoxide dismutase (SOD) activity, peroxidase (POD) activity, and catalase (CAT) activity in the leaves of the three rootstocks under flooding stress all increased and then decreased; during this period, malondialdehyde (MDA) continued to increase, and SN1 and M29C were significantly lower than MT; and chlorophyll fluorescence parameters, including the maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPSII), photochemical quenching coefficient (qP), non-photochemical quenching (NPQ), and electron transfer rate (ETR) decreased significantly. The tolerance of SN1 and M29C to waterlogging was significantly better than that of MT rootstocks. The rootstock and grafted seedlings of SN1 have good waterlogging tolerance. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. The effect of auxin and strigolactone on ATP/ADP isopentenyltransferase expression and the regulation of apical dominance in peach.
- Author
-
Li, MinJi, Wei, Qinping, Xiao, Yuansong, and Peng, FuTian
- Subjects
- *
AUXIN , *STRIGOLACTONES , *TRANSFERASES , *PEACH , *GENE expression in plants , *APICAL dominance (Plant physiology) - Abstract
Key message: We confirmed the roles of auxin, CK, and strigolactones in apical dominance in peach and established a model of plant hormonal control of apical dominance in peach.Abstract: Auxin, cytokinin, and strigolactone play important roles in apical dominance. In this study, we analyzed the effect of auxin and strigolactone on the expression of ATP/ADP isopentenyltransferase (IPT) genes (key cytokinin biosynthesis genes) and the regulation of apical dominance in peach. After decapitation, the expression levels of PpIPT1, PpIPT3, and PpIPT5a in nodal stems sharply increased. This observation is consistent with the changes in tZ-type and iP-type cytokinin levels in nodal stems and axillary buds observed after treatment; these changes are required to promote the outgrowth of axillary buds in peach. These results suggest that ATP/ADP PpIPT genes in nodal stems are key genes for cytokinin biosynthesis, as they promote the outgrowth of axillary buds. We also found that auxin and strigolactone inhibited the outgrowth of axillary buds. After decapitation, IAA treatment inhibited the expression of ATP/ADP PpIPTs in nodal stems to impede the increase in cytokinin levels. By contrast, after GR24 (GR24 strigolactone) treatment, the expression of ATP/ADP IPT genes and cytokinin levels still increased markedly, but the rate of increase in gene expression was markedly lower than that observed after decapitation in the absence of IAA (indole-3-acetic acid) treatment. In addition, GR24 inhibited basipetal auxin transport at the nodes (by limiting the expression of PpPIN1a in nodal stems), thereby inhibiting ATP/ADP PpIPT expression in nodal stems. Therefore, strigolactone inhibits the outgrowth of axillary buds in peach only when terminal buds are present. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
4. CEP55 3'-UTR promotes epithelial–mesenchymal transition and enhances tumorigenicity of bladder cancer cells by acting as a ceRNA regulating miR-497-5p.
- Author
-
Yang, Chenglin, Yang, Yue, Wang, Wei, Zhou, Wuer, Zhang, Xiaoming, Xiao, Yuansong, and Zhang, Huifen
- Subjects
- *
EPITHELIAL-mesenchymal transition , *BLADDER cancer , *CANCER cells , *ONCOGENIC proteins , *BINDING sites , *URODYNAMICS , *CELL migration inhibition - Abstract
Background: Centrosomal protein 55 (CEP55) is implicated in the tumorigenesis of bladder cancer (BC) but the detailed molecular mechanisms are unknown. We aim to develop a potential competing endogenous RNA (ceRNA) network related with CEP55 in BC. Methods: We first extracted the expression profiles of RNAs from The Cancer Genome Atlas (TCGA) database and used bioinformatic analysis to establish ceRNAs in BC. Real-time quantity PCR (RT-qPCR) and immunohistochemical analysis were performed to measure CEP55 expression in different bladder cell lines and different grades of cancer. Bioinformatics analysis and luciferase assays were conducted to predict potential binding sites among miR-497-5p, CEP55, parathyroid hormone like hormone (PTHLH) and high mobility group A2 (HMGA2). Tumor xenograft model was used to show the effect of CEP55 3'-UTR on cisplatin therapy. Bioinformatics analysis, luciferase assays, and 5' rapid amplification of cDNA ends (5'RACE) were to explore the function of CEP55 3'-untranslated region (3'-UTR) on targeting miR-497-5p. Western blot and immunofluorescence assays were to detect the epithelial–mesenchymal transition (EMT) induction of CEP55 3'-UTR. Results: CEP55 expression as well as the expression levels of the oncogenic proteins PTHLH and HMGA2 were upregulated in BC cells while miR-497-5p was downregulated. Low miR-497-5p expression and high CEP55 and HMGA2 expression levels were associated with more advanced tumor clinical stage and pathological grade. Overexpression of the CEP55 3'-UTR promoted the proliferation, migration, and invasion of the EJ cell line in vitro and accelerated EJ-derived tumor growth in nude mice, while inhibition of the CEP55 3'-UTR suppressed all of these oncogenic processes. In addition, CEP55 3'-UTR upregulation reduced the cisplatin sensitivity of BC cell lines and xenograft tumors. Bioinformatics analysis, luciferase assays, and 5'RACE suggested that the CEP55 3'-UTR functions as a ceRNA targeting miR-497-5p, leading to miR-497-5p downregulation and disinhibition of PTHLH and HMGA2 expression. Further, CEP55 downregulated miR-497-5p transcription by promoting NF- κ B signaling. In turn, CEP55 3'-UTR ultimately promotes EMT and tumorigenesis by activating P38MAPK and ERK 1/2 pathways. Conclusions: These results suggest that a ceRNA regulatory network involving CEP55 upregulates PTHLH and HMGA2 expression by suppressing endogenous miR-497-5p. We unveiled a novel mechanism of BC metastasis, and could become novel therapeutics targets in BC. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.