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Abstract  Wolfram syndrome (WFS), also known as DIDMOAD, is an infrequent cause of diabetes mellitus. WFS is an autosomal recessive neurodegenerative disease characterized by various clinical manifestations such as diabetes mellitus, optic atrophy, diabetes insipidus, deafness, neurological symptoms, renal tract abnormalities, psychiatric disorders, and gonadal disorders. The majority of patients with WFS carry the loss of function mutations in the WFS1 gene. The exons 2–8 of the WFS1 gene from one Chinese WFS patient were amplified by the polymerase chain reaction (PCR), subcloning techniques and direct sequence determination was applied to the amplified fragments. The compound heterozygous mutation of a 3-bp (GAC) deletion (V434del) and another compound heterozygous mutation (G→N)(W666X) in exon 8 of WFS1 gene was identified in the patient. Other seventeen members of her family were investigated. Four cases with heterozygotes had been found through screening for the mutation V434del and five cases for the mutation W666X in the whole family. This is the first report of WFS with the mutation V434del and W666X in the WFS1 gene. [ABSTRACT FROM AUTHOR]

A sub-micrometer thin-layer DNA modified carbon fiber microcylinder electrode was prepared by electrodeposition of ct-DNA at 1.5 V (vs. Ag/AgCl). The voltammetric behavior of dopamine (3-hydroxytyramine) was investigated at the modified electrode. It was found that the modified electrode exhibits a highly electrocatalytic activity toward dopamine oxidation. Differential pulse voltammetry was used for determination of dopamine in pH 7.4 phosphate buffer solution. A linear response of the peak current versus the concentration was found in the range of 4 × 10-6 to 10-4 mol L-1 at 10-4 mol L-1 AA (ascorbic acid) coexistence (R = 0.9959) and the range of 6 × 10-5 to 10-3 mol L-1 at 10-3 mol L-1 AA (R = 0.9960). The presence of a high concentration of ascorbic acid did not interfere with the determination. The proposed method exhibited good recovery and reproducibility. This method can be applied to the detection of DA in real samples. [ABSTRACT FROM AUTHOR]

Provides information on a study which examined the effects and possible molecular target of a specific cyclo-oxygenase-2 (COX-2) inhibitor in gastric cancer. Utilization of nonsteroidal anti-inflammatory drugs in patients with gastrointestinal cancers; Mechanism of COX-2 inhibitors; Discussion of the process of apoptosis and cancer cell growth.