1. Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.
- Author
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Ghanaeian, Avrin, Majhi, Sumita, McCafferty, Caitlyn L., Nami, Babak, Black, Corbin S., Yang, Shun Kai, Legal, Thibault, Papoulas, Ophelia, Janowska, Martyna, Valente-Paterno, Melissa, Marcotte, Edward M., Wloga, Dorota, and Bui, Khanh Huy
- Subjects
CELL motility ,EUKARYOTIC cells ,CILIA & ciliary motion ,CELL communication ,TUBULINS ,TETRAHYMENA - Abstract
Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity. Motile cilia are hair-like structures that are found on the surface of eukaryotic cells providing cell motility. Here, authors reveal the twelve components of nexin-dynein regulatory complex and associated proteins in cilia from Tetrahymena thermophila. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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