1. Characterization and genome annotation of a newly detected bacteriophage infecting multidrug-resistant Acinetobacter baumannii.
- Author
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Yang, Zichen, Liu, Xinzhu, Shi, Yunlong, Yin, Supeng, Shen, Wei, Chen, Jing, Chen, Yu, Chen, Yajie, You, Bo, Gong, Yali, Luo, Xiaoqiang, Zhang, Cheng, Yuan, Zhiqiang, and Peng, Yizhi
- Subjects
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ACINETOBACTER baumannii , *BACTERIOPHAGES , *CIRCULAR DNA , *SEWAGE purification , *TRANSMISSION electron microscopy , *NUCLEOTIDE sequencing - Abstract
A novel virulent bacteriophage, φAbp2, infecting multidrug-resistant (MDR) Acinetobacter baumannii was isolated from the wastewater of a sewage management centre at Southwest Hospital, China. Transmission electron microscopy and phylogenetic analysis revealed that φAbp2 belongs to the subfamily Peduovirinae. A one-step growth curve demonstrated that φAbp2 had a latent period of 15 min, a lysis period of 35 min, and a burst size of 222 particles per infected host cell. Moreover, φAbp2 showed a relatively broad host range in local A. baumannii, and it also exhibited tolerance over a wider range of thermal and pH conditions. Genomic sequencing revealed that φAbp2 has a circular double-stranded DNA genome with no sequence similarity to our previously isolated φAbp1. Eighty-eight putative open reading frames (ORFs) encoding 41 proteins of known function and 47 of unknown function were identified, and the G/C content was 37.84%. φAbp2 is a new member of the subfamily Peduovirinae of the family Myoviridae. Its genome sequence is very similar to that of the A. baumannii phage LZ35. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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